Experimental and computational characterization of biological liquid crystals: a review of single-molecule bioassays.

Abstract:

:Quantitative understanding of the mechanical behavior of biological liquid crystals such as proteins is essential for gaining insight into their biological functions, since some proteins perform notable mechanical functions. Recently, single-molecule experiments have allowed not only the quantitative characterization of the mechanical behavior of proteins such as protein unfolding mechanics, but also the exploration of the free energy landscape for protein folding. In this work, we have reviewed the current state-of-art in single-molecule bioassays that enable quantitative studies on protein unfolding mechanics and/or various molecular interactions. Specifically, single-molecule pulling experiments based on atomic force microscopy (AFM) have been overviewed. In addition, the computational simulations on single-molecule pulling experiments have been reviewed. We have also reviewed the AFM cantilever-based bioassay that provides insight into various molecular interactions. Our review highlights the AFM-based single-molecule bioassay for quantitative characterization of biological liquid crystals such as proteins.

journal_name

Int J Mol Sci

authors

Eom K,Yang J,Park J,Yoon G,Soo Sohn Y,Park S,Yoon DS,Na S,Kwon T

doi

10.3390/ijms10094009

subject

Has Abstract

pub_date

2009-09-10 00:00:00

pages

4009-32

issue

9

issn

1422-0067

journal_volume

10

pub_type

杂志文章,评审
  • Different Sensitivity of Macrophages to Phospholipidosis Induction by Amphiphilic Cationic Drugs.

    abstract::Phospholipidosis (PLD), the intracellular accumulation of phospholipids, is an adaptive response to toxic stimuli and serves as an important parameter in the biological assessment of compounds. Cationic amphiphilic drugs are the main inducers of PLD and may impair the function of alveolar macrophages. In vivo and in v...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21218391

    authors: Öhlinger K,Absenger-Novak M,Meindl C,Ober J,Fröhlich E

    更新日期:2020-11-09 00:00:00

  • Impact of Sarcopenia as a Prognostic Biomarker of Bladder Cancer.

    abstract::Sarcopenia, the degenerative and systemic loss of skeletal muscle mass, indicates patient frailty and impaired physical function. Sarcopenia can be caused by multiple factors, including advanced age, lack of exercise, poor nutritional status, inflammatory diseases, endocrine diseases, and malignancies. In patients wit...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19102999

    authors: Fukushima H,Takemura K,Suzuki H,Koga F

    更新日期:2018-10-01 00:00:00

  • Structural Basis of Oligomerization of N-Terminal Domain of Spider Aciniform Silk Protein.

    abstract::Spider silk is self-assembled from water-soluble silk proteins through changes in the environment, including pH, salt concentrations, and shear force. The N-terminal domains of major and minor ampullate silk proteins have been found to play an important role in the assembly process through salt- and pH-dependent dimer...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21124466

    authors: Chakraborty R,Fan JS,Lai CC,Raghuvamsi PV,Chee PX,Anand GS,Yang D

    更新日期:2020-06-23 00:00:00

  • Mechanistic and Kinetic Study on Self-/Cross- Condensation of PCTA/DT Formation Mechanisms from Three Types of Radicals of 2,4-Dichlorothiophenol.

    abstract::Chlorothiophenols (CTPs) are known to be key and direct precursors of polychlorinated thianthrene/dibenzothiophenes (PCTA/DTs). Self/cross-coupling of the chlorothiophenoxy radicals (CTPRs), sulfydryl-substituted phenyl radicals and thiophenoxyl diradicals evolving from CTPs are initial and important steps for PCTA/DT...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20112623

    authors: Wang H,Zuo C,Zheng S,Sun Y,Xu F,Zhang Q

    更新日期:2019-05-28 00:00:00

  • Parameters for Optoperforation-Induced Killing of Cancer Cells Using Gold Nanoparticles Functionalized With the C-terminal Fragment of Clostridium Perfringens Enterotoxin.

    abstract::Recently, we used a recombinant produced C-terminus (D194-F319) of the Clostridium perfringens enterotoxin (C-CPE) to functionalize gold nanoparticles (AuNPs) for a subsequent specific killing of claudin expressing tumor cells using the gold nanoparticle-mediated laser perforation (GNOME-LP) technique. For a future in...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20174248

    authors: Becker A,Lehrich T,Kalies S,Heisterkamp A,Ngezahayo A

    更新日期:2019-08-30 00:00:00

  • Extracellular Vesicle Membrane-Associated Proteins: Emerging Roles in Tumor Angiogenesis and Anti-Angiogenesis Therapy Resistance.

