Abstract:
:Schistosoma mansoni eggs produced by adult worms in the mesenteric vasculature become trapped in the liver, where they induce granulomatous lesions and strong immune responses. Infected individuals suffer from intestinal schistosomiasis (INT) in 90% of cases, whereas the remaining 10% present with severe hepatosplenic schistosomiasis (HS). The CBA/J mouse model mimics human disease, with 20% of infected mice developing hypersplenomegaly syndrome (HSS) that resembles HS and 80% developing moderate splenomegaly syndrome (MSS) similar to INT. We studied differential patterns of protein expression in livers of 20-week-infected CBA/J mice with MSS or HSS to understand the molecular changes that underlie these two disease forms. Using differential in-gel electrophoresis to identify differentially expressed protein spots, we found 80 protein spots significantly changed with infection and 35 changes specific to severe disease. In particular, the abundances of prohibitin 2, transferrin isoforms, and major urinary protein isoforms were significantly altered in HSS mice. Furthermore, annexin 5, glutathione S-transferase pi class, and S. mansoni phosphoenolpyruvate carboxykinase expression levels changed significantly with schistosome infection. Additionally, levels of major urinary protein decreased and levels of transferrin increased significantly in the sera of HSS mice compared to levels in sera of MSS or control mice, and these differences correlated to the degree of splenomegaly. These findings indicate that the liver protein abundances differ between MSS and HSS mice and may be used for the development of diagnostic markers for the early detection of hepatosplenic schistosomiasis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Manivannan B,Rawson P,Jordan TW,Secor WE,La Flamme ACdoi
10.1128/IAI.00647-09subject
Has Abstractpub_date
2010-02-01 00:00:00pages
618-28issue
2eissn
0019-9567issn
1098-5522pii
IAI.00647-09journal_volume
78pub_type
杂志文章abstract::Many pathogenic Escherichia coli produce the toxin alpha-hemolysin (Hly), and lipopolysaccharide (LPS), interleukin-1 (IL-1), and tumor necrosis factor (TNF) have all been recognized as important effector molecules during infections by gram-negative organisms. Despite the characterization of many in vitro effects of h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
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abstract::A hemagglutinin with an M(r) of 67,000 (pMGA) from Mycoplasma gallisepticum S6 was purified by using monoclonal antibody affinity chromatography. Purified pMGA was treated with a number of enzymes, the resultant peptides were purified, and their amino acid sequence was determined by using an Applied Biosystems (model ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.3.903-909.1993
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1806-1811.1998
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.8.1792-1800.1987
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.2690-2697.2005
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.348-354.1979
更新日期:1979-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00218-17
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00551-13
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2560-2566.1991
更新日期:1991-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.7.1558-1563.1987
更新日期:1987-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5530-5538.2000
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.485-490.1992
更新日期:1992-02-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.74.3.1989-1993.2006
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5508-5513.1999
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abstract::Protection against infection with virulent blood (trypomastigote) forms of Trypanosoma cruzi was accomplished in mice by immunization with culture (mainly epimastigote) forms killed by treatment with sodium perchlorate. Sodium chloride, used instead of sodium perchlorate, with all other conditions kept the same, faile...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.3.461-465.1975
更新日期:1975-09-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4242-4247.2001
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abstract::The mechanisms linking the immune response to cutaneous and mucosal leishmaniasis (CL and ML, respectively) lesions and the response to treatment are incompletely understood. Our aims were to prospectively assess, by quantitative reverse transcription-PCR, the levels of mRNA for gamma interferon, tumor necrosis factor...
journal_title:Infection and immunity
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doi:10.1128/IAI.01513-08
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.3994-4002.1995
更新日期:1995-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.518-521.1984
更新日期:1984-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.3097-3105.1989
更新日期:1989-10-01 00:00:00
abstract::Antibody to Pseudomonas aeruginosa exotoxin was detected in human sera by using a cytotoxicity-neutralization assay. Serum antitoxin was present in high titer in all 14 patients who recovered from serious pseudomonas infections (log2 of 50% neutralization titer, mean +/- standard deviation = 6.0 +/- 1.2). In contrast,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.4.942-947.1976
更新日期:1976-10-01 00:00:00
abstract::Endogenous interleukin-12 (IL-12) mediates protection against Yersinia enterocolitica in C57BL/6 mice by triggering gamma interferon (IFN-gamma) production in NK and CD4+ T cells. Administration of exogenous IL-12 confers protection against yersiniae in Yersinia-susceptible BALB/c mice but exacerbates yersiniosis in r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2213-2220.1998
更新日期:1998-05-01 00:00:00