Abstract：
:Aminopeptidase H11, an integral membrane glycoprotein present only in the gut of Haemonchus contortus, could provide substantial protection as shown by 90% reduction in fecal egg counts, while its recombinant version expressed in E. coli induced little. To investigate the characteristics further, we amplified mRNA of H11 gene via reverse transcriptase polymerase chain reaction, followed by isolation of its 1,517-bp 5'-flanking region and determination of its genomic organization. The H11 gene contained 25 exons separated by 24 introns and spans 14,959 bp of genomic DNA. Analysis of the 1,517 bp 5'-flanking region of the H11 gene revealed a putative "TATA-less" promoter. Partial sequences of the last exon and its 3'-UTR of H11 isoform H11-4 were also identified upstream to the H11 gene with the same transcription orientation. The 1,517-bp 5'-flanking region and part of the first exon of the H11 gene were subcloned into the vector upstream of green fluorescence protein reporter gene and microinjected into the gonads of Caenorhabditis elegans. The transformed animals exhibited fluorescence in the distal intestine in the L4 larvae stage and adult worms. This study characterized gene structure of aminopeptidase H11, demonstrated different transcriptional pattern of its promoter region between free-living and blood-sucking nematode species, and highlights the utility of C. elegans as a heterologous system to study the biology roles of H11 isoforms.
journal_name
Funct Integr Genomicsjournal_title
Functional & integrative genomicsauthors
Zhou QJ,Zhang HL,Jiang XL,Du AFdoi
10.1007/s10142-010-0172-5subject
Has Abstractpub_date
2010-11-01 00:00:00pages
589-601issue
4eissn
1438-793Xissn
1438-7948journal_volume
10pub_type
杂志文章abstract：:Rhizoctonia solani AG1-IA is a soil-borne necrotrophic pathogen that causes devastating rice sheath blight disease in rice-growing regions worldwide. Sclerotia play an important role in the life cycle of R. solani AG1-IA. In this study, RNA sequencing was used to investigate the transcriptomic dynamics of sclerotial d...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-019-00677-0
更新日期：2019-09-01 00:00:00
abstract：:Control of proteolysis is important for plant growth, development, responses to stress, and defence against insects and pathogens. Members of the serpin protein family are likely to play a critical role in this control through irreversible inhibition of endogenous and exogenous target proteinases. Serpins have been fo...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-007-0059-2
更新日期：2008-02-01 00:00:00
abstract：:We have previously reported that the deletion of BMAL1 gene has opposite effects in respect to its contribution to the pathways that are effective in the multistage carcinogenesis process. BMAL1 deletion sensitized nearly normal breast epithelial (MCF10A) and invasive breast cancer cells (MDA-MB-231) to cisplatin- and...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-020-00757-6
更新日期：2021-01-01 00:00:00
abstract：:EST-derived microsatellites or simple sequence repeats (eSSR) occur in expressed sequence tags (EST). Here we report characteristics of eSSRs in the wheat genome, construction of consensus chromosome bin maps of SSR-containing ESTs ((SSR)ESTs), and development of eSSR markers for the 21 wheat chromosomes. A Perl scrip...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-004-0128-8
更新日期：2005-04-01 00:00:00
abstract：:Bread wheat (Triticum aestivum L.) is one of the most important crops globally and a high priority for genetic improvement, but its large and complex genome has been seen as intractable to whole genome sequencing. Isolation of individual wheat chromosome arms has facilitated large-scale sequence analyses. However, so ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-011-0250-3
更新日期：2012-03-01 00:00:00
abstract：:Advances in our understanding of genome structure provide consistent evidence for the existence of a core genome representing species classically defined by phenotype, as well as conditionally dispensable components of the genome that shows extensive variation between individuals of a given species. Generally, conserv...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-013-0319-2
更新日期：2013-03-01 00:00:00
abstract：:Aldo/keto reductases (AKRs) constitute a multitasking protein family that catalyzes diverse metabolic transformations including detoxification of stress generated reactive aldehydes. Yet this important protein family is poorly understood particularly in cyanobacteria, the ecologically most diverse and significant grou...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-017-0547-y
更新日期：2017-07-01 00:00:00
