Abstract：
:The ATP-binding cassette (ABC) transporters are a large family of proteins responsible for the translocation of a variety of compounds across the membranes of both prokaryotes and eukaryotes. The inter-protein and intra-protein interactions in these traffic ATPases are still only poorly understood. In the present study we describe, for the first time, an extensive yeast two-hybrid (Y2H)-based analysis of the interactions of the cytoplasmic loops of the yeast pleiotropic drug resistance (Pdr) protein, Pdr5p, an ABC transporter of Saccharomyces cerevisiae. Four of the major cytosolic loops that have been predicted for this protein [including the two nucleotide-binding domain (NBD)-containing loops and the cytosolic C-terminal region] were subjected to an extensive inter-domain interaction study in addition to being used as baits to identify potential interacting proteins within the cell using the Y2H system. Results of these studies have revealed that the first cytosolic loop (CL1)--containing the first NBD domain--and also the C-terminal region of Pdr5p, interact with several candidate proteins. The possibility of an interaction between the CL1 loops of two neighboring Pdr5p molecules was also indicated, which could possibly have implications for dimerization of this protein.
journal_name
Funct Integr Genomicsjournal_title
Functional & integrative genomicsauthors
Subba Rao G,Bachhawat AK,Gupta CMdoi
10.1007/s10142-001-0040-4keywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
357-66issue
6eissn
1438-793Xissn
1438-7948journal_volume
1pub_type
杂志文章abstract：:In theory, gene therapy is a simple concept which holds great promise as a cure for disease. In practice, however, considerable obstacles have to be overcome, including problems with safe and efficient gene delivery and stable gene expression. This review gives an overview on the history of gene therapy and analyses s...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s101420000011
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journal_title：Functional & integrative genomics
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-016-0530-z
更新日期：2017-01-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-015-0432-5
更新日期：2015-07-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0379-y
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journal_title：Functional & integrative genomics
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-011-0250-3
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-011-0237-0
更新日期：2011-12-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-008-0075-x
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
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更新日期：2012-03-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0405-0
更新日期：2014-12-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-010-0172-5
更新日期：2010-11-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s101420000001
更新日期：2000-05-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-017-0547-y
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-015-0472-x
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-016-0479-y
更新日期：2016-07-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-017-0559-7
更新日期：2017-07-01 00:00:00
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journal_title：Functional & integrative genomics
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doi：10.1007/s10142-005-0133-6
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journal_title：Functional & integrative genomics
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journal_title：Functional & integrative genomics
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更新日期：2019-03-01 00:00:00
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journal_title：Functional & integrative genomics
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doi：10.1007/s10142-018-0605-0
更新日期：2018-07-01 00:00:00
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journal_title：Functional & integrative genomics
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更新日期：2020-07-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-012-0261-8
更新日期：2012-06-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章,meta分析
doi：10.1007/s10142-019-00697-w
更新日期：2020-01-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-013-0358-8
更新日期：2014-06-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-016-0500-5
更新日期：2016-09-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0365-4
更新日期：2014-06-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-012-0293-0
更新日期：2012-08-01 00:00:00
