Low molecular weight heparin suppresses lymphatic endothelial cell proliferation induced by vascular endothelial growth factor C in vitro.

Abstract:

BACKGROUND:Pancreatic cancer is one of the most aggressive human malignancies. Lymphangiogenesis plays an important role in lymph node metastasis of many solid tumors. It is well known that low molecular weight heparins (LMWHs) can inhibit cell growth, cell invasion and angiogenesis, which are key processes in tumor progression. METHODS:We measured the expression of vascular endothelial growth factor C (VEGF-C) in pancreatic cancer cells (PANC-1) using reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. We used an in vitro assay to evaluate the anti-lymphangiogenic effect of an LMWH, Fragmin, on human lymphatic endothelial cell (HLEC) proliferation. RESULTS:Fragmin at a low concentration can effectively inhibits HLEC proliferation induced by VEGF-C. VEGF-C secreted by PANC-1 cells stimulated HLEC proliferation. Low concentration LMWH suppressed HLEC proliferation induced by VEGF-C but did not affect proliferation or VEGF-C expression of PANC-1 cells, whereas high concentrations of LMWH inhibited PANC-1 cell proliferation. CONCLUSIONS:These results suggest that VEGF-C released by cancer cells plays an important role in promoting HLEC proliferation. The LMWH Fragmin has anti-lymphangiogenic effects and may inhibit lymphatic metastasis in pancreatic cancer.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Cao G,Wu JX,Wu QH

subject

Has Abstract

pub_date

2009-07-05 00:00:00

pages

1570-4

issue

13

eissn

0366-6999

issn

2542-5641

journal_volume

122

pub_type

杂志文章
  • Clinical application and histological observation of pedicled buccal fat pad grafting.

    abstract:OBJECTIVE:To introduce the applied anatomy of pedicled buccal fat pad (BFP) graft and a series of histological changes in the healing process of BFP as an uncovered pedicled graft. METHODS:The healing processes of BFP are demonstrated via uncovered pedicled grafts on rabbits in histological examination. Uncovered bucc...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Fan L,Chen G,Zhao S,Hu J

    更新日期:2002-10-01 00:00:00

  • Hypothermia ameliorates gastrointestinal ischemic injury sustained in a porcine cardiac arrest model.

    abstract:BACKGROUND:During cardiac arrest, the gastrointestinal tract is sensitive to ischemia. Protection of the gastrointestinal tract is a critical factor in determining prognosis following cardiopulmonary resuscitation (CPR). This study seeks to determine the extent of gastrointestinal tract injury and the potential protect...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Lu Y,Wang S,Li CS

    更新日期:2012-12-01 00:00:00

  • Presence of serum antinuclear antibodies correlating unfavorable overall survival in patients with chronic lymphocytic leukemia.

    abstract:BACKGROUND:Serum antinuclear antibodies (ANAs) are positive in some patients with chronic lymphocytic leukemia (CLL), but the prognostic value of ANAs remains unknown. The aim of this study was to evaluate the role of ANAs as a prognostic factor in CLL. METHODS:This study retrospectively analyzed clinical data from 21...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.1097/CM9.0000000000000114

    authors: Sun Q,Wang L,Zhu HY,Miao Y,Wu W,Liang JH,Cao L,Xia Y,Wu JZ,Wang Y,Wang R,Fan L,Xu W,Li JY

    更新日期:2019-03-05 00:00:00

  • The utilization status of aspirin for the secondary prevention of ischemic stroke.

    abstract:BACKGROUND:The present study was aimed to investigate the usage of aspirin for the secondary prevention of ischemic stroke, evaluate the correlated factors, and analyze the reasons for not taking and irregularly taking aspirin. METHODS:The patients in this group were all stroke survivors who have formerly been diagnos...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Ke XJ,Yu YF,Guo ZL,Xu K,Hai H,Zhang AH,Jiang H,Peng H

    更新日期:2009-01-20 00:00:00

  • Glutathione S-transferase M1 and T1 genotypes and endometriosis risk: a case-controlled study.

    abstract:OBJECTIVE:To investigate the correlation between glutathione S-transferase (GST) M1 and T1 genotypes and endometriosis risk (EM). METHODS:Polymerase chain reaction (PCR) technique was used to detect the presence or absence of the GSTM1 and GSTT1 genes in genomic DNA isolated from the blood samples of 68 Han Chinese wo...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Lin J,Zhang X,Qian Y,Ye Y,Shi Y,Xu K,Xu J

