Abstract:
OBJECTIVE:To purify human muscle-derived cells (hMDCs) that could be used for managing urinary incontinence, erectile dysfunction and for bladder reconstitution. MATERIALS AND METHODS:hMDCs isolated by a modified preplate technique (MPT) from skeletal muscles of limbs were purified by CD34 antibody and magnetic Dynabeads cell selection system (MDCSS). The growth-doubling time of the hMDCs and purified hMDCs was measured. The purified hMDCs were characterized by flow cytometry, immunofluorescence and confocal laser scanning microscopy. RESULTS:The growth-doubling time of hMDCs was approximately 24 h, which increased to 35 h after purifying using the MDCSS. There were scanty fibroblasts after purification and the MDCSS-purified hMDCs were identified by high expression of stem cell markers and myoblast markers. The expression proportion of the stem cell marker CD34 and myoblast marker CD56 in the hMDCs was higher after purification (MDCSS) than before (MPT), at 32.13% vs 4.12% and 21.56% vs 8.60%, respectively. CONCLUSIONS:The purification of hMDCs showing high expression of stem cell and myoblast markers is feasible. These purified hMDCs could potentially be used for urological regeneration.
journal_name
BJU Intjournal_title
BJU internationalauthors
Lu SH,Yang AH,Chen KK,Chiang HS,Chang LSdoi
10.1111/j.1464-410X.2009.09032.xsubject
Has Abstractpub_date
2010-06-01 00:00:00pages
1598-603issue
11eissn
1464-4096issn
1464-410Xpii
BJU9032journal_volume
105pub_type
杂志文章abstract::To establish a set of consensus statements to facilitate physician management strategies for patients with metastatic prostate cancer (mPCa) in Hong Kong. A local expert consensus was organized jointly by the two main professional organizations representing prostate cancer specialists in Hong Kong. A total of 12 exper...
journal_title:BJU international
pub_type: 共识发展会议,杂志文章
doi:10.1111/bju.14091
更新日期:2018-05-01 00:00:00
abstract:UNLABELLED:What's known on the subject? and What does the study add? There have been few studies evaluating the prognostic value of epithelial-mesenchymal transition markers in renal cell carcinoma (RCC); therefore, the significance of these markers in the prognosis of patients with RCC, particularly that in those with...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2012.11297.x
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVE:To report our experience of 11 children with pelvi-ureteric junction obstruction (PUJO) in a duplex kidney and to review previous reports. PATIENTS AND METHODS:From 1995 to 2005 the second author (G.H.H.S.) performed 145 pyeloplasties in single-system kidneys and in 11 children with PUJO in a duplex kidney. ...
journal_title:BJU international
pub_type: 杂志文章,评审
doi:10.1111/j.1464-410X.2007.07386.x
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:To examine the effect of short-course androgen-suppression therapy (AST) before brachytherapy on all-cause mortality (ACM) rates, stratified by the presence or absence of a history of myocardial infarction (MI) or stroke. AST is used to reduce prostate size to enable men with favourable-risk prostate cancer t...
journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2010.09273.x
更新日期:2010-10-01 00:00:00
abstract:OBJECTIVE:To investigate the effects of a selective type 4 cyclic nucleotide phosphodiesterase (PDE4) inhibitor, IC486051, on bladder activity in normal rats and those with and bladder outlet obstruction (BOO), as inhibition of PDE4 leads to elevation of intracellular cAMP levels and relaxation of smooth muscle. MATER...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2006.06643.x
更新日期:2007-03-01 00:00:00
abstract:OBJECTIVE:To report experience with a minimally invasive technique for palliation of urinary fistula/incontinence complicating advanced pelvic malignancy or its treatment. PATIENTS AND METHODS:We used ureteric embolization with permanent nephrostomy drainage in eight renal units in five patients for palliation of symp...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2006.06522.x
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:To study the natural history of renal cell carcinoma (RCC) development in renal grafts and their management. PATIENTS AND METHODS:We report a single-centre series of de novo RCC in allografts from a cohort of 2396 consecutive renal transplant recipients. RESULTS:In all, 17 RCCs were detected in 12 patients,...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2011.10315.x
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:To determine the incidence and treatment of lymphoceles after retropubic radical prostatectomy (RP). PATIENTS AND METHODS:Up to January 2004, 260 patients who had a retropubic RP in one institution by one surgeon were assessed retrospectively, using the patients' notes or the computerized results system to d...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2005.05398.x
更新日期:2005-04-01 00:00:00
abstract:OBJECTIVE:To assess the rate of prostate-specific antigen (PSA) testing for prostate cancer in general practice in asymptomatic and symptomatic patients. SUBJECTS AND METHODS:The cross-sectional study took place in England and Wales, was population-based and covered 469 159 men aged 45-84 years. Pathology data on PSA ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-4096.2004.04832.x
更新日期:2004-07-01 00:00:00
abstract:OBJECTIVE:To evaluate two methods of reducing the urine output during treatment (the most easily manipulated variable) in patients undergoing intravesical instillation with mitomycin C, where the concentration-time curve also depends upon dose, diluent volume, residual urine volume, and drug absorption and degradation....
journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2000.00869.x
更新日期:2000-10-01 00:00:00
abstract:OBJECTIVE:To compare, in a retrospective observational cohort study, the efficacy, tolerability, safety and clinical effectiveness of propiverine and oxybutynin in children and adolescents with neurogenic detrusor overactivity (NDO). PATIENTS AND METHODS:In all, 255 children and adolescents (aged 1-18 years) with NDO ...
journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2008.08093.x
更新日期:2009-03-01 00:00:00
abstract:UNLABELLED:Study Type - Diagnostic (exploratory cohort) Level of Evidence 2b What's known on the subject? and What does the study add? The widespread use of serum PSA testing followed by TRUS-guided biopsy have resulted in profound prostate cancer stage migration with many patients presenting with focal rather than mul...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2011.10704.x
更新日期:2012-07-01 00:00:00
abstract:OBJECTIVE:To evaluate whether Raman molecular imaging (RMI, which combines digital imaging and analytical spectroscopy to evaluate the biochemical composition of interrogated material) can be used to identify biochemical differences in patients with Gleason 7 prostate cancer who progress to metastatic disease and die f...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2010.09185.x
更新日期:2010-08-01 00:00:00
abstract:OBJECTIVES:To determine the effects of LRIG1 on the growth, migration and invasion of bladder cancer cells and the mechanisms underlying such effects. MATERIALS AND METHODS:The plasmid pLRIG1-green fluorescence protein (GFP) was transfected into BIU87 bladder cancer cells by Lipofectamine2000 (Invitrogen, Groningen, t...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2006.06405.x
更新日期:2006-10-01 00:00:00
abstract:OBJECTIVE:To assess the sensitivity and specificity of blue-light cystoscopy (BLC) with hexaminolevulinate as an adjunct to white-light cystoscopy (WLC) vs WLC alone for the detection of non-muscle-invasive bladder cancer (NMIBC), in routine clinical practice in Spain. PATIENTS AND METHOD:An intra-patient comparative,...
journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/bju.13020
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVE:To compare outcomes of minimally invasive radical nephrectomy (MIS-RN) and robot-assisted partial nephrectomy (RAPN) in clinical T2a renal mass (cT2aRM). PATIENTS AND METHODS:Retrospective, multicentre, propensity score-matched (PSM) comparison of RAPN and MIS-RN for cT2aRM (T2aN0M0). Cohorts were PSM for ag...
journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/bju.15064
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:To present our experience with bladder cancer among a renal transplant population and to review critically the relevant literature. PATIENTS AND METHODS:In all, 1865 renal graft recipients were followed for a mean (SD) of 6.5 (5) years. Seven recipients (all men) developed a urothelial bladder tumour. The s...
