Abstract:
BACKGROUND & AIMS:Rabs are monomeric guanosine triphosphatases that regulate membrane trafficking and acid secretion in gastric parietal cells. Using a proteomics approach, we identified a new Rab, Rab27b, in tubulovesicle membranes and determined its role in parietal cell activation. METHODS:We used mass spectrometry (MS) to identify Rab27b in purified tubulovesicular membrane fractions and used immunoblot and immunofluorescence analyses to study its expression. Wild-type, constitutively active (Rab27bQ78L), and dominant negative (Rab27bN133I) forms of Rab27b were tagged with yellow fluorescent protein (YFP) and expressed in parietal cells using adenoviral constructs to study localization and function. Localization was visualized by fluorescence microscopy in resting and stimulated cells. Acid secretion in primary cell cultures was measured by aminopyrine accumulation. RESULTS:A tandem MS peptide mass fingerprint was matched to 7 peptides of Rab27b. Rab27b localized to tubulovesicle membranes, based on immunoblot and immunocytochemical analyses. Endogenous Rab27b, YFP/wild-type Rab27b, Rab27bQ78L, and Rab27bN133I all distributed throughout the cytoplasm of resting parietal cells. After stimulation, wild-type Rab27b and YFP-Rab27bQ78L translocated to the apical membrane, but YFPR-ab27bN133I did not. Expression of wild-type YFP-Rab27b or YFP-Rab27bQ78L did not affect acid secretion, whereas expression of Rab27bN133I almost completely inhibited acid secretion. CONCLUSIONS:Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Suda J,Zhu L,Okamoto CT,Karvar Sdoi
10.1053/j.gastro.2010.09.044subject
Has Abstractpub_date
2011-03-01 00:00:00pages
868-78issue
3eissn
0016-5085issn
1528-0012pii
S0016-5085(10)01453-8journal_volume
140pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND/AIMS:Platelets show proinflammatory as well as prothrombotic properties. Patients with inflammatory bowel disease are at increased risk of systemic thromboembolism, and multifocal microvascular infarction has been proposed as a pathogenetic mechanism in Crohn's disease. The aim of this study was to determine...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(94)90741-2
更新日期:1994-04-01 00:00:00
abstract::It has been suggested that the lower esophageal sphincter (LES) plays an important role in preventing gastroesophageal reflux. This study was designed to determine if abolition of the LES alone is sufficient to induce gastroesophageal reflux and esophagitis. Studies were performed in the Australian brush-tailed possum...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1975-09-01 00:00:00
abstract::Previous investigations demonstrated that S-adenosyl-L-methionine suppresses the cholestatic effect of ethynylestradiol and reduces the irreversible binding of the estrogen to rat liver microsomes, probably favoring ethynylestradiol transformation into its methyl-derivatives. Since it is not known whether SAMe also pr...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract:BACKGROUND & AIMS:Hepcidin is an antimicrobial peptide thought to be involved in the regulation of intestinal iron absorption. To further investigate its role in this process, we examined hepatic and duodenal gene expression in rats after the switch from a control diet to an iron-deficient diet. METHODS:Adult rats on ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2002.35353
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND & AIMS:The ratio of liver size to body weight (hepatostat) is tightly controlled, but little is known about how the physiologic functions of the liver help determine its size. Livers of mice repopulated with human hepatocytes (humanized livers) grow to larger than normal; the human hepatocytes do not recogni...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2015.05.043
更新日期:2015-09-01 00:00:00
abstract::For decades, interactions between the enteric neuromuscular apparatus and the central nervous system have served as the primary focus of pathophysiological research in the functional gastrointestinal disorders. The accumulation of patient reports, as well as clinical observations, has belatedly led to an interest in t...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2016.02.028
更新日期:2016-02-18 00:00:00
abstract:BACKGROUND & AIMS:The revised Bethesda guidelines for Lynch syndrome recommend microsatellite instability (MSI) testing all colorectal cancers in patients diagnosed before age 50 years and colorectal cancers diagnosed in patients between ages 50 and 59 years with particular pathology features. Our aim was to identify p...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.04.044
更新日期:2007-07-01 00:00:00
