Abstract:
:Reactive oxygen species (ROS) are critically important chemical intermediates in biological studies, due to their multiple physiologically essential functions and their often pathologically deleterious effects. Consequently, it is vital that their presence in biological samples has to be quantifiable. However, their high activity, very short life span and extremely low concentrations make ROS measurement a scientifically challenging subject for researchers. One of the widespread methods for ROS detection, based on the oxidation of the non-fluorescent probe 2',7'-dichlorodihydrofluorescein (DCFH(2)) to yield the highly fluorescent 2',7'-dichlorofluorescein (DCF), was developed more than 40 years ago. However, from its initial application, argumentative questions have arisen regarding its action mechanisms, reaction principles and especially its specificity. Herein, the authors attempt to undertake a comprehensive review: to describe the basic characteristics of DCFH(2); to discuss the present views of the mechanisms of its fluorescence formation; to summarize the fluorescence formation interferents; to outline its application in biological research; and to underline its advantages and disadvantages in ROS detection as well as for the methodological considerations that arise during analysis.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Chen X,Zhong Z,Xu Z,Chen L,Wang Ydoi
10.3109/10715761003709802subject
Has Abstractpub_date
2010-06-01 00:00:00pages
587-604issue
6eissn
1071-5762issn
1029-2470journal_volume
44pub_type
杂志文章,评审abstract::We have here investigated possible occurrence of bicarbonate-dependent, carbonate radical anion (CO(3)(•-))-driven tocopherol-mediated human LDL peroxidation (TMP) in vitro and in vivo. CO(3)(•-), generated in vitro by the SOD1/H(2)O(2)/bicarbonate system, readily promoted TMP, which was dependent on α-tocopherol and ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.719613
更新日期:2012-11-01 00:00:00
abstract:BACKGROUND:The objective of the present study was to determine whether single administration of the antioxidant enzyme bovine superoxide dismutase (bSOD) after radiation therapy (RT) mitigates development of pulmonary toxicity in rats. METHODS:Female F344 rats (n = 60) were divided among six experimental groups: (1) R...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1066501
更新日期:2015-10-01 00:00:00
abstract::Levels of certain antioxidants namely reduced glutathione (GSH), ascorbic acid (Vit C), alpha-tocopherol (Vit E) and antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) were compared in monkey and rat brain microvessels which constitute the blood...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509145642
更新日期:1995-04-01 00:00:00
abstract::Liver microsomal functions related to xenobiotic biotransformation and free radical production were studied in control rats and in animals subjected to L-3,3',5-triiodothyronine (T3) and/or lindane administration as possible mechanisms contributing to oxidative stress, in relation to the activity of enzymes (superoxid...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300051
更新日期:1998-07-01 00:00:00
abstract::Curcumin is a polyphenol derived from the plant Curcuma longa that induces apoptotic cell death in malignant cancer cell lines. It has been shown previously that mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) plays an essential role in defense against oxidative stress by supplying NADPH for antioxidan...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2010.540574
更新日期:2011-04-01 00:00:00
abstract::Of all the aerobic respiration by-products, cytotoxic superoxide derived from mitochondrial-leaked electrons, is the only one known to be disposed of intracellularly. Is this fate the only destiny for mitochondrial-leaked electrons? When Cynomolgus monkeys were injected intravenously with reactive oxygen species (ROS)...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1677902
更新日期:2019-12-01 00:00:00
abstract::The review deals with impairment of Ca(2+)-ATPases by high glucose or its derivatives in vitro, as well as in human diabetes and experimental animal models. Acute increases in glucose level strongly correlate with oxidative stress. Dysfunction of Ca(2+)-ATPases in diabetic and in some cases even in nondiabetic conditi...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2013.807923
更新日期:2013-08-01 00:00:00
abstract::The antioxidative activities of hamamelitannin (2',5-di-O-galloyl-hamamelose), gallic acid and dl-alpha-tocopherol against lipid peroxyl radicals were evaluated in chemical and biological systems. The peroxyl radical scavenging activity was evaluated by electron spin resonance (ESR) method in which both spin-trapping ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509147550
