Abstract：
:Fungal species are potential dye decomposers since these secrete spectra of extracellular enzymes involved in catabolism. However, cellular mechanisms underlying azo dye catalysis and detoxification are incompletely understood and obscure. A potential strain designated as Penicillium oxalicum SAR-3 demonstrated broad-spectrum catabolic ability of different azo dyes. A forward suppression subtractive hybridization (SSH) cDNA library of P. oxalicum SAR-3 constructed in presence and absence of azo dye Acid Red 183 resulted in identification of 183 unique expressed sequence tags (ESTs) which were functionally classified into 12 functional categories. A number of novel genes that affect specifically organic azo dye degradation were discovered. Although the ABC transporters and peroxidases emerged as prominent hot spot for azo dye detoxification, we also identified a number of proteins that are more proximally related to stress-responsive gene expression. Majority of the ESTs (29.5%) were grouped as hypothetical/unknown indicating the presence of putatively novel genes. Analysis of few ESTs through quantitative real-time reverse transcription polymerase chain reaction revealed their possible role in AR183 degradation. The ESTs identified in the SSH library provide a novel insight on the transcripts that are expressed in P. oxalicum strain SAR-3 in response to AR183.
journal_name
Funct Integr Genomicsjournal_title
Functional & integrative genomicsauthors
Saroj S,Kumar K,Prasad M,Singh RPdoi
10.1007/s10142-014-0405-0subject
Has Abstractpub_date
2014-12-01 00:00:00pages
631-42issue
4eissn
1438-793Xissn
1438-7948journal_volume
14pub_type
杂志文章abstract：:MicroRNAs (miRNAs) are small RNAs (sRNAs) that regulate gene expression in development and adaptive responses to the environment. The early days in the sRNA field was one of the most exciting and promising moments in modern biology, attracting large investments to the understanding of the underlining mechanisms and th...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-016-0513-0
更新日期：2017-05-01 00:00:00
abstract：:The assembly of a 1.3-Mb size region of the wheat genome has provided the opportunity to study a recent nuclear mitochondrial DNA insertion (NUMT). In the present study, we have studied two bacterial artificial chromosomes (BACs) and characterized a 52-kb NUMT segment from the tetraploid and hexaploid wheat BAC librar...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-011-0237-0
更新日期：2011-12-01 00:00:00
abstract：:GDSL-type esterase/lipase (GELP) is mainly characterized by a conserved GDSL domain at N terminus, and is widely found in all living species, both prokaryotes and eukaryotes. GELP gene family consists of a wide range of members playing important roles in plant physiological processes, such as development, stress respo...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-018-0620-1
更新日期：2018-11-01 00:00:00
abstract：:MicroRNAs (miRNAs) play a pivotal role in post-transcriptional regulation of gene expression in plants. In this study, we investigate miRNAs in an agronomically important common tobacco in China, named Honghua Dajinyuan (a drought-tolerant cultivar). Here, we report a comprehensive analysis of miRNA expression profile...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0365-4
更新日期：2014-06-01 00:00:00
abstract：:Water temperature affects the survival, growth, immunity, reproduction, and productivity of farmed fish. The temperature beyond suitable range will disrupt the normal physiological activity. Common carp (Cyprinus carpio L.) is a representative eurythermic fish; they are able to sense and respond to changes in water te...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-018-0643-7
更新日期：2019-03-01 00:00:00
abstract：:The whole genome expression pattern of Arabidopsis in response to the auxinic herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) was evaluated using the Affymetrix ATH1-121501 array. Arabidopsis plants were grown in vitro and were exposed to 1 mM 2,4-D for 1 h, after which time gene transcription levels were measured. I...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-004-0119-9
更新日期：2005-01-01 00:00:00
abstract：:Microarray experiments are being increasingly used in molecular biology. A common task is to detect genes with differential expression across two experimental conditions, such as two different tissues or the same tissue at two time points of biological development. To take proper account of statistical variability, so...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-002-0066-2
更新日期：2002-08-01 00:00:00
