Abstract：
:In silico analysis together with cloning, molecular characterization and heterologous expression reports that the hypothetical protein All5371 of Anabaena sp. PCC7120 is a novel hydroperoxide scavenging protein similar to AhpD of bacteria. The presence of E(X)11CX HC(X)3H motif in All5371 confers peroxidase activity and closeness to bacterial AhpD which is also reflected by its highest 3D structure homology with Rhodospirillum rubrum AhpD. Heterologous expression of all5371 complimented for ahpC and conferred resistance in MJF178 strain (ahpCF::Km) of Escherichia coli. All5371 reduced the organic peroxide more efficiently than inorganic peroxide and the recombinant E. coli strain following exposure to H2O2, CdCl2, CuCl2, heat, UV-B and carbofuron registered increased growth over wild-type and mutant E. coli transformed with empty vector. Appreciable expression of all5371 in Anabaena sp. PCC7120 as measured by qRT-PCR under selected stresses and their tolerance against H2O2, tBOOH, CuOOH and menadione attested its role in stress tolerance. In view of the above, All5371 of Anabaena PCC7120 emerged as a new hydroperoxide detoxifying protein.
journal_name
Funct Integr Genomicsjournal_title
Functional & integrative genomicsauthors
Shrivastava AK,Singh S,Singh PK,Pandey S,Rai LCdoi
10.1007/s10142-014-0407-ysubject
Has Abstractpub_date
2015-01-01 00:00:00pages
77-92issue
1eissn
1438-793Xissn
1438-7948journal_volume
15pub_type
杂志文章abstract：:GDSL-type esterase/lipase (GELP) is mainly characterized by a conserved GDSL domain at N terminus, and is widely found in all living species, both prokaryotes and eukaryotes. GELP gene family consists of a wide range of members playing important roles in plant physiological processes, such as development, stress respo...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-018-0620-1
更新日期：2018-11-01 00:00:00
abstract：:Fungal species are potential dye decomposers since these secrete spectra of extracellular enzymes involved in catabolism. However, cellular mechanisms underlying azo dye catalysis and detoxification are incompletely understood and obscure. A potential strain designated as Penicillium oxalicum SAR-3 demonstrated broad-...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0405-0
更新日期：2014-12-01 00:00:00
abstract：:The ever-rising population of the twenty-first century together with the prevailing challenges, such as deteriorating quality of arable land and water, has placed a big challenge for plant breeders to satisfy human needs for food under erratic weather patterns. Rice, wheat, and maize are the major staple crops consume...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-019-00684-1
更新日期：2019-09-01 00:00:00
abstract：:Aminopeptidase H11, an integral membrane glycoprotein present only in the gut of Haemonchus contortus, could provide substantial protection as shown by 90% reduction in fecal egg counts, while its recombinant version expressed in E. coli induced little. To investigate the characteristics further, we amplified mRNA of ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-010-0172-5
更新日期：2010-11-01 00:00:00
abstract：:Jatropha curcas is a new promising bioenergy crop due to the high oil content in its seeds that can be converted into biodiesel. Seed size, a major determinant of Jatropha oil yield, is a target trait for Jatropha breeding. Due to the vital roles of phytohormone auxin in controlling seed and fruit development, we scre...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0400-5
更新日期：2014-12-01 00:00:00
abstract：:Campylobacter jejuni remains a major cause of human gastroenteritis with estimated annual incidence rate of 450 million infections worldwide. C. jejuni is a major burden to public health in both socioeconomically developing and industrialized nations. Virulence determinants involved in C. jejuni pathogenesis are multi...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-016-0530-z
更新日期：2017-01-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0379-y
更新日期：2014-09-01 00:00:00
abstract：:Medicago sativa L. (alfalfa) 'Zhaodong' is an important forage legume that can safely survive in northern China where winter temperatures reach as low as -30 °C. Survival of alfalfa following freezing stress depends on the amount and revival ability of crown buds. In order to investigate the molecular mechanisms of fr...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-016-0500-5
更新日期：2016-09-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-004-0102-5
更新日期：2004-03-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-004-0119-9
更新日期：2005-01-01 00:00:00
abstract：:The Maize Gene Discovery Project (MGDP) is a 5-year NSF-funded plant genome initiative that began in 1998. The MGDP collaboration involves researchers at six universities from diverse disciplines with the common goal of discovering new maize genes and developing tools for the phenotypic characterization of maize mutan...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-002-0078-y
更新日期：2003-03-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-012-0274-3
更新日期：2012-06-01 00:00:00
abstract：:Advances in our understanding of genome structure provide consistent evidence for the existence of a core genome representing species classically defined by phenotype, as well as conditionally dispensable components of the genome that shows extensive variation between individuals of a given species. Generally, conserv...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-013-0319-2
更新日期：2013-03-01 00:00:00