更新日期:1995-05-01 00:00:00
abstract::The classical functions of p53 protein are those related to its role on DNA damage, cell growth arrest, senescence and apoptosis. For this reason it is called 'the guardian of the genome' and is considered one of the most important players in the development of cancer. However, more recently it has been show that p53 ...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2011.571685
更新日期:2011-06-01 00:00:00
abstract::The capacity of non-illuminated nephrotoxin orellanine ([2,2'-bipyridine]-3,3',4,4'-tetrol-1,1'-dioxide) to induce DNA damage in the presence of ferrous iron and dioxygen has been evaluated. Maximal single-strand breaks in plasmid DNA were obtained with a metal to ligand ratio 1:3. Instantaneous oxidation of Fe2+ in p...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760000300681
更新日期:2000-08-01 00:00:00
abstract::Ceruloplasmin (CP) is a blue copper glycoprotein with multiple physiological functions including ferroxidase and oxidase activities. CP is also an important serum oxygen free radical (OFR) scavenger and antioxidant, exerting cardioprotective and antifibrillatory actions. Although it has been reported that CP activitie...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001624079
更新日期:2004-01-01 00:00:00
abstract::Adriamycin (ADR) is nephrotoxic. One component of ADR-induced nephropathy may be oxidative stress. This study used a recently developed line of transgenic mice (Nmt) on the FVB background strain, which over-express the antioxidant protein metallothionein (MT) in podocytes. Cultured podocytes from Nmt mice were resista...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760802657308
更新日期:2009-02-01 00:00:00
abstract::Over the last decades, oxidative stress has been described as a deleterious phenomenon contributing to numerous noncommunicable diseases such as cardiovascular disease, diabetes, and cancers. As many authors ascribed the healthy effect of fruit and vegetable consumption mainly to their antioxidant contents, it has bee...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.853877
更新日期:2013-12-01 00:00:00
abstract::This study evaluated the gender differences in response to intense exercise on lymphocyte hydrogen peroxide production, nitric oxide handling and mitochondrial superoxide dismutase (MnSOD) activity and gene expression. Fifteen males and nine females participated voluntarily in the study and performed a swimming sessio...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760801989926
更新日期:2008-04-01 00:00:00
abstract::Glucose at pathophysiological concentrations was able to accelerate copper-induced oxidation of isolated low-density lipoprotein (LDL) and whole serum. The efficiency of glucose was favored under the following circumstances: (a) when LDL oxidation was induced by low copper concentration, (b) when LDL was partly oxidiz...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760290025906
更新日期:2002-05-01 00:00:00
abstract::Indoles are very common in the body and diet and participate in many biochemical processes. A total of twenty-nine indoles and analogs were examined for their properties as antioxidants and radical scavengers against 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) ABTS*+ radical cation. With only a few exception...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10611860310001648167
更新日期:2004-03-01 00:00:00
abstract::Over the last decade, a dual character of cell response to oxidative stress, eustress versus distress, has become increasingly recognized. A growing body of evidence indicates that under physiological conditions, low concentrations of reactive oxygen and nitrogen species (RONS) maintained by the activity of endogenous...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2018.1457217
更新日期:2018-05-01 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) is a lung disease caused by an inflammatory response to various inhaled toxins, especially cigarette smoke. Reactive oxygen species (ROS) and epigenetic abnormality are intimately related to the pathology of COPD, and the overproduction of ROS results in a decrease of histo...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1696965
更新日期:2019-12-06 00:00:00
abstract::Oxygen radical generating systems, namely, Cu(II)/ H2O2, Cu(II)/ascorbate, Cu(II)/NAD(P)H, Cu(II)/ H2O2/catecholamine and Cu(II)/H2O2/SH-compounds irreversibly inhibited yeast glutathione reductase (GR) but Cu(II)/H2O2 enhanced the enzyme diaphorase activity. The time course of GR inactivation by Cu(II)/H2O2 dependent...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715769709097858
