Abstract:
:To differentiate the opiate (naloxone) receptor and the enkephalin receptor in rat brain, we solubilized the receptor molecules by detergent and determined the molecular weights by gel filtration. The receptor preparation was bound to [3H] naloxone or [3H] Met5-enkephalin, and was solubilized by Triton X-100. On gel chromatography with a Sepharose 6B column, the agonist and the antagonist conformation of opioid receptors eluted as molecules with the molecular weights of 240,000, and 120,000 and with Stokes' radii of 5.5 nm and 4.3 nm, respectively. Further, it was also disclosed that Na+ was bound to the antagonist conformation of opioid receptors but not to the agonist conformation.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Ogawa N,Yamawaki Y,Kuroda H,Nukina I,Ofuji Tdoi
10.1016/0304-3940(81)90269-xsubject
Has Abstractpub_date
1981-12-11 00:00:00pages
205-9issue
2eissn
0304-3940issn
1872-7972pii
0304-3940(81)90269-Xjournal_volume
27pub_type
杂志文章abstract::This experiment was designed to simulate experimentally the specific parameters of geomagnetic activity that evoke epileptic seizures. The numbers of overt limbic seizures (rearing, paroxysmal forelimb clonus and falling) in a population of epileptic rats were recorded nightly for 65 successive days between 0200 and 0...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)13446-2
更新日期:1997-03-07 00:00:00
abstract::Previous studies suggest that neuropeptide FF (NPFF) plays a role in opiate dependence and subsequent abstinence syndrome. Endogenous NPFF also appears to play a role in opiate tolerance since third ventricle injection of IgG from NPFF antiserum selectively restores morphine sensitivity in morphine-tolerant rats. The ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90078-l
更新日期:1992-11-09 00:00:00
abstract::Phencyclidine (PCP) abuse is reaching alarming proportions. PCP has recently been shown to induce hypertensive encephalopathies, microvascular cerebrovasospasm and acute intracerebral hemorrhage. Since we have shown in vitro that cerebral vasospasms induced by PCP could be completely reversed, or prevented, by use of ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90505-4
更新日期:1990-05-18 00:00:00
abstract::A replication-defective recombinant adenoviral vector containing E. coli lacZ gene was injected into the gastrocnemius muscles of transgenic mice carrying mutant Cu/Zn superoxide dismutase (SOD1) gene and non-transgenic wild-type mice at 40 weeks of age. After 60 and 90 h of the injection, lacZ staining was observed a...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)02036-5
更新日期:2001-08-10 00:00:00
abstract::A retrograde tracer, Diamidino yellow, was injected into the hindlimb region of the supplementary motor area after intracortical microstimulation mapping in the Japanese monkey. As a result, a cluster of retrogradely labeled neurons was found in the caudal bank of the arcuate sulcus, which corresponds to the ventral p...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90629-7
更新日期:1994-04-25 00:00:00
abstract::Lowering of the pH of the superfusion solution (physiological phosphate buffers derived from normal Krebs solution) of mucosa-free muscle strips of the guinea-pig urinary bladder from 7.4 to 6 or 5, produced a marked increase of calcitonin gene-related peptide-like immunoreactivity (CGRP-LI) outflow (total evoked rele...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90435-c
更新日期:1990-06-22 00:00:00
abstract::Five picrotoxinin analogues were examined on GABA-gated chloride channels of an identifiable cockroach (Periplaneta americana) motor neurone (Df). Substitution of the bridgehead hydroxyl at the C-6 position of the picrotoxinin molecule by a fluorine atom (fluoropicrotoxinin) had little effect, whereas acetylation of t...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90606-8
更新日期:1994-04-25 00:00:00
abstract::We immunohistochemically examined the expression of glycogen synthase kinase-3beta (GSK-3beta) in the brains of Parkinson's disease (PD) patients. GSK-3beta was localized in punctate structures in the cytosol of subsets of neurons in the midbrain and upper pons. GSK-3beta was also localized in Lewy bodies (LBs) as was...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.10.104
更新日期:2009-01-09 00:00:00
