Abstract:
:Dysregulation of platelet function can contribute to the disease progression in sepsis. The proteasome represents a critical and vital element of cellular protein metabolism in platelets and its proteolytic activity has been associated with platelet function. However, the role of the platelet proteasome as well as its response to infection under conditions of sepsis have not been studied so far. We measured platelet proteasome activity by fluorescent substrates, degradation of poly-ubiquitinated proteins and cleavage of the proteasome substrate Talin-1 in the presence of living E. coli strains and in platelets isolated from sepsis patients. Upregulation of the proteasome activator PA28 (PSME1) was assessed by quantitative real-time PCR in platelets from sepsis patients. We show that co-incubation of platelets with living E. coli (UTI89) results in increased degradation of poly-ubiquitinated proteins and cleavage of Talin-1 by the proteasome. Proteasome activity and cleavage of Talin-1 was significantly increased in α-hemolysin (HlyA)-positive E. coli strains. Supporting these findings, proteasome activity was also increased in platelets of patients with sepsis. Finally, the proteasome activator PA28 (PSME1) was upregulated in this group of patients. In this study we demonstrate for the first time that the proteasome in platelets is activated in the septic milieu.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Grundler Groterhorst K,Mannell H,Pircher J,Kraemer BFdoi
10.3390/ijms20235961subject
Has Abstractpub_date
2019-11-27 00:00:00issue
23issn
1422-0067pii
ijms20235961journal_volume
20pub_type
杂志文章abstract::3,3'-Diindolylmethane (DIM) is a bioactive compound found in Cruciferous vegetables that possesses health benefits such as antioxidant, anticancer, and anti-inflammatory effects. However, hydrophobicity and photolabile limit its pharmaceutical applications. This study aims to prepare and characterize DIM-encapsulated ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20163917
更新日期:2019-08-12 00:00:00
abstract::Akt acts as a pivotal regulator in the PI3K/Akt signaling pathway and represents a potential drug target for cancer therapy. To search for new inhibitors of Akt kinase, we performed a structure-based virtual screening using the DOCK 4.0 program and the X-ray crystal structure of human Akt kinase. From the virtual scre...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16023202
更新日期:2015-02-02 00:00:00
abstract::Osteoporosis represents the most common bone disease worldwide and results in a significantly increased fracture risk. Extrinsic and intrinsic factors implicated in the development of osteoporosis are also associated with delayed fracture healing and impaired bone regeneration. Based on a steadily increasing life expe...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20010083
更新日期:2018-12-26 00:00:00
abstract::As the worldwide application of nanomaterials in commercial products increases every year, various nanoparticles from industry might present possible risks to aquatic systems and human health. Presently, there are many unknowns about the toxic effects of nanomaterials, especially because the unique physicochemical pro...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21082864
更新日期:2020-04-20 00:00:00
abstract::Future long-duration space missions will involve travel outside of the Earth's magnetosphere protection and will result in astronauts being exposed to high energy and charge (HZE) ions and protons. Exposure to this type of radiation can result in damage to the central nervous system and deficits in numerous cognitive ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20020339
更新日期:2019-01-15 00:00:00
abstract:BACKGROUND:Epstein-Barr Virus (EBV) positive and microsatellite unstable (MSI-high) gastric cancer (GC) are molecular subgroups with distinctive molecular profiles. We explored the transcriptomic differences between EBV+ and MSI-high GCs, and the expression of current GC immunotherapy targets such as PD-1, PD-L1, CTLA4...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19072079
更新日期:2018-07-17 00:00:00
abstract::The concept of regenerating tissues, with properties and functions that mimic natural tissues, has attracted significant attention in recent years. [...]. ...
