Abstract:
:We have here investigated possible occurrence of bicarbonate-dependent, carbonate radical anion (CO(3)(•-))-driven tocopherol-mediated human LDL peroxidation (TMP) in vitro and in vivo. CO(3)(•-), generated in vitro by the SOD1/H(2)O(2)/bicarbonate system, readily promoted TMP, which was dependent on α-tocopherol and bicarbonate concentrations, and was inhibited by the CO(3)(•-) scavenger ethanol; moreover, TMP induced in vitro by the SOD1/H(2)O(2)/bicarbonate system occurred in the presence of α-tocopherol that typically underwent slow oxidative consumption. In the in vivo clinical setting, we showed that, compared to controls, hypertensive patients with diuretic-induced metabolic alkalosis and heightened blood bicarbonate concentration had lipid hydroperoxide burden and decreased α-tocopherol content in the LDL fraction, with direct significant correlation between the LDL levels of α-tocopherol and those of lipid hydroperoxides; remarkably, after resolution of metabolic alkalosis, together with normalization of blood bicarbonate concentration, the LDL content of lipid hydroperoxides was decreased and that of α-tocopherol augmented significantly. These findings suggest bicarbonate-dependent, CO(3)(•-)-driven LDL TMP in vivo. In conclusion, the present study highlights the occurrence of bicarbonate-dependent, CO(3)(•-)-driven human LDL TMP, the role of which in pathological conditions such as atherosclerosis warrants, however, further investigation.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Lapenna D,Ciofani G,Cuccurullo C,Neri M,Giamberardino MA,Cuccurullo Fdoi
10.3109/10715762.2012.719613subject
Has Abstractpub_date
2012-11-01 00:00:00pages
1387-92issue
11eissn
1071-5762issn
1029-2470journal_volume
46pub_type
杂志文章abstract::Manganese superoxide dismutase (MnSOD) levels have been found to be low in human pancreatic cancer [Pancreas 26, (2003), 23] and human pancreatic cancer cell lines [Cancer Res. 63, (2003), 1297] when compared to normal human pancreas. We hypothesized that stable overexpression of pancreatic cancer cells with MnSOD cDN...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400017376
更新日期:2004-11-01 00:00:00
abstract::The hydroxyl radical adducts of 5,5 dimethyl-1-pyrolline-N-oxide (DMPO) and 3,3,5,5 tetramethyl-1-pyrolline-N-oxide (TMPO) formed in the presence of hydrogen peroxide and FeII are normally quite stable, but in the presence of 5-20 micromolar myoglobin their ESR signals decay rapidly. This decay probably reflects furth...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769409145632
更新日期:1994-05-01 00:00:00
abstract::Of all the aerobic respiration by-products, cytotoxic superoxide derived from mitochondrial-leaked electrons, is the only one known to be disposed of intracellularly. Is this fate the only destiny for mitochondrial-leaked electrons? When Cynomolgus monkeys were injected intravenously with reactive oxygen species (ROS)...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1677902
更新日期:2019-12-01 00:00:00
abstract::Septic shock, a severe form of sepsis, is characterized by cardiovascular collapse following microbial invasion of the body. The progressive hypotension, hyporeactivity to vasopressor agents and vascular leak leads to circulatory failure with multiple organ dysfunction and death. Many inflammatory mediators (e.g. TNF-...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760410001725517
更新日期:2004-11-01 00:00:00
abstract::The ability of a range of dietary flavonoids to inhibit low-density lipoprotein (LDL) oxidation in vitro was tested using a number of different methods to assess oxidative damage to LDL. Overall quercetin was the most effective inhibitor of oxidative damage to LDL in vitro. On this basis, a diet enriched with onions a...
