当前位置： SCI文献检索 > BIOMACROMOLECULES期刊下所有文献 > Degradation of chitosans with a family 46 chitosanase from Streptomyces coelicolor A3(2).

Degradation of chitosans with a family 46 chitosanase from Streptomyces coelicolor A3(2).

Abstract：

:We have studied the degradation of well-characterized soluble heteropolymeric chitosans by a novel family 46 chitosanase, ScCsn46A from Streptomyces coelicolor A3(2), to obtain insight into the enzyme's mode of action and to determine its potential for production of different chitooligosaccharides. The degradation of both a fully deacetylated chitosan and a 32% acetylated chitosan showed a continuum of oligomeric products and a rapid disappearance of the polymeric fraction, which is diagnostic for a nonprocessive endomode of action. The kinetics of the degradation of the 32% acetylated chitosan demonstrated an initial rapid phase and a slower second phase, in addition to a third and even slower kinetic phase. The first phase reflects the cleavage of the glycosidic linkage between two deacetylated units (D-D), the primary products being fully deacetylated dimers, trimers, and tetramers, as well as longer oligomers with increasing degrees of acetylation. In the subsequent slower kinetic phases, oligomers with a higher degree of acetylated units (A) appear, including oligomers with A's at the reducing or nonreducing end, which indicate that there are no absolute preferences for D in subsites -1 and +1. After maximum degradation of the chitosan, the dimers DA and DD were the dominant products. The degradation of chitosans with varying degrees of acetylation to a maximum degree of scission showed that ScCsn46A could degrade all chitosan substrates extensively, although to decreasing degrees of scission with increasing F(A). The potential use of ScCsn46A to prepare fully deacetylated oligomers and more highly acetylated oligomers from chitosan substrates with varying degrees of acetylation is discussed.

journal_name

Biomacromolecules

journal_title

Biomacromolecules

authors

Heggset EB,Dybvik AI,Hoell IA,Norberg AL,Sørlie M,Eijsink VG,Vårum KM

doi

10.1021/bm1006745

subject

Has Abstract

pub_date

2010-09-13 00:00:00

pages

2487-97

issue

9

eissn

1525-7797

issn

1526-4602

journal_volume

11

pub_type

杂志文章

相关文献

BIOMACROMOLECULES文献大全
  • Deoxyguanosine phosphate mediated sacrificial bonds promote synergistic mechanical properties in nacre-mimetic nanocomposites.

    abstract：:We show that functionalizing polymer-coated colloidal nanoplatelets with guanosine groups allows synergistic increase of mechanical properties in nacre-mimetic lamellar self-assemblies. Anionic montmorillonite (MTM) was first coated using cationic poly(diallyldimethylammonium chloride) (PDADMAC) to prepare core-shell ...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm400056c

    authors： Martikainen L,Walther A,Seitsonen J,Berglund L,Ikkala O

    更新日期：2013-08-12 00:00:00

  • Environmentally Benign Nanoantibiotics with a Built-in Deactivation Switch Responsive to Natural Habitats.

    abstract：:The massive use of antibiotics in healthcare and agriculture has led to their artificial accumulation in natural habitats, which risks the structure and function of the microbial communities in ecosystems, threatens food and water security, and accelerates the development of resistome. Ideally, antibiotics should rema...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.0c00163

    authors： Zheng W,Anzaldua M,Arora A,Jiang Y,McIntyre K,Doerfert M,Winter T,Mishra A,Ma H,Liang H

    更新日期：2020-06-08 00:00:00

  • The Influence of Biomaterials on Cytokine Production in 3D Cultures.

    abstract：:As a result of improved relevance to in vivo physiology, in vitro studies are increasingly performed in diverse, three-dimensional (3D) biomaterials. However, material-cell type pairing effects on cytokine availability remain unclear. We cultured five cell types in agarose, alginate, collagen, Matrigel, or RGD-functio...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.6b01469

    authors： Regier MC,Montanez-Sauri SI,Schwartz MP,Murphy WL,Beebe DJ,Sung KE

    更新日期：2017-03-13 00:00:00

  • Production of extracellular glycogen by Pseudomonas fluorescens: spectroscopic evidence and conformational analysis by biomolecular recognition.

