Abstract：
:Basic fibroblast growth factor (bFGF) is a multifunctional factor in acceleration of cell proliferation, differentiation and transference, and therefore widely used in clinical applications. In this study, expression vector pWX-Nt03 harboring a codon-optimized bFGF gene was constructed and introduced into the tobacco chloroplasts by particle bombardment. After four rounds of selection, bFGF was proved to integrate into the chloroplast genome of regenerated plants and two of four transgenic plants were confirmed to be homoplastomic by PCR and Southern hybridization. ELISA assay indicated that bFGF represented approximately 0.1% of total soluble protein in the leaves of transplastomic tobacco plants. This is the first report of bFGF expression via chloroplast transformation in model plant, providing an additional option for the production of chloroplast-produced therapeutic proteins.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Wang YP,Wei ZY,Zhong XF,Lin CJ,Cai YH,Ma J,Zhang YY,Liu YZ,Xing SCdoi
10.3390/ijms17010019subject
Has Abstractpub_date
2015-12-23 00:00:00issue
1issn
1422-0067pii
ijms17010019journal_volume
17pub_type
杂志文章abstract：:Following the epidemics of obesity due to the consumption of high-calorie diet and sedentary lifestyle, nonalcoholic fatty liver disease (NAFLD) is now the leading cause of liver disease in Western countries. NAFLD is epidemiologically associated with metabolic syndrome and insulin resistance, and in susceptible indiv...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18071534
更新日期：2017-07-16 00:00:00
abstract：:Heterogeneous nuclear ribonucleoproteins (hnRNPs) are structurally and functionally distinct proteins containing specific domains and motifs that enable the proteins to bind certain nucleotide sequences, particularly those found in human telomeres. In human malignant cells (HMCs), hnRNP-A1-the most studied hnRNP-is an...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20030745
更新日期：2019-02-10 00:00:00
abstract：:Saturated aqueous solutions of various common salts were examined for their effect on aqueous aldol reactions catalysted by a highly active C(2)-symmetric diprolinamide organocatalyst developed in our laboratory. With respect to the aldol reaction between cyclohexanone and 4-nitrobenzaldehyde, deionised water was alwa...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12129083
更新日期：2011-01-01 00:00:00
abstract：:Antimicrobial peptides have been identified as one of the alternatives to the extensive use of common antibiotics as they show a broad spectrum of activity against human pathogens. Among these is Chionodracine (Cnd), a host-defense peptide isolated from the Antarctic icefish Chionodracohamatus, which belongs to the fa...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041401
更新日期：2020-02-19 00:00:00
abstract：:Glioblastoma (GBM) is the most common malignant brain tumor and its malignant phenotypic characteristics are classified as grade IV tumors. Molecular interactions, such as protein-protein, protein-ncRNA, and protein-peptide interactions are crucial to transfer the signaling communications in cellular signaling pathway...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020517
更新日期：2021-01-06 00:00:00
abstract：:Esophageal squamous cell carcinoma (ESCC), a major histologic type of esophageal cancer, is one of the frequent causes of cancer-related death worldwide. Picropodophyllotoxin (PPT) is the main component of Podophyllum hexandrum root with antitumor activity via apoptosis-mediated mechanisms in several cancer cells. How...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2020-06-30 00:00:00
abstract：:Apolipoprotein M (apoM) is a plasma apolipoprotein that mainly associates with high-density lipoproteins. Hence, most studies on apoM so far have investigated its effect on and association with lipid metabolism and atherosclerosis. The insight into apoM biology recently took a major turn. ApoM was identified as a carr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14034419
更新日期：2013-02-25 00:00:00
abstract：:Sequence diversity and population structures can vary widely among pathogenic bacteria species. In some species, all isolates are highly similar, whereas in others most of the isolates are distinguished easily. H. pylori is known for its wide genetic diversity amongst the various strains most especially in the genes i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12117459
更新日期：2011-01-01 00:00:00
abstract：:Parkinson's disease (PD) is the second most common neurodegenerative disease and although many studies have been done on this disease, the underlying mechanisms are still poorly understood and further studies are warranted. Therefore, this study identified circRNA expression profiles in the cerebral cortex (CC), hippo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2020-04-24 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2020-02-18 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2020-10-12 00:00:00
abstract：:A series of schizonepetin derivatives have been designed and synthesized in order to obtain potent antivirus agents. The antiviral activity against HSV-1 and influenza virus H3N2 as well as the cytotoxicity of these derivatives was evaluated by using cytopathic effect (CPE) inhibition assay in vitro. Compounds M2, M4,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140817193
