Plasma proprotein convertase subtilisin-kexin type 9 does not change during 24h insulin infusion in healthy subjects and type 2 diabetic patients.


PURPOSE:Proprotein convertase subtilisin-kexin type 9 (PCSK9) promotes low density lipoprotein (LDL) receptor degradation, thereby providing a key pathway for LDL metabolism. PCSK9 mRNA expression may be upregulated by insulin in murine models. Here we examined effects of exogenous hyperinsulinemia on plasma PCSK9 levels in humans without and with type 2 diabetes mellitus. METHODS:A 24h moderately hyperinsulinemic glucose clamp (30 mU/kg/h) was performed in 8 healthy men and 8 male type 2 diabetic patients. Plasma PCSK9 was measured using a sandwich enzyme-linked immunosorbent assay. RESULTS:Plasma LDL cholesterol and apolipoprotein B were lowered by insulin in healthy subjects and diabetic patients (P < 0.01 for all), whereas triglycerides were also decreased in healthy subjects (P < 0.01). Plasma PCSK9 levels remained unchanged in healthy subjects (median (interquartile range) change, -23 (-63 to 25) %, P = 0.50) and in diabetic patients (change, 4 (-17 to 44) %, P = 0.20). Individual absolute and relative changes in LDL cholesterol, apolipoprotein B and triglycerides after 24h of insulin were unrelated to changes in PCSK9 (P > 0.15 for all). CONCLUSION:Plasma PCSK9 levels are not increased by exposure to moderate 24h hyperinsulinemia in healthy and type 2 diabetic individuals.






Kappelle PJ,Lambert G,Dullaart RP




Has Abstract


2011-02-01 00:00:00














  • Effect of gemfibrozil on composition of lipoproteins and distribution of LDL subspecies.

    abstract::We studied the effect of gemfibrozil on the compositions of VLDL, LDL and distribution of LDL subspecies in type II hyperlipidemic patients. Gemfibrozil significantly lowered serum triglyceride levels in this group of patients who had normal to moderately elevated triglyceride prior to therapy. Gemfibrozil also change...


    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Tsai MY,Yuan J,Hunninghake DB

    更新日期:1992-07-01 00:00:00

  • The P2Y2 nucleotide receptor is an inhibitor of vascular calcification.

    abstract:BACKGROUND AND AIMS:Mutations in the 5'-nucleotidase ecto (NT5E) gene that encodes CD73, a nucleotidase that converts AMP to adenosine, are linked to arterial calcification. However, the role of purinergic receptor signaling in the pathology of intimal calcification is not well understood. In this study, we examined wh...


    pub_type: 杂志文章


    authors: Qian S,Regan JN,Shelton MT,Hoggatt A,Mohammad KS,Herring PB,Seye CI

    更新日期:2017-02-01 00:00:00

  • A common methylenetetrahydrofolate reductase gene mutation and longevity.

    abstract::Homozygotes (TT genotype) for the C677T mutation in the gene of methylenetetrahydrofolate reductase (C677T/MTHFR mutation) constitute about 12% of the Caucasian population. They have mild hyperhomocysteinemia which is an established risk factor for cardiovascular disease. If the mutation is associated with premature d...


    pub_type: 杂志文章


    authors: Brattström L,Zhang Y,Hurtig M,Refsum H,Ostensson S,Fransson L,Jonés K,Landgren F,Brudin L,Ueland PM

    更新日期:1998-12-01 00:00:00

  • Crocus sativus L. aqueous extract reduces atherogenesis, increases atherosclerotic plaque stability and improves glucose control in diabetic atherosclerotic animals.

    abstract:BACKGROUND AND AIMS:We aimed to evaluate a possible atheroprotective effect of saffron aqueous extract (SFE), and its potential anti-inflammatory mechanisms, in apoE knockout (ApoE-/-) mice. METHODS:Fifty male, ApoE-/- mice, fed a high-fat diet (HFD) for 12 weeks, were randomized into 5 groups: (1) baseline group, eut...


    pub_type: 杂志文章


    authors: Christodoulou E,Kadoglou NPE,Stasinopoulou M,Konstandi OA,Kenoutis C,Kakazanis ZI,Rizakou A,Kostomitsopoulos N,Valsami G

