Validation of fluorescence molecular tomography/micro-CT multimodal imaging in vivo in rats.

Abstract:

PURPOSE:Rats are important preclinical models for studying breast cancer metastasis and bone pathologies. In these research areas, fluorescence molecular tomography (FMT) is commonly applied for quantitative three-dimensional (3D) imaging in mice. However, uncertainties due to strong depth dependency of FMT signal and spatial resolution require a validation study in rats. PROCEDURE:FMT performance in rats was assessed based on co-registered FMT/micro-computed tomography (micro-CT) reconstructed volumes obtained from optical phantoms and from models relevant for tumor imaging, bone remodeling and biodistribution analysis of nanoparticles. RESULTS:FMT reconstructions within 20-mm-thick optical phantoms were accurate (95 ± 11 % recovery), precise (CV ≤ 8 %) and linear (R(2) > 0.9788) over a range of 78-2,500 nM of the near infrared fluorescent agent VivoTag 750 (VT(750)). In vivo, implanted defined fluorescent targets yielded a recovery of 105 ± 5 % and successfully co-registered with micro-CT delineated structures. Additionally, using the bone-targeting imaging agent Osteosense 750, regions of neo bone formation identified by FMT could be mapped to the region of epiphyseal growth plates observed in micro-CT images. Finally, as a proof of concept, to monitor nanoparticulate drug pharmacokinetics in rat subjects the accumulation/clearance of VT(750)-albumin conjugate in/from the liver was followed at 11 different time points over a period of 2 weeks by FMT/micro-CT. CONCLUSIONS:FMT imaging has been validated in optical phantoms as well as in 160 g rats, and sequential FMT/micro-CT imaging can be considered as a useful tool for preclinical research in rats.

journal_name

Mol Imaging Biol

authors

Vonwil D,Christensen J,Fischer S,Ronneberger O,Shastri VP

doi

10.1007/s11307-013-0698-8

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

350-61

issue

3

eissn

1536-1632

issn

1860-2002

journal_volume

16

pub_type

杂志文章
  • Impact of FDG-PET on prediction of clinical outcome after concurrent chemoradiotherapy in hypopharyngeal carcinoma.

    abstract:PURPOSE:To evaluate prognostic value of pretreatment and posttreatment 2-deoxy-2-[18F]fluoro-D-glucose positron emission tomography (FDG-PET) in advanced hypopharyngeal carcinoma treated by chemoradiotherapy. PROCEDURES:Thirty-one patients underwent a baseline FDG-PET and then FDG-PET was repeated 7 weeks after chemor...

    journal_title:Molecular imaging and biology

    pub_type: 临床试验,杂志文章

    doi:10.1007/s11307-009-0229-9

    authors: Inohara H,Enomoto K,Tomiyama Y,Higuchi I,Inoue T,Hatazawa J

    更新日期:2010-01-01 00:00:00

  • Imaging of gadolinium spatial distribution in tumor tissue by laser ablation inductively coupled plasma mass spectrometry.

    abstract:PURPOSE:Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was utilized in postmortem imaging of gadolinium (Gd) spatial distribution in a mouse tumor model postadministration of PEGylated Gd liposomal nanoparticles. PROCEDURES:PEGylated liposomal nanoparticles were formulated using a paramagnetic...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-009-0282-4

    authors: Kamaly N,Pugh JA,Kalber TL,Bunch J,Miller AD,McLeod CW,Bell JD

    更新日期:2010-08-01 00:00:00

  • Semiquantitative Parameters in PSMA-Targeted PET Imaging with [18F]DCFPyL: Impact of Tumor Burden on Normal Organ Uptake.

    abstract:PURPOSE:In this study, we aimed to quantitatively investigate the biodistribution of [18F]DCFPyL in patients with prostate cancer (PCa) and to determine whether uptake in normal organs correlates with an increase in tumor burden. PROCEDURES:Fifty patients who had been imaged with [18F]DCFPyL positron emission tomograp...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-019-01375-w

    authors: Werner RA,Bundschuh RA,Bundschuh L,Lapa C,Yin Y,Javadi MS,Buck AK,Higuchi T,Pienta KJ,Pomper MG,Lodge MA,Gorin MA,Rowe SP

    更新日期:2020-02-01 00:00:00

  • Quantitating antibody uptake in vivo: conditional dependence on antigen expression levels.

