Abstract：
:Drought is a major abiotic stress affecting crop productivity and quality. As a class of noncoding RNA, microRNA (miRNA) plays important roles in plant growth, development, and stress response. However, their response and roles in tomato drought stress is largely unknown. Here, by using high-throughput sequencing, we compared the miRNA profiles before and after drought treatment in two tomato genotypes: M82, a drought-sensitive cultivated tomato (Solanum lycopersicum), and IL2-5, a drought-tolerant introgression line derived from M82 and the tomato wild species S. pennellii (LA0716). A total of 108 conserved and 208 novel miRNAs were identified, among them, 32 and 68 were significantly changed in expression after stress. Further, 1936 putative target genes were predicted for those differentially-expressed miRNAs. Gene ontology and pathway analysis showed that many of the target genes were involved in stress resistance, such as genes in GO terms including response to stress, defense response, response to stimulus, phosphorylation, and signal transduction. Our results suggested that miRNAs play an essential role in the drought response of tomato. This work will help to further characterize specific miRNAs functioning in drought tolerance.
journal_name
Funct Integr Genomicsjournal_title
Functional & integrative genomicsauthors
Liu M,Yu H,Zhao G,Huang Q,Lu Y,Ouyang Bdoi
10.1007/s10142-017-0575-7subject
Has Abstractpub_date
2018-01-01 00:00:00pages
67-78issue
1eissn
1438-793Xissn
1438-7948pii
10.1007/s10142-017-0575-7journal_volume
18pub_type
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