Characterization of EST-derived microsatellites in the wheat genome and development of eSSR markers.

Abstract:

:EST-derived microsatellites or simple sequence repeats (eSSR) occur in expressed sequence tags (EST). Here we report characteristics of eSSRs in the wheat genome, construction of consensus chromosome bin maps of SSR-containing ESTs ((SSR)ESTs), and development of eSSR markers for the 21 wheat chromosomes. A Perl script known as MISA was used to identify eSSRs in wheat ESTs available in the database http://wheat.pw.usda.gov/cgi-bin/ace/search/wEST ). Among 492,832 ESTs from the database, 36,520 (7.41%) contained 43,598 eSSRs. This is equivalent to 1 eSSR per 5.46 kb EST sequence. About 60% of the eSSRs were trinucleotides, 19.7% were mononucleotide, 16.7% were dinucleotides, and the remaining approximately 3% consisted of tetra-, penta-, and hexanucleotides. Among the identified eSSRs, (CCG/CGG)n is the most frequent (20.5%) followed by (A/T)n at 13.6%, (AAC/GTT)n at 11.7%, and (AG/CT)n at 8.7%. Among ESTs previously mapped to wheat chromosome bins, a total of 1,010 eSSR loci were derived from 341 (SSR)ESTs. Consensus chromosome bin maps showing the chromosome locations of (SSR)ESTs, SSR sequence motif, and cDNA library were constructed. A chi(2) test indicated that the distribution pattern of eSSR loci was generally similar to that of the original mapped ESTs in the wheat genome. Forty-eight (SSR)ESTs were converted into PCR-based eSSR markers, and 266 eSSR loci were mapped to specific chromosome arms using wheat cytogenetic stocks. The average polymorphism information content (0.45+/-0.16) of eSSR markers was lower than that reported for genomic SSRs (0.54+/-0.19), but higher than RFLPs (0.30+/-0.27). The eSSR markers were transferable among related Triticeae species, Triticum aestivum, T. durum, T. dicoccoides, Hordeum spontaneum, H. vulgare, and Secale cereale. The results confirm the presence of SSRs in expressed genes of wheat and demonstrate another application of ESTs in genomics research. eSSRs will be useful for gene tagging, gene cloning, and comparative genomics studies of cereal crops.

journal_name

Funct Integr Genomics

authors

Peng JH,Lapitan NL

doi

10.1007/s10142-004-0128-8

keywords:

subject

Has Abstract

pub_date

2005-04-01 00:00:00

pages

80-96

issue

2

eissn

1438-793X

issn

1438-7948

journal_volume

5

pub_type

杂志文章
  • The bioinformatics challenges in comparative analysis of cereal genomes-an overview.

    abstract::Comparative genomic analysis is the cornerstone of in silico-based approaches to understanding biological systems and processes across cereal species, such as rice, wheat and barley, in order to identify genes of agronomic interest. The size of the genomic repositories is nearly doubling every year, and this has signi...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章,评审

    doi:10.1007/s10142-004-0102-5

    authors: Bellgard M,Ye J,Gojobori T,Appels R

    更新日期:2004-03-01 00:00:00

  • Transcriptomic profiling of developing fiber in levant cotton (Gossypium herbaceum L.).

    abstract::Cotton (Gossypium spp.) is an imperative economic crop of the globe due to its natural textile fiber. Molecular mechanisms of fiber development have been greatly revealed in allotetraploid cotton but remained unexplored in Gossypium herbaceum. G. herbaceum can withstand the rigors of nature like drought and pests but ...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-017-0586-4

    authors: Parekh MJ,Kumar S,Fougat RS,Zala HN,Pandit RJ

    更新日期:2018-03-01 00:00:00

  • Deep-sequencing transcriptome analysis of field-grown Medicago sativa L. crown buds acclimated to freezing stress.