abstract：:Comparative genomic analysis is the cornerstone of in silico-based approaches to understanding biological systems and processes across cereal species, such as rice, wheat and barley, in order to identify genes of agronomic interest. The size of the genomic repositories is nearly doubling every year, and this has signi...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-004-0102-5
更新日期：2004-03-01 00:00:00
abstract：:MicroRNAs (miRNAs) play a pivotal role in post-transcriptional regulation of gene expression in plants. In this study, we investigate miRNAs in an agronomically important common tobacco in China, named Honghua Dajinyuan (a drought-tolerant cultivar). Here, we report a comprehensive analysis of miRNA expression profile...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0365-4
更新日期：2014-06-01 00:00:00
abstract：:Auxin response factors (ARFs) are recently discovered transcription factors that bind with auxin response elements (AuxRE, TGTCTC) to regulate the expression of early auxin-responsive genes. To our knowledge, the ARF gene family has never been characterized in cotton, the most important fiber crop in the world. In thi...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-015-0437-0
更新日期：2015-07-01 00:00:00
abstract：:The assembly of a 1.3-Mb size region of the wheat genome has provided the opportunity to study a recent nuclear mitochondrial DNA insertion (NUMT). In the present study, we have studied two bacterial artificial chromosomes (BACs) and characterized a 52-kb NUMT segment from the tetraploid and hexaploid wheat BAC librar...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-011-0237-0
更新日期：2011-12-01 00:00:00
abstract：:Natural selection drives species adaptations to biotic and abiotic stresses. Species distributed along a moisture gradient, such as Stipa purpurea, a dominant grass in alpine arid and semi-arid meadows on the Tibetan Plateau, provide an opportunity to evaluate the effects of long-term adaptation to differing degrees o...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0419-7
更新日期：2015-05-01 00:00:00
abstract：:Seasonal estrus is a key factor limiting animal fertility, and understanding the molecular mechanisms that regulate animal estrus is important for improving animal fertility. The pituitary gland, which is the most important endocrine gland in mammals, plays an important role in regulating the physiological processes s...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-020-00735-y
更新日期：2020-07-01 00:00:00
abstract：:Carefully analyzed expression profiles can serve as a valuable reference for deciphering gene functions. We exploited the potential of whole genome microarrays to measure the spatial and temporal expression profiles of rice genes in 19 stages of vegetative and reproductive development. We could verify expression of 22...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-012-0274-3
更新日期：2012-06-01 00:00:00
abstract：:Microarray experiments are being increasingly used in molecular biology. A common task is to detect genes with differential expression across two experimental conditions, such as two different tissues or the same tissue at two time points of biological development. To take proper account of statistical variability, so...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-002-0066-2
更新日期：2002-08-01 00:00:00
abstract：:The ATP-binding cassette (ABC) transporters are a large family of proteins responsible for the translocation of a variety of compounds across the membranes of both prokaryotes and eukaryotes. The inter-protein and intra-protein interactions in these traffic ATPases are still only poorly understood. In the present stud...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-001-0040-4
更新日期：2002-04-01 00:00:00
abstract：:The response of a grass halophyte Spartina alterniflora at early stages of salt stress was investigated through generation and systematic analysis of expressed sequence tags (ESTs) from both leaf and root tissues. Random EST sequencing produced 1,227 quality ESTs, which were clustered into 127 contigs, and 368 were si...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-008-0075-x
更新日期：2008-08-01 00:00:00
abstract：:Campylobacter jejuni remains a major cause of human gastroenteritis with estimated annual incidence rate of 450 million infections worldwide. C. jejuni is a major burden to public health in both socioeconomically developing and industrialized nations. Virulence determinants involved in C. jejuni pathogenesis are multi...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-016-0530-z
更新日期：2017-01-01 00:00:00
abstract：:As the amino acid storing organelle, the protein bodies provide nutrients for embryo development, seed germination and early seedling growth through storage proteolysis in cereal plants, such as wheat and rice. In protein bodies, the monomeric and polymeric prolamins, i.e. gliadins and glutenins, form gluten and play ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-010-0196-x
