Increase in Toxicity of Anticancer Drugs by PMTPV, a Claudin-1-Binding Peptide, Mediated via Down-Regulation of Claudin-1 in Human Lung Adenocarcinoma A549 Cells.

Abstract:

:Claudin-1 (CLDN1), a tight junctional protein, is highly expressed in lung cancer cells and may contribute to chemoresistance. A drug which decreases CLDN1 expression could be a chemosensitizer for enhancing the efficacy of anticancer drugs, but there is no such drug known. We found that PMTPV, a short peptide, which mimics the structure of second extracellular loop (ECL2) of CLDN1, can reduce the protein level of CLDN1 without affecting the mRNA level in A549 cells derived from human lung adenocarcinoma. The PMTPV-induced decrease in CLDN1 expression was inhibited by monodansylcadaverine, a clathrin-mediated endocytosis inhibitor, and chloroquine, a lysosome inhibitor. Quartz crystal microbalance assay showed that PMTPV can directly bind to the ECL2 of CLDN1. In transwell assay, PMTPV increased fluxes of Lucifer yellow (LY), a paracellular flux marker, and doxorubicin (DXR), an anthracycline anticancer drug, without affecting transepithelial electrical resistance. In three-dimensional spheroid culture, the size and cell viability were unchanged by short peptides, but the fluorescence intensity of hypoxia probe LOX-1 was decreased by PMTPV. PMTPV elevated the accumulation and cytotoxicity of DXR in the spheroids. Similar results were observed by knockdown of CLDN1. Furthermore, the sensitivities to cisplatin (CDDP), docetaxel, and gefitinib were enhanced by PMTPV. The level of CLDN1 expression in CDDP-resistant cells was higher than that in parental A549 cells, which was reduced by PMTPV. PMTPV restored the toxicity to DXR in the CDDP-resistant cells. Our data suggest that PMTPV may become a novel chemosensitizer for lung adenocarcinoma.

journal_name

Int J Mol Sci

authors

Nasako H,Takashina Y,Eguchi H,Ito A,Ishikawa Y,Matsunaga T,Endo S,Ikari A

doi

10.3390/ijms21165909

subject

Has Abstract

pub_date

2020-08-17 00:00:00

issue

16

issn

1422-0067

pii

ijms21165909

journal_volume

21

pub_type

杂志文章
  • Toxic Effects of the Major Components of Diesel Exhaust in Human Alveolar Basal Epithelial Cells (A549).

    abstract::We investigated the toxicity of benzo[a]pyrene (B[a]P), 1-nitropyrene (1-NP) and 3-nitrobenzanthrone (3-NBA) in A549 cells. Cells were treated for 4 h and 24 h with: B[a]P (0.1 and 1 μM), 1-NP (1 and 10 μM) and 3-NBA (0.5 and 5 μM). Bulky DNA adducts, lipid peroxidation, DNA and protein oxidation and mRNA expression o...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms17091393

    authors: Rossner P,Strapacova S,Stolcpartova J,Schmuczerova J,Milcova A,Neca J,Vlkova V,Brzicova T,Machala M,Topinka J

    更新日期:2016-08-26 00:00:00

  • Cancer Salivary Biomarkers for Tumours Distant to the Oral Cavity.

    abstract::The analysis of saliva as a diagnostic approach for systemic diseases was proposed just two decades ago, but recently great interest in the field has emerged because of its revolutionary potential as a liquid biopsy and its usefulness as a non-invasive sampling method. Multiple molecules isolated in saliva have been p...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms17091531

    authors: Rapado-González Ó,Majem B,Muinelo-Romay L,López-López R,Suarez-Cunqueiro MM

    更新日期:2016-09-12 00:00:00

  • Targeting the Adenosinergic Axis in Chronic Lymphocytic Leukemia: A Way to Disrupt the Tumor Niche?