    更新日期:2003-05-01 00:00:00

  • Electromyographic monitoring of facial nerve under different levels of neuromuscular blockade during middle ear microsurgery.

    abstract:BACKGROUND:The evoked electromyography (EMG) is frequently used to identify facial nerve in order to prevent its damage during surgeries. Partial neuromuscular blockade (NMB) has been suggested to favor EMG activity and insure patients' safety. The aim of this study was to determine an adequate level of NMB corresponde...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Cai YR,Xu J,Chen LH,Chi FL

    更新日期:2009-02-05 00:00:00

  • Involvement of nitric oxide in the vasodilator and depressor effect of calcitonin gene-related peptide.

    abstract::In present study, we examined the effects of NG-nitro-L-arginine (LNNA), an inhibitor of nitric oxide synthase (NOS), and/or methylene blue (MB), a blocker of guanylate cyclase on the vasodilator response of isolated rat arteries including aorta and mesenteric artery to calcitonin gene-related peptide (CGRP) by in vit...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Tan DY,Zhang LZ,Zhao YT,Zhao D,Tang J

    更新日期:1994-10-01 00:00:00

  • Outcome and risk factors of early onset severe preeclampsia.

    abstract:BACKGROUND:Early onset severe preeclampsia is a specific type of severe preeclampsia, which causes high morbidity and mortality of both mothers and fetus. This study aimed to investigate the clinical definition, features, treatment, outcome and risk factors of early onset severe preeclampsia in Chinese women. METHODS:...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Gong YH,Jia J,Lü DH,Dai L,Bai Y,Zhou R

    更新日期:2012-07-01 00:00:00

  • Effect of respiratory syncytial virus on the activity of matrix metalloproteinase in mice.

    abstract:BACKGROUND:Respiratory syncytial virus (RSV) is a common pathogen in the lower respiratory tract of infants and children. Recent studies have shown that in adults, especially in the elderly population who have relatively weak immunity, lower respiratory tract infection is not uncommon. RSV was detected in 22% hospitali...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Li W,Shen HH

    更新日期:2007-01-05 00:00:00

  • Pancreatic and duodenal homeobox-1 in pancreatic ductal adenocarcinoma and diabetes mellitus.

    abstract::Diabetes mellitus and pancreatic ductal adenocarcinoma are two common diseases worldwidely which are both derived from different components of pancreas. The pancreatic and duodenal homeobox-1 (PDX1) is an essential transcription factor for the early development of pancreas that is required for the differentiation of a...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,评审

    doi:10.1097/CM9.0000000000000628

    authors: Tang ZC,Chu Y,Tan YY,Li J,Gao S

    更新日期:2020-02-05 00:00:00

  • Distribution and differentiation of mesenchymal stem cells in tumor tissue.

    abstract:BACKGROUND:Tumor has an ability to become enriched in mesenchymal stem cells (MSCs) and of guiding MSCs to migrate to tumor tissue. But there are lack of relevant reports on the distribution and differentiation of MSCs in tumor tissue and the effect on tumor growth after MSCs engrafted in tumor tissue. In this study, w...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Zhao HF,Chen J,Xu ZS,Zhang KQ

    更新日期:2009-03-20 00:00:00

  • Induced pluripotent stem cells.

    abstract::Induced pluripotent stem (iPS) cells are a recent development which has brought a promise of great therapeutic values. The previous technique of somatic cell nuclear transfer (SCNT) has been ineffective in humans. Recent discoveries show that human fibroblasts can be reprogrammed by a transient over expression of a sm...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Bhowmik S,Li Y

    更新日期:2011-06-01 00:00:00

  • Effects of mixture of lidocaine and ropivacaine at different concentrations on the central nervous system and cardiovascular toxicity in rats.

    abstract:BACKGROUND:Lidocaine and ropivacaine are often combined in clinical practice to obtain a rapid onset and a prolonged duration of action. However, the systemic toxicity of their mixture at different concentrations is unclear. This study aimed to compare the systemic toxicity of the mixture of ropivacaine and lidocaine a...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wan QX,Bo YL,Li HB,Li WZ

    更新日期:2010-01-05 00:00:00

  • Pathologic changes, mechanisms and diagnosis in renal bone disease.

