Abstract：
:Centric regions of eukaryotic genomes are packaged into heterochromatin, which possesses the ability to spread along the chromosome and silence gene expression. The process of spreading has been challenging to study at the molecular level due to repetitious sequences within centric regions. A heterochromatin protein 1 (HP1) tethering system was developed that generates "ectopic heterochromatin" at sites within euchromatic regions of the Drosophila melanogaster genome. Using this system, we show that HP1 dimerization and the PxVxL interaction platform formed by dimerization of the HP1 chromo shadow domain are necessary for spreading to a downstream reporter gene located 3.7 kb away. Surprisingly, either the HP1 chromo domain or the chromo shadow domain alone is sufficient for spreading and silencing at a downstream reporter gene located 1.9 kb away. Spreading is dependent on at least two H3K9 methyltransferases, with SU(VAR)3-9 playing a greater role at the 3.7-kb reporter and dSETDB1 predominately acting at the 1.9 kb reporter. These data support a model whereby HP1 takes part in multiple mechanisms of silencing and spreading.
journal_name
Geneticsjournal_title
Geneticsauthors
Hines KA,Cryderman DE,Flannery KM,Yang H,Vitalini MW,Hazelrigg T,Mizzen CA,Wallrath LLdoi
10.1534/genetics.109.105338subject
Has Abstractpub_date
2009-08-01 00:00:00pages
967-77issue
4eissn
0016-6731issn
1943-2631pii
genetics.109.105338journal_volume
182pub_type
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