Circulating perivascular progenitors: a target of PDGFR inhibition.


:Cancer blood vessels consist of two interacting types of cells: inner lining endothelial cells (ECs) and surrounding perivascular cells (pericytes, vascular smooth muscle cells or mural cells). PDGFRbeta(CD140b)+ progenitor perivascular cells (PPC) can differentiate into pericytes and regulate vessel stability and vascular survival in tumors. Similarly to what we have done with circulating ECs and progenitors, we developed a flow cytometry procedure for the enumeration of circulating PPCs and the study of their viability in murine models of cancer and in cancer patients. DNA+CD45-CD31-CD140b+ cells were enumerated by six-colour flow cytometry, their morphology was studied by electron microscopy, PPC specificity confirmed by reverse trascription-PCR (RT-PCR) expression of CD140b mRNA, and viability assessed by Syto16 and 7AAD. In preclinical marrow transplantation studies, 9 ± 4% of circulating PPCs were derived from the marrow donor. PPCs were increased in cancer-bearing mice and in patients affected by some types of cancer. At variance with the kinetic of circulating endothelial progenitors, high-dose cyclophosphamide reduced the number of viable PPCs. The administration of sunitinib, a drug known to inhibit PDGFR, was associated in murine models and in cancer patients with an increase of apoptotic/necrotic circulating PPC, suggesting a direct targeting of these cells. PPC enumeration might be studied as a tool for the definition of the optimal biologic dose of anti-PDGFR drugs and investigated clinically as a possible predictive/prognostic tool in patients receiving anti-PDGFR drugs.


Int J Cancer


Mancuso P,Martin-Padura I,Calleri A,Marighetti P,Quarna J,Rabascio C,Braidotti P,Bertolini F




Has Abstract


2011-09-15 00:00:00












  • Evidence for alternative lengthening of telomeres in liposarcomas in the absence of ALT-associated PML bodies.

    abstract::Immortalized and cancer cells maintain their telomeres by activation of a telomere maintenance mechanism (TMM). In approximately 85% of cancers telomerase is activated (TA) but in some tumours, in particular sarcomas, an alternative lengthening of telomeres (ALT) pathway is used. Liposarcomas are the most common soft-...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Jeyapalan JN,Mendez-Bermudez A,Zaffaroni N,Dubrova YE,Royle NJ

    更新日期:2008-06-01 00:00:00

  • Effects of an inducible anti-sense c-myc gene transfer in a drug-resistant human small-cell-lung-carcinoma cell line.

    abstract::Small-cell-lung-cancer (SCLC) is characterized by rapid development of resistance to cytotoxic agents, such as cisplatin (cDDP) and anthracyclines. c-myc over-expression is one of the reported genetic alterations in this tumor. Amplification of the c-myc gene in this and other cancers is often correlated with poor pro...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Van Waardenburg RC,Meijer C,Burger H,Nooter K,De Vries EG,Mulder NH,De Jong S

    更新日期:1997-11-14 00:00:00

  • Dynamic analysis of circulating tumor DNA to predict prognosis and monitor therapeutic response in metastatic relapsed cervical cancer.

    abstract::Limited and inefficient treatment options exist for metastatic relapsed cervical cancer (MRCC), and there are currently no reliable indicators to guide therapeutic selection. We performed deep sequencing analyses targeting 322 cancer-related genes in plasma cell-free DNA and matched white blood cells in 173 serial blo...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tian X,Ge D,Zhang F,Zhang B,Bai W,Xu X,Li Z,Cao Y,Li P,Zou K,Zou L

    更新日期:2021-02-15 00:00:00

  • Characteristic chromosomal abnormalities in biopsies and lymphoid-cell lines from patients with Burkitt and non-Burkitt lymphomas.

