Abstract:
:Most forms of tissue healing depend critically on revascularisation. In soft tissues and in vitro, mechanical stimuli have been shown to promote vessel-forming activity. However, in bone defects, increased interfragmentary motion impairs vascular regeneration. Because these effects seem contradictory, we aimed to determine whether a range of mechanical stimuli exists in which angiogenesis is favoured. A series of cyclic strain magnitudes were applied to a Matrigel-based "tube formation" assay and the total lengths of networks formed by human microvascular endothelial cells measured at 24 h. Network lengths were reduced at all strain levels, compared to unstretched controls. However, the levels of pro-angiogenic matrix metalloproteases-2 and -9 in the corresponding conditioned media were unchanged by strain, and vascular endothelial growth factor was uniformly elevated in stretched conditions. By repeating the assay with the addition of conditioned media from mesenchymal stem cells cultivated in similar conditions, paracrine stimuli were shown to increase network lengths, but not to alter the negative effect of cyclic stretching. Together, these results demonstrate that directly applied periodic strains can inhibit endothelial organisation in vitro, and suggest that this may be due to physical disruption rather than biochemical modulation. Most importantly, the results indicate that the straining of endothelial cells and their assembly into vascular-like structures must be studied simultaneously to adequately characterise the mechanical influence on vessel formation.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Wilson CJ,Kasper G,Schütz MA,Duda GNdoi
10.1016/j.mvr.2009.08.002subject
Has Abstractpub_date
2009-12-01 00:00:00pages
358-63issue
3eissn
0026-2862issn
1095-9319pii
S0026-2862(09)00226-Xjournal_volume
78pub_type
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journal_title:Microvascular research
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journal_title:Microvascular research
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journal_title:Microvascular research
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journal_title:Microvascular research
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journal_title:Microvascular research
pub_type: 杂志文章
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2008.08.001
更新日期:2009-03-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2009.12.004
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1995.1025
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journal_title:Microvascular research
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doi:10.1016/j.mvr.2009.12.001
更新日期:2010-03-01 00:00:00
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journal_title:Microvascular research
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