更新日期:1997-12-01 00:00:00
abstract::It has been reported that haem oxygenase-1 (Hmox1) induction attenuates neointimal thickening. Here we further investigated the potential mechanisms regulating this important pathological process. We revealed that histone deacetylase 2 (HDAC2) was induced following Hmox1 induction under 1% oxygen treatment and this in...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1524578
更新日期:2018-10-01 00:00:00
abstract::G72 has been characterised as a susceptibility gene that can have wide-ranging effects in a number of neurodegenerative diseases, including schizophrenia and major depression. Indeed, its product, pLG72, is a potential serum biomarker for schizophrenia. Previous transcriptomic and biochemical studies have indicated th...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1504293
更新日期:2018-09-01 00:00:00
abstract::The effects of various free radicals derived from 6-formylpterin (6-FP), alpha-phenyl-tert-butyl nitrone (PBN) and 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDNA microarrays c...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600946432
更新日期:2007-01-01 00:00:00
abstract::The preventive effect of 20(S)-ginsenoside Rg(3) (20(S)-Rg(3)) on lipopolysaccharide (LPS)-induced oxidative tissue injury in rats was investigated in this study. The elevated serum nitrite/nitrate, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and creatinine levels in LPS-treated control rats were ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701581740
更新日期:2007-10-01 00:00:00
abstract::Oxidatively induced damage caused by free radicals and other DNA-damaging agents generate a plethora of products in the DNA of living organisms. There is mounting evidence for the involvement of this type of damage in the etiology of numerous diseases including carcinogenesis. For a thorough understanding of the mecha...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2015.1014814
更新日期:2015-05-01 00:00:00
abstract::A range of catechins and oligomeric procyanidins was purified by high performance liquid chromatography (HPLC) from grape seed, apple skin, lentil and almond flesh. Catechins, galloylated epicatechin, glycosylated catechin, procyanidin dimers, galloylated dimers, trimer, and tetramer species were all identified, purif...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300391
更新日期:1998-10-01 00:00:00
abstract::Nerve growth factor (NGF) is one of the several structurally related proteins, named neurotrophins (NTs), that regulate neuronal survival, development, function, and plasticity. Moreover, NGF is an important activator of antioxidant mechanisms. These NGF functions are mediated by tropomyosin-related kinase receptor A ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.783210
更新日期:2013-05-01 00:00:00
abstract::Global gene expression profiles of livers from mice, fed diets differing in alpha-tocopherol content, were compared using DNA microarray technology. Three hundred and eighty nine genes were found to significantly differ in their expression level by a factor of 2 or higher between the high and the low alpha-tocopherol ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701416988
更新日期:2007-08-01 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) is a major cause of mortality that has been associated with inflammation and oxidative stress. The purpose of the present case-control study was to determine the relationships between oxidative stress-related genetic variants and the risk and severity of COPD, as well as, t...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1572888
更新日期:2019-03-01 00:00:00
abstract::Human serum samples were evaluated for oxidative stress with the d-ROMs test. The ceruloplasmin (CP) and copper contents of the samples was independently measured and compared to those calculated on the basis of the d-ROMs test results for pure CP solutions. The d-ROMs readings did not show any significant correlation...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1136063
更新日期:2016-01-01 00:00:00
abstract::Orellanine is the tetrahydroxylated and di-N-oxidized bipyridine toxin extracted from several Cortinarius mushrooms among them C. orellanus. The pathogenic mechanism involved in the C. orellanus-poisoning by orellanine leading to kidney impairment is not yet fully understood until now. Electron spin resonance (ESR) sp...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769809066887
更新日期:1998-05-01 00:00:00