    abstract::Targeting adenosine triphosphate (ATP) metabolism and adenosinergic signaling in cancer is gaining momentum, as increasing evidence is showing their relevance in tumor immunology and biology. Chronic lymphocytic leukemia (CLL) results from the expansion of a population of mature B cells that progressively occupies the...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19041167

    authors: Vaisitti T,Arruga F,Deaglio S

    更新日期:2018-04-12 00:00:00

  • Inhibition of oxidative stress by low-molecular-weight polysaccharides with various functional groups in skin fibroblasts.

    abstract::The aim of this study was to evaluate the in cellulo inhibition of hydrogen-peroxide-induced oxidative stress in skin fibroblasts using different low-molecular-weight polysaccharides (LMPS) prepared from agar (LMAG), chitosan (LMCH) and starch (LMST), which contain various different functional groups (i.e., sulfate, a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms141019399

    authors: Chen SK,Hsu CH,Tsai ML,Chen RH,Drummen GP

    更新日期:2013-09-25 00:00:00

  • FACS-Based Proteomics Enables Profiling of Proteins in Rare Cell Populations.

    abstract::Understanding disease pathology often does not require an overall proteomic analysis of clinical samples but rather the analysis of different, often rare, subpopulations of cells in a heterogeneous mixture of cell types. For the isolation of pre-specified cellular subtypes, fluorescence activated cell sorting (FACS) i...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21186557

    authors: Maes E,Cools N,Willems H,Baggerman G

    更新日期:2020-09-08 00:00:00

  • Study on Thermal Decomposition Behaviors of Terpolymers of Carbon Dioxide, Propylene Oxide, and Cyclohexene Oxide.

    abstract::The terpolymerization of carbon dioxide (CO₂), propylene oxide (PO), and cyclohexene oxide (CHO) were performed by both random polymerization and block polymerization to synthesize the random poly (propylene cyclohexene carbonate) (PPCHC), di-block polymers of poly (propylene carbonate⁻cyclohexyl carbonate) (PPC-PCHC)...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19123723

    authors: Chen S,Xiao M,Sun L,Meng Y

    更新日期:2018-11-23 00:00:00

  • Do Atypical Antipsychotics Have Antisuicidal Effects? A Hypothesis-Generating Overview.

    abstract::Modern antipsychotic drugs are employed increasingly in the treatment of mood disorders as well as psychoses, stimulating interest in their possible contributions to altering suicidal risk. Clozapine remains the only treatment with an FDA-recognized indication for reducing suicidal risk (in schizophrenia). We carried ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms17101700

    authors: Pompili M,Baldessarini RJ,Forte A,Erbuto D,Serafini G,Fiorillo A,Amore M,Girardi P

    更新日期:2016-10-11 00:00:00

  • Estrogen Abolishes the Repression Role of gga-miR-221-5p Targeting ELOVL6 and SQLE to Promote Lipid Synthesis in Chicken Liver.

    abstract::Few studies have been conducted regarding the biological function and regulation role of gga-miR-221-5p in the liver. We compared the conservation of miR-221-5p among species and investigated the expression pattern of gga-miR-221-5p, validating the direct target genes of gga-miR-221-5p by dual luciferase reporter assa...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21051624

    authors: Zhang DD,Wang DD,Wang Z,Wang YB,Li GX,Sun GR,Tian YD,Han RL,Li ZJ,Jiang RR,Liu XJ,Kang XT,Li H

    更新日期:2020-02-27 00:00:00

  • Modulatory Effects of Silymarin on Benzo[a]pyrene-Induced Hepatotoxicity.

    abstract::Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a group 1 carcinogen that introduces mutagenic DNA adducts into the genome. In this study, we investigated the molecular mechanisms underlying the involvement of silymarin in the reduction of DNA adduct formation by B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE)...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21072369

    authors: Jee SC,Kim M,Sung JS

    更新日期:2020-03-30 00:00:00

  • Picropodophyllotoxin, an Epimer of Podophyllotoxin, Causes Apoptosis of Human Esophageal Squamous Cell Carcinoma Cells Through ROS-Mediated JNK/P38 MAPK Pathways.