abstract：:Campylobacter jejuni remains a major cause of human gastroenteritis with estimated annual incidence rate of 450 million infections worldwide. C. jejuni is a major burden to public health in both socioeconomically developing and industrialized nations. Virulence determinants involved in C. jejuni pathogenesis are multi...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-016-0530-z
更新日期：2017-01-01 00:00:00
abstract：:Aminopeptidase H11, an integral membrane glycoprotein present only in the gut of Haemonchus contortus, could provide substantial protection as shown by 90% reduction in fecal egg counts, while its recombinant version expressed in E. coli induced little. To investigate the characteristics further, we amplified mRNA of ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-010-0172-5
更新日期：2010-11-01 00:00:00
abstract：:Abiotic stress tolerance is a complex trait regulated by multiple genes and gene networks in plants. A range of abiotic stresses are known to limit rice productivity. Meta-transcriptomics has emerged as a powerful approach to decipher stress-associated molecular network in model crops. However, retaining specificity o...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,meta分析
doi：10.1007/s10142-019-00697-w
更新日期：2020-01-01 00:00:00
abstract：:Drought is a major abiotic stress affecting crop productivity and quality. As a class of noncoding RNA, microRNA (miRNA) plays important roles in plant growth, development, and stress response. However, their response and roles in tomato drought stress is largely unknown. Here, by using high-throughput sequencing, we ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-017-0575-7
更新日期：2018-01-01 00:00:00
abstract：:The ubiquitous cell membrane proteins called aquaporins are now firmly established as channel proteins that control the specific transport of water molecules across cell membranes in all living organisms. The aquaporins are thus likely to be of fundamental significance to all facets of plant growth and development aff...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-007-0049-4
更新日期：2007-10-01 00:00:00
abstract：:Auxin response factors (ARFs) are recently discovered transcription factors that bind with auxin response elements (AuxRE, TGTCTC) to regulate the expression of early auxin-responsive genes. To our knowledge, the ARF gene family has never been characterized in cotton, the most important fiber crop in the world. In thi...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-015-0437-0
更新日期：2015-07-01 00:00:00
abstract：:Although the economic value of wheat flour is determined by the complement of gluten proteins, these proteins have been challenging to study because of the complexity of the major protein groups and the tremendous sequence diversity among wheat cultivars. The completion of a high-quality wheat genome sequence from the...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-019-00694-z
更新日期：2020-01-01 00:00:00
abstract：:Natural selection drives species adaptations to biotic and abiotic stresses. Species distributed along a moisture gradient, such as Stipa purpurea, a dominant grass in alpine arid and semi-arid meadows on the Tibetan Plateau, provide an opportunity to evaluate the effects of long-term adaptation to differing degrees o...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0419-7
更新日期：2015-05-01 00:00:00
abstract：:Seasonal estrus is a key factor limiting animal fertility, and understanding the molecular mechanisms that regulate animal estrus is important for improving animal fertility. The pituitary gland, which is the most important endocrine gland in mammals, plays an important role in regulating the physiological processes s...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-020-00735-y
更新日期：2020-07-01 00:00:00
abstract：:Nuclear genomes of human, animals, and plants are organized into subunits called chromosomes. When isolated into aqueous suspension, mitotic chromosomes can be classified using flow cytometry according to light scatter and fluorescence parameters. Chromosomes of interest can be purified by flow sorting if they can be ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-012-0293-0
更新日期：2012-08-01 00:00:00
abstract：:Winter wheat lines can achieve cold acclimation (development of tolerance to freezing temperatures) and vernalization (delay in transition from vegetative to reproductive phase) in response to low non-freezing temperatures. To describe cold-acclimation-specific processes and pathways, we utilized cold acclimation tran...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-012-0303-2
更新日期：2013-03-01 00:00:00
abstract：:The expression of many genes encoding transcriptional activators in prokaryotes and eukaryotes is upregulated through positive feedback activation. During positive feedback activation, a transcriptional activator binds to its own promoter and thus increases its own expression as well as the expression of its target ge...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s101420000001
