Abstract:
PURPOSE:The Cre-loxP system has become an important strategy for conditional gene deletion and conditional gene expression in genetically engineered mice. To evaluate Cre recombinase expression, we generated reporter mice that permit both noninvasive imaging in living animals and either ex vivo histochemical/immunohistochemical tissue transgene expression analysis or quantitative enzyme analysis in the same animal. PROCEDURES:Transgenic reporter mice were generated in which a loxP-flanked enhanced green fluorescent protein (EGFP) reporter gene and STOP sequence are placed after the nearly ubiquitously expressed CAG promoter, but before a bicistronic transcriptional unit containing luciferase and β-galactosidase reporter gene coding sequences. RESULTS:After global deletion of the floxed STOP sequence by germ line Cre deletion, the reporter mouse expresses luciferase and β-galactosidase in all tissues examined. Tissue-specific expression of both reporter genes occurs in reporter mouse strains expressing Cre in skin (K14 keratin Cre), heart (myosin light chair Cre), or colon (Villin Cre). CONCLUSION:The luc-gal(Tg) reporter mouse allows noninvasive imaging of target Cre activation both in living animals and in tissues and cells following necropsy, using loss of EGFP expression, gain of luciferase expression, and gain of β-galactosidase expression as alternatives within the same animal for qualitative analysis of Cre expression.
journal_name
Mol Imaging Bioljournal_title
Molecular imaging and biologyauthors
Ishikawa TO,Herschman HRdoi
10.1007/s11307-010-0333-xsubject
Has Abstractpub_date
2011-04-01 00:00:00pages
284-92issue
2eissn
1536-1632issn
1860-2002journal_volume
13pub_type
杂志文章abstract:PURPOSE:Angiogenesis is essential for physiological processes as well as for carcinogenesis. New approaches to cancer therapy include targeting angiogenesis. One target is VEGF-A and its receptor VEGFR2. In this study, we sought to investigate pancreatic cancer angiogenesis in a genetically modified VEGFR2-luc-KI mouse...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-010-0310-4
更新日期:2010-12-01 00:00:00
abstract:INTRODUCTION:Technetium (Tc) methylene diphosphonate (MDP) has been the standard method for bone scintigraphy for three decades. (18)F sodium fluoride ((18)F NaF) positron emission tomography (PET)/computed tomography (CT) has better resolution and is considered superior. The role of 2-deoxy-2-[(18)F]fluoro-D-glucose (...
journal_title:Molecular imaging and biology
pub_type: 临床试验,杂志文章
doi:10.1007/s11307-011-0486-2
更新日期:2012-04-01 00:00:00
abstract:PURPOSE:We examine the feasibility of fluorescent imaging system for sentinel lymph node detection by using functionalized silica nanoparticles. MATERIALS AND METHODS:We developed a functionalized RITC-SiO(2) nanoparticles containing fluorescent dye, C(28)H(31)N(2)O(3)Cl (rhodamine B isothiocyanate) inside, and subseq...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-009-0262-8
更新日期:2010-04-01 00:00:00
abstract:PURPOSE:To determine the prevalence of viable myocardium in patients with ischemic cardiomyopathy and, to evaluate the value of three-dimensional (3-D) fusion imaging of myocardial scintigraphic and angiographic data to assign coronary artery lesions to the corresponding viable and nonviable myocardial territory. PROC...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1016/j.mibio.2004.02.002
更新日期:2004-05-01 00:00:00
abstract:PURPOSE:CXCR4 is overexpressed on tumor cells from many types of human cancers. A high level of CXCR4 expression often correlates with poor prognosis, chemotherapy resistance, and metastasis. The development of CXCR4-specific radiotracers for positron emission tomography (PET) imaging will allow in vivo evaluation of r...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-013-0640-0
更新日期:2013-12-01 00:00:00
abstract:PURPOSE:This study was undertaken to determine if artifacts from misalignment of cardiac emission to transmission data is present in adenosine stress studies and if the artifact could be reproduced by intentional misalignment in normal exams. PROCEDURES:Seventy consecutive 82Rb myocardial perfusion studies were review...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-008-0139-2
更新日期:2008-07-01 00:00:00
abstract:PURPOSE:[123I]FP-CIT (DaTSCAN®) single-photon emission computed tomography (SPECT) imaging is widely used to study neurodegenerative parkinsonism, by measuring presynaptic dopamine transporter (DAT) in striatal regions. Beyond DAT, [123I]FP-CIT may be considered for other monoaminergic systems, in particular the seroto...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-017-1052-3
更新日期:2017-10-01 00:00:00
