Abstract：
:Genetic conservation allows ancient features of fat storage endocrine pathways to be explored in C. elegans. Multiple studies have used Nile red or BODIPY-labeled fatty acids to identify regulators of fat mass. When mixed with their food, E. coli bacteria, Nile red, and BODIPY-labeled fatty acids stain multiple spherical cellular structures in the C. elegans major fat storage organ, the intestine. However, here we demonstrate that, in the conditions previously reported, the lysosome-related organelles stained by Nile red and BODIPY-labeled fatty acids are not the C. elegans major fat storage compartment. We show that the major fat stores are contained in a distinct cellular compartment that is not stained by Nile red. Using biochemical assays, we validate oil red O staining as a method to assess major fat stores in C. elegans, allowing for efficient and accurate genetic and functional genomic screens for genes that control fat accumulation at the organismal level.
journal_name
Cell Metabjournal_title
Cell metabolismauthors
O'Rourke EJ,Soukas AA,Carr CE,Ruvkun Gdoi
10.1016/j.cmet.2009.10.002subject
Has Abstractpub_date
2009-11-01 00:00:00pages
430-5issue
5eissn
1550-4131issn
1932-7420pii
S1550-4131(09)00301-5journal_volume
10pub_type
杂志文章相关文献
Cell Metabolism文献大全abstract：:Myriad strategies have been explored to compensate for the lack of dystrophin or to skip mutations that cause the lethal disease Duchenne muscular dystrophy (DMD). A new study shows that gene editing strategies used by bacteria can be applied in zygotes of a mouse model of DMD to correct the genetic defect that causes...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2014.11.011
更新日期：2014-12-02 00:00:00
abstract：:VEGF (vascular endothelial growth factor) signaling inhibitors are widely used in different cancer types; however, patient selection remains a challenge. Analyses of samples from a phase III clinical trial in metastatic colorectal cancer testing chemotherapy versus chemotherapy with the small molecule VEGF receptors i...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2015.10.015
更新日期：2016-01-12 00:00:00
abstract：:Transgenerational inheritance of epigenetic characteristics in plants has been reported, whereas nongenetic persistence of complex phenotypes in animals is controversial. A recent report by Anne Brunet and colleagues describes a fascinating example of persistence across generations of extended life span in worm and ex...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2011.12.012
更新日期：2012-01-04 00:00:00
abstract：:Several lines of evidence suggest that mitochondrial dysfunction plays a critical role in the pathogenesis of microvascular complications of diabetes, including diabetic nephropathy. However, the signaling pathways by which hyperglycemia leads to mitochondrial dysfunction are not fully understood. Here we examined the...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2012.01.009
更新日期：2012-02-08 00:00:00
abstract：:Metabolomics is the systematic identification and quantitation of all metabolites in a given organism or biological sample. The enhanced resolution provided by nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS), along with powerful chemometric software, allows the simultaneous determination and c...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2007.10.005
更新日期：2007-11-01 00:00:00
abstract：:Evolution favored individuals with superior cognitive and physical abilities under conditions of limited food sources, and brain function can therefore be optimized by intermittent dietary energy restriction (ER) and exercise. Such energetic challenges engage adaptive cellular stress-response signaling pathways in neu...
