Abstract:
:Aldo/keto reductases (AKRs) constitute a multitasking protein family that catalyzes diverse metabolic transformations including detoxification of stress generated reactive aldehydes. Yet this important protein family is poorly understood particularly in cyanobacteria, the ecologically most diverse and significant group of micro-organisms. Present study is an attempt to characterize all putative AKRs of Anabaena sp. PCC 7120. In silico analysis, it revealed the presence of at least four putative AKRs in Anabaena PCC7120 genome. All four proteins share less than 40% sequence identity with each other and also with the identified members of AKR superfamily and hence deserve to be assigned in new families. Dissimilarity in sequences is also reflected through their substrate specificity. While reduction of trans-2-nonenal, a LPO-derived reactive aldehyde was common across the four proteins, these proteins were found to be activated during heat, salt, Cd, As, and butachlor treatments, and their ectopic expression in Escherichia coli conferred tolerance to the above abiotic stresses. These findings affirm the role of AKRs in providing a broad tolerance to environmental stresses conceivably by detoxifying the stress-generated reactive aldehydes.
journal_name
Funct Integr Genomicsjournal_title
Functional & integrative genomicsauthors
Agrawal C,Yadav S,Rai S,Chatterjee A,Sen S,Rai R,Rai LCdoi
10.1007/s10142-017-0547-ysubject
Has Abstractpub_date
2017-07-01 00:00:00pages
413-425issue
4eissn
1438-793Xissn
1438-7948pii
10.1007/s10142-017-0547-yjournal_volume
17pub_type
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