journal_title:International journal of molecular sciences
pub_type: 社论
doi:10.3390/ijms19123960
更新日期:2018-12-09 00:00:00
abstract::The neuronal ceroid lipofuscinoses (NCLs) collectively constitute one of the most common forms of inherited childhood-onset neurodegenerative disorders. They form a heterogeneous group of incurable lysosomal storage diseases that lead to blindness, motor deterioration, epilepsy, and dementia. Traditionally the NCL dis...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21218055
更新日期:2020-10-29 00:00:00
abstract::Monoclonal antibody G250 (mAbG250) recognizes a determinant on carbonic anhydrase IX (CAIX). CAIX is expressed by virtually all renal cell carcinomas of the clear cell type (ccRCC), but expression in normal tissues is restricted. The homogeneous CAIX expression in ccRCC and excellent targeting capability of mAbG250 in...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms140611402
更新日期:2013-05-29 00:00:00
abstract::Acute erythroleukemia (AEL) is a rare disease typically associated with a poor prognosis. The median survival ranges between 3-9 months from initial diagnosis. Hypomethylating agents (HMAs) have been shown to prolong survival in patients with myelodysplastic syndromes (MDS) and AML, but there is limited data of their ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,多中心研究
doi:10.3390/ijms18040837
更新日期:2017-04-14 00:00:00
abstract::Telomere length (TL) influences the development of lifestyle-related diseases, and neonatal TL may influence their prevalence. Various factors have been reported to affect neonatal TL. Although the fetus is exposed to multiple conditions in utero, the main factors affecting the shortening of neonatal TL are still not ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249688
更新日期:2020-12-18 00:00:00
abstract::Fatty acid metabolism is important in the regulation of breast cancer progression. Some of the proteins involved in fatty acid transport have been demonstrated to promote the proliferation, migration, and invasion in breast cancer cells. Solute carrier family 27 member 4 (SLC27A4) is a fatty acid transporter protein a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19113434
更新日期:2018-11-01 00:00:00
abstract::The importance of magnesium (Mg) for plant growth is well-documented. Silicon (Si)-mediated alleviation of mineral deficiencies has been also reported in a number of plant species; however, there is no report on the relevance of Si nutrition in plants grown in Mg-deficient condition. Therefore, in the present work, an...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20040969
更新日期:2019-02-22 00:00:00
abstract::KCND3 encodes the voltage-gated potassium ion channel subfamily D member 3, a six trans-membrane protein (Kv4.3), involved in the transient outward K+ current. KCND3 defect causes both cardiological and neurological syndromes. From a neurological perspective, Kv4.3 defect has been associated to SCA type 19/22, a compl...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21165802
更新日期:2020-08-13 00:00:00
abstract::MCP-1-induced protein (MCPIP, also known as Zc3h12a or Regnase-1), a newly identified suppressor of cytokine signaling, is expressed in endothelial cells (ECs). To investigate the role of endothelial MCPIP in vascular homeostasis and function, we deleted the MCPIP gene specifically in ECs using the Cre-LoxP system. EC...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20010172
更新日期:2019-01-05 00:00:00
abstract::NRF2 (Nuclear factor Erythroid 2-related Factor 2) signaling is impaired in Friedreich's Ataxia (FRDA), an autosomal recessive disease characterized by progressive nervous system damage and degeneration of nerve fibers in the spinal cord and peripheral nerves. The loss of frataxin in patients results in iron sulfur cl...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20205211
更新日期:2019-10-21 00:00:00
abstract::Carbonic anhydrase is a ubiquitous metalloenzyme that catalyzes the reversible interconversion of CO2/HCO3-. Equilibrium of these species is maintained by the action of carbonic anhydrase. Recent advances in magnetic resonance spectroscopy have allowed, for the first time, in vivo characterization of carbonic anhydras...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21072442
更新日期:2020-04-01 00:00:00
abstract::Histone deacetylases have been a target of therapy for organ fibrosis. Here, we report the protective effect of CG200745 (CG), a novel histone deacetylase inhibitor, on tubulointerstitial fibrosis in Col4a3-/- mice, a murine model of Alport syndrome. Morphological analyses revealed CG treatment markedly alleviated kid...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041473
更新日期:2020-02-21 00:00:00