    abstract::Esophageal squamous cell carcinoma (ESCC), a major histologic type of esophageal cancer, is one of the frequent causes of cancer-related death worldwide. Picropodophyllotoxin (PPT) is the main component of Podophyllum hexandrum root with antitumor activity via apoptosis-mediated mechanisms in several cancer cells. How...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21134640

    authors: Kwak AW,Yoon G,Lee MH,Cho SS,Shim JH,Chae JI

    更新日期:2020-06-30 00:00:00

  • Application of WES Towards Molecular Investigation of Congenital Cataracts: Identification of Novel Alleles and Genes in a Hospital-Based Cohort of South India.

    abstract::Congenital cataracts are the prime cause for irreversible blindness in children. The global incidence of congenital cataract is 2.2-13.6 per 10,000 births, with the highest prevalence in Asia. Nearly half of the congenital cataracts are of familial nature, with a predominant autosomal dominant pattern of inheritance. ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21249569

    authors: Kandaswamy DK,Prakash MVS,Graw J,Koller S,Magyar I,Tiwari A,Berger W,Santhiya ST

    更新日期:2020-12-16 00:00:00

  • Astragalus membranaceus inhibits peritoneal fibrosis via monocyte chemoattractant protein (MCP)-1 and the transforming growth factor-β1 (TGF-β1) pathway in rats submitted to peritoneal dialysis.

    abstract::Inflammation and transforming growth factor-β1 (TGF-β1) contribute to the development of peritoneal fibrosis (PF), which is associated with peritoneal dialysis (PD). Astragalus membranaceus (Astragalus) has anti-inflammatory and anti-fibrotic effects in many diseases. The goal of this study was to determine the anti-f...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms150712959

    authors: Li Z,Zhang L,He W,Zhu C,Yang J,Sheng M

    更新日期:2014-07-22 00:00:00

  • Enzymatic Biotransformation of Balloon Flower Root Saponins into Bioactive Platycodin D by Deglucosylation with Caldicellulosiruptor bescii β-Glucosidase.

    abstract::Platycodin D (PD), a major saponin (platycoside) in Platycodi radix (balloon flower root), has higher pharmacological activity than the other major platycosides; however, its content in the plant root is only approximately 10% (w/w) and the productivities of PD by several enzymes are still too low for industrial appli...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20163854

    authors: Kil TG,Kang SH,Kim TH,Shin KC,Oh DK

    更新日期:2019-08-07 00:00:00

  • Applications of Alternative Nucleases in the Age of CRISPR/Cas9.

    abstract::Breakthroughs in the development of programmable site-specific nucleases, including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), meganucleases (MNs), and most recently, the clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (including Cas9)...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18122565

    authors: Guha TK,Edgell DR

    更新日期:2017-11-29 00:00:00

  • Evaluation of the antiradical properties of phenolic acids.

    abstract::Antioxidant capacity (AOC) against peroxyl radical and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical cation was measured for a series of p-hydroxybenzoic (HB) and p-hydroxycinnamic (HC) acids at different pH. Quantum-chemical computation was performed using Gaussian 3.0 softwa...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms150916351

    authors: Koroleva O,Torkova A,Nikolaev I,Khrameeva E,Fedorova T,Tsentalovich M,Amarowicz R

    更新日期:2014-09-16 00:00:00

  • Silibinin causes apoptosis and cell cycle arrest in some human pancreatic cancer cells.

    abstract::Silibinin, an effective anti-cancer and chemopreventive agent in various epithelial cancer models, has been reported to inhibit cancer cell growth through mitogenic signaling pathways. However, whether it can inhibit human pancreatic carcinoma growth and what are the underlying mechanisms is still not well elucidated....