abstract：:Gene order is largely collinear in the small-grained cereals, a feature which has proved helpful in both marker development and positional cloning. The accuracy of a virtual gene order map ("genome zipper") for barley (Hordeum vulgare), developed by combining a genetic map of this species with a large number of gene l...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-013-0327-2
更新日期：2013-08-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-002-0068-0
更新日期：2002-11-01 00:00:00
abstract：:The photosynthetic organs of the barley spike (lemma, palea, and awn) are considered resistant to drought. However, there is little information about gene expression in the spike organs under drought conditions. We compared response of the transcriptome of the lemma, palea, awn, and seed to drought stress using the Ba...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-009-0149-4
更新日期：2010-05-01 00:00:00
abstract：:Auxin response factors (ARFs) are recently discovered transcription factors that bind with auxin response elements (AuxRE, TGTCTC) to regulate the expression of early auxin-responsive genes. To our knowledge, the ARF gene family has never been characterized in cotton, the most important fiber crop in the world. In thi...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-015-0437-0
更新日期：2015-07-01 00:00:00
abstract：:A cDNA library containing approximately 5,000 clones from germinating barley embryos was constructed and used to examine the variation in gene expression patterns during the first 4 days postimbibition. The expression profiles of embryos (including scutellum) from 4 to 96 h postimbibition were compared to a reference ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-005-0133-6
更新日期：2005-07-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-010-0196-x
更新日期：2011-03-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-011-0250-3
更新日期：2012-03-01 00:00:00
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journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-011-0252-1
更新日期：2012-03-01 00:00:00
abstract：:EST-derived microsatellites or simple sequence repeats (eSSR) occur in expressed sequence tags (EST). Here we report characteristics of eSSRs in the wheat genome, construction of consensus chromosome bin maps of SSR-containing ESTs ((SSR)ESTs), and development of eSSR markers for the 21 wheat chromosomes. A Perl scrip...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-004-0128-8
更新日期：2005-04-01 00:00:00
abstract：:Aldo/keto reductases (AKRs) constitute a multitasking protein family that catalyzes diverse metabolic transformations including detoxification of stress generated reactive aldehydes. Yet this important protein family is poorly understood particularly in cyanobacteria, the ecologically most diverse and significant grou...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-017-0547-y
更新日期：2017-07-01 00:00:00
abstract：:Control of proteolysis is important for plant growth, development, responses to stress, and defence against insects and pathogens. Members of the serpin protein family are likely to play a critical role in this control through irreversible inhibition of endogenous and exogenous target proteinases. Serpins have been fo...
journal_title：Functional & integrative genomics
pub_type： 杂志文章,评审
doi：10.1007/s10142-007-0059-2
更新日期：2008-02-01 00:00:00
abstract：:Late blight is a serious economic threat to potato crop, sometimes leading to complete crop loss. The resistance in potato to late blight can be qualitative or quantitative in nature. Qualitative resistance is not durable. Though quantitative resistance is durable, the breeding is challenging due to polygenic inherita...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-013-0358-8
更新日期：2014-06-01 00:00:00
abstract：:Rhizoctonia solani AG1-IA is a soil-borne necrotrophic pathogen that causes devastating rice sheath blight disease in rice-growing regions worldwide. Sclerotia play an important role in the life cycle of R. solani AG1-IA. In this study, RNA sequencing was used to investigate the transcriptomic dynamics of sclerotial d...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-019-00677-0
更新日期：2019-09-01 00:00:00
abstract：:Cotton (Gossypium spp.) is an imperative economic crop of the globe due to its natural textile fiber. Molecular mechanisms of fiber development have been greatly revealed in allotetraploid cotton but remained unexplored in Gossypium herbaceum. G. herbaceum can withstand the rigors of nature like drought and pests but ...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-017-0586-4
更新日期：2018-03-01 00:00:00
abstract：:The oomycete pathogen Plasmopara viticola (Berk. et Curt.) Berl. et de Toni is the causing agent of the destructive downy mildew disease in grapevine. Despite the advances towards elucidation of grapevine resistance mechanisms to downy mildew, increased knowledge of the biological and genetic components of the pathosy...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-012-0261-8
更新日期：2012-06-01 00:00:00
abstract：:The assembly of a 1.3-Mb size region of the wheat genome has provided the opportunity to study a recent nuclear mitochondrial DNA insertion (NUMT). In the present study, we have studied two bacterial artificial chromosomes (BACs) and characterized a 52-kb NUMT segment from the tetraploid and hexaploid wheat BAC librar...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-011-0237-0
更新日期：2011-12-01 00:00:00
abstract：:MicroRNAs (miRNAs) play a pivotal role in post-transcriptional regulation of gene expression in plants. In this study, we investigate miRNAs in an agronomically important common tobacco in China, named Honghua Dajinyuan (a drought-tolerant cultivar). Here, we report a comprehensive analysis of miRNA expression profile...
journal_title：Functional & integrative genomics
pub_type： 杂志文章
doi：10.1007/s10142-014-0365-4
更新日期：2014-06-01 00:00:00
