Abstract：
:The expression of many genes encoding transcriptional activators in prokaryotes and eukaryotes is upregulated through positive feedback activation. During positive feedback activation, a transcriptional activator binds to its own promoter and thus increases its own expression as well as the expression of its target genes. In the simplest case, increased levels of the transcriptional activator can be directly correlated with increased expression of its target genes. In this study, we present a gene expression system, designated feedforward loop (FFL) system, which makes use of this kind of positive feedback regulation for the expression of plant transgenes. We show in transient and stable transformation experiments that such a system is functional and that it can be used to obtain high level gene expression in plants. We also provide evidence that the transgene is ubiquitously expressed when using the FFL system. Finally, we discuss the possibilities of using FFL gene expression systems for applications in plant molecular genetics and biotechnology.
journal_name
Funct Integr Genomicsjournal_title
Functional & integrative genomicsauthors
Schwechheimer C,Corke FM,Smith CH,Bevan Mdoi
10.1007/s101420000001keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
35-43issue
1eissn
1438-793Xissn
1438-7948journal_volume
1pub_type
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pub_type： 杂志文章,评审
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