Abstract:
:In type 2 diabetes, a strong correlation between intramyocellular lipid accumulation and insulin resistance exists but whether intramyocellular accumulation is a cause or a consequence of insulin resistance is not clear. Lipid accumulation and response to insulin were evaluated in primary human myotubes derived from non-diabetic subjects and type 2 diabetic patients. Myotubes derived from type 2 diabetic patients had a defective response to insulin without showing a significant increase in lipid accumulation compared to myotubes derived from non-diabetic subjects. In myotubes derived from non-diabetic subjects, response to insulin stimulation (Akt phosphorylation) was abrogated and lipid content was increased after palmitate treatment. However, chronic exposure to insulin or inhibition of mitochondrial activity by antimycin led to independent changes of lipid content and response to insulin in myotubes derived from non-diabetic subjects. Altogether these results suggest that lipid accumulation and response to insulin are not invariably linked.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Aguer C,Mercier J,Kitzmann Mdoi
10.1016/j.mce.2009.10.017subject
Has Abstractpub_date
2010-02-05 00:00:00pages
225-32issue
1-2eissn
0303-7207issn
1872-8057pii
S0303-7207(09)00564-4journal_volume
315pub_type
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