更新日期：2011-03-01 00:00:00
abstract：:The proneness of diseases and susceptibility towards drugs vary from person to person. At present, there is a strong demand for the personalization of drugs. The genetic signature behind proneness of the disease has been studied through a comprehensive 'octopodial approach'. All the genetic variants included in the ap...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-017-0559-7
更新日期：2017-07-01 00:00:00
abstract：:In theory, gene therapy is a simple concept which holds great promise as a cure for disease. In practice, however, considerable obstacles have to be overcome, including problems with safe and efficient gene delivery and stable gene expression. This review gives an overview on the history of gene therapy and analyses s...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s101420000011
更新日期：2000-09-01 00:00:00
abstract：:Intronless genes, a characteristic feature of prokaryotes, constitute a significant portion of the eukaryotic genomes. Our analysis revealed the presence of 11,109 (19.9%) and 5,846 (21.7%) intronless genes in rice and Arabidopsis genomes, respectively, belonging to different cellular role and gene ontology categories...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-007-0052-9
更新日期：2008-02-01 00:00:00
abstract：:MicroRNAs (miRNAs) are small RNAs (sRNAs) that regulate gene expression in development and adaptive responses to the environment. The early days in the sRNA field was one of the most exciting and promising moments in modern biology, attracting large investments to the understanding of the underlining mechanisms and th...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-016-0513-0
更新日期：2017-05-01 00:00:00
abstract：:In silico analysis together with cloning, molecular characterization and heterologous expression reports that the hypothetical protein All5371 of Anabaena sp. PCC7120 is a novel hydroperoxide scavenging protein similar to AhpD of bacteria. The presence of E(X)11CX HC(X)3H motif in All5371 confers peroxidase activity a...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0407-y
更新日期：2015-01-01 00:00:00
abstract：:The Maize Gene Discovery Project (MGDP) is a 5-year NSF-funded plant genome initiative that began in 1998. The MGDP collaboration involves researchers at six universities from diverse disciplines with the common goal of discovering new maize genes and developing tools for the phenotypic characterization of maize mutan...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-002-0078-y
更新日期：2003-03-01 00:00:00
abstract：:Gene order is largely collinear in the small-grained cereals, a feature which has proved helpful in both marker development and positional cloning. The accuracy of a virtual gene order map ("genome zipper") for barley (Hordeum vulgare), developed by combining a genetic map of this species with a large number of gene l...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-013-0327-2
更新日期：2013-08-01 00:00:00
abstract：:The ever-rising population of the twenty-first century together with the prevailing challenges, such as deteriorating quality of arable land and water, has placed a big challenge for plant breeders to satisfy human needs for food under erratic weather patterns. Rice, wheat, and maize are the major staple crops consume...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-019-00684-1
更新日期：2019-09-01 00:00:00
abstract：:The IbeA (ibe10) gene is an invasion determinant contributing to E. coli K1 invasion of the blood-brain barrier. This gene has been cloned and characterized from the chromosome of an invasive cerebrospinal fluid isolate of E. coli K1, strain RS218 (018:K1: H7). In the present study, a genetic island of meningitic E. c...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s101420100039
更新日期：2001-09-01 00:00:00
abstract：:Fungal species are potential dye decomposers since these secrete spectra of extracellular enzymes involved in catabolism. However, cellular mechanisms underlying azo dye catalysis and detoxification are incompletely understood and obscure. A potential strain designated as Penicillium oxalicum SAR-3 demonstrated broad-...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0405-0
更新日期：2014-12-01 00:00:00
abstract：:Nuclear genomes of human, animals, and plants are organized into subunits called chromosomes. When isolated into aqueous suspension, mitotic chromosomes can be classified using flow cytometry according to light scatter and fluorescence parameters. Chromosomes of interest can be purified by flow sorting if they can be ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-012-0293-0
更新日期：2012-08-01 00:00:00