    abstract::The tumor vasculature is essential for tumor growth and metastasis, and is a prime target of several anti-cancer agents. Increasing evidence indicates that tumor angiogenesis is stimulated by extracellular vesicles (EVs) that are secreted or shed by cancer cells. These EVs encapsulate a variety of biomolecules with an...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21155418

    authors: Ko SY,Naora H

    更新日期:2020-07-30 00:00:00

  • EAAC1 gene deletion increases neuronal death and blood brain barrier disruption after transient cerebral ischemia in female mice.

    abstract::EAAC1 is important in modulating brain ischemic tolerance. Mice lacking EAAC1 exhibit increased susceptibility to neuronal oxidative stress in mice after transient cerebral ischemia. EAAC1 was first described as a glutamate transporter but later recognized to also function as a cysteine transporter in neurons. EAAC1-m...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms151119444

    authors: Choi BY,Kim JH,Kim HJ,Lee BE,Kim IY,Sohn M,Suh SW

    更新日期:2014-10-27 00:00:00

  • The tRNA-dependent biosynthesis of modified cyclic dipeptides.

    abstract::In recent years it has become apparent that aminoacyl-tRNAs are not only crucial components involved in protein biosynthesis, but are also used as substrates and amino acid donors in a variety of other important cellular processes, ranging from bacterial cell wall biosynthesis and lipid modification to protein turnove...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms150814610

    authors: Giessen TW,Marahiel MA

    更新日期:2014-08-21 00:00:00

  • Cannabidiol-from Plant to Human Body: A Promising Bioactive Molecule with Multi-Target Effects in Cancer.

    abstract::Cannabis sativa L. is a plant long used for its textile fibers, seed oil, and oleoresin with medicinal and psychoactive properties. It is the main source of phytocannabinoids, with over 100 compounds detected so far. In recent years, a lot of attention has been given to the main phytochemicals present in Cannabis sati...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20235905

    authors: Kis B,Ifrim FC,Buda V,Avram S,Pavel IZ,Antal D,Paunescu V,Dehelean CA,Ardelean F,Diaconeasa Z,Soica C,Danciu C

    更新日期:2019-11-25 00:00:00

  • Soluble Alpha-Klotho Alleviates Cardiac Fibrosis without Altering Cardiomyocytes Renewal.

    abstract::Heart disease is the leading cause of death worldwide. The major cause of heart failure is the death of the myocardium caused by myocardial infarction, detrimental cardiac remodeling, and cardiac fibrosis occurring after the injury. This study aimed at discovering the role of the anti-aging protein α-klotho (KL), whic...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21062186

    authors: Chen WY

    更新日期:2020-03-22 00:00:00

  • Combined Transcriptomics, Proteomics and Bioinformatics Identify Drug Targets in Spinal Cord Injury.

    abstract::Spinal cord injury (SCI) causes irreversible tissue damage and severe loss of neurological function. Currently, there are no approved treatments and very few therapeutic targets are under investigation. Here, we combined 4 high-throughput transcriptomics and proteomics datasets, 7 days and 8 weeks following clinically...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19051461

    authors: Tica J,Bradbury EJ,Didangelos A

    更新日期:2018-05-14 00:00:00

  • The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins-Metal Complexes.

    abstract::The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behav...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18030610

    authors: Merlos Rodrigo MA,Molina-López J,Jimenez Jimenez AM,Planells Del Pozo E,Adam P,Eckschlager T,Zitka O,Richtera L,Adam V

    更新日期:2017-03-11 00:00:00

  • Role of P2Y Receptors in Platelet Extracellular Vesicle Release.

    abstract::Platelet extracellular vesicles (PEVs) are potential new biomarkers of platelet activation which may allow us to predict and/or diagnose developing coronary thrombosis before myocardial necrosis occurs. The P2Y1 and P2Y12 receptors play a key role in platelet activation and aggregation. Whereas the P2Y1 antagonists ar...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21176065

    authors: Gąsecka A,Rogula S,Eyileten C,Postuła M,Jaguszewski MJ,Kochman J,Mazurek T,Nieuwland R,Filipiak KJ

    更新日期:2020-08-23 00:00:00

  • At-Hook Motif Nuclear Localised Protein 18 as a Novel Modulator of Root System Architecture.

    abstract::The At-Hook Motif Nuclear Localized Protein (AHL) gene family encodes embryophyte-specific nuclear proteins with DNA binding activity. They modulate gene expression and affect various developmental processes in plants. We identify AHL18 (At3G60870) as a developmental modulator of root system architecture and growth. A...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21051886

    authors: Širl M,Šnajdrová T,Gutiérrez-Alanís D,Dubrovsky JG,Vielle-Calzada JP,Kulich I,Soukup A