    更新日期:2018-01-01 00:00:00

  • Does cellular sex matter? Dimorphic transcriptional differences between female and male endothelial cells.

    abstract:OBJECTIVE:Significant sex differences exist in cardiovascular diseases. Although an impact of gonadal hormones is presumed, it is largely unknown whether sexually dimorphic gene expression also plays a role and whether cells themselves show intrinsic sex differences. METHODS:We performed whole genome expression analys...


    pub_type: 杂志文章


    authors: Lorenz M,Koschate J,Kaufmann K,Kreye C,Mertens M,Kuebler WM,Baumann G,Gossing G,Marki A,Zakrzewicz A,Miéville C,Benn A,Horbelt D,Wratil PR,Stangl K,Stangl V

    更新日期:2015-05-01 00:00:00

  • Associations between retinal microvascular structure and the severity and extent of coronary artery disease.

    abstract:OBJECTIVE:Microvascular mechanisms are increasingly recognized as being involved in a significant proportion of coronary artery disease (CAD) cases, but their exact contribution or role is unclear. We aimed to define the association between retinal microvascular signs and both CAD extent and severity. METHODS:1120 par...


    pub_type: 杂志文章


    authors: Gopinath B,Chiha J,Plant AJ,Thiagalingam A,Burlutsky G,Kovoor P,Liew G,Mitchell P

    更新日期:2014-09-01 00:00:00

  • Total cholesterol and stroke mortality in middle-aged and elderly adults: A prospective cohort study.

    abstract:BACKGROUND AND AIMS:The association between cholesterol and stroke has been inconsistent. This study aimed to examine the association between total cholesterol (TC) and mortality from total stroke and stroke subtypes. METHODS:503,340 Korean adults aged 40-80 years without a history of heart disease or stroke participa...


    pub_type: 杂志文章


    authors: Yi SW,Shin DH,Kim H,Yi JJ,Ohrr H

    更新日期:2018-03-01 00:00:00

  • A monocyte chemoattractant protein-1 gene polymorphism is associated with occult ischemia in a high-risk asymptomatic population.

    abstract::Monocyte chemoattractant protein-1 (MCP-1) recruits monocytes into atherosclerotic plaques. A single nucleotide polymorphism in the MCP-1 gene promoter (-2578A>G) results in greater production of MCP-1 protein. We examined the association of this polymorphism with occult coronary artery disease (CAD) and its interacti...


    pub_type: 临床试验,杂志文章


    authors: Kim MP,Wahl LM,Yanek LR,Becker DM,Becker LC

    更新日期:2007-08-01 00:00:00

  • One-year treatment with exenatide vs. insulin glargine: effects on postprandial glycemia, lipid profiles, and oxidative stress.

    abstract:OBJECTIVE:The objective of the present study was to investigate the effects of one-year treatment with exenatide or Insulin Glargine, followed by a 5-week off-drug period, on postprandial lipidaemia, glycaemia and measures of oxidative stress. METHODS:Sixty-nine metformin-treated patients with type 2 diabetes were ran...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Bunck MC,Cornér A,Eliasson B,Heine RJ,Shaginian RM,Wu Y,Yan P,Smith U,Yki-Järvinen H,Diamant M,Taskinen MR

    更新日期:2010-09-01 00:00:00

  • Low dose apolipoprotein A-I rescues carotid arteries from inflammation in vivo.

    abstract::This study investigates the ability of a single, low dose of apolipoprotein (apo) A-I, the main lipoprotein of high density lipoproteins (HDL), to inhibit acute vascular inflammation in normocholesterolemic New Zealand White rabbits. Acute vascular inflammation was induced in the animals by placing a non-occlusive, si...


    pub_type: 杂志文章


    authors: Puranik R,Bao S,Nobecourt E,Nicholls SJ,Dusting GJ,Barter PJ,Celermajer DS,Rye KA

    更新日期:2008-01-01 00:00:00

  • Adiposity has no direct effect on carotid intima-media thickness in adolescents and young adults: Use of structural equation modeling to elucidate indirect & direct pathways.