abstract::Flow cytometry was used to detect the presence and assess the distribution of aneuploid cell populations in eight proctocolectomy specimens from patients with ulcerative colitis. Mucosal samples were taken according to a systematic protocol for flow cytometry, the surrounding tissue was examined histologically, and th...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(91)90068-v
更新日期:1991-11-01 00:00:00
abstract:BACKGROUND & AIMS:Major histocompatibility complex (MHC) class I-restricted CD8(+) T cells are required for clearance of hepatitis C virus (HCV) infection. MHC class I expression is up-regulated by type I and II interferons (IFNs). However, little is known about the effects of HCV infection on IFN-induced expression of...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2014.01.054
更新日期:2014-05-01 00:00:00
abstract::In rats fasted 4 days, immunoreactive gastrin concentrations decreased to one-third of the fed levels in antral tissue and to one-eight of the fed levels in serum. The number of antral cells that reacted with fluorescent antigastrin antiserum was also correspondingly decreased. After refeeding, serum gastrin returned ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1975-06-01 00:00:00
abstract::Colchicine can induce the malabsorption of vitamin B12 and other nutrients. Previous investigations have suggested but not proved that this malabsorption was due to a lesion in the ileal mucosa. Employing the receptor assay of M. Katz and B. A. Cooper (J Clin Invest 54:733-739, 1974), the authors have observed a dose-...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
abstract::Peripheral blood leukocyte counts, percentages of lymphocytes and granulocytes, serum albumin levels, and results of skin tests with five antigens were examined in patients randomized in the National Cooperative Crohn's Disease Study. Inspection for differences from normal and correlations with a variety of variables ...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1979-10-01 00:00:00
abstract::The prevalence of gastroesophageal varices and gastrointestinal hemorrhage was determined in 124 patients with severe chronic active liver disease (CALD) receiving prednisone and followed regularly for up to 10 yr. Varices were demonstrated by contrast radiography in only 19 patients (15%). Ten had varices before ther...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1979-10-01 00:00:00
abstract::It is generally accepted that the intestinal phase of pancreatic secretion is initiated by the stimulation of chemoreceptors sensitive to fat and protein degradation products and hydrogen ions. The effect of the volume and osmolality of food emptied by the stomach into the duodenum has received less attention. We inve...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND:Fiber and water-holding agents are used for the treatment of constipation. In what may appear to be a paradox, they are sometimes also used for the treatment of diarrhea; it has been proposed that they sequester water from liquid stools and/or increase the ratio of fecal solids to fecal water and thereby imp...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0016-5085(93)90267-g
更新日期:1993-04-01 00:00:00
abstract::Numerous epidemiologic studies suggest a relationship between lung cancer and peptic ulcer disease. Furthermore, various lung cancers synthesize and release a number of peptides such as gastrin and gastrin-releasing peptide that could cause acid hypersecretion; however, Zollinger-Ellison syndrome (ZES), because of a l...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2001.23242
更新日期:2001-04-01 00:00:00
abstract::An animal model for giardiasis was developed using Giardia muris in Swiss albino mice. Intraesophageal inoculation of G. muris cysts caused a reproducible pattern of infection, with trophozoite and cyst counts reaching a maximum on days 5 to 14 after cyst inoculation and thereafter showing a progressive decline. Spont...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1976-07-01 00:00:00
abstract::The hepatic circulation is reviewed with emphasis on the role of hepatic blood vessels in hepatic and homeostatic functions. Contrasts are made with resistance, capacitance, and fluid exchange functions in other better known vascular beds. Hemodynamic changes that produce shifts in fluid exchange in other tissues are ...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:
更新日期:1977-11-01 00:00:00
abstract::Rising trends in the incidence and mortality of early-onset CRC in those who are ages less than 50 years have been well-established. These trends have spurred intense investigation focused on elucidating the epidemiology and characteristics of early-onset CRC, as well on identifying strategies for early detection and ...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2020.12.068