    abstract：:Glycogen is mainly found as the principal storage form of glucose in cells. Many bacteria are able to synthesize large amounts of glycogen under unfavorable life conditions. By combining infrared spectroscopy, single molecule force spectroscopy (SMFS) and immuno-staining technique, we evidenced that planktonic P. flu...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm300497c

    authors： Quilès F,Polyakov P,Humbert F,Francius G

    更新日期：2012-07-09 00:00:00

  • Influence of enzymatic specificity on the behavior of ephemeral gels.

    abstract：:Ephemeral gels, called Enzgels, successively undergo sol-gel and then gel-sol transition under the action of two antagonistic enzymes, transglutaminase and protease. Molecular and macroscopic properties of Enzgels are directly dependent on the enzymatic activities and their ratios. This work studies the characteristic...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm700601n

    authors： Picard J,Giraudier S,Larreta-Garde V

    更新日期：2008-01-01 00:00:00

  • Kinetics of protein-protein complex coacervation and biphasic release of salbutamol sulfate from coacervate matrix.

    abstract：:Turbidimetric titration was used to initiate associative intermolecular interactions between a pair of protein molecules, gelatin-A and gelatin-B, having complementary charges that led to pH-induced liquid-liquid phase separation and the formation of complex coacervate. The stoichiometric binding ratio was found to be...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm801160s

    authors： Tiwari A,Bindal S,Bohidar HB

    更新日期：2009-01-12 00:00:00

  • Artificial forisomes are ideal models of forisome assembly and activity that allow the development of technical devices.

    abstract：:Forisomes are protein polymers found in leguminous plants that have the remarkable ability to undergo reversible "muscle-like" contractions in the presence of divalent cations and in extreme pH environments. To gain insight into the molecular basis of forisome structure and assembly, we used confocal laser scanning mi...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm3008499

    authors： Groscurth S,Müller B,Schwan S,Menzel M,Diekstall F,Senft M,Kendall A,Kommor BA,Neumann U,Kalischuk M,Kawchuk LM,Krzyzanek V,Heilmann A,Stubbs G,Twyman RM,Prüfer D,Noll GA

    更新日期：2012-10-08 00:00:00

  • Synthesis and properties of degradable poly(urethane urea)s to be used for ligament reconstructions.

    abstract：:In the present study we describe the synthesis, wet spinning, mechanical testing, and degradation of poly(urethane urea)s (PUURs) intended for clinical use in anterior cruciate ligament (ACL) reconstruction. The effects of soft segment chemical composition and molar mass and the kind of diamine chain extender on the m...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm025535u

    authors： Gisselfält K,Edberg B,Flodin P

    更新日期：2002-09-01 00:00:00

  • Injectable Shape-Holding Collagen Hydrogel for Cell Encapsulation and Delivery Cross-linked Using Thiol-Michael Addition Click Reaction.

    abstract：:Injectable hydrogels based on extracellular matrix-derived polymers show much promise in the field of tissue engineering and regenerative medicine. However, the hydrogels reported to date have at least one characteristic that limits their potential for clinical use, such as excessive swelling, complicated and potentia...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.9b00769

    authors： Pupkaite J,Rosenquist J,Hilborn J,Samanta A

    更新日期：2019-09-09 00:00:00

  • Peptides at the Interface: Self-Assembly of Amphiphilic Designer Peptides and Their Membrane Interaction Propensity.

    abstract：:Self-assembling amphiphilic designer peptides have been successfully applied as nanomaterials in biomedical applications. Understanding molecular interactions at the peptide-membrane interface is crucial, since interactions at this site often determine (in)compatibility. The present study aims to elucidate how model m...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.6b01089

    authors： Kornmueller K,Lehofer B,Meindl C,Fröhlich E,Leitinger G,Amenitsch H,Prassl R

    更新日期：2016-11-14 00:00:00

  • Triblock Copolymer Nanovesicles for pH-Responsive Targeted Delivery and Controlled Release of siRNA to Cancer Cells.