    abstract::The karyotypes of cells from 10 Burkitt lymphoma (BL) biopsies, eight cell lines established from BL and nine cell lines from non-BL sources were studied by chromosome banding techniques. With the exception of the BL-derived cell lines BJAB, GC-BJAB, Maku and U-8691 all biopsies and lines of Burkitt origin contained a...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Zech L,Haglund U,Nilsson K,Klein G

    更新日期:1976-01-15 00:00:00

  • Effects of the farnesyltransferase inhibitor UCF-1C/manumycin on growth and p21-ras post-translational processing in NIH3T3 cells.

    abstract::Examination of the effect of the farnesylprotein transferase (FPTase) inhibitor UCF1-C/manumycin on NIH3T3 cells transfected with a normal N-ras gene and expressing high levels of the corresponding p21-ras protein showed that 10 microm UCF1-C immediately and reversibly inhibited growth in these cells, without modifyin...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Servais P,Gulbis B,Fokan D,Galand P

    更新日期:1998-05-18 00:00:00

  • Ultrastructural evidence of collagenolytic activity in ductal infiltrating carcinoma of the human breast.

    abstract::The stroma of ductal infiltrating carcinoma of the human breast shows characteristic and localized areas of collagen rarefaction and fragmentation. This finding has been correlated with a peculiar type of fibrillar damage, observed in a small percentage of collagen fibrils isolated in the native state from the tumour ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pucci-Minafra I,Luparello C,Schillaci R,Sciarrino S

    更新日期:1987-05-15 00:00:00

  • The gene for the cyclin-dependent-kinase-4 inhibitor, CDKN2A, is preferentially deleted in malignant mesothelioma.

    abstract::Cytogenetic deletions of the short arm of chromosome 9, 9p, have been detected in cell lines of malignant mesothelioma as well as in tumor material. Many tumor types carry deletions of chromosome 9 or more specifically of 9p21. The tumor-suppressor genes, CDKN2A and CDKN2B, each of which encodes a structurally and fun...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Prins JB,Williamson KA,Kamp MM,Van Hezik EJ,Van der Kwast TH,Hagemeijer A,Versnel MA

    更新日期:1998-02-09 00:00:00

  • Age- and sex-specific cumulative rate and risk of ATLL for HTLV-I carriers.

    abstract::We have surveyed the incidence of adult T-cell leukemia/lymphoma (ATLL) in an endemic area of 290,464 inhabitants for 7 years. We now revise our previous results on the basis of additional findings and estimate the age- and sex-specific cumulative rate for HTLV-I carriers, the adoption of which is recommended by curre...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kondo T,Kono H,Miyamoto N,Yoshida R,Toki H,Matsumoto I,Hara M,Inoue H,Inatsuki A,Funatsu T

    更新日期:1989-06-15 00:00:00

  • Transcriptomic profiling reveals three molecular phenotypes of adenocarcinoma at the gastroesophageal junction.

    abstract::Cancers occurring at the gastroesophageal junction (GEJ) are classified as predominantly esophageal or gastric, which is often difficult to decipher. We hypothesized that the transcriptomic profile might reveal molecular subgroups which could help to define the tumor origin and behavior beyond anatomical location. The...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bornschein J,Wernisch L,Secrier M,Miremadi A,Perner J,MacRae S,O'Donovan M,Newton R,Menon S,Bower L,Eldridge MD,Devonshire G,Cheah C,Turkington R,Hardwick RH,Selgrad M,Venerito M,Malfertheiner P,OCCAMS Consortium.,F

    更新日期:2019-12-15 00:00:00

  • Telomerase reverse transcriptase mutations are independent predictor of disease-free survival in Middle Eastern papillary thyroid cancer.

    abstract::Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. Tumor recurrence occurs in ∼20% of PTCs and some reach advanced stages. Promoter mutation in the telomerase reverse transcriptase (TERT) gene is identified to be a prognostic marker in PTC. However, the contribution of TERT promoter mutation ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bu R,Siraj AK,Divya SP,Kong Y,Parvathareddy SK,Al-Rasheed M,Al-Obaisi KAS,Victoria IG,Al-Sobhi SS,Al-Dawish M,Al-Dayel F,Al-Kuraya KS