更新日期：2000-05-01 00:00:00
abstract：:Rhizoctonia solani AG1-IA is a soil-borne necrotrophic pathogen that causes devastating rice sheath blight disease in rice-growing regions worldwide. Sclerotia play an important role in the life cycle of R. solani AG1-IA. In this study, RNA sequencing was used to investigate the transcriptomic dynamics of sclerotial d...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-019-00677-0
更新日期：2019-09-01 00:00:00
abstract：:Carefully analyzed expression profiles can serve as a valuable reference for deciphering gene functions. We exploited the potential of whole genome microarrays to measure the spatial and temporal expression profiles of rice genes in 19 stages of vegetative and reproductive development. We could verify expression of 22...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-012-0274-3
更新日期：2012-06-01 00:00:00
abstract：:A cDNA library containing approximately 5,000 clones from germinating barley embryos was constructed and used to examine the variation in gene expression patterns during the first 4 days postimbibition. The expression profiles of embryos (including scutellum) from 4 to 96 h postimbibition were compared to a reference ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-005-0133-6
更新日期：2005-07-01 00:00:00
abstract：:Culex pipiens mosquitoes are important disease vectors inhabiting temperate zones, worldwide. The seasonal reduction in temperature and photoperiod accompanying late summer and early fall prompts female mosquitoes to enter diapause, a stage of developmental arrest and physiological conditioning that enhances survival ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-015-0432-5
更新日期：2015-07-01 00:00:00
abstract：:Control of proteolysis is important for plant growth, development, responses to stress, and defence against insects and pathogens. Members of the serpin protein family are likely to play a critical role in this control through irreversible inhibition of endogenous and exogenous target proteinases. Serpins have been fo...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-007-0059-2
更新日期：2008-02-01 00:00:00
abstract：:Cotton (Gossypium spp.) is an imperative economic crop of the globe due to its natural textile fiber. Molecular mechanisms of fiber development have been greatly revealed in allotetraploid cotton but remained unexplored in Gossypium herbaceum. G. herbaceum can withstand the rigors of nature like drought and pests but ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-017-0586-4
更新日期：2018-03-01 00:00:00
abstract：:The Maize Gene Discovery Project (MGDP) is a 5-year NSF-funded plant genome initiative that began in 1998. The MGDP collaboration involves researchers at six universities from diverse disciplines with the common goal of discovering new maize genes and developing tools for the phenotypic characterization of maize mutan...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-002-0078-y
更新日期：2003-03-01 00:00:00
abstract：:The IbeA (ibe10) gene is an invasion determinant contributing to E. coli K1 invasion of the blood-brain barrier. This gene has been cloned and characterized from the chromosome of an invasive cerebrospinal fluid isolate of E. coli K1, strain RS218 (018:K1: H7). In the present study, a genetic island of meningitic E. c...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s101420100039
更新日期：2001-09-01 00:00:00
abstract：:The symbiotic N(2)-fixing alpha-proteobacterium Sinorhizobium meliloti has three replicons: a circular chromosome (3.7 Mb) and two smaller replicons, pSymA (1.4 Mb) and pSymB (1.7 Mb). Sequence analysis has revealed that an essential gene is carried on pSymB, which brings into question whether pSymB should be consider...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-002-0068-0
更新日期：2002-11-01 00:00:00
abstract：:Gastrointestinal nematodes of the genus Cooperia are arguably the most important parasites of cattle. The bovine jejunal transcriptome was characterized in response to Cooperia oncophora infection using RNA-seq technology. Approximately 71% of the 25,670 bovine genes were detected in the jejunal transcriptome. However...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-011-0259-7
更新日期：2012-03-01 00:00:00
abstract：:The proneness of diseases and susceptibility towards drugs vary from person to person. At present, there is a strong demand for the personalization of drugs. The genetic signature behind proneness of the disease has been studied through a comprehensive 'octopodial approach'. All the genetic variants included in the ap...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-017-0559-7
更新日期：2017-07-01 00:00:00
