Abstract:
:In astrocytes, ion currents are predominantly carried by K(+) ions, and their potassium channel expression changes during development. Here, we studied ion current generated by voltage-ramp protocols in cultured astrocytes from the corpus callosum of newborn (P0) and 28-day-old (P28) rats. Inward currents measured at -140 mV and chord conductances measured from -140 to -75 mV, were smaller in P0-astrocytes than in P28-astrocytes, and in P28-astrocytes were affected by 100 μM Ba(2+), indicating the presence of an inward rectifier K(+) (Kir) current. On the other hand, P0-astrocytes showed higher outward current measured at 80 mV and a higher chord conductance, between 0 and 80 mV, than P28-astrocytes. The outward current was more potently reduced by 2mM Ba(2+) in P0-astrocytes than in P28-astrocytes, and slightly reduced at both ages using low concentrations of Ba(2+). Moreover, outward current was partially blocked by iberiotoxin in P0-astrocytes, indicating the presence of big-conductance Ca(2+)-activated K(+) (BK) channels. In addition, 4-aminopyridine inhibited the outward current in P0- and P28-astrocytes. In summary, P0-astrocytes exhibited the BK current, a major density of delayed rectifier K(+) (K(DR)) current, and a low density of the Kir current, whereas P28-astrocytes presented a major density of Kir current, a low density of the K(DR) current, and the absence of BK current. These results could contribute to a better understanding of the role of K(+) currents in the corpus callosum.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Montiel-Herrera M,García-Colunga Jdoi
10.1016/j.neulet.2010.09.009subject
Has Abstractpub_date
2010-11-26 00:00:00pages
189-93issue
3eissn
0304-3940issn
1872-7972pii
S0304-3940(10)01200-0journal_volume
485pub_type
杂志文章abstract::To differentiate the opiate (naloxone) receptor and the enkephalin receptor in rat brain, we solubilized the receptor molecules by detergent and determined the molecular weights by gel filtration. The receptor preparation was bound to [3H] naloxone or [3H] Met5-enkephalin, and was solubilized by Triton X-100. On gel c...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(81)90269-x
更新日期:1981-12-11 00:00:00
abstract::Optic fibre regeneration in young Xenopus was studied by [(3)H]proline autoradiography and by mapping the visuotectal projection electrophysiologically from 5 to 210 days after sectioning the right optic nerve. Regenerated optic fibers were demonstrated morphologically both in the contra and ipsilateral tecta. In cont...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(78)90114-3
更新日期:1978-01-01 00:00:00
abstract::The acute thermoregulatory effect of microinjection of capsaicin into the nucleus raphe dorsalis (NRD) has been studied in anesthetised rats. Application of capsaicin (20 micrograms) into the NRD increased the tail skin temperature and decreased the body temperature. In animals that had previously been treated with ca...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(87)90297-7
更新日期:1987-03-31 00:00:00
abstract::A calcium-dependent potassium channel K(Ca) has been isolated in mouse motoneurons. With physiological concentrations of potassium across inside-out patches, a 100 pS K(Ca) channel was activated when the bath solution of Ca2+ was in excess of 1 microM. Introduction of the drug tedisamil, a blocker of repolarizing pota...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90746-t
更新日期:1992-09-14 00:00:00
abstract::Nerve fibers immunoreactive for vasoactive intestinal polypeptide (VIP) were demonstrated by immunocytochemistry in the dog and rat kidney. They were seen in association with the renal artery and its branches. In the dog, VIP-immunoreactive fibers were rarely seen close to small blood vessels suggestive of arterioles....