    abstract:PURPOSE:Antibodies form an important class of cancer therapeutics, and there is intense interest in using them for imaging applications in diagnosis and monitoring of cancer treatment. Despite the expanding body of knowledge describing pharmacokinetic and pharmacodynamic interactions of antibodies in vivo, discrepancie...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-010-0397-7

    authors: Thurber GM,Weissleder R

    更新日期:2011-08-01 00:00:00

  • Sodium [¹⁸F]fluoride PET/CT in myocardial infarction.

    abstract:PURPOSE:Sodium [(18)F]fluoride (Na[(18)F]F) positron emission tomography with integrated computed tomography (PET/CT) has not been used for imaging myocardial infarction (MI). Here, we aimed to investigate the Na[(18)F]F PET/CT features of MI in a rat model. PROCEDURES:MI was induced by coronary artery ligation in 8-w...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-014-0796-2

    authors: Han JH,Lim SY,Lee MS,Lee WW

    更新日期:2015-04-01 00:00:00

  • Early assessment of therapeutic response using FDG PET in small cell lung cancer.

    abstract:PURPOSE:We evaluated the ability of 2-deoxy-2-(18)F-fluoro-D-glucose (FDG) positron emission tomography (PET) in the early assessment of therapeutic response in patients with small cell lung cancer (SCLC). PROCEDURES:FDG PET studies were performed before (baseline PET), after the first cycle of chemotherapy (early PET...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-009-0227-y

    authors: Yamamoto Y,Kameyama R,Murota M,Bandoh S,Ishii T,Nishiyama Y

    更新日期:2009-11-01 00:00:00

  • To Explore a Representative Hypoxic Parameter to Predict the Treatment Response and Prognosis Obtained by [18F]FMISO-PET in Patients with Non-small Cell Lung Cancer.

    abstract:PURPOSE:To explore a representative hypoxic parameter to predict the treatment response and prognosis for [18F]fluoromisonidazole ([18F]FMISO) positron emission tomography (PET)/X-ray computed tomography (CT) in patients with non-small cell lung cancer (NSCLC). PROCEDURES:Twenty-nine patients with NSCLC underwent FMIS...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-018-1190-2

    authors: Li L,Wei Y,Huang Y,Yu Q,Liu W,Zhao S,Zheng J,Lu H,Yu J,Yuan S

    更新日期:2018-12-01 00:00:00

  • 2-Deoxy-2-[18F]fluoro-D-glucose-positron emission tomography and positron emission tomography/computed tomography diagnosis of patients with recurrent papillary thyroid cancer.

    abstract:OBJECTIVE:2-Deoxy-2-[F-18]fluoro-D-glucose positron emission tomography (FDG-PET) has an established role in restaging of various cancers, including papillary and undifferentiated thyroid carcinoma, but detection rates are variable in the published literature. We were therefore prompted to review our experience with FD...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-006-0046-3

    authors: Iagaru A,Masamed R,Singer PA,Conti PS

    更新日期:2006-09-01 00:00:00

  • A comparison between time domain and spectral imaging systems for imaging quantum dots in small living animals.

    abstract:PURPOSE:We quantified the performance of time-domain imaging (TDI) and spectral imaging (SI) for fluorescence imaging of quantum dots (QDs) in three distinct imaging instruments: eXplore Optix (TDI, Advanced Research Technologies Inc.), Maestro (SI, CRi Inc.), and IVIS-Spectrum (SI, Caliper Life Sciences Inc.). PROCED...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-009-0290-4

    authors: de la Zerda A,Bodapati S,Teed R,Schipper ML,Keren S,Smith BR,Ng JS,Gambhir SS

    更新日期:2010-10-01 00:00:00

  • Use of positron emission tomography to target prostate cancer gene therapy by oncolytic herpes simplex virus.

    abstract::Herpes simplex virus (HSV) oncolytic gene therapy is a promising treatment modality against cancer. We have demonstrated that androgen-induced cellular changes enhance oncolytic viral replication and improve efficacy in the treatment of androgen-dependent prostate cancer cell line. Imaging of changes in 2-deoxy-2-[F-1...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-005-0028-x

    authors: Mullerad M,Eisenberg DP,Akhurst TJ,Adusumilli PS,Riedl CC,Bhargava A,Gonen M,Finn R,Scardino PT,Fong Y

    更新日期:2006-01-01 00:00:00

  • MicroPET imaging of integrin αvβ3 expressing tumors using 89Zr-RGD peptides.