    abstract::Medicago sativa L. (alfalfa) 'Zhaodong' is an important forage legume that can safely survive in northern China where winter temperatures reach as low as -30 °C. Survival of alfalfa following freezing stress depends on the amount and revival ability of crown buds. In order to investigate the molecular mechanisms of fr...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-016-0500-5

    authors: Song L,Jiang L,Chen Y,Shu Y,Bai Y,Guo C

    更新日期:2016-09-01 00:00:00

  • Induction of DREB2A pathway with repression of E2F, jasmonic acid biosynthetic and photosynthesis pathways in cold acclimation-specific freeze-resistant wheat crown.

    abstract::Winter wheat lines can achieve cold acclimation (development of tolerance to freezing temperatures) and vernalization (delay in transition from vegetative to reproductive phase) in response to low non-freezing temperatures. To describe cold-acclimation-specific processes and pathways, we utilized cold acclimation tran...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-012-0303-2

    authors: Karki A,Horvath DP,Sutton F

    更新日期:2013-03-01 00:00:00

  • GA3 application in grapes (Vitis vinifera L.) modulates different sets of genes at cluster emergence, full bloom, and berry stage as revealed by RNA sequence-based transcriptome analysis.

    abstract::In grapes (Vitis vinifera L.), exogenous gibberellic acid (GA3) is applied at different stages of bunch development to achieve desirable bunch shape and berry size in seedless grapes used for table purpose. RNA sequence-based transcriptome analysis was used to understand the mechanism of GA3 action at cluster emergenc...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-018-0605-0

    authors: Upadhyay A,Maske S,Jogaiah S,Kadoo NY,Gupta VS

    更新日期:2018-07-01 00:00:00

  • A novel genetic island of meningitic Escherichia coli K1 containing the ibeA invasion gene (GimA): functional annotation and carbon-source-regulated invasion of human brain microvascular endothelial cells.

    abstract::The IbeA (ibe10) gene is an invasion determinant contributing to E. coli K1 invasion of the blood-brain barrier. This gene has been cloned and characterized from the chromosome of an invasive cerebrospinal fluid isolate of E. coli K1, strain RS218 (018:K1: H7). In the present study, a genetic island of meningitic E. c...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s101420100039

    authors: Huang SH,Chen YH,Kong G,Chen SH,Besemer J,Borodovsky M,Jong A

    更新日期:2001-09-01 00:00:00

  • A novel alkyl hydroperoxidase (AhpD) of Anabaena PCC7120 confers abiotic stress tolerance in Escherichia coli.

    abstract::In silico analysis together with cloning, molecular characterization and heterologous expression reports that the hypothetical protein All5371 of Anabaena sp. PCC7120 is a novel hydroperoxide scavenging protein similar to AhpD of bacteria. The presence of E(X)11CX HC(X)3H motif in All5371 confers peroxidase activity a...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-014-0407-y

    authors: Shrivastava AK,Singh S,Singh PK,Pandey S,Rai LC

    更新日期:2015-01-01 00:00:00

  • Advances in biotechnology and informatics to link variation in the genome to phenotypes in plants and animals.

    abstract::Advances in our understanding of genome structure provide consistent evidence for the existence of a core genome representing species classically defined by phenotype, as well as conditionally dispensable components of the genome that shows extensive variation between individuals of a given species. Generally, conserv...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章,评审

    doi:10.1007/s10142-013-0319-2

    authors: Appels R,Barrero R,Bellgard M

    更新日期:2013-03-01 00:00:00

  • Genomic sequencing reveals gene content, genomic organization, and recombination relationships in barley.

    abstract::Barley (Hordeum vulgare L.) is one of the most important large-genome cereals with extensive genetic resources available in the public sector. Studies of genome organization in barley have been limited primarily to genetic markers and sparse sequence data. Here we report sequence analysis of 417.5 kb DNA from four BAC...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-002-0055-5

    authors: Rostoks N,Park YJ,Ramakrishna W,Ma J,Druka A,Shiloff BA,SanMiguel PJ,Jiang Z,Brueggeman R,Sandhu D,Gill K,Bennetzen JL,Kleinhofs A