    更新日期:2020-03-10 00:00:00

  • Detergent Resistant Membrane Domains in Broccoli Plasma Membrane Associated to the Response to Salinity Stress.

    abstract::Detergent-resistant membranes (DRMs) microdomains, or "raft lipids", are key components of the plasma membrane (PM), being involved in membrane trafficking, signal transduction, cell wall metabolism or endocytosis. Proteins imbibed in these domains play important roles in these cellular functions, but there are few st...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21207694

    authors: Yepes-Molina L,Carvajal M,Martínez-Ballesta MC

    更新日期:2020-10-17 00:00:00

  • Orofacial Manifestations and Temporomandibular Disorders of Systemic Scleroderma: An Observational Study.

    abstract::Scleroderma is a disorder involving oral and facial tissues, with skin hardening, thin lips, deep wrinkles, xerostomia, tongue rigidity, and microstomia. The aim of this study was to investigate the prevalence of oral manifestations and temporomandibular disorders (TMD) in Systemic Sclerosis (SSc) patients compared wi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,随机对照试验

    doi:10.3390/ijms17071189

    authors: Crincoli V,Fatone L,Fanelli M,Rotolo RP,Chialà A,Favia G,Lapadula G

    更新日期:2016-07-22 00:00:00

  • Microglial Function across the Spectrum of Age and Gender.

    abstract::Microglia constitute the resident immunocompetent cells of the central nervous system. Although much work has focused on their ability to mount an inflammatory response in reaction to pathology, recent studies have delved into their role in maintaining homeostasis in the healthy brain. It is important to note that the...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18030561

    authors: Nissen JC

    更新日期:2017-03-04 00:00:00

  • A Narrative Role of Vitamin D and Its Receptor: With Current Evidence on the Gastric Tissues.

    abstract::Vitamin D is a major steroid hormone that is gaining attention as a therapeutic molecule. Due to the general awareness of its importance for the overall well-being, vitamin D deficiency (VDD) is now recognized as a major health issue. The main reason for VDD is minimal exposure to sunlight. The vitamin D receptor (VDR...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20153832

    authors: Sirajudeen S,Shah I,Al Menhali A

    更新日期:2019-08-05 00:00:00

  • RhoA/ROCK Regulates Prion Pathogenesis by Controlling Connexin 43 Activity.

    abstract::Scrapie infection, which converts cellular prion protein (PrPC) into the pathological and infectious isoform (PrPSc), leads to neuronal cell death, glial cell activation and PrPSc accumulation. Previous studies reported that PrPC regulates RhoA/Rho-associated kinase (ROCK) signaling and that connexin 43 (Cx43) express...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21041255

    authors: Kim HJ,Kim MJ,Mostafa MN,Park JH,Choi HS,Kim YS,Choi EK

    更新日期:2020-02-13 00:00:00

  • Molecular Signature of Indeterminate Thyroid Lesions: Current Methods to Improve Fine Needle Aspiration Cytology (FNAC) Diagnosis.

    abstract::Fine needle aspiration cytology (FNAC) represents the gold standard for determining the nature of thyroid nodules. It is a reliable method with good sensitivity and specificity. However, indeterminate lesions remain a diagnostic challenge and researchers have contributed molecular markers to search for in cytological ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18040775

    authors: Cantara S,Marzocchi C,Pilli T,Cardinale S,Forleo R,Castagna MG,Pacini F

    更新日期:2017-04-06 00:00:00

  • Analgesic Mechanisms of Antidepressants for Neuropathic Pain.

    abstract::Tricyclic antidepressants and serotonin noradrenaline reuptake inhibitors are used to treat chronic pain, such as neuropathic pain. Why antidepressants are effective for treatment of neuropathic pain and the precise mechanisms underlying their effects, however, remain unclear. The inhibitory effects of these antidepre...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18112483

    authors: Obata H

    更新日期:2017-11-21 00:00:00

  • Application of Chromatographic and Spectroscopic Methods towards the Quality Assessment of Ginger (Zingiber officinale) Rhizomes from Ecological Plantations.

    abstract::The usefulness of ginger in the food industry and pharmacotherapy is strictly related to its content of various components. The study elucidates the chemical composition of Zingiber officinale rhizomes cultivated on ecological plantations on Shikoku Island (Japan). GC-MS analysis of terpene content, LC-MS determinatio...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18020452

    authors: Koch W,Kukula-Koch W,Marzec Z,Kasperek E,Wyszogrodzka-Koma L,Szwerc W,Asakawa Y

