Abstract：
:Aeromonas veronii is a pathogen capable of infecting humans, livestock and aquatic animals, resulting in serious economic losses. In this study, two recombinant Lactobacillus casei expressing flagellin A (FlaA) of A. veronii, Lc-pPG-1-FlaA (surface-displayed) and Lc-pPG-2-FlaA (secretory) were constructed. The immune responses in fish administered with recombinant L. casei were evaluated. The two recombinant L. casei were orally administered to common carp, which stimulated high serum IgM and induced higher ACP, AKP, SOD and LYZ activity. Using qRT-PCR, the expression of IL-10, IL-8, IL-1β, TNF-α and IFN-γ in the tissue of fish immunized with recombinant L. casei was significantly (p < 0.05) upregulated, which indicated that recombinant L. casei could activate the innate immune system to trigger the cell immune response and inflammatory response. Furthermore, recombinant L. casei was able to survive the intestinal environment and colonize in intestine mucosal. The study showed that after being challenged by A. veronii, fish administered with Lc-pPG-1-FlaA (70%) and Lc-pPG-2-FlaA (50%) had higher survival rates compared to Lc-pPG and PBS, indicating that recombinant L. casei might prevent A. veronii infection by activating the immune system to trigger immune responses. We demonstrated that flagellin as an antigen of vaccine, is acceptable for preventing A. veronii infection in fish. The recombinant L. casei expressing FlaA may be a novel mucosal vaccine for treating and controlling A. veronii.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Tian JX,Kang YH,Chu GS,Liu HJ,Kong YD,Zhao LH,Kong YX,Shan XF,Wang GQdoi
10.3390/ijms21010033subject
Has Abstractpub_date
2019-12-19 00:00:00issue
1issn
1422-0067pii
ijms21010033journal_volume
21pub_type
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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doi：10.3390/ijms14023395
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journal_title：International journal of molecular sciences
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abstract：:The author wishes to make a change to the published paper [1].[...]. ...
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pub_type： 杂志文章,评审
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journal_title：International journal of molecular sciences
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更新日期：2015-03-10 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2018-02-27 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2017-02-20 00:00:00
abstract：:Botrytis cinerea is a pathogenic fungus that causes gray mold disease in a broad range of crops. The high intraspecific variability of B. cinerea makes control of this fungus very difficult. Here, we isolated a variant B05.10M strain from wild-type B05.10. The B05.10M strain showed serious defects in mycelial growth, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082912
更新日期：2020-04-21 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197351
更新日期：2020-10-05 00:00:00
abstract：:Recent studies have identified cyclic GMP-AMP synthase (cGAS) as an important target for treating autoimmune diseases, and several inhibitors of human cGAS (hcGAS) and their structures in complexation with hcGAS have been reported. However, the mechanisms via which these inhibitors interact with hcGAS are not complete...
journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18010017
更新日期：2016-12-22 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160818384
更新日期：2015-08-07 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19082363
更新日期：2018-08-10 00:00:00
abstract：:Previously, we demonstrated that the immediate administration of multitarget anxiolytic afobazole slows down the progression of neuronal damage in a 6-hydroxidodamine (6-OHDA) model of Parkinson's disease due to the activation of chaperone Sigma1R. The aim of the present study is to evaluate the therapeutic potential ...
journal_title：International journal of molecular sciences
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doi：10.3390/ijms21207620
更新日期：2020-10-15 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2014-12-23 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2010-08-05 00:00:00
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更新日期：2017-07-12 00:00:00