journal_title:BJU international
pub_type: 杂志文章,评审
doi:10.1111/j.1464-410X.2007.07210.x
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVES:• To investigate whether cysteine-rich secretory protein 3 (CRISP-3) and/or β-microseminoprotein (β-MSP) expression in diagnostic prostate needle biopsies have predictive value for prostate cancer (PC) on radical prostatecomy (RP). • To evaluate their potential clinical implementation in a preoperative setti...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2010.10059.x
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVE:To study the effects of diuretic stimulation with frusemide and mannitol on the renal resistive index (RI) of the undilated unobstructed porcine kidney. MATERIALS AND METHODS:Experiments were carried out on 14 pigs divided into two equal groups (A and B), under general anaesthesia. In all pigs both ureters w...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-4096.2001.01795.x
更新日期:2002-03-01 00:00:00
abstract:OBJECTIVE:To investigate the immunology of host-tumour interaction, critical for the development of immunotherapy against cancers, by assessing the major histocompatibility complex (MHC) class I expression in both benign and malignant prostate disease, and the relationship between their expression and degree of tumour-...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2002.02993.x
更新日期:2002-11-01 00:00:00
abstract:OBJECTIVE:To investigate the extent to which different types of bladder dysfunction can affect long-term renal function in boys with posterior urethral valves (PUV). PATIENTS AND METHODS:Renal and bladder function were retrospectively assessed in 59 boys with PUV (mean age 10 years, range 5-17). All patients included ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2002.02881.x
更新日期:2002-08-01 00:00:00
abstract:OBJECTIVE:To report our contemporary experience with renal autotransplantation (AT), an established treatment for managing patients with a shortened ureter or renovascular disease, as despite its historical importance, AT remains an underused technique by urologists. PATIENTS AND METHODS:All patients undergoing AT bet...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2005.05729.x
更新日期:2005-10-01 00:00:00
abstract:OBJECTIVE:To explore whether the number of unfavourable pretreatment risk factors predicts cause-specific mortality in men treated with prostate brachytherapy. PATIENTS AND METHODS:Between April 1995 and March 2006, 739 patients were treated who had at least one of the following adverse risk factors: pretreatment pros...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2010.09269.x
更新日期:2010-09-01 00:00:00
abstract:OBJECTIVE:To compare directly the performance of the ADXBLADDER test with that of cytology in the detection of non-muscle-invasive bladder cancer (NMIBC) recurrences. BACKGROUND:ADXBLADDER is a urine test based on the detection of MCM5, a DNA licensing factor expressed in all cells capable of dividing. Expression is u...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.15194
更新日期:2020-08-03 00:00:00
abstract:UNLABELLED:Authors from Iowa City report on the incidence of RCC; they compared the rate of these tumours at autopsy and felt that the decrease found was a result of better antemortem detection, and an increase with time in the frequency of clinically detected renal cancer. A study from New York attempted to determine ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2005.05244.x
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVE:To report our experience of using laparoscopy for the accurate location and simultaneous removal of small dysplastic kidneys with ectopic ureteric insertion causing urinary incontinence and that were not detected by conventional imaging modalities. PATIENTS AND METHODS:Seven girls (mean age 7.9 years, range ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.1999.00162.x
更新日期:1999-08-01 00:00:00
abstract:UNLABELLED:What's known on the subject? and what does the study add?: Only little and partly contradictory data are currently published about the prognostic role of immunohistochemically detectable proliferation-associated biomarkers in surgically treated squamous cell carcinoma of the penis (SCCP), and no data are ava...
journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2012.11735.x
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVE:To determine the changes in body composition, muscle function and energy expenditure after radical cystectomy (RC). PATIENTS AND METHODS:Eleven consecutive men (median age 66 years, range 44-79) who had a RC over a 1-year period had measurements made of their total body protein, by neutron-activation analysi...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2007.07337.x
更新日期:2008-04-01 00:00:00
abstract:OBJECTIVE:To investigate the utility of a new 'modified-prone' position for treating pre-vesical stones with extracorporeal shock wave lithotripsy (ESWL), usually considered an acceptable and effective treatment for such stones, but for which many different body positions have been used in an attempt to increase its ef...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410x.2003.04619.x
更新日期:2004-02-01 00:00:00
abstract:OBJECTIVE:To determine the relative prevalence of various definitions of microscopic haematuria (MH) in patients with renal neoplasms and controls, and to predict the likely outcome of renal imaging for those definitions. PATIENTS AND METHODS:In a retrospective case-control study 278 adult men and woman seen between 1...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2005.05694.x
更新日期:2005-09-01 00:00:00