    更新日期:2018-05-15 00:00:00

  • Nonsteroidal anti-inflammatory drugs and risk of lung cancer.

    abstract::Regular aspirin and non-aspirin nonsteroidal anti-inflammatory drug (NSAID) use is associated with a reduced risk of colorectal cancer. The effect of NSAIDs on the risk of other cancers remains unclear. To evaluate whether use of aspirin or other specific NSAIDs protects against lung cancer, we conducted a case-contro...

    journal_title:International journal of cancer

    pub_type: 杂志文章,随机对照试验


    authors: Hernández-Díaz S,García Rodríguez LA

    更新日期:2007-04-01 00:00:00

  • Acrylamide exposure measured by food frequency questionnaire and hemoglobin adduct levels and prostate cancer risk in the Cancer of the Prostate in Sweden Study.

    abstract::Acrylamide, a probable human carcinogen, is formed during the cooking of many commonly consumed foods. Data are scant on whether dietary acrylamide represents an important cancer risk in humans. We studied the association between acrylamide and prostate cancer risk using 2 measures of acrylamide exposure: intake from ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wilson KM,Bälter K,Adami HO,Grönberg H,Vikström AC,Paulsson B,Törnqvist M,Mucci LA

    更新日期:2009-05-15 00:00:00

  • Expression of Epstein-Barr virus in cutaneous T-cell lymphoma including mycosis fungoides.

    abstract::Cutaneous T-Cell lymphoma (CTCL) is a non-Hodgkin's lymphoma of unknown pathogenesis. Mycosis fungoides (MF) is a clinically determined subset of CTCL with intensive infiltration of lymphoma cells into the epidermis. To determine whether Epstein-Barr virus (EBV) is associated with these lymphoma cells, we performed mR...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Shimakage M,Sasagawa T,Kawahara K,Yutsudo M,Kusuoka H,Kozuka T

    更新日期:2001-04-15 00:00:00

  • The sialosyl Lewis a ganglioside is present in tumorigenic human urothelial cell lines.

    abstract::The sialosyl Lewis a antigen was identified in a ganglioside extract from a malignant human urothelial cell line (Hu 1703He) by fast atom bombardment mass spectrometry. The presence of this antigen in urothelial cell lines with varying tumorigenic properties was further studied using the 19-9 monoclonal antibody (MAb)...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ugorski M,Påhlsson P,Dus D,Nilsson B,Skouv J,Radzikowski C

    更新日期:1990-06-15 00:00:00

  • Functional impact of sequence variation in the promoter region of TGFB1.

    abstract::Pathological deregulation of the transforming growth factor, beta 1 (TGFB1) pathway has been implicated in the development of several major diseases, including cancers. Regulatory variation in the TGFB1 gene may lead to altered TGFB1 expression and activity, and thus, modulate an individual's susceptibility to disease...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Healy J,Dionne J,Bélanger H,Larivière M,Beaulieu P,Labuda D,Sinnett D

    更新日期:2009-09-15 00:00:00

  • Alcohol drinking, body mass index and the risk of oral leukoplakia in an Indian population.

    abstract::Although tobacco habits have been associated with the risk of oral leukoplakia, alcohol drinking and body mass index (BMI) as risk factors have not been well established. The purpose of this study is to evaluate the independent effects of drinking, BMI, tobacco chewing and smoking on the risk of oral leukoplakia. A ca...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hashibe M,Sankaranarayanan R,Thomas G,Kuruvilla B,Mathew B,Somanathan T,Parkin DM,Zhang ZF

    更新日期:2000-10-01 00:00:00

  • Analysis of normal neoplastic human tissues for the tumor-associated protein p97.

