Molecular recognition and self-assembly special feature: A general protocol for creating high-throughput screening assays for reaction yield and enantiomeric excess applied to hydrobenzoin.

abstract：:We have isolated and characterized the complete human villin gene. The villin gene is located on chromosome 2q35-36 in humans and on chromosome 1 in mice. Villin belongs to a family of calcium-regulated actin-binding proteins that share structural and functional homologies. The villin gene is expressed mainly in cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pringault E,Robine S,Louvard D

更新日期：1991-12-01 00:00:00

abstract：:We use quantized molecular dynamics simulations to characterize the role of enzyme vibrations in facilitating dihydrofolate reductase hydride transfer. By sampling the full ensemble of reactive trajectories, we are able to quantify and distinguish between statistical and dynamical correlations in the enzyme motion. We...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boekelheide N,Salomón-Ferrer R,Miller TF 3rd

更新日期：2011-09-27 00:00:00

abstract：:The neuraminidase inhibitors oseltamivir and zanamivi are used to treat H5N1 influenza. However, oseltamivir-resistant H5N1 viruses have been isolated from oseltamivir-treated patients. Moreover, reassortment between H5N1 viruses and oseltamvir-resistant human H1N1 viruses currently circulating could create oseltamivi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kiso M,Takahashi K,Sakai-Tagawa Y,Shinya K,Sakabe S,Le QM,Ozawa M,Furuta Y,Kawaoka Y

更新日期：2010-01-12 00:00:00

abstract：:Plasma membrane insulin receptors, affinity labeled by covalent crosslinking to receptor-bound 125I-labeled insulin, are shown to appear as a heterogeneous population of three major disulfide-linked complexes (Mr 350,000, 320,000, and 290,000) upon electrophoresis in highly porous dodecyl sulfate/polyacrylamide gels i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Massague J,Pilch PF,Czech MP

更新日期：1980-12-01 00:00:00

abstract：:The microvillus core-filament bundle from intestinal epithelial cells is a highly ordered structure containing actin and four major associated proteins. Two of these, villin and calmodulin, bind calcium ions (Kd approximately 10(-6) M) in the physiologically important range. Because ethylene glycol bis(beta-aminoethyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glenney JR Jr,Bretscher A,Weber K

更新日期：1980-11-01 00:00:00

abstract：:The tetrodotoxin binding component of the voltage-sensitive sodium channel from Electrophorus electricus electroplax was purified by using a monoclonal antibody. An impure preparation of tetrodotoxin binding component was mixed with the pure monoclonal antibody, and the immune complex so formed was isolated by affinit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakayama H,Withy RM,Raftery MA

更新日期：1982-12-01 00:00:00

abstract：:Angiosperm reproduction is characterized by alternate diploid sporophytic and haploid gametophytic generations. Gametogenesis shares similarities with that of animals except for the formation of the gametophyte, whereby haploid cells undergo several rounds of postmeiotic mitosis to form gametes and the accessory cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park GT,Frost JM,Park JS,Kim TH,Lee JS,Oh SA,Twell D,Brooks JS,Fischer RL,Choi Y

更新日期：2014-12-23 00:00:00

abstract：:Phosphorylation is a major mechanism regulating the activity of ion channels that remains poorly understood with respect to T-type calcium channels (Cav3). These channels are low voltage-activated calcium channels that play a key role in cellular excitability and various physiological functions. Their dysfunction has ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blesneac I,Chemin J,Bidaud I,Huc-Brandt S,Vandermoere F,Lory P

更新日期：2015-11-03 00:00:00

abstract：:Nitrofurans, a class of antibacterial drugs in extensive use, interferes with gene expression in a highly specific manner. While in the low dose range (0.5-25 mug/ml), 5-nitro-2-furfurylidene-1-aminohydantoin has no effect on transcription, it inhibits specifically the expression of one class of genes in translation. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herrlich P,Schweiger M

更新日期：1976-10-01 00:00:00

abstract：:We present a model for transposition that allows a choice between cointegrate formation (replicon fusion) and direct transposition. We propose that initiation of the process occurs by invasion of the target DNA by a single-stranded end of the transposable element. This leads to nicking of one of the DNA strands of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galas DJ,Chandler M

更新日期：1981-08-01 00:00:00

abstract：:In healthy adults, activation of γ-aminobutyric acid (GABA)(A) and glycine receptors inhibits neurons as a result of low intracellular chloride concentration ([Cl(-)](i)), which is maintained by the potassium-chloride cotransporter KCC2. A reduction of KCC2 expression or function is implicated in the pathogenesis of s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bos R,Sadlaoud K,Boulenguez P,Buttigieg D,Liabeuf S,Brocard C,Haase G,Bras H,Vinay L

