Abstract:
:Recent studies have reported that cancer stem cells (CSCs) could be isolated from solid cancer cell lines, in which the purity of CSCs was higher than that from tumor tissues. Separation of CSCs from leukemic cell lines was rarely reported. In this study, CD34(+)CD38(-)stem-like cell subsets in human KG-1a leukemic cell line were enriched by cytotoxic agent 5-fluorouracil (5-FU). After 4 days incubation of KG-1a cell line with 5-FU (50 microg/ml), the CD34(+)CD38(-) subpopulation of cell lines was enriched more than 10 times. The enriched cells had proliferate potential in vitro, low level of RNA transcription and Hoechst 33342 dye efflux ability, accompanied by high expression of ATP-binding cassette transporter protein ABCG2. Our findings suggest that treatment with 5-FU offers an easy method to isolate leukemic stem-like subpopulation. It can facilitate studies of leukemic stem cell biology and the development of new therapeutic strategies.
journal_name
Int J Biol Scijournal_title
International journal of biological sciencesauthors
Zhang L,Yang S,He YJ,Shao HY,Wang L,Chen H,Gao YJ,Qing FX,Chen XC,Zhao LY,Tan Sdoi
10.7150/ijbs.6.419subject
Has Abstractpub_date
2010-08-03 00:00:00pages
419-27issue
5issn
1449-2288journal_volume
6pub_type
杂志文章abstract::Dihydropyrimidinase-related protein 4 (Dpysl4) is a known regulator of hippocampal neuron development. Here, we report that Dpysl4 is involved in growth regulation, polarization and differentiation of dental epithelial cells during tooth germ morphogenesis. A reduction in Dpysl4 gene expression in the tooth germ produ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5510
更新日期:2013-04-26 00:00:00
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journal_title:International journal of biological sciences
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journal_title:International journal of biological sciences
pub_type: 杂志文章
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更新日期:2017-01-11 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7242
更新日期:2013-11-05 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.8754
更新日期:2014-03-22 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7367
更新日期:2013-11-21 00:00:00
abstract:BACKGROUND:Increasing evidences suggest that innate immunity is involved in cerebral ischemia-reperfusion (I/R) injury, but the liable innate immune receptors have not been completely elucidated. Here, we explored the role of the nucleotide-binding oligomerization domain (NOD)2, a member of the cytosolic NOD-like recep...
journal_title:International journal of biological sciences
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更新日期:2015-03-25 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7251
更新日期:2013-09-25 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.15165
更新日期:2016-06-24 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.24681
更新日期:2018-04-05 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.4038
更新日期:2012-01-01 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.9886
更新日期:2014-09-10 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.27173
更新日期:2018-08-06 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.25200
更新日期:2018-06-03 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.3.237
更新日期:2007-03-02 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.837
更新日期:2011-01-01 00:00:00
abstract::Non-coding RNAs (ncRNAs) are transcribed from genome but not translated into proteins. Many ncRNAs are key regulators of plants growth and development, metabolism and stress tolerance. In order to make the web-based ncRNA resources for plant science research be more easily accessible and understandable, we made a comp...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.24593
更新日期:2018-05-22 00:00:00
abstract::Thermal injury (TI) with septic complications continues to be a serious clinical problem. One of the main concerns in such patients is immunosuppression related to functional derangements in intestinal CD4+ T lymphocytes. Extensive previous studies in thermal injury/septic patients and animal models of thermal injury/...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.3.393
更新日期:2007-09-12 00:00:00
abstract::Basigin is a highly glycosylated transmembrane protein that is expressed in a broad range of tissues and is involved in a number of physiological and pathological processes. However, the in vivo role of basigin remains unknown. To better understand the physiological and pathological functions of basigin in vivo, we ge...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.6818
更新日期:2013-12-13 00:00:00
abstract::The genetic landscape of pancreatic cancer shows nearly ubiquitous mutations of K-RAS. However, oncogenic K-Ras(mt) alone is not sufficient to lead to pancreatic ductal adenocarcinoma (PDAC) in either human or in genetically modified adult mouse models. Many stimulants, such as high fat diet, CCK, LPS, PGE2 and others...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.15020
更新日期:2016-01-28 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.2.110
更新日期:2006-01-01 00:00:00
abstract::Dendritic cells (DCs) are the most potent specialized antigen-presenting cells as now known, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. Immunologically, the motilities and T cell activation capabilities of DCs are closely related to the resulting immune respon...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.33412
更新日期:2019-05-20 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.33733
更新日期:2019-09-07 00:00:00
abstract::Farrerol has been shown to have antioxidative potential via Nrf2 activation, which in turn is involved in the prevention of hepatotoxicity. The purpose of the current study was to explore the protective effect of farrerol against acetaminophen-induced hepatotoxicity and its underlying mechanisms. Mice were used to eva...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.30677
更新日期:2019-01-29 00:00:00
abstract::Pacemaker activity of the sinoatrial node has been studied extensively in various animal species, but is virtually unexplored in man. As such, it is unknown whether the fast sodium current (I(Na)) plays a role in the pacemaker activity of the human sinoatrial node. Recently, we had the unique opportunity to perform pa...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5.201
更新日期:2009-01-01 00:00:00
abstract::Epithelial-to-mesenchymal transition (EMT) is a key process in EGFR-TKI resistance but the detailed mechanism is largely unknown. We aim to evaluate the role of interleukin-6 (IL-6) and insulin-like growth factor-1 receptor (IGR-1R) in EMT in non-small cell lung cancer (NSCLC). We used IL-6 to induce EMT in EGFR-TKI s...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.31999
更新日期:2019-07-20 00:00:00
abstract::When pathogenic stresses are recognized by innate immune cells, inflammasomes are assembled and caspase-1 is activated, resulting in the conversion of pro-IL-18 into mature IL-18. Because natural killer (NK) cells express IL-18 receptors, IL-18 may play roles in immune functions of NK cells. In the present study, we e...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.22809
更新日期:2018-03-09 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.12815
更新日期:2016-02-12 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.27411
更新日期:2018-07-13 00:00:00
abstract::MicroRNA-370 (miR-370) has been observed to act as a tumor suppressor through the targeting of different proteins in a variety of tumors. Our previous study indicated that miR-370 was able to target forkhead box protein M1 (FOXM1) to inhibit cell growth and metastasis in human osteosarcoma cells. In this study, we rep...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.19032
更新日期:2017-04-10 00:00:00