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms12084861

    authors: Ge Y,Zhang Y,Chen Y,Li Q,Chen J,Dong Y,Shi W

    更新日期:2011-01-01 00:00:00

  • Colloidal Stability & Conformational Changes in β-Lactoglobulin: Unfolding to Self-Assembly.

    abstract::A detailed understanding of the mechanism of unfolding, aggregation, and associated rheological changes is developed in this study for β-Lactoglobulin at different pH values through concomitant measurements utilizing dynamic light scattering (DLS), optical microrheology, Raman spectroscopy, and differential scanning c...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms160817719

    authors: Blake S,Amin S,Qi W,Majumdar M,Lewis EN

    更新日期:2015-08-03 00:00:00

  • Degradation of microcystin-LR and RR by a Stenotrophomonas sp. strain EMS isolated from Lake Taihu, China.

    abstract::A bacterial strain EMS with the capability of degrading microcystins (MCs) was isolated from Lake Taihu, China. The bacterium was tentatively identified as a Stenotrophomonas sp. The bacterium could completely consume MC-LR and MC-RR within 24 hours at a concentration of 0.7 microg/mL and 1.7 microg/mL, respectively. ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms11030896

    authors: Chen J,Hu LB,Zhou W,Yan SH,Yang JD,Xue YF,Shi ZQ

    更新日期:2010-03-02 00:00:00

  • 3D radiation therapy boost improves the outcome of whole brain radiation therapy treated RPA II patients with one or two brain metastases.

    abstract:PURPOSE:to evaluate the role of whole brain radiotherapy (WBRT) and radiation boost (RB) for 208 patients recursive partitioning analysis (RPA) II with 1 or 2 brain metastases (BM) at a single institution. METHODS AND MATERIALS:the dose of WBRT was 30 Gy (10 fractions of 3 Gy). One hundred thirty-two patients (63.5%) ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms15057554

    authors: Antoni D,Clavier JB,Pop M,Schumacher C,Lefebvre F,Noël G

    更新日期:2014-05-02 00:00:00

  • The role of microRNAs in breast cancer migration, invasion and metastasis.

    abstract::MicroRNAs (miRNAs) are a major class of small, noncoding RNA molecules that regulate gene expression by targeting mRNAs to trigger either translational repression or mRNA degradation. They have recently been more widely investigated due to their potential role as targets for cancer therapy. Many miRNAs have been impli...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms131013414

    authors: Tang J,Ahmad A,Sarkar FH

    更新日期:2012-10-18 00:00:00

  • DNA Double Strand Break Response and Limited Repair Capacity in Mouse Elongated Spermatids.

    abstract::Spermatids are extremely sensitive to genotoxic exposures since during spermiogenesis only error-prone non homologous end joining (NHEJ) repair pathways are available. Hence, genomic damage may accumulate in sperm and be transmitted to the zygote. Indirect, delayed DNA fragmentation and lesions associated with apoptot...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms161226214

    authors: Ahmed EA,Scherthan H,de Rooij DG

    更新日期:2015-12-16 00:00:00

  • AKIRIN1: A Potential New Reference Gene in Human Natural Killer Cells and Granulocytes in Sepsis.

    abstract::Timely and reliable distinction of sepsis from non-infectious systemic inflammatory response syndrome (SIRS) supports adequate antimicrobial therapy and saves lives but is clinically challenging. Blood transcriptional profiling promises to deliver insights into the pathomechanisms of SIRS and sepsis and to accelerate ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20092290

    authors: Coulibaly A,Velásquez SY,Sticht C,Figueiredo AS,Himmelhan BS,Schulte J,Sturm T,Centner FS,Schöttler JJ,Thiel M,Lindner HA

    更新日期:2019-05-09 00:00:00

  • MiR-21 Protected Cardiomyocytes against Doxorubicin-Induced Apoptosis by Targeting BTG2.

    abstract::Doxorubicin (DOX) is an anthracycline drug with a wide spectrum of antineoplastic activities. However, it causes cardiac cytotoxicity, and this limits its clinical applications. MicroRNA-21 (miR-21) plays a vital role in regulating cell proliferation and apoptosis. While miR-21 is preferentially expressed in adult car...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms160714511

    authors: Tong Z,Jiang B,Wu Y,Liu Y,Li Y,Gao M,Jiang Y,Lv Q,Xiao X

    更新日期:2015-06-26 00:00:00

  • Characteristics and Antitumor Activity of Morchella esculenta Polysaccharide Extracted by Pulsed Electric Field.