    abstract:OBJECTIVE:To investigate the incidence rate, pathologic changes, mechanisms and diagnostic methods in renal bone disease. METHODS:The blood levels of carboxyterminal parpthyriod hormone (C-PTH), 1,25(OH)2D3, calcium and phosphate, aluminum in serum and bone tissue were measured. The bone biopsy and bone scan with 99m ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Zhu P,Wang G,Yu Y,Lou D,Wang S,Jia J,Dong D

    更新日期:1998-09-01 00:00:00

  • Management of intra-articular fracture of the fingers via mini external fixator combined with limited internal fixation.

    abstract:BACKGROUND:Intra-articular fractures of the fingers are common problems to emergency physicians and hand surgeons. Inappropriate management of these injuries may result in chronic pain, stiffness, deformity, or post traumatic arthritis. Ideal treatment necessitates the restoration of a stable and congruent joint that w...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Li WJ,Tian W,Tian GL,Chen SL,Zhang CQ,Xue YH,Li ZZ,Zhu Y

    更新日期:2009-11-05 00:00:00

  • Effect of Tbx1 knock-down on cardiac performance in zebrafish.

    abstract:BACKGROUND:Tbx1 is the major candidate gene for DiGeorge syndrome (DGS). Similar to defects observed in DGS patients, the structures disrupted in Tbx1(-/-) animal models are derived from the neural crest cells during development. Although the morphological phenotypes of some Tbx1 knock-down animal models have been well...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Zhang LF,Gui YH,Wang YX,Jiang Q,Song HY

    更新日期:2010-05-05 00:00:00

  • Screening for amblyopia among grade-1 students in primary school with uncorrected vision and stereopsis test in central China.

    abstract:BACKGROUND:Screening for amblyopia at earliest is important for early treatment and better prognosis. This study aimed to evaluate the validity of uncorrected distant and near visual acuity (VA) and stereoacuity for screening amblyopia in grade-1 students in primary school in central China. METHODS:By stratified clust...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Fu J,Li SM,Li JL,Li SY,Liu LR,Wang Y,Li H,Zhu BD,Ji YZ,Yang Z,Li L,Chen W,Wang NL,g Childhood Eye Study Group.

    更新日期:2013-03-01 00:00:00

  • In vitro study of safety and co-efficiency of the transbronchial coagulation techniques.

    abstract:BACKGROUND:The usual transbronchial coagulation techniques include microwave, argon plasma coagulation (APC), electrocautery and cryotherapy. However, there are serious clinical problems in the safety of each. By analyzing the experimental data and clinical observations, we observed the variable effects of different co...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Bai C,Dong YC,Song XL,Huang Y,Shi H,Hu ZL,Li Q

    更新日期:2013-01-01 00:00:00

  • Surgical treatment for vascular anomalies and tracheoesophageal compression.

    abstract:BACKGROUND:Vascular rings are uncommon anomalies in which preferred strategies for diagnosis and management may vary among institutions. In this study, we reported our approach and a review of our 5-year experience. METHODS:From May 2006 to April 2011, 45 children (31 boys) with vascular rings underwent surgical repai...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Bai S,Li XF,Liu CX,Peng Y,Yuan F,Guo J,Song ZJ,Novick WM,Li ZZ

    更新日期:2012-04-01 00:00:00

  • The role of CTLA4-Ig in a mouse model against allergic asthma.

    abstract:OBJECTIVE:To investigate CTLA4-Ig's potential role in therapy for allergic asthma by blocking B7/CD28 interactions with cytotoxic T lymphocyte antigen 4-Ig (CTLA4-Ig). METHODS:We divided BALB/C mice into the three groups: Sham/Sham (control), ovalbumin (OVA)/OVA and mCTLA4-Ig. Blood, bronchoalveolar lavage, histology ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wan H,Zhou M,Xu Q,Huang S,Deng W

    更新日期:2003-03-01 00:00:00

  • IMP3 is a novel biomarker to predict metastasis and prognosis of gastric adenocarcinoma: a retrospective study.

    abstract:BACKGROUND:Insulin-like growth factor-II mRNA-binding protein 3 (IMP3) is a newly identified mRNA-binding protein that is involved in embryogenesis and carcinogenesis of some malignant tumors. The aim of this study was to detect the expression of IMP3 protein in gastric adenocarcinoma (GAC) and the correlation with cli...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wang L,Li HG,Xia ZS,Lü J,Peng TS

    更新日期:2010-12-01 00:00:00

  • Influence of developmental cervical stenosis on dural sac space.