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(83)90199-4
更新日期:1983-12-30 00:00:00
abstract::Depression associated with diabetes has been described as a highly debilitating comorbidity. Due to its complex and multifactorial mechanisms, the treatment of depression associated with diabetes represents a clinical challenge. Cannabidiol (CBD), the non-psychotomimetic compound derived from Cannabis sativa, has been...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2018.06.006
更新日期:2018-08-24 00:00:00
abstract::Adult rat chromaffin cells proliferate in vivo in response to neurally derived signals. Their proliferation in vitro is stimulated either by peptide growth factors or by activators of adenylate cyclase or protein kinase C that mimic the effects of neurotransmitters in adrenal medullary nerve endings. Differing suscept...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90445-6
更新日期:1994-02-28 00:00:00
abstract::The fine structure of striato-nigral efferent neurons, retrogradely labeled with HRP, was studied. The neuron showed a medium sized ovoid or tri-angular perikaryon. Short or medium lengthened granular endoplasmic reticula, having a tendency to wind and pile up in stacks were observed. This neurons corresponds to a typ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(79)95335-7
更新日期:1979-09-01 00:00:00
abstract::The content of two neuropeptides, somatostatin (SRIF) and neuropeptide Y (NPY) has been determined in two cerebral cortical areas of Alzheimer's disease brain and in age-matched control brains. The content of SRIF-like immunoreactivity (SRIF-LI) was found to be decreased in Alzheimer temporal cortex (Brodmann area 21)...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90455-6
更新日期:1986-09-25 00:00:00
abstract::In the rat, unilateral dorsal cervicothoracic rhizotomy (C5-T2) resulted in autotomy of the ipsilateral limb. The onset of self-mutilation was variable and attained the maximum degree 8-9 weeks after the dorsal root section. Fifteen and 60 days after the lesion the monoamines dopamine (DA), noradrenaline (NA) and sero...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90466-1
更新日期:1988-05-03 00:00:00
abstract::The study investigated the effects of metabolic syndrome on urinary bladder nerve-growth factor (NGF) and p75(NTR) expression in fructose-fed obese rats. Age-matched male Wistar rats were divided into four groups; group I: normal control rats; group II: 6-week fructose-fed rats (FR); group III: 9-week FR; and group IV...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.06.008
更新日期:2008-08-15 00:00:00
abstract::Extracellular field-potential recordings and measurements of the extracellular concentration of potassium ([K+]o) were made in layers II and III of the adult rat entorhinal cortex in a slice preparation. Two types of spontaneous, synchronous potentials were induced by the convulsant drug 4-aminopyridine (4AP, 50 micro...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)11248-u
更新日期:1995-02-13 00:00:00
abstract::The intracerebroventricular injection of the mast cell degranulator, compound 48/80 (C48/80, 10 micrograms/kg), produced a marked behavioural syndrome in rats which included head and body shakes, paw tremor, excessive grooming, unusual posture and gait, mild diarrhoea, piloerection, extreme agitation and irritability ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90124-2
更新日期:1986-03-28 00:00:00
abstract::The underlying mechanism of isoflurane-induced cognitive dysfunction in older individuals is unknown. In this study, the effects of isoflurane exposure on the hippocampal blood-brain barrier (BBB) in aged rats were investigated because it was previously shown that BBB disruption involves in cognitive dysfunction. Twen...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2014.12.018
更新日期:2015-02-05 00:00:00
abstract::Ethanol consumption affects levels of endogenous opioids as well as opioid receptors in both animals and humans. We studied the expression of delta (delta) and mu (mu) opioid receptors (ORs) in brain sections of adult male Sprague-Dawley rats after 2 weeks of consuming ethanol in a liquid diet, with comparisons to sec...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.02.016
更新日期:2005-06-10 00:00:00
abstract::The present magnetoencephalographic study was conducted in order to assess the accuracy of dipole localizations for the movement-evoked field component 1 (MEF 1). Three male subjects were requested to perform self-paced flexions of their index finger and thumb in repeated sessions of 60 trials while the neuromagnetic ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(96)12952-9
更新日期:1996-08-30 00:00:00
abstract::Molecular forms of butyrylcholinesterase (BuChE) were studied in microvessels isolated from rat brain, using sedimentation analysis in sucrose gradients. Three forms, G1, G2 and G4, were found with sedimentation coefficients close to 3S, 6S and 9S, respectively. The relative proportion of the 3 forms was 19% for the m...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90164-5
更新日期:1990-11-27 00:00:00
