Abstract:
BACKGROUND:Tbx1 is the major candidate gene for DiGeorge syndrome (DGS). Similar to defects observed in DGS patients, the structures disrupted in Tbx1(-/-) animal models are derived from the neural crest cells during development. Although the morphological phenotypes of some Tbx1 knock-down animal models have been well described, analysis of the cardiac performance is limited. Therefore, myocardial performance was explored in Tbx1 morpholino injected zebrafish embryos. METHODS:To elucidate these issues, Tbx1 specific morpholino was used to reduce the function of Tbx1 in zebrafish. The differentiation of the myocardial cells was observed using whole mount in situ hybridization. Heart rates were observed and recorded under the microscope from 24 to 72 hours post fertilization (hpf). The cardiac performance was analyzed by measuring ventricular shortening fraction and atrial shortening fraction. RESULTS:Tbx1 morpholino injected embryos were characterized by defects in the pharyngeal arches, otic vesicle, aortic arches and thymus. In addition, Tbx1 knock down reduced the amount of pharyngeal neural crest cells in zebrafish. Abnormal cardiac morphology was visible in nearly 20% of the Tbx1 morpholino injected embryos. The hearts in these embryos did not loop or loop incompletely. Importantly, cardiac performance and heart rate were reduced in Tbx1 morpholino injected embryos. CONCLUSIONS:Tbx1 might play an essential role in the development of pharyngeal neural crest cells in zebrafish. Cardiac performance is impaired by Tbx1 knock down in zebrafish.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Zhang LF,Gui YH,Wang YX,Jiang Q,Song HYsubject
Has Abstractpub_date
2010-05-05 00:00:00pages
1182-9issue
9eissn
0366-6999issn
2542-5641journal_volume
123pub_type
杂志文章abstract:BACKGROUND:In vivo quantification of choroidal neovascularization (CNV) based on noninvasive optical coherence tomography (OCT) examination and in vitro choroidal flatmount immunohistochemistry stained of CNV currently were used to evaluate the process and severity of age-related macular degeneration (AMD) both in huma...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.157681
更新日期:2015-06-05 00:00:00
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journal_title:Chinese medical journal
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journal_title:Chinese medical journal
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:
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pub_type: 杂志文章,meta分析
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pub_type: 杂志文章
doi:
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doi:
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doi:10.4103/0366-6999.151654
更新日期:2015-03-05 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-11-05 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2009-12-20 00:00:00
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pub_type: 杂志文章
doi:
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pub_type: 杂志文章
doi:
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doi:
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更新日期:2009-07-05 00:00:00
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pub_type: 杂志文章,多中心研究,随机对照试验
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doi:
更新日期:2012-04-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Chinese medical journal
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