abstract::We have recently reported fluctuations in the expression of the period repeat sequence (pp2.5) during light-dark cycles in the suprachiasmatic nucleus (SCN) of rat [Neurosci. Lett., 122 (1991) 113-116]. Presently, we performed in situ hybridization which shows that the fluctuation of pp2.5 expression continues during ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90379-y
更新日期:1993-12-12 00:00:00
abstract::The lysate of an immortalized monoclonal cell line derived from the striatum (X61) contains a dopaminergic stimulatory activity that is capable of increasing the dopamine content of an immortalized mouse mesencephalic cell line (MN9D) which expresses a dopaminergic phenotype. Purification of an isoamyl alcohol extract...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.11.021
更新日期:2005-03-07 00:00:00
abstract::Previous in vitro experiments have indicated that neuronal GABA aminotransferase (GABA-T) differs from extraneuronal GABA-T in sensitivity to certain enzyme inhibitors, including the antiepileptic drug valproate (VPA). In the present experiments, it was studied if GABA-T activity in whole tissue and synaptosomes prepa...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90407-c
更新日期:1993-10-01 00:00:00
abstract::Occasional repetitions embedded in a sequence of ever-changing sounds elicit a frontally negative event-related potential peaking between 100 and 200 ms from the onset of sound repetition (repetition negativity, RN). Three interpretations have been suggested for the emergence of RN: (1) RN is a mismatch negativity (MM...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.07.004
更新日期:2004-09-23 00:00:00
abstract::The mammalian circadian system contains both central and peripheral oscillators. It was reported that the rhythmic expressions of period homolog 2 (per2) mRNA in peripheral tissues were abolished by the suprachiasmatic nucleus (SCN) lesions. However, there are no reports that ventromedial hypothalamic (VMH) lesions ca...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2010.01.029
更新日期:2010-03-08 00:00:00
abstract::The KIBRA gene encodes a cytoplasmatic protein, a member of the signal transduction protein family, expressed mainly in the brain. Recent studies have implicated the involvement of a genetic variation in the KIBRA gene (T allele) in human memory in normal subjects and in the risk of developing Alzheimer's disease (AD)...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.03.008
更新日期:2008-05-09 00:00:00
abstract::Bereitschaftspotentials (BPs) preceding muscle relaxation and contraction were compared by using subdural electrodes which were implanted onto the right medial frontal surface in two patients with supplementary motor area (SMA) seizure. The applied movement paradigm (muscle relaxation and contraction tasks) was comple...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(98)00149-9
更新日期:1998-03-20 00:00:00
abstract::The effects of electrical stimulation of the central (CE) and basolateral (BL) amygdaloid nuclei on pancreatic exocrine secretion were tested in urethane-anesthetized rats. Electrical stimulation (0.1 mA, 1 ms, 40 Hz) of the CE significantly increased basal and hormone (secretin+cholecystokinin-8)-infused pancreatic e...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90352-2
更新日期:1994-03-14 00:00:00
abstract::Neuronal voltage-dependent P/Q Ca2+ channels are genetically abnormal in many cases of familial hemiplegic migraine and possibly associated with the more common forms of migraine with and without aura. Besides the brain, these channels are found in motor nerve endings where they control stimulation-induced acetylcholi...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(99)00820-4
更新日期:1999-12-10 00:00:00
abstract::In the present study, the origin of calcitonin gene-related peptide (CGRP) to the dorsal horn in the rat lumbar spinal cord is investigated. CGRP immunoreactivity is examined following multiple unilateral and bilateral dorsal rhizotomies and isolated cord preparations (spinal cords are isolated by transecting the cord...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90781-1
更新日期:1988-07-19 00:00:00
abstract::The magnocellular neurones of the supraoptic nucleus (SON) and paraventricular nucleus release neuropeptide from their axon terminals and also from their dendrites. In the axon terminals, swellings known as Herring bodies are responsible for the degradation of aged, unreleased large dense-cored vesicles (LDCVs) by lys...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2010.11.060
更新日期:2011-02-01 00:00:00