    更新日期:2002-05-01 00:00:00

  • Effect of herbicidal application of 2,4-dichlorophenoxyacetic acid in Arabidopsis.

    abstract::The whole genome expression pattern of Arabidopsis in response to the auxinic herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) was evaluated using the Affymetrix ATH1-121501 array. Arabidopsis plants were grown in vitro and were exposed to 1 mM 2,4-D for 1 h, after which time gene transcription levels were measured. I...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-004-0119-9

    authors: Raghavan C,Ong EK,Dalling MJ,Stevenson TW

    更新日期:2005-01-01 00:00:00

  • Whole transcriptome responses among females of the filariasis and arbovirus vector mosquito Culex pipiens implicate TGF-β signaling and chromatin modification as key drivers of diapause induction.

    abstract::Culex pipiens mosquitoes are important disease vectors inhabiting temperate zones, worldwide. The seasonal reduction in temperature and photoperiod accompanying late summer and early fall prompts female mosquitoes to enter diapause, a stage of developmental arrest and physiological conditioning that enhances survival ...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-015-0432-5

    authors: Hickner PV,Mori A,Zeng E,Tan JC,Severson DW

    更新日期:2015-07-01 00:00:00

  • Functional features of a single chromosome arm in wheat (1AL) determined from its structure.

    abstract::Bread wheat (Triticum aestivum L.) is one of the most important crops globally and a high priority for genetic improvement, but its large and complex genome has been seen as intractable to whole genome sequencing. Isolation of individual wheat chromosome arms has facilitated large-scale sequence analyses. However, so ...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-011-0250-3

    authors: Lucas SJ,Šimková H,Šafář J,Jurman I,Cattonaro F,Vautrin S,Bellec A,Berges H,Doležel J,Budak H

    更新日期:2012-03-01 00:00:00

  • The gene structure and promoter region of the vaccine target aminopeptidase H11 from the blood-sucking nematode parasite of ruminants, Haemonchus contortus.

    abstract::Aminopeptidase H11, an integral membrane glycoprotein present only in the gut of Haemonchus contortus, could provide substantial protection as shown by 90% reduction in fecal egg counts, while its recombinant version expressed in E. coli induced little. To investigate the characteristics further, we amplified mRNA of ...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-010-0172-5

    authors: Zhou QJ,Zhang HL,Jiang XL,Du AF

    更新日期:2010-11-01 00:00:00

  • Genome-wide analysis of intronless genes in rice and Arabidopsis.

    abstract::Intronless genes, a characteristic feature of prokaryotes, constitute a significant portion of the eukaryotic genomes. Our analysis revealed the presence of 11,109 (19.9%) and 5,846 (21.7%) intronless genes in rice and Arabidopsis genomes, respectively, belonging to different cellular role and gene ontology categories...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-007-0052-9

    authors: Jain M,Khurana P,Tyagi AK,Khurana JP

    更新日期:2008-02-01 00:00:00

  • Serpins in plants and green algae.

    abstract::Control of proteolysis is important for plant growth, development, responses to stress, and defence against insects and pathogens. Members of the serpin protein family are likely to play a critical role in this control through irreversible inhibition of endogenous and exogenous target proteinases. Serpins have been fo...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章,评审

    doi:10.1007/s10142-007-0059-2

    authors: Roberts TH,Hejgaard J

    更新日期:2008-02-01 00:00:00

  • Transcriptome analysis of the circadian clock gene BMAL1 deletion with opposite carcinogenic effects.

    abstract::We have previously reported that the deletion of BMAL1 gene has opposite effects in respect to its contribution to the pathways that are effective in the multistage carcinogenesis process. BMAL1 deletion sensitized nearly normal breast epithelial (MCF10A) and invasive breast cancer cells (MDA-MB-231) to cisplatin- and...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-020-00757-6

    authors: Emisoglu-Kulahli H,Gul S,Morgil H,Ozcan O,Aygenli F,Selvi S,Kavakli IH,Ozturk N