    abstract：:New pH-responsive polymersomes for active anticancer oligonucleotide delivery were prepared from triblock copolymers. The delivery systems were formed by two terminal hydrophilic blocks, PEG and polyglycerolmethacrylate (poly-GMA), and a central weakly basic block, polyimidazole-hexyl methacrylate (poly-ImHeMA), which...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.5b00286

    authors： Gallon E,Matini T,Sasso L,Mantovani G,Armiñan de Benito A,Sanchis J,Caliceti P,Alexander C,Vicent MJ,Salmaso S

    更新日期：2015-07-13 00:00:00

  • Polymeric Materials for Eye Surface and Intraocular Applications.

    abstract：:Ocular applications of polymeric materials have been widely investigated for medical diagnostics, treatment, and vision improvement. The human eye is a vital organ that connects us to the outside world so when the eye is injured, infected, or impaired, it needs immediate medical treatment to maintain clear vision and ...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.0c01525

    authors： Karayilan M,Clamen L,Becker ML

    更新日期：2021-01-06 00:00:00

  • Synthesis and Characterization of Silk Ionomers for Layer-by-Layer Electrostatic Deposition on Individual Mammalian Cells.

    abstract：:Nanocoating of individual mammalian cells with polymer layers has been of increasing interest in biotechnology and biomedical engineering applications. Electrostatic layer-by-layer (LbL) deposition of polyelectrolytes on negatively charged cell surfaces has been utilized for cell nanocoatings using synthetic or natura...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.0c00523

    authors： Hasturk O,Sahoo JK,Kaplan DL

    更新日期：2020-07-13 00:00:00

  • Injectable polymeric microspheres with X-ray visibility. Preparation, properties, and potential utility as new traceable bulking agents.

    abstract：:The copolymer of methyl methacrylate (MMA) and 2-[2',3',5'-triiodobenzoyl]oxoethyl methacrylate (1), ratio 3:1 (mass:mass), was prepared via a free-radical polymerization in bulk. The copolymer (M(w) = 97.8 kD and M(n) = 41.5 kD) was dissolved in chloroform and subsequently transformed into beads with a diameter in th...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm030002m

    authors： Saralidze K,Aldenhoff YB,Knetsch ML,Koole LH

    更新日期：2003-05-01 00:00:00

  • Structures and structure-activity relationships of three mitogenic and complement fixing pectic arabinogalactans from the malian antiulcer plants Cochlospermum tinctorium A. Rich and Vernonia kotschyana Sch. Bip. ex Walp.

    abstract：:Structures of three pectic arabinogalactans, one from Vernonia kotschyana (Vk2a) and two from Cochlospermum tinctorium (Ct50A1 and Ct50A2), and their complement fixation and induction of B cell proliferation in vitro were compared. The polysaccharide Vk2a expressed potent biological activity in both assays compared wi...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm050355g

    authors： Nergard CS,Kiyohara H,Reynolds JC,Thomas-Oates JE,Matsumoto T,Yamada H,Patel T,Petersen D,Michaelsen TE,Diallo D,Paulsen BS

    更新日期：2006-01-01 00:00:00

  • Polarity and motility of large polymer-actin complexes.

    abstract：:The polarity of polymer-actin complexes obtained by mixing F-actin with synthetic polymers carrying positive charges such as poly(L-lysine), x,y-ionene bromide polymers, and poly(N-[3-(dimethylamino)propyl]acrylamide) (PDMAPAA-Q) have been investigated. Actin complexes formed with poly(L-lysine) and PDMAPAA-Q, which c...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm0494038

    authors： Kakugo A,Shikinaka K,Takekawa N,Sugimoto S,Osada Y,Gong JP

    更新日期：2005-03-01 00:00:00

  • Tumor Microenvironmental pH and Enzyme Dual Responsive Polymer-Liposomes for Synergistic Treatment of Cancer Immuno-Chemotherapy.

