Abstract：
:Fluorescent tags and fluorophore-conjugated molecular probes have been extensively employed in histological studies to demonstrate nanoparticle distribution in inner ear cell populations. However, autofluorescence that exists in the rodent cochleae disturbs visualization of the fluorescent tags and fluorophore labeling. In the present work, we aimed to improve the visualization of fluorescently tagged nanoparticles and fluorophore-labeled molecular probes by treatment with CuSO(4) to quench autofluorescence in the rat inner ear. The in vivo study was performed on eight- to nine-month-old rats using confocal laser scanning microscopy, and the in vitro study was carried out with DiI-tagged poly(ethylene glycol) and poly(capro-lactone) polymersomes and different fluorescent-labeling agents using a spectrofluorometer. The nanoparticles were intratympanically administered using either an osmotic pump or transtympanic injection. Abundant autofluorescence was detected in spiral ganglion cells (SGCs), stria marginal cells, spiral ligament fibrocytes (SL) and the subcuticular cytoplasm of inner hair cells (IHCs). Sparsely distributed faint autofluorescence was also visualized in outer hair cells (OHCs). The autofluorescence was eliminated by treatment with 1 mM CuSO(4) (in 0.01 M ammonium acetate buffer) for 70-90 min, while the fluorescent tag in the nanoparticle was absolutely preserved and the labeling fluorescence signals of the molecular probes were mostly retained.
journal_name
Hear Resjournal_title
Hearing researchauthors
Zhang Y,Zhang W,Johnston AH,Newman TA,Pyykkö I,Zou Jdoi
10.1016/j.heares.2010.07.006subject
Has Abstractpub_date
2010-10-01 00:00:00pages
1-11issue
1-2eissn
0378-5955issn
1878-5891pii
S0378-5955(10)00333-3journal_volume
269pub_type
杂志文章相关文献
HEARING RESEARCH文献大全abstract：:The mechanisms underlying the ototoxicity of ethacrynic acid (EA) are not fully understood. Previous studies have focused on morphologic and enzymatic changes in the stria vascularis. The current experiment shows that one of the earliest effects of EA is ischemia, resulting from impaired blood flow in vessels supplyin...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(02)00585-3
更新日期：2002-11-01 00:00:00
abstract：:Input-specific remodeling is observed both in the primary auditory cortex (AI) and the ventral division of the medial geniculate body of the thalamus (MGBv) through motivation such as learning. Here, we show the role of AI in the MGBv remodeling induced by the electrical stimulation (ES) of the central division of the...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2015.06.016
更新日期：2015-10-01 00:00:00
abstract：:The goal of the current study was to characterize the normative features of wideband acoustic immittance in children for describing the functional maturation of the middle ear in 5 to 12-year-old children. Absorbance and group delay were measured in adults and three groups of children, 5-6, 7-9 and 10-12-year-olds, in...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2016.10.029
更新日期：2017-02-01 00:00:00
abstract：:Biochemical and pharmacological evidence support a role for nitric oxide (NO) and glutathione (GSH) in the cochlea. GSH combines with NO in tissue to form nitrosoglutathione (GSNO) that can act as a storage form for GSH and NO. Therefore, we tested GSNO on sound-evoked responses of the cochlea (cochlear microphonic, C...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(97)00065-8
更新日期：1997-08-01 00:00:00
abstract：:In ears in which cochlear efferent pathways were cut and with testing done under anaesthetic conditions that preclude middle ear muscle activity (so as to examine the "intrinsic" effects of loud sound on the cochlea without any confounding effect of efferent pathways to the auditory periphery), atraumatic background w...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2005.12.006
更新日期：2006-03-01 00:00:00
abstract：:Nitric oxide (NO) hyperpolarizes vascular smooth muscle cells and dilates blood vessels of various beds, but little is known on cochlear vasculatures. Using in vitro preparations of the spiral modiolar artery (SMA), intracellular electrical recording and labeling techniques, we found that the NO donor DPTA-NONOate (10...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(02)00497-5
更新日期：2002-09-01 00:00:00
abstract：:The dose-response ototoxic effects of cisplatin were studied in guinea pigs. Loss of Preyer reflex and suppression of the N1 amplitude occurred in cisplatin-treated animals and was described as dose-related. Drug-induced hair cell damage, as observed with scanning electron microscopy, occurred sporadically throughout ...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(87)90104-3
更新日期：1987-01-01 00:00:00
abstract：:Inner and outer hair cells were mechanically isolated from the guinea pig cochlea and subjected to stimuli known to induce shape changes in outer hair cells. Depolarization by 70 mM KCl which causes osmotic swelling of outer hair cells also swelled inner hair cells by approximately 8% of their volume. The application ...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(91)90202-k
更新日期：1991-03-01 00:00:00
abstract：:A large sample of threshold tuning curves of single units from various levels of the auditory pathway was used in order to examine the relationship between best frequency and a measure of sharpness of tuning. This measure is simply the tuning curve bandwidth at 20 dB above threshold on a square-root scale of frequency...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(83)90070-9
更新日期：1983-09-01 00:00:00
abstract：:Voice emotion is a fundamental component of human social interaction and social development. Unfortunately, cochlear implant users are often forced to interface with highly degraded prosodic cues as a result of device constraints in extraction, processing, and transmission. As such, individuals with cochlear implants ...