abstract::We tested the hypothesis that intracerebroventricular (i.c.v.) administration of leptin would increase mean arterial pressure (MAP) in ad libitum (AL) fed and food deprived (FD) normotensive rats. Male Sprague-Dawley rats were chronically instrumented with a guide cannula directed at the lateral ventricle and a caroti...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(98)00223-7
更新日期:1998-04-17 00:00:00
abstract::In anesthetized cats selective activation of fine muscle afferents of the hindlimb by KCl or bradykinin (Brad.) induced reflex changes in the urinary bladder. The nature of the reflex responses depended on the functional state of the bladder: they were excitatory when the bladder was quiescent and inhibitory during la...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(79)90048-x
更新日期:1979-01-01 00:00:00
abstract::In rats with hyperalgesia of the obliquus externus muscle (OE) from artificial calculosis of the ipsilateral upper ureter, changes in cell activity were studied in the ipsilateral spinal cord (T11-T12) versus control animals. In cases of hyperalgesia of high degree, in the dorsal horn (0-900 microns) the following wer...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)12267-2
更新日期:1996-01-19 00:00:00
abstract::Which cognitive processes are reflected by the N400 in ERPs is still controversial. Various recent articles (Lau et al., 2008; Brouwer et al., 2012) have revived the idea that only lexical pre-activation processes (such as automatic spreading activation, ASA) are strongly supported, while post-lexical integrative proc...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2017.05.007
更新日期:2017-06-09 00:00:00
abstract::The degree of contamination by blood in macroscopically clear cerebrospinal fluid (CSF) from the rat was assessed by the red blood cell count. Amino acid concentrations in the same samples were determined using high performance liquid chromatography. A significant positive correlation between the number of erythrocyte...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90606-3
更新日期:1986-08-29 00:00:00
abstract::In gastropods, the interneuronal messenger, nitric oxide (NO), modulates spike frequency and synaptic transmission. We have characterized the effect of NO on ion currents underlying neuronal excitability, using current-clamp and two-electrode voltage-clamp techniques. Identified neurons of the pulmonate snail, Helix p...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(00)01606-2
更新日期:2000-12-08 00:00:00
abstract::The preprocholecystokinin gene is expressed in a subpopulation of cortical interneurons containing gamma-aminobutyric acid. Slices of neonatal rat cortex were cultivated for 12 +/- 2 days and examined for the presence and distribution of these neurons by in situ hybridization and immunocytochemistry. Like in situ, two...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90708-s
更新日期:1993-06-11 00:00:00
abstract::Activation of several immediate early genes (IEGs) is crucial for long-term memory formation in vivo. In vitro methods of inducing these genes have not been investigated extensively. Here we present data demonstrating that application of the neurotrophin, nerve growth factor (NGF), to both rat primary neuronal culture...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.04.089
更新日期:2004-08-05 00:00:00
abstract::A previous study of brain activation in normal subjects during a phonological (Phonemes) task and a lexical semantic (Words) task using positron-emission tomography [3] is complemented by new data after a method for image realignment was applied [17]. The pattern of cerebral blood flow increases associated with Words ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90196-1
更新日期:1994-11-21 00:00:00
abstract::RE-1 silencer transcription factor (REST), also known as neuron-restrictive silencer factor (NRSF), contains nine Cys2-His2 type zinc finger domains (ZFDs). REST/NRSF is localized to the nucleus, where it represses the transcriptional activity of a large number of neuronal genes in non-neuronal cells. It has been sugg...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.12.080
更新日期:2006-05-08 00:00:00
abstract::The paper focuses on such candidate gene polymorphisms that alter alcoholism-related intermediate phenotypes including: dopaminergic system polymorphic variants (DRD2 -141C Ins/Del in promoter region, exon 8 and DRD2 TaqI A and DAT 40bp VNTR genes polymorphisms) that cause predisposition to severe alcoholism (haplotyp...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.05.005
更新日期:2006-12-13 00:00:00
abstract::Iontophoretically applied 2-azido photoaffinity analogues of adenosine 5'-triphosphate (ATP) and adenosine 5'-monophosphate (AMP) have been tested on rat cerebral cortical neurons. 2-Azido-AMP and 2-azido-ATP had a powerful depressant action on the spontaneous firing of cortical neurons. Both compounds were equipotent...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(81)90058-6
更新日期:1981-01-01 00:00:00
abstract::The aim was to examine the effect of handball training on cognitive ability in elderly with mild cognitive impairment. A total of 60 elderly were randomly divided into training group (n=30) and control group (n=30). The mini-mental state examination (MMSE) score and abilities of daily living scale (ADL) score before, ...
journal_title:Neuroscience letters
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.neulet.2014.02.035
更新日期:2014-04-30 00:00:00