    abstract:PURPOSE:The dimeric transmembrane integrin, α(v)β(3), is a well-investigated target by different imaging modalities through suitably labeled arginine-glycine-aspartic acid (RGD) containing peptides. In this study, we labeled four cyclic RGD peptides with or without PEG functional groups: c(RGDfK) (denoted as FK), PEG(3...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-010-0458-y

    authors: Jacobson O,Zhu L,Niu G,Weiss ID,Szajek LP,Ma Y,Sun X,Yan Y,Kiesewetter DO,Liu S,Chen X

    更新日期:2011-12-01 00:00:00

  • (2S,4R)-4-[18F]Fluoroglutamine as a PET Indicator for Bone Marrow Metabolism Dysfunctional: from Animal Experiments to Clinical Application.

    abstract:PURPOSE:Previous reports confirmed that (2S,4R)-4-[18F]Fluoroglutamine ([18F]GLN) accumulated in bone and bone marrow. This study investigates the potential of using [18F]GLN positron emission tomography (PET) to monitor changes of bone marrow activity after chemotherapy (myelosuppression). PROCEDURES:Bone marrow inhi...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-019-01319-4

    authors: Zhu H,Liu F,Zhang Y,Yang J,Xu X,Guo X,Liu T,Li N,Zhu L,Kung HF,Yang Z

    更新日期:2019-10-01 00:00:00

  • Immunoglobulin G (IgG)-Based Imaging Probe Accumulates in M1 Macrophage-Infiltrated Atherosclerotic Plaques Independent of IgG Target Molecule Expression.

    abstract:PURPOSE:Vulnerable plaques are key factors for ischemic diseases. Thus, their precise detection is necessary for the diagnosis of such diseases. Immunoglobulin G (IgG)-based imaging probes have been developed for imaging biomolecules related to plaque formation for the diagnosis of atherosclerosis. However, IgG accumul...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-016-1036-8

    authors: Shimizu Y,Hanzawa H,Zhao Y,Fukura S,Nishijima KI,Sakamoto T,Zhao S,Tamaki N,Ogawa M,Kuge Y

    更新日期:2017-08-01 00:00:00

  • Reproducibility of Contrast-Enhanced Ultrasound in Mice with Controlled Injection.

    abstract:PURPOSE:Sensitivity of contrast-enhanced ultrasound (CEUS) to microvascular flow modifications can be limited by intra-injection variability (injected dose, rate, volume). PROCEDURES:To evaluate the effect of injection variability on microvascular flow evaluation, CEUS was compared between controlled and manual inject...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-016-0952-y

    authors: Dizeux A,Payen T,Barrois G,Le Guillou Buffello D,Bridal SL

    更新日期:2016-10-01 00:00:00

  • Comparison of [¹¹C]choline ([¹¹C]CHO) and S(+)-β-methyl-[¹¹C]choline ([¹¹C]SMC) as imaging probes for prostate cancer in a PC-3 prostate cancer xenograft model.

    abstract:PURPOSE:Carbon-11- and fluorine-18-labeled choline derivatives have been introduced as promising tracers for prostate cancer imaging. However, due to limited specificity and sensitivity, there is a need for new tracers with higher sensitivity and specificity for diagnosing prostate cancer to improve tracer uptake and e...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-014-0782-8

    authors: Schwarzenböck SM,Gertz J,Souvatzoglou M,Kurth J,Sachs D,Nawroth R,Treiber U,Schuster T,Senekowitsch-Schmidtke R,Schwaiger M,Ziegler SI,Henriksen G,Wester HJ,Krause BJ

    更新日期:2015-04-01 00:00:00

  • Syntheses and radiosyntheses of two carbon-11 labeled potent and selective radioligands for imaging vesicular acetylcholine transporter.

    abstract:PURPOSE:The vesicular acetylcholine transporter (VAChT) is a specific biomarker for imaging presynaptic cholinergic neurons. The syntheses and C-11 labeling of two potent enantiopure VAChT inhibitors are reported here. PROCEDURES:Two VAChT inhibitors, (±)-2 and (±)-6, were successfully synthesized. A chiral HPLC colum...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-014-0748-x

    authors: Padakanti PK,Zhang X,Li J,Parsons SM,Perlmutter JS,Tu Z

    更新日期:2014-12-01 00:00:00

  • Cancer Patient-Derived Circulating Microparticles Enhance Lung Metastasis in a Rat Model: Dual-Source CT, Cellular, and Molecular Studies.