    abstract:BACKGROUND:This retrospective study aimed to investigate the difference of the intra-dural reserving space for spinal cord in magnetic resonance imaging (MRI) between patients with and without developmental cervical stenosis and its clinical significance. METHODS:A total of 264 patients with cervical spondylotic myelo...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Tang Y,Yu M,Liu Z,Sun Y,Liu X

    更新日期:2014-01-01 00:00:00

  • Biomechanical comparisons between a new avascular necrosis of femaral head stem based on Chinese patients with avascular necrosis and two other designs.

    abstract:BACKGROUND:There is a relatively high failure rate of the femoral component in patients with avascular necrosis at the intermediate-term follow-up. Improving the geometrical fit of the femoral stem against the medullary canal may help to provide long-term survivorship of the hip replacement for patients with avascular ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Zhang Q,Cheng CK,Wei HW,Dong X,Chen YT,Lai YS,Wang Y

    更新日期:2013-01-01 00:00:00

  • Pericardial devascularization combined with Latarjet's innervation in the treatment of patients with portal hypertension.

    abstract:OBJECTIVE:To study the effect of pericardial devascularization combined with Latarjet's innervation on portal hypertension. METHODS:Forty-eight patients undergoing pericardial devascularization combined with Latarjet's innervation were compared with 57 patients with devascularization. Clinical results were evaluated b...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Li S,Wu E,Huang J,Luo C

    更新日期:1998-12-01 00:00:00

  • An epidemic of encephalitis and meningoencephalitis in children caused by echovirus type 30 in Shanghai.

    abstract::An outbreak of viral central nervous system infection occurred from May to September, 1991 in Shanghai, China. Totally 376 cases were admitted into our hospital, 54% with encephalitis and 46% with meningoencephalitis. Echovirus type 30 was isolated from CSF and stools of the patients. Only 3 of the patients were under...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wang DM,Zhao GC,Zhuang SM,Zhang YC

    更新日期:1993-10-01 00:00:00

  • Influence of Medial Support Screws on the Maintenance of Fracture Reduction after Locked Plating of Proximal Humerus Fractures.

    abstract:Background:Technical aspects of the correct placement of medial support locking screws in the locking plate for proximal humerus fractures remain incompletely understood. This study was to evaluate the clinical relationship between the number of medial support screws and the maintenance of fracture reduction after lock...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.237396

    authors: Zeng LQ,Zeng LL,Jiang YW,Wei HF,Zhang W,Chen YF

    更新日期:2018-08-05 00:00:00

  • Emergency off-pump coronary artery surgery.

    abstract:BACKGROUND:Off-pump coronary artery bypass grafting is fast-becoming a procedure of choice for elective revascularization in high-risk patients with multi-vessel coronary artery disease. However, the role of off-pump coronary artery bypass grafting for patients with acute coronary syndromes requiring emergency revascul...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Raja SG,Haider Z,Zaman H

    更新日期:2004-06-01 00:00:00

  • Changes of T cell subsets in peripheral blood of patients with Schistosomiasis japonica and their relation to interleukin-1.

    abstract::T cell subsets in peripheral blood were phenotyped in 56 patients with different stages of Schistosomiasis japonica, including 17 with acute, 14 with chronic and 25 with advanced infection. The activity of interleukin-1 (IL-1) produced by peripheral blood mononuclear cells (PBMC) induced by lipopolysaccharide (LPS) in...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Li ZJ,Yang Q,Yang YX,Zeng LL,Li SL,Dai JZ,Wang HC,Luo DD,Wang XH

    更新日期:1993-03-01 00:00:00

  • Blood Pressure Targets in the Hypertensive Elderly.

    abstract:OBJECTIVE:Hypertension is an important risk factor of cardiovascular disease and increases mortality in the elderly. However, the available medical evidences are both inconsistent and insufficient regarding establishing credible and useful blood pressure (BP) targets in the hypertensive elderly. This review summarizes ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,评审

    doi:10.4103/0366-6999.211885

    authors: Liu P,Zheng JG

    更新日期:2017-08-20 00:00:00

  • New insight into genes in association with asthma: literature-based mining and network centrality analysis.

    abstract:BACKGROUND:Asthma is a heterogeneous disease for which a strong genetic basis has been firmly established. Until now no studies have been undertaken to systemically explore the network of asthma-related genes using an internally developed literature-based discovery approach. This study was to explore asthma-related gen...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Liang R,Wang L,Wang G

    更新日期:2013-07-01 00:00:00