journal_title：Cell metabolism
pub_type： 杂志文章,评审
doi：10.1016/j.cmet.2012.08.012
更新日期：2012-12-05 00:00:00
abstract：:Metformin use is associated with reduced cancer mortality, but how metformin impacts cancer outcomes is controversial. Although metformin can act on cells autonomously to inhibit tumor growth, the doses of metformin that inhibit proliferation in tissue culture are much higher than what has been described in vivo. Here...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2016.09.006
更新日期：2016-11-08 00:00:00
abstract：:In this issue of the Cell Metabolism, Chevalier et al. show that a warm environment produces changes in the composition of intestinal microbiota and that these changes can prevent bone loss due to hypogonadism. Dovetailing with prior studies on the ability of probiotics to reverse hypogonadism-induced osteopenia, the ...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2020.09.010
更新日期：2020-10-06 00:00:00
abstract：:PTPMT1 was the first protein tyrosine phosphatase found localized to the mitochondria, but its biological function was unknown. Herein, we demonstrate that whole body deletion of Ptpmt1 in mice leads to embryonic lethality, suggesting an indispensable role for PTPMT1 during development. Ptpmt1 deficiency in mouse embr...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2011.04.007
更新日期：2011-06-08 00:00:00
abstract：:Efficient insulin secretion requires a well-functioning pancreatic islet microvasculature. The dense network of islet capillaries includes the islet pericyte, a cell that has barely been studied. Here we show that islet pericytes help control local blood flow by adjusting islet capillary diameter. Islet pericytes cove...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2018.02.016
更新日期：2018-03-06 00:00:00
abstract：:Type 1 diabetes is an autoimmune disease that manifests as impaired insulin secretion, with compounding complications over time. Bhatt et al. (2015) investigate protective mechanisms in survivors of type 1 diabetes by using induced pluripotent stem cells as genetic models, uncovering novel interactions between microRN...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2015.07.018
更新日期：2015-08-04 00:00:00
abstract：:Common variants in WNT pathway genes have been associated with bone mass and fat distribution, the latter predicting diabetes and cardiovascular disease risk. Rare mutations in the WNT co-receptors LRP5 and LRP6 are similarly associated with bone and cardiometabolic disorders. We investigated the role of LRP5 in human...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2015.01.009
更新日期：2015-02-03 00:00:00
abstract：:Peroxisome proliferator-activated receptor δ (PPARδ) is a critical regulator of energy metabolism in the heart. Here, we propose a mechanism that integrates two deleterious characteristics of heart failure, hypoxia and a metabolic shift toward glycolysis, involving the microRNA cluster miR-199a∼214 and PPARδ. We demon...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2013.08.009
更新日期：2013-09-03 00:00:00
abstract：:The PAR-domain basic leucine zipper (PAR bZip) transcription factors DBP, TEF, and HLF accumulate in a highly circadian manner in several peripheral tissues, including liver and kidney. Mice devoid of all three of these proteins are born at expected Mendelian ratios, but are epilepsy prone, age at an accelerated rate,...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2006.04.015
更新日期：2006-07-01 00:00:00
abstract：:The discovery that their neonatal diabetes is caused by gain-of-function mutations in the K(ATP) channel has enabled many patients to switch from insulin to oral sulphonylurea drugs. Here, I review molecular, physiological, and clinical features of this change in therapy. ...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2010.02.004
更新日期：2010-03-03 00:00:00
abstract：:Aging is driven by changes of the epigenetic state that are only partially understood. We performed a comprehensive epigenomic analysis of the pancreatic β cell, key player in glucose homeostasis, in adolescent and very old mice. We observe a global methylation drift resulting in an overall more leveled methylome in o...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2015.07.025
更新日期：2015-10-06 00:00:00
abstract：:In this issue of Cell Metabolism, Macdougall et al. (2018) identify two subsets of conventional dendritic cells in visceral adipose tissue and demonstrate that these subsets engage distinct adipocyte-associated signaling pathways to drive their tolerogenic phenotypes in the lean state. ...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2018.02.014
更新日期：2018-03-06 00:00:00
abstract：:Leptin signals the repletion of fat stores, acting in the CNS to permit energy utilization by a host of autonomic and neuroendocrine processes and to decrease feeding. While much recent research has focused on the leptin-regulated circuitry of the hypothalamic arcuate nucleus (ARC), the majority of brain leptin recept...
