Abstract:
:MicroRNAs (miRNAs) play a pivotal role in post-transcriptional regulation of gene expression in plants. In this study, we investigate miRNAs in an agronomically important common tobacco in China, named Honghua Dajinyuan (a drought-tolerant cultivar). Here, we report a comprehensive analysis of miRNA expression profiles in mock-treat grown (CK) and 20 % polyethylene glycol-grown (PEG-grown) tobacco roots using a high-throughput sequencing approach. A total of 656 unique miRNAs representing 53 miRNA families were identified in the two libraries, of which 286 unique miRNAs representing 162 microRNAs were differentially expressed. In addition, nine differentially expressed microRNAs selected from different expressed miRNA family with high abundance were subjected to further analysis and validated by quantitative real-time PCR (Q-PCR). In addition, the expression pattern of these identified candidate conserved miRNA and target genes of three identified miRNA (nta-miR172b, nta-miR156i, and nta-miR160a) were also validated by Q-PCR. Gene ontology (GO) enrichment analysis suggests that the putative target genes of these differentially expressed miRNAs are involved in metabolic process and response to stimulus. In particular, 25 target genes are involved in regulating plant hormone signal transduction and metabolism, indicating that these association microRNAs may play important regulatory roles in responding to PEG resistance. Moreover, this study adds a significant number of novel miRNAs to the tobacco miRNome.
journal_name
Funct Integr Genomicsjournal_title
Functional & integrative genomicsauthors
Yin F,Gao J,Liu M,Qin C,Zhang W,Yang A,Xia M,Zhang Z,Shen Y,Lin H,Luo C,Pan Gdoi
10.1007/s10142-014-0365-4subject
Has Abstractpub_date
2014-06-01 00:00:00pages
319-32issue
2eissn
1438-793Xissn
1438-7948journal_volume
14pub_type
杂志文章abstract::In grapes (Vitis vinifera L.), exogenous gibberellic acid (GA3) is applied at different stages of bunch development to achieve desirable bunch shape and berry size in seedless grapes used for table purpose. RNA sequence-based transcriptome analysis was used to understand the mechanism of GA3 action at cluster emergenc...
journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-018-0605-0
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journal_title:Functional & integrative genomics
pub_type: 杂志文章,评审
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journal_title:Functional & integrative genomics
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journal_title:Functional & integrative genomics
pub_type: 杂志文章,评审
doi:10.1007/s10142-012-0293-0
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-009-0149-4
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-011-0252-1
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-012-0303-2
更新日期:2013-03-01 00:00:00
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journal_title:Functional & integrative genomics
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journal_title:Functional & integrative genomics
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journal_title:Functional & integrative genomics
pub_type: 杂志文章,评审
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s101420100039
更新日期:2001-09-01 00:00:00
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-009-0129-8
更新日期:2009-11-01 00:00:00
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journal_title:Functional & integrative genomics
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-017-0547-y
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-004-0128-8
更新日期:2005-04-01 00:00:00
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-014-0379-y
更新日期:2014-09-01 00:00:00
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journal_title:Functional & integrative genomics
pub_type: 杂志文章,评审
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journal_title:Functional & integrative genomics
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journal_title:Functional & integrative genomics
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doi:10.1007/s10142-008-0075-x
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journal_title:Functional & integrative genomics
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-018-0620-1
更新日期:2018-11-01 00:00:00
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-015-0432-5
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-002-0066-2
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journal_title:Functional & integrative genomics
pub_type: 杂志文章,评审
doi:10.1007/s10142-007-0049-4
更新日期:2007-10-01 00:00:00
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journal_title:Functional & integrative genomics
pub_type: 杂志文章,meta分析
doi:10.1007/s10142-019-00697-w
更新日期:2020-01-01 00:00:00
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journal_title:Functional & integrative genomics
pub_type: 杂志文章,评审
doi:10.1007/s10142-007-0059-2
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
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更新日期:2016-07-01 00:00:00
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-016-0530-z
更新日期:2017-01-01 00:00:00
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journal_title:Functional & integrative genomics
pub_type: 杂志文章
doi:10.1007/s10142-017-0586-4
更新日期:2018-03-01 00:00:00