    abstract：:Despite recent advances in tumor treatment through cancer immunotherapy, the efficacy of this approach remains to be improved. Looking forward to high rates of objective clinical response, cancer immunotherapy combined with chemotherapy has gained increasing attention recently. Here, we constructed liposomes with matr...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.8b01510

    authors： Liu Y,Chen XG,Yang PP,Qiao ZY,Wang H

    更新日期：2019-02-11 00:00:00

  • Inulin-D-α-tocopherol succinate (INVITE) nanomicelles as a platform for effective intravenous administration of curcumin.

    abstract：:The aim of this work was to evaluate the potential of INVITE-based nanomicelles, an amphiphilic polymer constituted by inulin (INU) and vitamin E (VITE), as a platform for improving the biopharmaceutical properties of hydrophobic drugs. For this purpose, curcumin was selected as a model and curcumin-INVITE nanomicelle...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm501616e

    authors： Tripodo G,Pasut G,Trapani A,Mero A,Lasorsa FM,Chlapanidas T,Trapani G,Mandracchia D

    更新日期：2015-02-09 00:00:00

  • Hyaluronic acid based materials for scaffolding via two-photon polymerization.

    abstract：:Hydrogels are able to mimic the basic three-dimensional (3D) biological, chemical, and mechanical properties of native tissues. Since hyaluronic acid (HA) is a chief component of human extracellular matrix (ECM), it represents an extremely attractive starting material for the fabrication of scaffolds for tissue engine...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm401712q

    authors： Kufelt O,El-Tamer A,Sehring C,Schlie-Wolter S,Chichkov BN

    更新日期：2014-02-10 00:00:00

  • Biocompatible micro-gel particles from cross-linked casein micelles.

    abstract：:The stability of internally cross-linked casein micelles against disruption by urea (which disrupts hydrogen bonds and hydrophobic interactions) and trisodium citrate (which sequesters micellar calcium phosphate) was investigated. Addition of urea (0-6 mol L-1) and/or citrate (0-50 mmol L-1) progressively reduced the ...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm061070m

    authors： Huppertz T,Smiddy MA,de Kruif CG

    更新日期：2007-04-01 00:00:00

  • In situ controlled release of rhBMP-2 in gelatin-coated 3D porous poly(ε-caprolactone) scaffolds for homogeneous bone tissue formation.

    abstract：:In tissue engineering, incorporation of bone morphogenetic protein-2 (BMP-2) into biomaterial scaffolds is an attractive strategy to stimulate bone repair. However, suboptimal release of BMP-2 remains a great concern, which may cause unfavorable bone formation as well as severe inflammation. In this study, genipin-cro...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm401309u

    authors： Zhang Q,Tan K,Zhang Y,Ye Z,Tan WS,Lang M

    更新日期：2014-01-13 00:00:00

  • Polycarbonates with Potent and Selective Antimicrobial Activity toward Gram-Positive Bacteria.

    abstract：:The resistance developed by life-threatening bacteria toward conventional antibiotics has become a major concern in public health. To combat antibiotic resistance, there has been a significant interest in the development of antimicrobial cationic polymers due to the ease of synthesis and low manufacturing cost compare...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.6b01385

    authors： Nimmagadda A,Liu X,Teng P,Su M,Li Y,Qiao Q,Khadka NK,Sun X,Pan J,Xu H,Li Q,Cai J

    更新日期：2017-01-09 00:00:00

  • Efficient manipulation of nanoparticle-bound DNA via restriction endonuclease.

    abstract：:As a programmable biopolymer, DNA has shown great potential in the fabrication and construction of nanometer-scale assemblies and devices. In this report, we described a strategy for efficient manipulation of gold nanoparticle-bound DNA using restriction endonuclease. The digestion efficiency of this restriction enzym...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm060517o

    authors： Qin WJ,Yung LY

    更新日期：2006-11-01 00:00:00

  • Structural and Mechanical Roles for the C-Terminal Nonrepetitive Domain Become Apparent in Recombinant Spider Aciniform Silk.