abstract::Mutations in the hairless (hr) gene of mice result in hair follicle and other epithelial defects. The hr gene is expressed at high levels in the brain where it probably participates in the survival and maintenance of some neuronal populations, but whether it also supports glial populations of the central nervous syste...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)02041-9
更新日期:2001-08-24 00:00:00
abstract::Infection of the snail Lymnaea stagnalis with the schistosome parasite Trichobilharzia ocellata results in inhibition of reproduction and in giant growth. Parasite-related effects on the neuroendocrine centres that control these processes were studied electrophysiologically. Haemolymph from infected snails reduced the...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90047-b
更新日期:1992-03-02 00:00:00
abstract::Temperature effects on ocular serotonin N-acetyltransferase (NAT) kinetics characteristics from Rana perezi have been studied with respect to tryptamine and serotonin as substrates. Monoamine oxidase (MAO) activity does not interfere in NAT assay at acceptoramine concentrations used in NAT kinetics characterization fr...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90667-a
更新日期:1993-05-28 00:00:00
abstract::Plasma corticosterone has been correlated with the susceptibility to animal hypnosis in male rabbits. Subjects (Ss) were considered susceptible when mean hypnosis duration was above 30 sec or when initial duration (duration of trial 1) was greater than 0. A positive significant correlation was found between mean and i...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(79)90006-5
更新日期:1979-03-01 00:00:00
abstract::Purkinje cell degeneration (pcd) mutant mice lose cerebellar Purkinje cells. It was found that pcd mice, contrary to normal mice, did not alternate spontaneously at any of 3 inter-trial intervals (15 s, 3 or 6 min). Deficits in spontaneous alternation are typical of animals with brain damage in areas important in spat...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(87)90479-4
更新日期:1987-10-05 00:00:00
abstract::Amyloid proteins are a group of proteins that can polymerize into cross beta-sheeted amyloid species. We have found that enhancing cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) formazan exocytosis is a common property of bioactive amyloid species formed from all of the amyloid proteins te...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.07.047
更新日期:2006-10-09 00:00:00
abstract::In fetal and adult human brains, calbindin immunoreactivity (CB-ir) of neostriatal neuropil showed inhomogeneous pattern. A mosaic of CB-ir neuropil patches matching the acetylcholinesterase (AChE)-reactive patches and most of the encapsulated cell-dense islands was distributed in a lighter stained background matrix. ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(96)13270-5
更新日期:1996-12-20 00:00:00
abstract::The type of glass used to fabricate patch pipettes influences the kinetic properties of single-channel currents activated by acetylcholine in Xenopus myocytes. In patches formed with hard glass pipettes, the opening rate decreased in a time-dependent manner. Such decrease was most pronounced for the low-conductance ch...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90312-6
更新日期:1988-05-16 00:00:00
abstract::To determine whether a trans-synaptic mechanism triggered the effects of reserpine on adrenomedullary mRNAs encoding the norepinephrine transporter and tyrosine hydroxylase, we administered 10 mg/kg reserpine to rats after unilateral splanchnicotomy, and examined their adrenal medullas using quantitative in situ hybri...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)11699-w
更新日期:1995-07-07 00:00:00
abstract::The roles of the endogenous adenosine on acetylcholine release via adenosine A1 receptor were investigated in rat cerebral cortex using brain microdialysis. Oral administration of KF15372 (8-dicyclopropylmethyl-1,3-dipropylxanthine), a novel selective adenosine A1 receptor antagonist, at doses of 1.25, 5, and 20 mg/kg...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(96)12632-x
更新日期:1996-05-17 00:00:00
abstract::Spontaneous action potentials were recorded longitudinally for 4-7 weeks from dissociated rat occipital cortex cells cultured on planar multi-electrode plates, during their development from isolated neurons into synaptically connected neuronal networks. Activity typically consisted of generalized bursts lasting up to ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2003.12.062
更新日期:2004-05-06 00:00:00