journal_title：Hearing research
pub_type： 杂志文章,评审
doi：10.1016/j.heares.2017.01.006
更新日期：2017-09-01 00:00:00
abstract：:In a previous study, we have demonstrated the presence of two adenosine receptor (AR) subtypes, namely A1 and A3AR, in the chinchilla cochlea. One or both of these receptors couple to activation of antioxidant enzymes, with resulting decreases in lipid peroxidation. The chemotherapeutic agent, cisplatin, was shown to ...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(97)00103-2
更新日期：1997-09-01 00:00:00
abstract：:The effect of a preceding (adapter) sound on the spatial discrimination of two subsequent, successively presented (target) sounds was tested in the horizontal plane. The adapter and the first target were located in front of the subject or 30 degrees to the right of the midline; both sounds were presented either at the...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2003.12.020
更新日期：2004-05-01 00:00:00
abstract：:Immunophilin FK binding protein-12 (FKBP-12), the soluble receptor for the immunosuppressant drug FK506, is involved in a number of neuronal activities including increased nerve regeneration in the peripheral nervous system and enhanced recovery in animal models of neurodegenerative diseases. In addition, FKBP-12 is t...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(01)00319-7
更新日期：2001-09-01 00:00:00
abstract：:When using bone vibrator transducers for clinical measurements, the transfer of energy from the bone driver depends on the impedance match between the driver and the load (human mastoid or otherwise) to which the driver will be applied. Current clinical calibration methods are incapable of quantifying this impedance m...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2010.02.013
更新日期：2010-05-01 00:00:00
abstract：:The basilar papilla of the Barn Owl's (Tyto alba) cochlea was found to be 9.5-11.5 mm long. Histological examination revealed that the sensory hair cells had a characteristic distribution: The proximal half contained mostly typical short cells; tall hair cells were present only on the distal half along with many short...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(85)90068-1
更新日期：1985-03-01 00:00:00
abstract：:A number of studies have shown that the ear can be protected from sound over-exposure, either by activating the cochlear efferent system, or by sound 'conditioning' in which the role of the efferent system is less certain. To study more definitively the molecular basis of deliberately induced cochlear protection from ...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(01)00265-9
更新日期：2001-06-01 00:00:00
abstract：:The possible presence of different types of delayed rectifier K+ current (I(K)) was studied in vestibular hair cells of frog semicircular canals. Experiments were performed in thin slice preparations of the whole crista ampullaris and recordings were made using the whole-cell patch-clamp technique. We found that an ap...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(99)00097-0
更新日期：1999-09-01 00:00:00
abstract：:Sensorineural hearing loss is the most common sensory deficit worldwide, yet our understanding of the underlying pathophysiology is limited by the challenges of access to the inner ear in a safe and reliable manner. We present a novel microneedle device for trans-round window membrane liquid biopsy, which utilizes con...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2019.06.004
更新日期：2019-09-15 00:00:00
abstract：:Auditory thresholds were measured for 18 ears from 13 rhesus monkeys using a simple reaction-time procedure. The threshold contour was a smooth W-shaped function with rises at the extreme frequencies and around 4 kHz and was comparable in shape with previously reported thresholds for this animal. Standard deviations a...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(78)90008-4
更新日期：1978-10-01 00:00:00
abstract：:The distribution of neurotensin-containing cell bodies and fibers has been observed in the central and peripheral nervous system, including sensory ganglia, but no description has been found in the peripheral auditory system. Here, we investigated the presence of neurotensin immunoreactivity in the cochlea of the adul...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2005.02.011