    更新日期:2017-02-20 00:00:00

  • The Use of Stem Cell Differentiation Stage Factors (SCDSFs) Taken from Zebrafish Embryos during Organogenesis and Their Role in Regulating the Gene Expression of Normal and Pathological (Stem) Cells.

    abstract::Studies conducted on Zebrafish embryos in our laboratory have allowed for the identification of precise moments of organogenesis in which a lot of genes are switched on and off, a sign that the genome is undergoing substantial changes in gene expression. Stem cell growth and differentiation stage-factors present in di...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21144914

    authors: Biava PM

    更新日期:2020-07-12 00:00:00

  • Diverse Actions of Astrocytes in GABAergic Signaling.

    abstract::An imbalance of excitatory and inhibitory neurotransmission leading to over excitation plays a crucial role in generating seizures, while enhancing GABAergic mechanisms are critical in terminating seizures. In recent years, it has been reported in many studies that astrocytes are deeply involved in synaptic transmissi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20122964

    authors: Ishibashi M,Egawa K,Fukuda A

    更新日期:2019-06-18 00:00:00

  • Modulation of CAT-2B-Mediated l-Arginine Uptake and Nitric Oxide Biosynthesis in HCT116 Cell Line Through Biological Activity of 4'-Geranyloxyferulic Acid Extract from Quinoa Seeds.

    abstract::Chenopodium quinoa Wild is a "pseudocereal" grain which attracts a lot of attention in the scientific community as it has a positive effect on health. Here, we investigate the presence of biologically active O-prenylated phenylpropanoids in the ethanol extract of commercially available quinoa seeds. We claim that 4'-G...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20133262

    authors: Franceschelli S,Gatta DMP,Pesce M,Ferrone A,Quiles JL,Genovese S,Epifano F,Fiorito S,Taddeo VA,Patruno A,Grilli A,Felaco M,Speranza L

    更新日期:2019-07-02 00:00:00

  • Comparative Solid-State Stability of Perindopril Active Substance vs. Pharmaceutical Formulation.

    abstract::This paper presents the results obtained after studying the thermal stability and decomposition kinetics of perindopril erbumine as a pure active pharmaceutical ingredient as well as a solid pharmaceutical formulation containing the same active pharmaceutical ingredient (API). Since no data were found in the literatur...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18010164

    authors: Buda V,Andor M,Ledeti A,Ledeti I,Vlase G,Vlase T,Cristescu C,Voicu M,Suciu L,Tomescu MC

    更新日期:2017-01-15 00:00:00

  • Interaction between Mesenchymal Stem Cells and B-Cells.

    abstract::Mesenchymal stem cells (MSCs) are multipotent; non-hematopoietic stem cells. Because of their immunoregulatory abilities; MSCs are widely used for different clinical applications. Compared with that of other immune cells; the investigation of how MSCs specifically regulate B-cells has been superficial and insufficient...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms17050650

    authors: Fan L,Hu C,Chen J,Cen P,Wang J,Li L

    更新日期:2016-05-05 00:00:00

  • Epigenetic Targets for Oligonucleotide Therapies of Pulmonary Arterial Hypertension.

    abstract::Arterial wall remodeling underlies increased pulmonary vascular resistance and right heart failure in pulmonary arterial hypertension (PAH). None of the established vasodilator drug therapies for PAH prevents or reverse established arterial wall thickening, stiffening, and hypercontractility. Therefore, new approaches...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21239222

    authors: Gerthoffer W

    更新日期:2020-12-03 00:00:00

  • The Effect of Sodium Butyrate on Adventitious Shoot Formation Varies among the Plant Species and the Explant Types.

    abstract::Histone acetylation plays an important role in plant growth and development. Here, we investigated the effect of sodium butyrate (NaB), a histone deacetylase inhibitor, on adventitious shoot formation from protoplast-derived calli and cotyledon explants of tobacco (Nicotiana benthamiana) and tomato (Solanum lycopersic...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21228451

    authors: Lee MH,Lee J,Choi SH,Jie EY,Jeong JC,Kim CY,Kim SW

    更新日期:2020-11-10 00:00:00

  • Bevacizumab for Patients with Recurrent Multifocal Glioblastomas.

    abstract::In patients with glioblastoma, antiangiogenic therapy with bevacizumab (BEV) has been shown to improve progression-free survival (PFS), but not overall survival (OS). Especially in patients with an unusual infiltrative phenotype as seen in multifocal glioblastoma, the use of BEV therapy is still more controversial. Th...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18112469

    authors: Burger MC,Breuer S,Cieplik HC,Harter PN,Franz K,Bähr O,Steinbach JP

    更新日期:2017-11-20 00:00:00