更新日期：2013-08-20 00:00:00
abstract：:Perinatal stem cells have been regarded as an attractive and available cell source for medical research and clinical trials in recent years. Multiple stem cell types have been identified in the human placenta. Recent advances in knowledge on placental stem cells have revealed that human amniotic epithelial stem cells ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2020-10-19 00:00:00
abstract：:Oral biofilms attach onto both teeth surfaces and dental material surfaces in oral cavities. In the meantime, oral biofilms are not only the pathogenesis of dental caries and periodontitis, but also secondary caries and peri-implantitis, which would lead to the failure of clinical treatments. The material surfaces exp...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19103157
更新日期：2018-10-14 00:00:00
abstract：:Following major advances in the field of medicinal chemistry, novel drugs can now be designed systematically, instead of relying on old trial and error approaches. Current drug design strategies can be classified as being either ligand- or structure-based depending on the design process. In this paper, by describing t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2011-01-01 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19103251
更新日期：2018-10-19 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18030527
更新日期：2017-02-28 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21239082
更新日期：2020-11-29 00:00:00
abstract：:Intra-articular injection of platelet-rich plasma (PRP) has been established as a suitable treatment for knee osteoarthritis. Here, we present a double-blind randomized controlled clinical trial, conducted in a public Hospital of the Spanish National Health Care System, to evaluate the efficacy of injecting autologous...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,多中心研究,随机对照试验
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更新日期：2016-07-02 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2020-05-28 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2020-04-17 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2019-08-25 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2020-09-07 00:00:00
abstract：:This experiment aimed to investigate the effects of the chitosan (CTS) and water-soluble chitosan (WSC) microspheres on plasma lipids in male Sprague-Dawley rats fed with high-fat diets. CTS microspheres and WSC microspheres were prepared by the spray-drying technique. Scanning electron microscopy (SEM) micrographs s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14024174
更新日期：2013-02-19 00:00:00
abstract：:Atherosclerosis, as a chronic inflammatory disease, is the major underlying cause of death worldwide. However, the mechanisms that underlie the inflammatory process are not completely understood. Prolyl-isomerase-1 (Pin1), as a unique peptidyl-prolyl isomerase, plays an important role in inflammation and endothelial d...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18030644
更新日期：2017-03-16 00:00:00
abstract：:Gap junction (GJ) channels in invertebrates have been used to understand cell-to-cell communication in vertebrates. GJs are a common form of intercellular communication channels which connect the cytoplasm of adjacent cells. Dysregulation and structural alteration of the gap junction-mediated communication have been p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20102476
更新日期：2019-05-19 00:00:00
abstract：:G protein-coupled receptors are linked to various intracellular transducers, each pathway associated with different physiological effects. Biased ligands, capable of activating one pathway over another, are gaining attention for their therapeutic potential, as they could selectively activate beneficial pathways whilst...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249728
更新日期：2020-12-20 00:00:00
abstract：:The present study aimed to elucidate how Atlantic salmon adipocytes pre-enriched with palmitic (16:0, PA), oleic (18:1n-9, OA), or eicosapentaenoic (20:5n-3, EPA) acid respond to a fasting condition mimicked by nutrient deprivation and glucagon. All experimental groups were supplemented with radiolabeled PA to trace s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21072332
更新日期：2020-03-27 00:00:00
abstract：:The extracellular matrix (ECM) provides structural support for tissue architecture and is a major effector of cell behavior during skin repair and inflammation. Macrophages are involved in all stages of skin repair but only limited knowledge exists about macrophage-specific expression and regulation of ECM components....
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205086
更新日期：2019-10-14 00:00:00
abstract：:During plant-pathogen interactions, plants have to relocate their resources including energy to defend invading organisms; as a result, plant growth and development are usually reduced. Arabidopsis signal responsive1 (AtSR1) has been documented as a negative regulator of plant immune responses and could serve as a pos...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19061764
更新日期：2018-06-14 00:00:00