abstract:PURPOSE:The demand to optimize multidisciplinary treatment strategies in patients with benign and malignant diseases of the lung and other organs has led to the increased need of mechanistic proof-of-concept studies in preclinical small animal models using new non-invasive imaging methods. Therefore, we evaluated the r...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-015-0890-0
更新日期:2016-04-01 00:00:00
abstract:PURPOSE:Prostate-specific membrane antigen (PSMA) continues to be the hallmark biomarker for prostate cancer as it is expressed on nearly all prostatic tumors. In addition, increased PSMA expression correlates with castration resistance and progression to the metastatic stage of the disease. Recently, we combined both ...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-019-01404-8
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVES:Accurate staging of patients with melanoma is vital to guide appropriate treatment. 2-Deoxy-2-[F-18]fluoro-D-glucose (FDG)-positron emission tomography (PET) has been reported to be a sensitive and specific technique for the staging of advanced melanoma, however, few studies provide information regarding its...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-005-0002-7
更新日期:2005-07-01 00:00:00
abstract:PURPOSE:The vesicular acetylcholine transporter (VAChT) is a specific biomarker for imaging presynaptic cholinergic neurons. The syntheses and C-11 labeling of two potent enantiopure VAChT inhibitors are reported here. PROCEDURES:Two VAChT inhibitors, (±)-2 and (±)-6, were successfully synthesized. A chiral HPLC colum...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-014-0748-x
更新日期:2014-12-01 00:00:00
abstract:PURPOSE:The present work was aimed at the development of prospective positron emission tomography (PET) agents for infection imaging. Towards this aim, ubiquicidin (UBI) fragments conjugated with the macrocyclic NODAGA chelator were radiolabeled with Ga-68 and evaluated. PROCEDURES:Conformations of custom synthesized ...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-016-0983-4
更新日期:2017-02-01 00:00:00
abstract:PURPOSE:The A1AR antagonist 8-cyclopentyl-3-(3-fluoropropyl)-1-propylxanthine ([(18)F]CPFPX) has recently been shown to be a suitable radiotracer for quantitative in vivo imaging of the A1 adenosine receptor (A1AR) in rats. The present study evaluates the reproducibility of non-invasive longitudinal A1AR studies with [...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-014-0729-0
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:[18F]Mefway is a positron emission tomography (PET) radioligand for quantification of the brain serotonin 1A (5-HT1A) receptor density. The purpose of this study was to evaluate the radiation safety of [18F]Mefway in humans. PROCEDURES:Six healthy volunteers (three males and three females) were whole-body PET ...
journal_title:Molecular imaging and biology
pub_type: 临床试验,杂志文章
doi:10.1007/s11307-016-0955-8
更新日期:2016-12-01 00:00:00
abstract:PURPOSE:Some patients cannot remain immobile for a long duration of 60 min, which is generally applied in the case of a 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) dynamic positron emission tomography (PET) scan. We investigated the change of the parametric values when the time duration of PET data was shortened. P...
journal_title:Molecular imaging and biology
pub_type: 临床试验,杂志文章
doi:10.1007/s11307-010-0384-z
更新日期:2011-08-01 00:00:00
abstract:PURPOSE:This study aims to develop a molecular imaging strategy for response assessment of arginine deiminase (ADI) treatment in melanoma xenografts using 3'-[(18)F]fluoro-3'-deoxythymidine ([(18)F]-FLT) positron emission tomography (PET). PROCEDURES:F-FLT response to ADI therapy was studied in preclinical models of m...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-013-0655-6
更新日期:2013-12-01 00:00:00
abstract:PURPOSE:Positron emission tomography (PET) with 2-deoxy-2-[18F]fluoro-D-glucose (FDG) has been used for various cancers, but reproducibility of common utilized semi-quantitative parameters, such as the maximal single pixel standardized uptake value (SUV) and effective glycolytic volume (EGV), remains unknown. Knowledge...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1016/s1536-1632(01)00004-x
更新日期:2002-03-01 00:00:00
abstract:PURPOSE:The purpose of this study was to assess the value of the spatial heterogeneity of somatostatin receptor (SSR) volume, quantified as asphericity (ASP), and to predict response to peptide receptor radionuclide therapy (PRRT) in patients with metastatic gastroenteropancreatic neuroendocrine neoplasms (GEP-NEN). P...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-016-1018-x
更新日期:2017-06-01 00:00:00
abstract:PURPOSE:Noninvasive imaging of cell necrosis can provide an early evaluation of tumor response to treatments. Here, we aimed to design and synthesize a novel diindole-based magnetic resonance imaging (MRI) contrast agent (Gd-bis-DOTA-diindolylmethane, Gd-DIM) for assessment of tumor response to therapy at an early stag...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-019-01399-2