    更新日期:2021-01-01 00:00:00

  • Expression dynamics of metabolic and regulatory components across stages of panicle and seed development in indica rice.

    abstract::Carefully analyzed expression profiles can serve as a valuable reference for deciphering gene functions. We exploited the potential of whole genome microarrays to measure the spatial and temporal expression profiles of rice genes in 19 stages of vegetative and reproductive development. We could verify expression of 22...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-012-0274-3

    authors: Sharma R,Agarwal P,Ray S,Deveshwar P,Sharma P,Sharma N,Nijhawan A,Jain M,Singh AK,Singh VP,Khurana JP,Tyagi AK,Kapoor S

    更新日期:2012-06-01 00:00:00

  • Comparing three methods for variance estimation with duplicated high density oligonucleotide arrays.

    abstract::Microarray experiments are being increasingly used in molecular biology. A common task is to detect genes with differential expression across two experimental conditions, such as two different tissues or the same tissue at two time points of biological development. To take proper account of statistical variability, so...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-002-0066-2

    authors: Huang X,Pan W

    更新日期:2002-08-01 00:00:00

  • Progress in maize gene discovery: a project update.

    abstract::The Maize Gene Discovery Project (MGDP) is a 5-year NSF-funded plant genome initiative that began in 1998. The MGDP collaboration involves researchers at six universities from diverse disciplines with the common goal of discovering new maize genes and developing tools for the phenotypic characterization of maize mutan...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章,评审

    doi:10.1007/s10142-002-0078-y

    authors: Lunde CF,Morrow DJ,Roy LM,Walbot V

    更新日期:2003-03-01 00:00:00

  • Major intrinsic proteins (MIPs) in plants: a complex gene family with major impacts on plant phenotype.

    abstract::The ubiquitous cell membrane proteins called aquaporins are now firmly established as channel proteins that control the specific transport of water molecules across cell membranes in all living organisms. The aquaporins are thus likely to be of fundamental significance to all facets of plant growth and development aff...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章,评审

    doi:10.1007/s10142-007-0049-4

    authors: Forrest KL,Bhave M

    更新日期:2007-10-01 00:00:00

  • Gene networks in the synthesis and deposition of protein polymers during grain development of wheat.

    abstract::As the amino acid storing organelle, the protein bodies provide nutrients for embryo development, seed germination and early seedling growth through storage proteolysis in cereal plants, such as wheat and rice. In protein bodies, the monomeric and polymeric prolamins, i.e. gliadins and glutenins, form gluten and play ...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章,评审

    doi:10.1007/s10142-010-0196-x

    authors: She M,Ye X,Yan Y,Howit C,Belgard M,Ma W

    更新日期:2011-03-01 00:00:00

  • The entangled history of animal and plant microRNAs.

    abstract::MicroRNAs (miRNAs) are small RNAs (sRNAs) that regulate gene expression in development and adaptive responses to the environment. The early days in the sRNA field was one of the most exciting and promising moments in modern biology, attracting large investments to the understanding of the underlining mechanisms and th...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章,评审

    doi:10.1007/s10142-016-0513-0

    authors: Reis RS

    更新日期:2017-05-01 00:00:00

  • Cultivar-specific kinetics of gene induction during downy mildew early infection in grapevine.

    abstract::The oomycete pathogen Plasmopara viticola (Berk. et Curt.) Berl. et de Toni is the causing agent of the destructive downy mildew disease in grapevine. Despite the advances towards elucidation of grapevine resistance mechanisms to downy mildew, increased knowledge of the biological and genetic components of the pathosy...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-012-0261-8

    authors: Figueiredo A,Monteiro F,Fortes AM,Bonow-Rex M,Zyprian E,Sousa L,Pais MS

    更新日期:2012-06-01 00:00:00

  • Transcriptome analysis reveals diversified adaptation of Stipa purpurea along a drought gradient on the Tibetan Plateau.