    abstract:BACKGROUND:Carotid intima-media thickness (cIMT) is associated with CV events in adults. Thicker cIMT is found in youth with CV risk factors including obesity. Which risk factors have the most effect upon cIMT in youth and whether obesity has direct or indirect effects is not known. We used structural equation modeling...


    pub_type: 杂志文章


    authors: Gao Z,Khoury PR,McCoy CE,Shah AS,Kimball TR,Dolan LM,Urbina EM

    更新日期:2016-03-01 00:00:00

  • Effects of magnesium oxide on the lipid profile of healthy volunteers.

    abstract::Elevated serum cholesterol is a risk factor in the development of coronary artery disease. Magnesium has been reported to decrease total serum cholesterol, low density lipoprotein, and very low density lipoprotein, and increase high density lipoprotein. A randomized, double-blind, placebo-controlled, crossover study w...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Marken PA,Weart CW,Carson DS,Gums JG,Lopes-Virella MF

    更新日期:1989-05-01 00:00:00

  • Serum total and high density lipoprotein (HDL) cholesterol concentrations in rural and urban boys from 16 countries.

    abstract::Serum total and HDL cholesterol concentrations were measured in schoolboy populations from 16 countries. Total and HDL cholesterol concentrations were determined in one laboratory to reduce methodological variability as much as possible. A standardized protocol was used for the drawing of blood, preparation, storage a...


    pub_type: 杂志文章


    authors: Knuiman JT,Hermus RJ,Hautvast JG

    更新日期:1980-08-01 00:00:00

  • Complement proteins and arterial calcification in middle aged women: Cross-sectional effect of cardiovascular fat. The SWAN Cardiovascular Fat Ancillary Study.

    abstract:BACKGROUND:CVD risk increases in women after menopause. Recent data suggest higher levels of complement protein C3 and cardiovascular fat (CF) in postmenopausal women. Whether complement proteins are associated with early markers of atherosclerosis in healthy midlife women has never been evaluated. Additionally, the po...


    pub_type: 杂志文章,多中心研究


    authors: Nagaraj N,Matthews KA,Shields KJ,Barinas-Mitchell E,Budoff MJ,El Khoudary SR

    更新日期:2015-12-01 00:00:00

  • Endothelial dysfunction in hypercholesterolemia is improved by L-arginine administration: possible role of oxidative stress.

    abstract::Hypercholesterolemia impairs endothelial function. However, the production/release of nitric oxide from the hypercholesterolemic aorta is reported to be enhanced rather than impaired in animal studies. L-arginine improves endothelial function in hypercholesterolemic subjects. The goal of the present study was to inves...


    pub_type: 临床试验,杂志文章


    authors: Kawano H,Motoyama T,Hirai N,Kugiyama K,Yasue H,Ogawa H

    更新日期:2002-04-01 00:00:00

  • Alterations in composition and concentration of lipoproteins and elevated cholesteryl ester transfer in non-insulin-dependent diabetes mellitus (NIDDM).

    abstract::Cholesteryl esters (CE) exchange between lipoproteins through the action of cholesteryl ester transfer protein (CETP). Situations at high risk for atherosclerosis are often accompanied by an accelerated net mass CE transfer (CET) from high density lipoproteins (HDL) to very low (VLDL) and low density lipoproteins (LDL...


    pub_type: 杂志文章


    authors: Elchebly M,Porokhov B,Pulcini T,Berthezène F,Ponsin G

    更新日期:1996-06-01 00:00:00

  • The state of macrophage differentiation determines the TNF alpha response to nitrated lipoprotein uptake.

    abstract::Inflammatory cytokine synthesis by monocyte-macrophages in the developing plaque represents an important amplification point in atherosclerotic disease progression. Here we have investigated whether the state of monocyte-macrophage differentiation can influence TNF alpha synthesis in response to scavenged modified low...


    pub_type: 杂志文章


    authors: Smythe CD,Skinner VO,Bruckdorfer KR,Haskard DO,Landis RC

    更新日期:2003-10-01 00:00:00

  • Diabetic state as a crucial factor for impaired arterial elastic properties in patients with peripheral arterial disease.

    abstract:BACKGROUND:Increased arterial stiffness is associated with greater risk of cardiovascular events and mortality. However, in patients with peripheral arterial disease (PAD) who have severe atherosclerotic disorder or risk clustering, the major determinants of increased stiffness have been not clarified. METHODS:This st...