更新日期：2005-07-01 00:00:00
abstract：:One role of the inferior colliculus (IC) in bats is to create neuronal delay-tuning, which is used for the estimation of target distance in the echolocating bat's auditory system. In this study, we describe response properties of IC delay-tuned neurons of the mustached bat (Pteronotus parnellii) and compare it with th...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2012.02.013
更新日期：2012-04-01 00:00:00
abstract：:Hair cells of Sprague-Dawley rats aged 2-33 months were counted in order to assess the magnitude, location and time course of cell degeneration. The mean number of hair cell places (hair cells plus phalangeal scars) was approximately 4700: 960 inner hair cell places and 3470 outer hair cell places. These numbers do no...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(82)90017-x
更新日期：1982-12-01 00:00:00
abstract：:Hair cells of the lateral line system in fish may differ in their susceptibility to damage by aminoglycoside antibiotics. Gentamicin has been reported to damage hair cells within canal neuromasts, but not those within superficial neuromasts. This finding, based on SEM imaging, indicates a distinction in the physiology...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2010.01.001
更新日期：2010-03-01 00:00:00
abstract：:Recent measurements of vibrational motion to assess sound transmission through ear structures and skull contents have included three-dimensional (3D) behavior. The 3D motion of a point has been described with the three orthogonal components in the 3D space. In this article, a method to represent the 3D velocity with t...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2018.03.022
更新日期：2018-07-01 00:00:00
abstract：:The endocochlear potential and Na+ activity in the endolymph were simultaneously measured with a double-barreled Na+ selective liquid membrane electrode. The modified Na+ conductance and permeability coefficient of the endolymph-perilymph barrier were calculated from the rate of change in the endolymph Na+ concentrati...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(84)90045-5
更新日期：1984-08-01 00:00:00
abstract：:Carbohydrate distribution in the fresh utricular macula of the guinea pig was analysed using lectins such as Concanavalin A (ConA), Dolichos biflorus agglutinin (DBA), peanut agglutinin (PNA), soybean agglutinin (SBA), Ulex europeus agglutinin (UEA-1) and wheat germ agglutinin (WGA) by means of confocal laser scanning...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(95)00075-f
更新日期：1995-07-01 00:00:00
abstract：:Efferent neurones within the intraganglionic spiral bundle of the guinea pig cochlea were characterized in terms of their response properties, and their pattern of termination within the receptor organ revealed by intracellular labelling with horseradish peroxidase. All neurones subsequently identified as efferent neu...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(85)90059-0
更新日期：1985-01-01 00:00:00
abstract：:Kanamycin (KM)-induced changes in expression of the gene for glutamate-aspartate transporter (GLAST) in the rat cochlea were analyzed by Northern blotting. With the administration of KM (600 mg/kg/day) once daily for 20 days, the expression of GLAST mRNA gradually increased and reached a peak on day 20. Although the e...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(99)00050-7
更新日期：1999-07-01 00:00:00
abstract：:The inferior colliculus occupies a central position in ascending and descending auditory pathways. A substantial proportion of its neurons are GABAergic, and these neurons contribute to intracollicular circuits as well as to extrinsic projections to numerous targets. A variety of types of evidence - morphology, physio...
journal_title：Hearing research
pub_type： 杂志文章,评审
doi：10.1016/j.heares.2018.10.001
更新日期：2019-05-01 00:00:00
abstract：:Tinnitus is most often initiated by modality specific otopathologic disturbances affecting peripheral and central auditory pathways. However, there is growing evidence indicating that the anatomical location generating tinnitus occurs at sites different from the initial pathology. Support for this notion is found in i...
journal_title：Hearing research
pub_type： 杂志文章,评审
doi：10.1016/s0378-5955(02)00717-7
更新日期：2003-01-01 00:00:00