journal_title:Free radical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/10715760000300951
更新日期:2000-10-01 00:00:00
abstract::We describe an altered membrane band 3 protein-mediated anion transport in erythrocytes exposed to peroxynitrite, and relate the loss of anion transport to cell damage and to band 3 oxidative modifications. We found that peroxynitrite down-regulate anion transport in a dose dependent relation (100-300 micromoles/l). H...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760601090305
更新日期:2007-03-01 00:00:00
abstract::The effects of inorganic phosphate (Pi), the main intracellular membrane permeable anion capable of altering mitochondrial pH gradients (Delta pH), were measured on mitochondrial H2O2 release. As expected, Pi decreased Delta pH and increased the electric membrane potential (Delta Psi). Mitochondrial H2O2 release was s...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400009258
更新日期:2004-10-01 00:00:00
abstract::Reactive oxygen species (ROS) are critically important chemical intermediates in biological studies, due to their multiple physiologically essential functions and their often pathologically deleterious effects. Consequently, it is vital that their presence in biological samples has to be quantifiable. However, their h...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715761003709802
更新日期:2010-06-01 00:00:00
abstract::Nerve growth factor (NGF) is one of the several structurally related proteins, named neurotrophins (NTs), that regulate neuronal survival, development, function, and plasticity. Moreover, NGF is an important activator of antioxidant mechanisms. These NGF functions are mediated by tropomyosin-related kinase receptor A ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.783210
更新日期:2013-05-01 00:00:00
abstract::Heme oxygenase is the rate limiting enzyme in heme degradation to carbon monoxide (CO), iron and bilirubin. The inducible isoform of the protein, heme oxygenase-1 (HO-1), is susceptible to up-regulation by a diverse variety of conditions and agents in mammalian tissue, leading to the common conception that HO-1 is a s...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715769900301031
更新日期:1999-12-01 00:00:00
abstract::The seasonal variation of CuCl2-mediated low density lipoprotein (LDL) oxidation (10 microM Cu2+, lag phase, rate of oxidation and maximum absorbance at 234 nm) were measured in 43 men and women on 4-6 occasions (mean 5.7 +/- 0.5) over a 12-month period. The lag phase averaged 52.7 +/- 0.6 min and did not differ by ge...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576031000061020
更新日期:2003-03-01 00:00:00
abstract::Multiple acyl-CoA dehydrogenase deficiency (MADD), an autosomal recessive metabolic disorder of fatty acid metabolism, is mostly caused by mutations in the ETFA, ETFB or ETFDH genes that result in dysfunctions in electron transfer flavoprotein (ETF) or electron transfer flavoprotein-ubiquinone dehydrogenase (ETFDH). I...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1500695
更新日期:2018-12-01 00:00:00
abstract::Methylmercury (MeHg) is one of the ubiquitous environmental toxicant that leads to long-lasting neurological deficits in animals and humans. However, the mechanisms of MeHg-induced neuronal cell death are incompletely understood. Treatment of neuronal cells with MeHg (0-2 μM) for 0.5-12 h, or pretreated with LA (12.5-...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2016.1152362
更新日期:2016-01-01 00:00:00
abstract::Oxidative reactions are thought to play a role in the inflammatory condition called fatty liver. It is unclear whether oxidized lipoprotein lipids or proteins are associated with future fatty liver. In the Cardiovascular Risk in Young Finns Study, we determined the circulating levels of LDL and HDL oxidized lipids and...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1133906
更新日期:2016-01-01 00:00:00
abstract::Oxidative stress is a major pathogenetic event occurring in several liver disorders and is a major cause of liver damage due to Ischemia/Reperfusion (I/R) during liver transplantation. While several markers of chronic oxidative stress are well known, early protein targets of oxidative injury are not well defined. In o...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400029603
更新日期:2005-03-01 00:00:00
abstract::Oxidatively generated damage to nucleic acids may play an important role in the pathophysiological processes of a variety of diseases. 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) are oxidatively generated products of DNA and RNA, respectively. Our previous studies have s...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1346249
更新日期:2017-06-01 00:00:00
abstract::The objective of this study was to elucidate the impact of physical activity during the growth period as well as on oxidative stress and antioxidative potential in adulthood. The experimental animals used were four-week old male Wistar rats, which were randomly divided into three groups. The exercise loads were as fol...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1291939
更新日期:2017-02-01 00:00:00