    abstract:PURPOSE:This study aimed to test the hypothesis that lung cancer patient-derived circulating microparticles (LCC-MPs) enhance metastatic lung tumors in a rat model. PROCEDURES:The controls (n = 6) and LCC-MP-treated rats (n = 6) with N1S1-induced pulmonary metastatic hepatocellular carcinoma (HCC) underwent dual-sourc...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-015-0923-8

    authors: Ko SF,Yip HK,Zhen YY,Lee CC,Li JH,Lee CC,Leu S,Huang CC,Ng SH,Lin JW

    更新日期:2016-08-01 00:00:00

  • Bioluminescence imaging of angiogenesis in a murine orthotopic pancreatic cancer model.

    abstract:PURPOSE:Angiogenesis is essential for physiological processes as well as for carcinogenesis. New approaches to cancer therapy include targeting angiogenesis. One target is VEGF-A and its receptor VEGFR2. In this study, we sought to investigate pancreatic cancer angiogenesis in a genetically modified VEGFR2-luc-KI mouse...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-010-0310-4

    authors: Angst E,Chen M,Mojadidi M,Hines OJ,Reber HA,Eibl G

    更新日期:2010-12-01 00:00:00

  • MRI of ICAM-1 upregulation after stroke: the importance of choosing the appropriate target-specific particulate contrast agent.

    abstract:PURPOSE:Magnetic resonance imaging (MRI) with targeted contrast agents provides a promising means for diagnosis and treatment monitoring after cerebrovascular injury. Our goal was to demonstrate the feasibility of this approach to detect the neuroinflammatory biomarker intercellular adhesion molecule-1 (ICAM-1) after s...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-013-0617-z

    authors: Deddens LH,van Tilborg GA,van der Toorn A,van der Marel K,Paulis LE,van Bloois L,Storm G,Strijkers GJ,Mulder WJ,de Vries HE,Dijkhuizen RM

    更新日期:2013-08-01 00:00:00

  • MicroCT liver contrast agent enhancement over time, dose, and mouse strain.

    abstract:PURPOSE:The aim of this study was to examine strain differences in the uptake of Fenestra liver contrast agent (LC) in Nude, C57, and severe-combined immunodeficient (SCID) mice. In addition, we aimed to determine optimum dosing and to determine if there are positron emission tomography (PET)-attenuation effects on 2-d...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-007-0128-x

    authors: Suckow CE,Stout DB

    更新日期:2008-03-01 00:00:00

  • Axl-targeted cancer imaging with humanized antibody h173.

    abstract:PURPOSE:The tyrosine kinase receptor Axl is overexpressed in various types of cancer and correlated with cancer malignancy. Selective Axl blockade reduces tumor growth and metastasis. The purpose of this study was to examine whether the humanized anti-Axl antibody humanized 173 (h173) labeled with near-infrared fluores...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-013-0714-z

    authors: Li D,Liu S,Liu R,Park R,Yu H,Krasnoperov V,Gill PS,Li Z,Shan H,Conti PS

    更新日期:2014-08-01 00:00:00

  • Anatomy, Functionality, and Neuronal Connectivity with Manganese Radiotracers for Positron Emission Tomography.

    abstract:PURPOSE:Manganese ion has been extensively used as a magnetic resonance imaging (MRI) contrast agent in preclinical studies to assess tissue anatomy, function, and neuronal connectivity. Unfortunately, its use in human studies has been limited by cellular toxicity and the need to use a very low dose. The much higher se...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-018-1162-6

    authors: Saar G,Millo CM,Szajek LP,Bacon J,Herscovitch P,Koretsky AP

    更新日期:2018-08-01 00:00:00

  • Whole body biodistribution and radiation dosimetry in humans of a new PET ligand, [(18)F]-FEPPA, to image translocator protein (18 kDa).

    abstract:PURPOSE:[(18)F]-FEPPA is a translocator protein (18 kDa, TSPO) positron emission tomography (PET) radiotracer. Radiation dosimetry was estimated from the whole body biodistribution, taking into consideration TSPO rs6971 (Ala147Thr) polymorphism. PROCEDURES:[(18)F]-FEPPA whole body PET scans were acquired for six healt...

    journal_title:Molecular imaging and biology

    pub_type: 临床试验,杂志文章

    doi:10.1007/s11307-012-0589-4

    authors: Mizrahi R,Rusjan PM,Vitcu I,Ng A,Wilson AA,Houle S,Bloomfield PM

    更新日期:2013-06-01 00:00:00

  • Integrin α(v)β₃ as a PET imaging biomarker for osteoclast number in mouse models of negative and positive osteoclast regulation.