    abstract::We have used immunoprecipitation to test tumor biopsies and normal adult and fetal human tissues for p97, a tumor-associated protein. Five of nine melanoma biopsies contained p97 in low to very high levels. Three of seven non-melanoma tumors contained p97, but in smaller amounts. No p97 was detected in any of the norm...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Woodbury RG,Brown JP,Loop SM,Hellström KE,Hellström I

    更新日期:1981-02-15 00:00:00

  • A higher ctDNA fraction decreases survival in regorafenib-treated metastatic colorectal cancer patients. Results from the regorafenib's liquid biopsy translational biomarker phase II pilot study.

    abstract::The predictive effect of circulating tumor DNA (ctDNA) in colorectal cancer (CRC) treatment is still highly discussed. The primary objective of our study was to investigate a possible prognostic/predictive value of ctDNA under regorafenib treatment. This prospective multicenter translational biomarker phase II pilot s...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Unseld M,Belic J,Pierer K,Zhou Q,Moser T,Bauer R,Piringer G,Gerger A,Siebenhüner A,Speicher M,Heitzer E,Prager GW

    更新日期:2021-03-15 00:00:00

  • Evidence of benefit from centralised treatment of ovarian cancer: a nationwide population-based survival analysis in Finland.

    abstract::To assess the effect of different hospital types or surgical volume on the survival of ovarian cancer patients, a nationwide and population-based analysis was carried out in Finland. The study included all 3,851 ovarian cancer patients operated from 1983-94. The patients were classified according to the hospital of th...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kumpulainen S,Grénman S,Kyyrönen P,Pukkala E,Sankila R

    更新日期:2002-12-10 00:00:00

  • Factors that influence the penetration of methotrexate through solid tissue.

    abstract::Limited penetration of anticancer drugs through tumour tissue is an important factor that may limit therapeutic effects against solid tumours. Here we report studies of the penetration of radiolabelled methotrexate through multicellular layers (MCL) of murine EMT-6 and human MCF-7 cells grown on semiporous teflon memb...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Cowan DS,Tannock IF

    更新日期:2001-01-01 00:00:00

  • PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.

    abstract::ERK and RSK2 drive proliferation and invasion of many cancers. Phosphoprotein enriched in astrocytes 15 (PEA15) binds ERK and RSK2 and high PEA15 levels can impair ERK- and RSK2-dependent transcription. PEA15 expression also inversely correlates with cell motility and invasiveness. We therefore tested PEA15 effects on...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Gawecka JE,Geerts D,Koster J,Caliva MJ,Sulzmaier FJ,Opoku-Ansah J,Wada RK,Bachmann AS,Ramos JW

    更新日期:2012-10-01 00:00:00

  • Persistence of newly detected human papillomavirus type 31 infection, stratified by variant lineage.

    abstract::Variants of human papillomavirus (HPV) type 31 have been shown to be related both to risk of cervical lesions and racial composition of a population. It is largely undetermined whether variants differ in their likelihood of persistence. Study subjects were women who participated in the ASCUS-LSIL Triage Study and who ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Xi LF,Schiffman M,Koutsky LA,He Z,Winer RL,Hulbert A,Lee SK,Ke Y,Kiviat NB

    更新日期:2013-02-01 00:00:00

  • Adhesion of human breast cancer cell line MCF-7 to human vascular endothelial cells in culture. Enhancement by activated platelets.

    abstract::The interactions of MCF-7 tumor cells with human vascular endothelial cells (EC) and subendothelial extracellular matrices (ECM) were morphologically observed by electron microscopy and quantitatively evaluated by labelling tumor cells with 111Indium-oxine. MCF-7 tumor cells adhered more rapidly to ECM than to the api...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Abecassis J,Millon-Collard R,Klein-Soyer C,Nicora F,Fricker JP,Beretz A,Eber M,Muller D,Cazenave JP