abstract::In aerobes, oxygen is essential for maintenance of life. However, incomplete reduction of oxygen leads to generation of reactive oxygen species. These oxidants oxidise biological macromolecules present in their vicinity and thereby impair cellular functions causing oxidative stress (OS). Aerobes have evolved both enzy...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2019.1702656
更新日期:2020-01-01 00:00:00
abstract::Ischemia-reperfusion injury (IRI) is a leading cause of acute kidney injury (AKI), which contributes to the development of chronic kidney disease (CKD). IRI-induced AKI releases proinflammatory cytokines (e.g. IL-1β, TNF-α, IL-6) that induce a systemic inflammatory response, resulting in proinflammatory cells recruitm...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1668936
更新日期:2019-10-01 00:00:00
abstract::APE1/Ref-1, the mammalian ortholog of E. coli Xth, and a multifunctional protein possessing both DNA repair and transcriptional regulatory activities, has dual role in controlling cellular response to oxidative stress. It is rate-limiting in repair of oxidative DNA damage including strand breaks and also has co-transc...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701765616
更新日期:2008-01-01 00:00:00
abstract::Hydroxyl radical (*OH) generation in the kidney of mice treated with ferric nitrilotriacetate (Fe-NTA) or potassium bromate (KBrO3) in vivo was estimated by the salicylate hydroxylation method, using the optimal experimental conditions we recently reported. Induction of DNA lesions and lipid peroxidation in the kidney...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701644019
更新日期:2007-11-01 00:00:00
abstract::Penicillins, as bactericidal antibiotics, have been widely used to treat infections for several decades. Their structure contains both aromatic and thioether moieties susceptible to free radical oxidation. The (•)OH induced oxidation mechanism of amoxicillin was investigated by pulse radiolysis techniques and by final...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1100729
更新日期:2016-01-01 00:00:00
abstract::Intracellular production of reactive oxygen species (ROS) plays an important role in the control of cell physiology. For the assessment of intracellular ROS production, a plethora of fluorescent probes is commonly used. Interestingly, chemical structures of these probes imply they could be substrates of plasma membran...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2011.579120
更新日期:2011-07-01 00:00:00
abstract::Liver microsomal functions related to xenobiotic biotransformation and free radical production were studied in control rats and in animals subjected to L-3,3',5-triiodothyronine (T3) and/or lindane administration as possible mechanisms contributing to oxidative stress, in relation to the activity of enzymes (superoxid...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300051
更新日期:1998-07-01 00:00:00
abstract::Exposure of dry powder forms of the drugs nitrendipine, nifedipine, felodipine, and nimodipine to gamma-radiation results in the formation of free radicals detected by electron paramagnetic resonance (EPR) spectroscopy. The four structurally related drugs show qualitatively identical EPR spectral features in terms of ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769900300791
更新日期:1999-09-01 00:00:00
abstract::Guanidinosuccinic acid (GSA) is noted for its nitric oxide (NO) mimicking actions such as vasodilatation and activation of the N-methyl-D-aspartate (NMDA) receptor. We have reported that GSA is the product of argininosuccinate (ASA) and some reactive oxygen species, mainly the hydroxyl radical. We tested for GSA synth...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769900300601
更新日期:1999-07-01 00:00:00
abstract::G72 has been characterised as a susceptibility gene that can have wide-ranging effects in a number of neurodegenerative diseases, including schizophrenia and major depression. Indeed, its product, pLG72, is a potential serum biomarker for schizophrenia. Previous transcriptomic and biochemical studies have indicated th...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1504293
更新日期:2018-09-01 00:00:00
abstract::The translocation of transcription factor EB (TFEB) to the nucleus plays a pivotal role in the regulation of basic cellular processes, such as lysosome biogenesis and autophagy. Autophagy is an intracellular degradation system that delivers cytoplasmic constituents to the lysosome, which is important in maintaining ce...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2020.1791843
更新日期:2020-07-28 00:00:00
abstract::Phospholipids (PLs) are important biomolecules that not only constitute structural building blocks and scaffolds of cell and organelle membranes but also play a vital role in cell biochemistry and physiology. Moreover, dietary exogenous PLs are characterised by high nutritional value and other beneficial health effect...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2019.1657573
更新日期:2019-01-01 00:00:00
abstract::Reperfusion injury of the liver occurs in liver transplantation and in major hepatectomies. It triggers a severe oxidative stress that leads to increased lipid peroxidation. In our study we examined the effect of parenteral supranutritional administration of alpha-tocopherol, a vitamin that plays a key role in the end...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300641
更新日期:2001-08-01 00:00:00