    abstract:PURPOSE:The goal of this study was to determine the specificity of ⁶⁴Cu-CB-TE2A-c(RGDyK) (⁶⁴Cu-RGD) for osteoclast-related diseases, such as Paget's disease or rheumatoid arthritis. PROCEDURES:C57BL/6 mice were treated systemically with osteoprotegerin (OPG) for 15 days or RANKL for 11 days to suppress and stimulate o...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-011-0512-4

    authors: Zheleznyak A,Wadas TJ,Sherman CD,Wilson JM,Kostenuik PJ,Weilbaecher KN,Anderson CJ

    更新日期:2012-08-01 00:00:00

  • Correction to: Differentiation of Malignant and Benign Head and Neck Tumors with Amide Proton Transfer-Weighted MR Imaging.

    abstract::This article is about head and neck tumor MR imaging and is not within the scope of two National Institutes of Health grants (R0ICA166171, ROICA228188) erroneously listed. Thus, the authors would like to remove the reference to the NIH grants R01CA166171 and R01CA228188 in this article. ...

    journal_title:Molecular imaging and biology

    pub_type: 已发布勘误

    doi:10.1007/s11307-019-01459-7

    authors: Yu L,Li C,Luo X,Zhou J,Zhang C,Zhang Y,Chen M

    更新日期:2020-02-01 00:00:00

  • Histogram Analysis of T1-Weighted, T2-Weighted, and Postcontrast T1-Weighted Images in Primary CNS Lymphoma: Correlations with Histopathological Findings-a Preliminary Study.

    abstract:PURPOSE:Previously, some reports mentioned that magnetic resonance imaging (MRI) can predict histopathological features in primary CNS lymphoma (PCNSL). The reported data analyzed diffusion-weighted imaging findings. The aim of this study was to investigate possible associations between histopathological findings, such...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-017-1115-5

    authors: Meyer HJ,Schob S,Münch B,Frydrychowicz C,Garnov N,Quäschling U,Hoffmann KT,Surov A

    更新日期:2018-04-01 00:00:00

  • PET Imaging of HER2-Positive Tumors with Cu-64-Labeled Affibody Molecules.

    abstract:PURPOSE:Previous studies has demonstrated the utility of human epidermal growth factor receptor type 2 (HER2) as an attractive target for cancer molecular imaging and therapy. An affibody protein with strong binding affinity for HER2, ZHER2:342, has been reported. Various methods of chelator conjugation for radiolabeli...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-018-01310-5

    authors: Qi S,Hoppmann S,Xu Y,Cheng Z

    更新日期:2019-10-01 00:00:00

  • Evaluation of [99mTc]Radiolabeled Macrophage Mannose Receptor-Specific Nanobodies for Targeting of Atherosclerotic Lesions in Mice.

    abstract:PURPOSE:Macrophage accumulation characterizes the development of atherosclerotic plaques, and the presence of certain macrophage subsets might be an indicator of plaque phenotype and (in)stability. The macrophage mannose receptor (MMR) is expressed on alternatively activated macrophages and found at sites of intraplaqu...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-017-1117-3

    authors: Bala G,Baudhuin H,Remory I,Gillis K,Debie P,Krasniqi A,Lahoutte T,Raes G,Devoogdt N,Cosyns B,Hernot S

    更新日期:2018-04-01 00:00:00

  • Shortening the duration of [18F]FDG PET brain examination for diagnosis of brain glioma.

    abstract:PURPOSE:Some patients cannot remain immobile for a long duration of 60 min, which is generally applied in the case of a 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) dynamic positron emission tomography (PET) scan. We investigated the change of the parametric values when the time duration of PET data was shortened. P...

    journal_title:Molecular imaging and biology

    pub_type: 临床试验,杂志文章

    doi:10.1007/s11307-010-0384-z

    authors: Monden T,Kudomi N,Sasakawa Y,Yamamoto Y,Kawai N,Nishiyama Y

    更新日期:2011-08-01 00:00:00

  • In vivo imaging of sentinel nodes using fluorescent silica nanoparticles in living mice.

    abstract:PURPOSE:We examine the feasibility of fluorescent imaging system for sentinel lymph node detection by using functionalized silica nanoparticles. MATERIALS AND METHODS:We developed a functionalized RITC-SiO(2) nanoparticles containing fluorescent dye, C(28)H(31)N(2)O(3)Cl (rhodamine B isothiocyanate) inside, and subseq...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章

    doi:10.1007/s11307-009-0262-8

    authors: Jeon YH,Kim YH,Choi K,Piao JY,Quan B,Lee YS,Jeong JM,Chung JK,Lee DS,Lee MC,Lee J,Chung DS,Kang KW

    更新日期:2010-04-01 00:00:00