    更新日期:1987-10-15 00:00:00

  • Fluorescence spectroscopy of an in vitro model of human cervical precancer identifies neoplastic phenotype.

    abstract::The clinical diagnosis of cervical neoplasia by spectroscopic methods is potentially a reliable, fast and cost-effective alternative to the conventional smear test. However, it is currently limited by significant inter-patient variation in the spectroscopic properties of the cervix. Characterisation of suitable in vit...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Martin SF,Wood AD,McRobbie MM,Mazilu M,McDonald MP,Samuel ID,Herrington CS

    更新日期:2007-05-01 00:00:00

  • The association of hormonal contraceptive use and HPV prevalence.

    abstract::Women diagnosed with cervical cancer report longer duration and more recent use of combined oral contraceptives (COCs). It is unclear whether COC use is associated with upstream events of human papillomavirus (HPV) infection prior to development of clinical disease. The objective of our study was to assess the associa...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Marks M,Gravitt PE,Gupta SB,Liaw KL,Kim E,Tadesse A,Phongnarisorn C,Wootipoom V,Yuenyao P,Vipupinyo C,Rugpao S,Sriplienchan S,Celentano DD

    更新日期:2011-06-15 00:00:00

  • Survival of cervix cancer patients in Harare, Zimbabwe, 1995-1997.

    abstract::The survival experience of 284 patients with cancer of the cervix uteri registered by the population-based Zimbabwe National Cancer Registry in 1995-1997 is described. The vital status of these subjects was established by linkage with death registration and by retrieval of patient files from medical records department...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Chokunonga E,Ramanakumar AV,Nyakabau AM,Borok MZ,Chirenje ZM,Sankila R,Parkin DM

    更新日期:2004-03-20 00:00:00

  • Recombinant IL-2-activated NK cells mediate LAK activity against ovarian cancer.

    abstract::We investigated the ability of endogenous and recombinant interleukin-2 (IL-2)-stimulated NK cells from normal donors and ovarian cancer patients to mediate lysis of ovarian tumors, and found that peripheral blood (PB) mononuclear cells of normal donors or cancer patients did not display tumoricidal activity against o...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lotzová E,Savary CA,Freedman RS,Edwards CL,Wharton JT

    更新日期:1988-08-15 00:00:00

  • Progression to androgen-independent LNCaP human prostate tumors: cellular and molecular alterations.

    abstract::Lethal phenotypes of human prostate cancer are characterized by progression to androgen-independence and metastasis. For want of a clinically relevant animal model, mechanisms behind this progression remain unclear. Our study used an in vivo model of androgen-sensitive LNCaP human prostate cancer cell xenografts in ma...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Zhou JR,Yu L,Zerbini LF,Libermann TA,Blackburn GL

    更新日期:2004-07-20 00:00:00

  • Iressa induces cytostasis and augments Fas-mediated apoptosis in acinic cell adenocarcinoma overexpressing HER2/neu.

    abstract::Understanding the role of signal transduction in regulating pathways responsible for cell growth, survival and apoptosis is critical for cancer therapy. We developed and characterized a HER2/neu and Fas overexpressing cell line (BNT.888 ACA2) from a salivary gland adenocarcinoma that arose in a HER2/neu transgenic mou...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Piechocki MP,Yoo GH,Dibbley SK,Amjad EH,Lonardo F

    更新日期:2006-07-15 00:00:00

  • A mechanistic study on the metastasis inducing function of FUS-CHOP fusion protein in liposarcoma.

    abstract::The FUS-CHOP fusion protein has been found to be instrumental for specific oncogenic processes in liposarcoma, but its ability to induce metastasis and the underlying mechanisms by which this can be achieved remain unknown. To dissect its functional role in this context, we stably overexpressed this protein in SW872 l...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Patil N,Ahmed Kabeer Rasheed S,Abba M,Hendrik Leupold J,Schwarzbach M,Allgayer H

    更新日期:2014-06-15 00:00:00