更新日期:2021-01-05 00:00:00
abstract:BACKGROUND & AIMS:Three-dimensional (3D) ultrasound imaging of the total stomach volume has not yet been achieved. The aim of this study was to investigate whether a magnetic position sensor system for acquisition of 3D ultrasonograms could be used to determine gastric emptying rates and intragastric distribution. MET...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(97)70078-7
更新日期:1997-07-01 00:00:00
abstract:BACKGROUND & AIMS:Cyclosporin A specifically suppresses hepatitis C virus (HCV) replication in vitro at clinically achievable concentrations. In this study, we investigated the mechanisms of action of cyclosporin A against HCV replication. METHODS:The in vitro effects of cyclosporin A on HCV replication were analyzed ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2005.06.031
更新日期:2005-09-01 00:00:00
abstract::Serial studies of plasma lipids and lipoproteins were performed in 4 patients with acute alcoholic liver disease characterized by a massive fatty liver and laboratory evidence of intrahepatic cholestasis. There were striking alterations in the plasma lipoprotein electrophoretic patterns characterized by the absence of...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::Increasing evidence has emerged that supports an important intersection between 3 fundamental cell biologic pathways in the pathogenesis of inflammatory bowel disease. These include the intersection between autophagy, as revealed by the original identification of ATG16L1 and IRGM as major genetic risk factors for Croh...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2011.02.048
更新日期:2011-05-01 00:00:00
abstract:BACKGROUND & AIMS:Changes in the properties of visceral sensory neurons contribute to the development of gastrointestinal pain. However, little is known about the molecules involved in mechanosensation from the gastrointestinal tract. We investigated the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2008.01.031
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND & AIMS:Crohn's disease is a highly heterogeneous inflammatory bowel disease comprising multiple clinical phenotypes. Genome-wide association studies (GWASs) have associated a large number of loci with disease risk but have not associated any specific genetic variants with clinical phenotypes. We performed a ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2014.12.030
更新日期:2015-04-01 00:00:00
abstract::Hepatic plasma flow was assessed with sorbitol (hepatic extraction = 0.96) at steady state. After infusion of 50 mg/min for 3 h, total and renal sorbitol clearances were calculated, and the extrarenal clearance was obtained by taking the difference between the two. In normal volunteers, the mean (+/- SD) extrarenal so...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(88)80024-6
更新日期:1988-09-01 00:00:00
abstract:BACKGROUND & AIMS:This study investigated whether obesity, diabetes, and other metabolic factors are independently associated with hepatocellular carcinoma (HCC), stratified by hepatitis B virus (HBV) and hepatitis C virus (HCV) serostatus, and explored the possible joint influence of obesity/diabetes and HBV/HCV infec...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2008.03.073
更新日期:2008-07-01 00:00:00
abstract::Increased type III collagen deposition in all layers of the intestinal tract, including the lamina propria, is a common feature of strictures in Crohn's disease. In the present study, it was found that in comparison with fibroblasts from normal or nonstrictured but inflamed intestinal lamina propria, the fibroblasts i...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)90314-o
更新日期:1992-06-01 00:00:00
abstract::We have studied the effect of short-term high-dose prednisone therapy on aminotransferase levels and hepatitis B virus markers in 6 patients with chronic type B hepatitis. All showed a decrease in amino transferase levels during treatment. This was followed by a transient increase in aminotransferase levels after pred...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1983-05-01 00:00:00
abstract::The effect of substance P (SP) on the release of gastric somatostatinlike immunoreactivity (SLI) was studied. Substance P inhibited both basal SLI release and SLI release stimulated by gastric inhibitory polypeptide or 1-isoproterenol. This inhibitory action of SP was not blocked by atropine or hexamethonium, suggesti...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(85)80138-4
更新日期:1985-01-01 00:00:00