journal_title：Cell metabolism
pub_type： 杂志文章,评审
doi：10.1016/j.cmet.2008.12.001
更新日期：2009-02-01 00:00:00
abstract：:Exercise leads to changes in muscle phenotype with important implications for exercise performance and health. A recent paper in Cell by Narkar et al. (2008) shows that many of the adaptations in muscle phenotype elicited by exercise can be mimicked by genetic manipulation and drug treatment in mice. ...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2008.07.004
更新日期：2008-08-01 00:00:00
abstract：:Small-molecule ligands of nuclear hormone receptors (NHRs) govern the transcriptional regulation of metazoan development, cell differentiation, and metabolism. However, the physiological ligands of many NHRs remain poorly characterized, primarily due to lack of robust analytical techniques. Using comparative metabolom...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2013.11.024
更新日期：2014-01-07 00:00:00
abstract：:Microglia play a crucial role in immune responses, including inflammation. Diet-induced obesity (DIO) triggers microglia activation and hypothalamic inflammation as early as 3 days after high-fat diet (HFD) exposure, before changes in body weight occur. The intracellular mechanism(s) responsible for HFD-induced microg...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2019.08.010
更新日期：2019-11-05 00:00:00
abstract：:You are what you eat; but when you eat also seems to be important for a healthy metabolism. In this issue of Cell Metabolism, Benegiamo et al. (2018) uncover a mechanism by which the RNA-binding protein NONO promotes the time-of-day-dependent expression of key metabolic genes at a post-transcriptional level in respons...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2018.01.009
更新日期：2018-02-06 00:00:00
abstract：:We sequenced Drosophila head RNA to identify a small set of miRNAs that undergo robust circadian cycling. We concentrated on a cluster of six miRNAs, mir-959-964, all of which peak at about ZT12 or lights off. The cluster pri-miRNA is transcribed under bona fide circadian transcriptional control, and all six mature mi...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2012.10.002
更新日期：2012-11-07 00:00:00
abstract：:Fibrosis is increasingly appreciated as a major player in adipose tissue dysfunction. In rapidly expanding adipose tissue, pervasive hypoxia leads to an induction of HIF1α that in turn leads to a potent profibrotic transcriptional program. The pathophysiological impact of adipose tissue fibrosis is likely to play an e...
journal_title：Cell metabolism
pub_type： 杂志文章,评审
doi：10.1016/j.cmet.2013.06.016
更新日期：2013-10-01 00:00:00
abstract：:Precise control of the thyroid hormone (T3)-dependent transcriptional program is required by multiple cell systems, including muscle stem cells. Deciphering how this is achieved and how the T3 signal is controlled in stem cell niches is essentially unknown. We report that in response to proliferative stimuli such as a...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2014.10.009
更新日期：2014-12-02 00:00:00
abstract：:Skeletal and cardiac muscle depend on high turnover of ATP made by mitochondria in order to contract efficiently. The transcriptional coactivator PGC-1alpha has been shown to function as a major regulator of mitochondrial biogenesis and respiration in both skeletal and cardiac muscle, but this has been based only on g...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2005.03.002
更新日期：2005-04-01 00:00:00
abstract：:Circadian clocks regulate most aspects of physiology and metabolism. Genome-wide approaches have uncovered widespread circadian rhythms in the transcriptome, cistrome, and epigenome of mice, and now two proteomics studies in this issue (Robles et al., 2016; Wang et al., 2016) reveal extensive circadian regulation of t...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2016.12.014
更新日期：2017-01-10 00:00:00
abstract：:Signaling by the corticotropin-releasing factor receptor type 1 (CRFR1) plays an important role in mediating the autonomic response to stressful challenges. Multiple hypothalamic nuclei regulate sympathetic outflow. Although CRFR1 is highly expressed in the arcuate nucleus (Arc) of the hypothalamus, the identity of th...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2016.04.017
更新日期：2016-06-14 00:00:00
abstract：:Proper control of hepatic glucose production is central to whole-body glucose homeostasis, and its disruption plays a major role in diabetes. Here, we demonstrate that although established as an intracellular lipid chaperone, aP2 is in fact actively secreted from adipocytes to control liver glucose metabolism. Secreti...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2013.04.012
更新日期：2013-05-07 00:00:00
abstract：:Most animal models of obesity and hyperinsulinemia are associated with increased vagal cholinergic activity. The M3 muscarinic acetylcholine receptor subtype is widely expressed in the brain and peripheral tissues and plays a key role in mediating the physiological effects of vagal activation. Here, we tested the hypo...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2006.09.008
更新日期：2006-11-01 00:00:00