abstract::Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. How these proteome changes relate to the loss of sperm function during cryopreservation remains unsolved. The present study aimed to clarify this issue evaluating differential changes in the proteome of fresh and frozen-thawed p...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20071791
更新日期:2019-04-11 00:00:00
abstract::The analgesic effect of venlafaxine (VLX), which is a selective serotonin and noradrenaline reuptake inhibitor (SNRI), has been observed on oxaliplatin-induced neuropathic pain in mice. Significant allodynia was shown after oxaliplatin treatment (6 mg/kg, i.p.); acetone and von Frey hair tests were used to assess cold...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20071652
更新日期:2019-04-03 00:00:00
abstract::In 1993, Rosen and collaborators discovered that the gene encoding SOD1 has mutations in amyotrophic lateral sclerosis (ALS) patients; moreover, these mutations are found in the exon regions, suggesting that their toxic effects are the consequence of protein dysfunction with an increase of oxidative stress. While a cl...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19051345
更新日期:2018-05-03 00:00:00
abstract::Matrix metalloproteinases (MMPs) are enzymes involved in periodontal tissue destruction. Hemagglutinin B (HagB) from the periodontal pathogen Porphyromonas gingivalis induces an elevated MMP response in dendritic cells, but responses from cultures of single-cell types do not reflect the local tissue environment. The o...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19123923
更新日期:2018-12-07 00:00:00
abstract::The astA gene encoding an alternative sulfate transporter was originally cloned from the genome of the Japanese Aspergillus nidulans isolate as a suppressor of sulfate permease-deficient strains. Expression of the astA gene is under the control of the sulfur metabolite repression system. The encoded protein transports...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207727
更新日期:2020-10-19 00:00:00
abstract::Wilms tumor protein-1 (WT1) is an attractive target for adoptive T-cell therapy due to its expression in solid tumors and hematologic malignancies. However, T cells recognizing WT1 occur in low frequencies in the peripheral blood of healthy donors, limiting potential therapeutic possibilities. Tin mesoporphyrin (SnMP)...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20030482
更新日期:2019-01-23 00:00:00
abstract::Safflower (Carthamus tinctorius L.), an important traditional Chinese medicine, is cultured widely for its pharmacological effects, but little is known regarding the genes related to the metabolic regulation of the safflower's yellow pigment. To investigate genes related to safflor yellow biosynthesis, 454 pyrosequenc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms161025657
更新日期:2015-10-26 00:00:00
abstract::Legume root nodules develop as a result of a symbiotic relationship between the plant and nitrogen-fixing rhizobia bacteria in soil. Auxin activity is detected in different cell types at different stages of nodule development; as well as an enhanced sensitivity to auxin inhibits, which could affect nodule development....
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18122547
更新日期:2017-11-28 00:00:00
abstract::Updated measurements of charged particle fluxes during the transit from Earth to Mars as well as on site measurements by Curiosity of Martian surface radiation fluxes identified potential health hazards associated with radiation exposure for human space missions. Designing mitigation strategies of radiation risks to a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20010176
更新日期:2019-01-05 00:00:00
abstract::The effect of palmitoylethanolamide (PEA), an endogenous fatty acid amide displaying neuroprotective actions, on glutamate release from rat cerebrocortical nerve terminals (synaptosomes) was investigated. PEA inhibited the Ca²⁺-dependent release of glutamate, which was triggered by exposing synaptosomes to the potassi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16035555
更新日期:2015-03-11 00:00:00
abstract::Jatropha curcas is a promising renewable feedstock for biodiesel and bio-jet fuel production. To study gene expression in Jatropha in different tissues throughout development and under stress conditions, we examined a total of 11 typical candidate reference genes using real-time quantitative polymerase chain reaction ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms141224338
更新日期:2013-12-13 00:00:00
abstract::LEAFY (LFY) and UNUSUAL FLORAL ORGANS (UFO) homologous genes have been reported to play key roles in promoting the initiation of floral meristems in raceme- and cyme-type plants. Asteraceae, a large family of plants with more than 23,000 species, has a unique head-like inflorescence termed capitulum. Here, we report a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20102503
更新日期:2019-05-21 00:00:00