    pub_type: 杂志文章


    authors: Makita S,Matsui H,Naganuma Y,Abiko A,Tamada M,Nakamura M

    更新日期:2010-01-01 00:00:00

  • Contribution of apolipoprotein(a) size, pentanucleotide TTTTA repeat and C/T(+93) polymorphisms of the apo(a) gene to regulation of lipoprotein(a) plasma levels in a population of young European Caucasians.

    abstract::Several studies indicate that the inter-individual variation in plasma concentrations of lipoprotein(a) (Lp(a)) is mainly under genetic control. To define the effect of three DNA polymorphisms on apolipoprotein(a) (apo(a)) expression, we have determined plasma Lp(a) concentrations, apo(a) isoform size, KpnI allele siz...


    pub_type: 杂志文章


    authors: Valenti K,Aveynier E,Leauté S,Laporte F,Hadjian AJ

    更新日期:1999-11-01 00:00:00

  • Tissue lipoprotein lipase in the hyperthyroid rat. Effect of growth and aging.

    abstract::The hydrolysis of lipoprotein triglycerides is catalyzed by lipoprotein lipase (LPL), an enzyme found in many tissues. We have examined tissue LPL activity (LPLA) in rats with experimentally-induced hyperthyroidism. In younger, lighter rats, hyperthyroidism was accompanied by a decrease in LPL in adipose tissue wherea...


    pub_type: 杂志文章


    authors: Wilson DE,Jubiz W,Flowers CM,Carlile SI,Adolf AM

    更新日期:1977-03-01 00:00:00

  • Cost-effectiveness of pravastatin in secondary prevention of coronary heart disease: comparison between Belgium and the United States of a projected risk model.

    abstract::Methodological differences and variations in health care regulations among countries often preclude direct comparisons of cost-effectiveness studies. A projected risk model was applied, designed to determine the economic value in the United States of pravastatin in the secondary prevention of coronary heart disease (C...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Muls E,Van Ganse E,Closon MC

    更新日期:1998-04-01 00:00:00

  • Sex-related differences in Japanese patients with peripheral arterial disease.

    abstract:OBJECTIVE:The goal of the study was to examine possible sex-related differences in the clinical characteristics and risk factors in Japanese patients with peripheral arterial disease (PAD). METHODS:Sex-related differences in clinical profiles, risk factors and treatments were examined in 730 consecutive patients with ...


    pub_type: 杂志文章


    authors: Kumakura H,Kanai H,Araki Y,Kasama S,Sumino H,Ito T,Iwasaki T,Takayama Y,Ichikawa S,Fujita K,Nakashima K,Minami K

    更新日期:2011-12-01 00:00:00

  • 18Fluoride-based molecular imaging of coronary atherosclerosis in HIV infected patients.

    abstract:BACKGROUND AND AIMS:Molecular imaging with 18Fluorodeoxyglucose (FDG) and 18F-sodium-fluoride (NaF) captures arterial inflammation and micro-calcification and can reveal potentially unstable atherosclerotic plaques. METHODS:We performed FDG and NaF PET/CT imaging in two clinically similar cohorts of patients living wi...


    pub_type: 杂志文章


    authors: Guaraldi G,Milic J,Prandini N,Ligabue G,Esposito F,Ciusa G,Malagoli A,Scaglioni R,Besutti G,Beghetto B,Nardini G,Roncaglia E,Mussini C,Raggi P

    更新日期:2020-03-01 00:00:00

  • Associations of plasma homocysteine and the methylenetetrahydrofolate reductase C677T polymorphism with carotid intima media thickness among South Asian, Chinese and European Canadians.

    abstract:BACKGROUND:Few studies have evaluated the associations of plasma homocysteine concentration, the methylenetetrahydrofolate reductase (MTHFR) C677T genotype and B vitamin concentration with intima media thickness (IMT) in multiethnic populations. METHODS:In the Study of Health Assessment and Risk in Ethnic groups (SHAR...