更新日期:2020-06-01 00:00:00
abstract::2'-deoxy-2'-[(18)F]fluoro-5-ethyl-1-beta-D-arabinofuranosyluracil ([(18)F]FEAU) is a promising radiolabeled nucleoside designed to monitor Herpes Simplex Virus Type 1 thymidine kinase (HSV1-tk) reporter gene expression with positron emission tomography (PET). However, the challenging radiosynthesis creates problems fo...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-007-0122-3
更新日期:2008-03-01 00:00:00
abstract:PURPOSE:The transplantation of pancreatic islets is a promising cell replacement therapy for type 1 diabetes. Subcutaneous islet transplantation is currently under investigation as a means to circumvent problems associated with standard intra-hepatic islet transplantation. As modifications are being developed to improv...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-020-01560-2
更新日期:2020-11-02 00:00:00
abstract::Positron emission tomography (PET) with epidermal growth factor receptor (EGFR) kinase-specific radiolabeled tracers could provide the means for noninvasive and repetitive imaging of heterogeneity of EGFR expression and signaling activity in tumors in individual patients before and during therapy with EGFR signaling i...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-006-0049-0
更新日期:2006-09-01 00:00:00
abstract:PURPOSE:Although current therapies for many inflammatory/autoimmune diseases are effective, a significant number of patients still exhibit only partial or negligible responses to therapeutic intervention. Since prolonged use of an inadequate therapy can result in both progressive tissue damage and unnecessary expense, ...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-015-0876-y
更新日期:2016-04-01 00:00:00
abstract:INTRODUCTION:Advances in positron emission tomography (PET) imaging have provided opportunities to develop radiotracers specific for imaging insulin-producing pancreatic β-cells. However, a host of lingering questions should be addressed before these radiotracers are advocated for noninvasive quantification of β-cell m...
journal_title:Molecular imaging and biology
pub_type: 杂志文章,评审
doi:10.1007/s11307-013-0620-4
更新日期:2013-04-01 00:00:00
abstract:PURPOSE:[(18)F]Flumazenil, which has the advantage of a longer half-life than [(11)C]flumazenil, is well known for determining of the central benzodiazepine receptor concentrations. However, [(18)F]flumazenil has not been widely used because fluctuating and relatively low yields render automatic production insufficient...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-014-0738-z
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:Rats are important preclinical models for studying breast cancer metastasis and bone pathologies. In these research areas, fluorescence molecular tomography (FMT) is commonly applied for quantitative three-dimensional (3D) imaging in mice. However, uncertainties due to strong depth dependency of FMT signal and ...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-013-0698-8
更新日期:2014-06-01 00:00:00
abstract:PURPOSE:Diabetes is associated with a deterioration of the microvasculature in brown adipose tissue (BAT) and with a decrease in its metabolic activity. Multispectral optoacoustic tomography has been recently proposed as a new tool capable of differentiating healthy and diabetic BAT by observing hemoglobin gradients an...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-018-1291-y
更新日期:2019-08-01 00:00:00
abstract:PURPOSE:The purpose of the study is to synthesize and characterize near-infrared (NIR) fluorescence imaging probes targeted to gelatinases. PROCEDURES:A phage display-selected cyclic peptide containing the His-Try-Gly-Phe (HWGF) motif was used as the lead compound. Structure-activity relationship analysis was used to ...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-009-0219-y
更新日期:2009-11-01 00:00:00
abstract:PURPOSE:Manganese ion has been extensively used as a magnetic resonance imaging (MRI) contrast agent in preclinical studies to assess tissue anatomy, function, and neuronal connectivity. Unfortunately, its use in human studies has been limited by cellular toxicity and the need to use a very low dose. The much higher se...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-018-1162-6
更新日期:2018-08-01 00:00:00
abstract:PURPOSE:Inflammation plays a key role in the progression of intervertebral disk (IVD) herniation and associated low back pain. However, real-time spatial diagnosis of inflammation associated with acute disk herniation has not been investigated. We sought to detect local neutrophil and macrophage infiltration near disk ...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-016-1038-6
更新日期:2017-10-01 00:00:00