    abstract：:Spider aciniform (or wrapping) silk is the toughest of the seven types of spider silks/glue due to a combination of high elasticity and strength. Like most spider silk proteins (spidroins), aciniform spidroin (AcSp1) has a large core repetitive domain flanked by relatively short N- and C-terminal nonrepetitive domains...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.7b01057

    authors： Xu L,Lefèvre T,Orrell KE,Meng Q,Auger M,Liu XQ,Rainey JK

    更新日期：2017-11-13 00:00:00

  • Ultrasensitive Detection of MicroRNA in Tumor Cells and Tissues via Continuous Assembly of DNA Probe.

    abstract：:Nucleic acids have been engineered to participate in a wide variety of tasks. Among them, the enzyme-free amplification modes, enzyme-free DNA circuits (EFDCs), and hybridization chain reactions (HCRs) have been widely applied in a series of studies of bioanalysis. We demonstrated here an ultrasensitive hairpin probe-...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.5b00959

    authors： Liao Y,Fu Y,Wu Y,Huang R,Zhou X,Xing D

    更新日期：2015-11-09 00:00:00

  • Altered Thermal and Mechanical Properties of Spruce Galactoglucomannan Films Modified with an Etherification Reaction.

    abstract：:Native hemicellulose lacks many of the properties that make fossil fuel-based polymers excellent for use in today's industrial products and processes. The mechanical and thermal properties of the hemicellulose can, however, be modified, and its processability increased. We functionalized galactoglucomannan to lower it...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/acs.biomac.9b01730

    authors： Härdelin L,Bernin D,Börjesson M,Ström A,Larsson A

    更新日期：2020-05-11 00:00:00

  • Block copolymer of polyphosphoester and poly(L-lactic acid) modified surface for enhancing osteoblast adhesion, proliferation, and function.

    abstract：:Surface modification is often needed in tissue engineering to enhance the interaction between cells and synthetic materials and improve the cytocompatibility and cellular functions. In this study, block copolymers of poly(L-lactic acid) and poly(ethyl ethylene phosphate) (PLLA-b-PEEP) were synthesized and used to modi...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm900390k

    authors： Yang XZ,Sun TM,Dou S,Wu J,Wang YC,Wang J

    更新日期：2009-08-10 00:00:00

  • Resilient bioresorbable copolymers based on trimethylene carbonate, L-lactide, and 1,5-dioxepan-2-one.

    abstract：:The new combinations of monomers presented in this work were evaluated in order to create an elastic material for potential application in soft tissue engineering. Thermoplastic elastomers (TPE) of trimethylene carbonate (TMC) with L-lactide (LLA) and 1,5-dioxepan-2-one (DXO) have been synthesized using a cyclic five-...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm060081c

    authors： Andronova N,Albertsson AC

    更新日期：2006-05-01 00:00:00

  • Electrospun nanofibrous mats containing quaternized chitosan and polylactide with in vitro antitumor activity against HeLa cells.

    abstract：:Nanofibrous materials containing the antitumor drug doxorubicin hydrochloride (DOX) were easily prepared using a one-step method by electrospinning of DOX/poly(L-lactide-co-D,L-lactide) (coPLA) and DOX/quaternized chitosan (QCh)/coPLA solutions. The pristine and DOX-containing mats were characterized by ATR-FTIR and X...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm100285n

    authors： Ignatova MG,Manolova NE,Toshkova RA,Rashkov IB,Gardeva EG,Yossifova LS,Alexandrov MT

    更新日期：2010-06-14 00:00:00

  • Global structures of high methoxyl pectin from solution and in gels.

    abstract：:Images of high methoxyl orange pectin deposited from solution and high methoxyl sugar acid gels (HMSAG) were obtained by atomic force microscopy (AFM) in the tapping mode. For the first time, images of pectin deposited from water revealed that the transition from pectin networks to individual molecules or aggregates t...

    journal_title：Biomacromolecules

    pub_type： 杂志文章

    doi：10.1021/bm0607729

    authors： Fishman ML,Cooke PH,Chau HK,Coffin DR,Hotchkiss AT Jr

    更新日期：2007-02-01 00:00:00