    pub_type: 杂志文章


    authors: Kelemen LE,Anand SS,Hegele RA,Stampfer MJ,Rosner B,Willett WC,Montague PA,Lonn E,Vuksan V,Teo KK,Devanesen S,Yusuf S

    更新日期:2004-10-01 00:00:00

  • The SH2B1 obesity locus is associated with myocardial infarction in diabetic patients and with NO synthase activity in endothelial cells.

    abstract:OBJECTIVE:Obesity and cardiovascular disease recognize a common metabolic soil and may therefore share part of their genetic background. Genome-wide association studies have identified variability at the SH2B1 locus as a predictor of obesity. We investigated whether SNP rs4788102, which captures the entire SH2B1 variab...


    pub_type: 杂志文章,多中心研究


    authors: Prudente S,Morini E,Larmon J,Andreozzi F,Di Pietro N,Nigro A,Gervino EV,Mannino GC,Bacci S,Hauser TH,Bellacchio E,Formoso G,Pellegrini F,Proto V,Menzaghi C,Frittitta L,Pandolfi A,Sesti G,Doria A,Trischitta V

    更新日期:2011-12-01 00:00:00

  • Latvian registry of familial hypercholesterolemia: The first report of three-year results.

    abstract:BACKGROUND AND AIMS:Familial hypercholesterolemia (FH) was rarely diagnosed in Latvia before 2015, when the Latvian Registry of FH (LRFH) was established. Here, we report the first experience of the LRFH over three years (2015-2017). METHODS:The LRFH is an ongoing nationwide, dynamic, long-term prospective cohort. The...


    pub_type: 杂志文章


    authors: Latkovskis G,Saripo V,Gilis D,Nesterovics G,Upena-Roze A,Erglis A

    更新日期:2018-10-01 00:00:00

  • Coronary artery endothelial dysfunction is positively correlated with low density lipoprotein and inversely correlated with high density lipoprotein subclass particles measured by nuclear magnetic resonance spectroscopy.

    abstract:OBJECTIVE:The association between cholesterol and endothelial dysfunction remains controversial. We tested the hypothesis that lipoprotein subclasses are associated with coronary endothelial dysfunction. METHODS AND RESULTS:Coronary endothelial function was assessed in 490 patients between November 1993 and February 2...


    pub_type: 杂志文章


    authors: Ford MA,McConnell JP,Lavi S,Rihal CS,Prasad A,Sandhu GS,Hartman SJ,Lerman LO,Lerman A

    更新日期:2009-11-01 00:00:00

  • The effect of chlorthalidone on serum lipids and lipoproteins.

    abstract::The effect of chlorthalidone treatment on serum lipids and lipoproteins was investigated in 21 hypertensive patients. Chlorthalidone caused an increase in total serum and low density lipoprotein (LDL) cholesterol, but not in high density lipoprotein (HDL) cholesterol. Serum and lipoprotein triglycerides also increased...


    pub_type: 杂志文章


    authors: Rosenthal T,Holtzman E,Segal P

    更新日期:1980-05-01 00:00:00

  • The effect of hormone replacement therapy on arterial distensibility and compliance in perimenopausal women: a 2-year randomised trial.

    abstract::A single centre randomised placebo-controlled trial was performed to assess the 2-year effects of hormone replacement therapy compared to placebo on mechanical arterial properties in 99 perimenopausal women recruited from the general population. The trial was double-blind with respect to a sequential combined regimen ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Westendorp IC,de Kleijn MJ,Bots ML,Bak AA,Planellas J,Coelingh Bennink HJ,Hofman A,Grobbee DE,Witteman JC

    更新日期:2000-09-01 00:00:00

  • Novel genetic variants linked to coronary artery disease by genome-wide association are not associated with carotid artery intima-media thickness or intermediate risk phenotypes.

    abstract:BACKGROUND:It is uncertain whether the novel single nucleotide polymorphisms (SNPs) that have recently been associated with coronary artery disease (CAD) in genome-wide studies also influence carotid atheroma and stroke risk. The mechanisms of their association with CAD are unknown; relationships to other cardiovascula...


    pub_type: 信件


    authors: Cunnington MS,Mayosi BM,Hall DH,Avery PJ,Farrall M,Vickers MA,Watkins H,Keavney B

    更新日期:2009-03-01 00:00:00