Differentiation of agonist conformation and antagonist conformation in multiple opioid receptors.

Abstract:

:To differentiate the opiate (naloxone) receptor and the enkephalin receptor in rat brain, we solubilized the receptor molecules by detergent and determined the molecular weights by gel filtration. The receptor preparation was bound to [3H] naloxone or [3H] Met5-enkephalin, and was solubilized by Triton X-100. On gel chromatography with a Sepharose 6B column, the agonist and the antagonist conformation of opioid receptors eluted as molecules with the molecular weights of 240,000, and 120,000 and with Stokes' radii of 5.5 nm and 4.3 nm, respectively. Further, it was also disclosed that Na+ was bound to the antagonist conformation of opioid receptors but not to the agonist conformation.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Ogawa N,Yamawaki Y,Kuroda H,Nukina I,Ofuji T

doi

10.1016/0304-3940(81)90269-x

subject

Has Abstract

pub_date

1981-12-11 00:00:00

pages

205-9

issue

2

eissn

0304-3940

issn

1872-7972

pii

0304-3940(81)90269-X

journal_volume

27

pub_type

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