    abstract::Polysaccharides from Morchella esculenta have been proven to be functional and helpful for humans. The purpose of this study was to investigate the chemical structure and anti-proliferating and antitumor activities of a Morchella esculenta polysaccharide (MEP) extracted by pulsed electric field (PEF) in submerged ferm...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms17060986

    authors: Liu C,Sun Y,Mao Q,Guo X,Li P,Liu Y,Xu N

    更新日期:2016-06-22 00:00:00

  • The yin and yang of VEGF and PEDF: multifaceted neurotrophic factors and their potential in the treatment of Parkinson's Disease.

    abstract::Over the last few decades, vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) have emerged as multifaceted players in not only the pathogenesis, but potential treatment, of numerous diseases. They activate diverse intracellular signaling cascades known to have extensive crosstalk, a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms11082875

    authors: Falk T,Gonzalez RT,Sherman SJ

    更新日期:2010-08-05 00:00:00

  • TNF-α gene knockout in triple negative breast cancer cell line induces apoptosis.

    abstract::Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine involved in the promotion and progression of cancer, including triple negative breast cancer cells. Thus, there is significant interest in understanding the molecular signaling pathways that connect TNF-α with the survival of tumor cells. In our experi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms14010411

    authors: Pileczki V,Braicu C,Gherman CD,Berindan-Neagoe I

    更新日期:2012-12-24 00:00:00

  • LncRNA Structural Characteristics in Epigenetic Regulation.

    abstract::The rapid development of new generation sequencing technology has deepened the understanding of genomes and functional products. RNA-sequencing studies in mammals show that approximately 85% of the DNA sequences have RNA products, for which the length greater than 200 nucleotides (nt) is called long non-coding RNAs (l...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18122659

    authors: Wang C,Wang L,Ding Y,Lu X,Zhang G,Yang J,Zheng H,Wang H,Jiang Y,Xu L

    更新日期:2017-12-08 00:00:00

  • Nonenzymatic Spontaneous Oxidative Transformation of 5,6-Dihydroxyindole.

    abstract::Melanin is an important phenolic skin pigment found throughout the animal kingdom. Tyrosine and its hydroxylated product dopa provide the starting material for melanin biosynthesis in all animals. Through a set of well-established reactions, they are converted to 5,6-dihydroxyindole (DHI) and DHI-2-carboxylic acid (DH...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21197321

    authors: Sugumaran M,Evans J,Ito S,Wakamatsu K

    更新日期:2020-10-03 00:00:00

  • Molecular Predictors of Complete Response Following Neoadjuvant Chemotherapy in Urothelial Carcinoma of the Bladder and Upper Tracts.

    abstract::Urothelial carcinoma of the bladder (UCB) and upper tracts (UTUC) is often regarded as one entity and is managed generally with similar principles. While neoadjuvant chemotherapy (NAC) followed by radical cystectomy (RC) is an established standard of care in UCB, strong evidence for a similar approach is lacking in UT...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20040793

    authors: Tse J,Ghandour R,Singla N,Lotan Y

    更新日期:2019-02-13 00:00:00

  • Spliceosome-Associated microRNAs Signify Breast Cancer Cells and Portray Potential Novel Nuclear Targets.

    abstract::MicroRNAs (miRNAs) act as negative regulators of gene expression in the cytoplasm. Previous studies have identified the presence of miRNAs in the nucleus. Here we study human breast cancer-derived cell-lines (MCF-7 and MDA-MB-231) and a non-tumorigenic cell-line (MCF-10A) and compare their miRNA sequences at the splic...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21218132

    authors: Mahlab-Aviv S,Zohar K,Cohen Y,Peretz AR,Eliyahu T,Linial M,Sperling R

    更新日期:2020-10-30 00:00:00