当前位置： SCI文献检索 > International Journal of Biological Sciences期刊下所有文献 > The blunt trichoid sensillum of female mosquitoes, Anopheles gambiae: odorant binding protein and receptor types.

The blunt trichoid sensillum of female mosquitoes, Anopheles gambiae: odorant binding protein and receptor types.

Abstract：

:In order to find a blood host and to select appropriate oviposition sites female Anopheles gambiae mosquitoes rely on olfactory cues which are sensed by olfactory sensory neurons (OSNs) located within morphologically different sensilla hairs. While the sharp type trichoid sensilla are most abundant and intensely studied, the striking blunt type trichoid sensilla exist only in small numbers and their specific function is unknown. It has been suggested that they may play a role in the detection of chemical cues indicating oviposition sites. With the aim of identifying molecular elements in blunt type trichoid sensilla, which may be relevant for chemosensory function of this sensillum type, experiments were performed which include whole mount fluorescence in situ hybridization (WM-FISH) and fluorescence immunohistochemistry (WM-FIHC). The studies were concentrated on odorant binding proteins (AgOBPs) and odorant receptors (AgORs). WM-FISH approaches using a probe for the plus-C class AgOBP47 led to the labeling of cells, which resembled in number and antennal distribution pattern the blunt type trichoid sensilla. Moreover, WM-FIHC with an antiserum for AgOBP47 allowed to assign the AgOBP47-expressing cells to blunt type trichoid sensilla and to allocate the protein within the sensillum hair shafts. The result of double WM-FISH-experiments and combined WM-FIHC/FISH approaches indicated that the AgOBP47-expressing cells are co-localized with cells, which express AgOR11, AgOR13 and AgOR55. In addition, it turned out that the two receptor types AgOR13 and AgOR55 are co-expressed in the same cells. Together, the results indicate that the blunt type trichoid sensilla contain a characteristic binding protein, plus-C AgOBP47, in the sensillum lymph and two sensory neurons, one cell which express the odorant receptor AgOR11 and a second cell which express the receptor types AgOR13 and AgOR55. The expression of characteristic chemosensory elements in blunt type trichoid sensilla supports the notion that this sensillum type is involved in sensing distinct odorous compounds.

journal_name

Int J Biol Sci

journal_title

International journal of biological sciences

authors

Schultze A,Breer H,Krieger J

doi

10.7150/ijbs.8754

subject

Has Abstract

pub_date

2014-03-22 00:00:00

pages

426-37

issue

issn

1449-2288

pii

ijbsv10p0426

journal_volume

pub_type

杂志文章

在线工具

The blunt trichoid sensillum of female mosquitoes, Anopheles gambiae: odorant binding protein and receptor types.