    abstract::Natural selection drives species adaptations to biotic and abiotic stresses. Species distributed along a moisture gradient, such as Stipa purpurea, a dominant grass in alpine arid and semi-arid meadows on the Tibetan Plateau, provide an opportunity to evaluate the effects of long-term adaptation to differing degrees o...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-014-0419-7

    authors: Yang Y,Li X,Kong X,Ma L,Hu X,Yang Y

    更新日期:2015-05-01 00:00:00

  • Gene therapy--when a simple concept meets a complex reality. Review on gene therapy.

    abstract::In theory, gene therapy is a simple concept which holds great promise as a cure for disease. In practice, however, considerable obstacles have to be overcome, including problems with safe and efficient gene delivery and stable gene expression. This review gives an overview on the history of gene therapy and analyses s...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章,评审

    doi:10.1007/s101420000011

    authors: Grosshans H

    更新日期:2000-09-01 00:00:00

  • Genome-wide analysis of water-stress-responsive microRNA expression profile in tobacco roots.

    abstract::MicroRNAs (miRNAs) play a pivotal role in post-transcriptional regulation of gene expression in plants. In this study, we investigate miRNAs in an agronomically important common tobacco in China, named Honghua Dajinyuan (a drought-tolerant cultivar). Here, we report a comprehensive analysis of miRNA expression profile...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-014-0365-4

    authors: Yin F,Gao J,Liu M,Qin C,Zhang W,Yang A,Xia M,Zhang Z,Shen Y,Lin H,Luo C,Pan G

    更新日期:2014-06-01 00:00:00

  • Expansion and evolutionary patterns of GDSL-type esterases/lipases in Rosaceae genomes.

    abstract::GDSL-type esterase/lipase (GELP) is mainly characterized by a conserved GDSL domain at N terminus, and is widely found in all living species, both prokaryotes and eukaryotes. GELP gene family consists of a wide range of members playing important roles in plant physiological processes, such as development, stress respo...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-018-0620-1

    authors: Cao Y,Han Y,Meng D,Abdullah M,Yu J,Li D,Jin Q,Lin Y,Cai Y

    更新日期:2018-11-01 00:00:00

  • Chromosomes in the flow to simplify genome analysis.

    abstract::Nuclear genomes of human, animals, and plants are organized into subunits called chromosomes. When isolated into aqueous suspension, mitotic chromosomes can be classified using flow cytometry according to light scatter and fluorescence parameters. Chromosomes of interest can be purified by flow sorting if they can be ...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章,评审

    doi:10.1007/s10142-012-0293-0

    authors: Doležel J,Vrána J,Safář J,Bartoš J,Kubaláková M,Simková H

    更新日期:2012-08-01 00:00:00

  • Identification of candidate genes JcARF19 and JcIAA9 associated with seed size traits in Jatropha.

    abstract::Jatropha curcas is a new promising bioenergy crop due to the high oil content in its seeds that can be converted into biodiesel. Seed size, a major determinant of Jatropha oil yield, is a target trait for Jatropha breeding. Due to the vital roles of phytohormone auxin in controlling seed and fruit development, we scre...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-014-0400-5

    authors: Ye J,Liu P,Zhu C,Qu J,Wang X,Sun Y,Sun F,Jiang Y,Yue G,Wang C

    更新日期:2014-12-01 00:00:00

  • Identification and functional characterization of four novel aldo/keto reductases in Anabaena sp. PCC 7120 by integrating wet lab with in silico approaches.

    abstract::Aldo/keto reductases (AKRs) constitute a multitasking protein family that catalyzes diverse metabolic transformations including detoxification of stress generated reactive aldehydes. Yet this important protein family is poorly understood particularly in cyanobacteria, the ecologically most diverse and significant grou...

    journal_title:Functional & integrative genomics

    pub_type: 杂志文章

    doi:10.1007/s10142-017-0547-y

    authors: Agrawal C,Yadav S,Rai S,Chatterjee A,Sen S,Rai R,Rai LC

    更新日期:2017-07-01 00:00:00