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The silencing of RECK gene is associated with promoter hypermethylation and poor survival in hepatocellular carcinoma.

Abstract：

BACKGROUND:To evaluate the promoter methylation status of RECK gene and mRNA expression in patients with hepatocellular carcinoma (HCC). METHODS:We analyzed RECK methylation by MSP, and RECK mRNA by real-time PCR in 74 HCC. The liver cell lines (7721, Chang and Hep-G2) were treated with 5-Aza-CdR and TSA. RESULTS:RECK mRNA were lower in HCC tissues (Mean (-∆Ct) = -3.29) than that in Non-Hcc tissues (Mean (-∆Ct) = -2.42). Expression of RECK was elevated in only 24 (32.43%) of the 74 HCC patients but decreased (-∆∆Ct<0) in 50 (67.57%) of the patients. RECK promoter was hypermethylated in 55.4% (41/74) of HCCs, and in only 17.6% (13/74) of Non-Hcc samples. RECK mRNA were lower in HCC patients with hypermethylation (∆MI>=0.5) (Mean (-∆∆Ct) = -1.75) than those with demethylation (∆MI<0.5) (Mean (-∆∆Ct) = 0.05), and there is a decreased tendency for RECK mRNA in HCC patients with promoter hypermethylation (p = 0.002). There was a significantly correlation found between RECK mRNA and poor survival after surgery. After treated by 5-Aza-CdR and TSA, we found that RECK mRNA induced different changes in 7721, Chang and Hep-G2 cells. And RECK demethylation also induced by epigenetic inhibitors. CONCLUSION:The results suggested that the hypermethylation may lead to promoter silencing of RECK mRNA and associated with poor survival in HCC.

journal_name

Int J Biol Sci

journal_title

International journal of biological sciences

authors

Zhang C,Ling Y,Zhang C,Xu Y,Gao L,Li R,Zhu J,Fan L,Wei L

doi

10.7150/ijbs.4038

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

451-8

issue

4

issn

1449-2288

pii

ijbsv08p0451

journal_volume

8

pub_type

杂志文章

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