更新日期：2013-01-02 00:00:00

abstract：:Early events in the action of 17-beta-estradiol can be studied in soluble extracts of rat uterus by exposure of the estradiol-receptor protein to a DNA-cellulose matrix. After complexing with [(3)H]estradiol, the 4S receptor protein binds to the DNA, and it can be eluted with buffer of high ionic strength as a more ti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamamoto KR,Alberts BM

更新日期：1972-08-01 00:00:00

abstract：:The mucopolysaccharidoses are a group of genetic diseases characterized by storage of incompletely degraded glycosaminoglycans. Such storage causes marked distortion of many tissues with consequent severe somatic changes and mental retardation. Storage of glycosaminoglycans results from markedly diminished activity of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dorfman A,Matalon R

更新日期：1976-02-01 00:00:00

abstract：:Various compounds that affect signal transduction regulate the relative utilization of alternative processing pathways for the beta-amyloid precursor protein (beta APP) in intact cells, increasing the production of nonamyloidogenic soluble beta APP (s beta APP) and decreasing that of amyloidogenic beta-amyloid peptide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu H,Sweeney D,Greengard P,Gandy S

更新日期：1996-04-30 00:00:00

abstract：:Dendritic spines are of major importance in information processing and memory formation in central neurons. Estradiol has been shown to induce an increase of dendritic spine density on hippocampal neurons in vivo and in vitro. The neurotrophin brain-derived neurotrophic factor (BDNF) recently has been implicated in ne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murphy DD,Cole NB,Segal M

更新日期：1998-09-15 00:00:00

abstract：:Telomerase activity was identified in extracts from several different mouse cell lines. Addition of telomeric TTAGGG repeats was specific to telomeric oligonucleotide primers and sensitive to pretreatment with RNase A. In contrast to the hundreds of repeats synthesized by the human and Tetrahymena telomerase enzymes i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prowse KR,Avilion AA,Greider CW

更新日期：1993-02-15 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for COVID-19, employs two key host proteins to gain entry and replicate within cells, angiotensin-converting enzyme 2 (ACE2) and the cell surface transmembrane protease serine 2 (TMPRSS2). TMPRSS2 was first characterized as an androgen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qiao Y,Wang XM,Mannan R,Pitchiaya S,Zhang Y,Wotring JW,Xiao L,Robinson DR,Wu YM,Tien JC,Cao X,Simko SA,Apel IJ,Bawa P,Kregel S,Narayanan SP,Raskind G,Ellison SJ,Parolia A,Zelenka-Wang S,McMurry L,Su F,Wang R,

更新日期：2020-12-11 00:00:00

abstract：:The histidine periplasmic permease of Salmonella typhimurium has been partially purified and reconstituted into proteoliposomes. In this in vitro preparation, transport activity is completely dependent on the presence of all four permease proteins (HisJ, HisQ, HisM, and HisP) and on internal ATP. The reconstituted sys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bishop L,Agbayani R Jr,Ambudkar SV,Maloney PC,Ames GF

更新日期：1989-09-01 00:00:00

abstract：:Despite their relative simplicity, bacteria have complex anatomy at the subcellular level. At the cell poles of Caulobacter crescentus, a 177-amino acid (aa) protein called PopZ self-assembles into 3D polymeric superstructures. Remarkably, we find that this assemblage interacts directly with at least eight different p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holmes JA,Follett SE,Wang H,Meadows CP,Varga K,Bowman GR

更新日期：2016-11-01 00:00:00

abstract：:Many neurotransmitters are known to regulate neuronal cell function by means of activation of cAMP-dependent protein kinase (PKA) and phosphorylation of neuronal substrate proteins, including transcription factors and ion channels. Here, we have characterized the gene expression of two isoforms of a protein kinase inh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seasholtz AF,Gamm DM,Ballestero RP,Scarpetta MA,Uhler MD

更新日期：1995-02-28 00:00:00

abstract：:We quantitatively analyzed, using laser scanning confocal microscopy, the three-dimensional structure of individual senile plaques in Alzheimer disease. We carried out the quantitative analysis using statistical methods to gain insights about the processes that govern Abeta peptide deposition. Our results show that pl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cruz L,Urbanc B,Buldyrev SV,Christie R,Gómez-Isla T,Havlin S,McNamara M,Stanley HE,Hyman BT

更新日期：1997-07-08 00:00:00

abstract：:Environmental and anthropogenic factors often drive population declines in top predators, but how their influences may combine remains unclear. Albatrosses are particularly threatened. They breed in fast-changing environments, and their extensive foraging ranges expose them to incidental mortality (bycatch) in multipl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pardo D,Forcada J,Wood AG,Tuck GN,Ireland L,Pradel R,Croxall JP,Phillips RA

更新日期：2017-12-12 00:00:00

abstract：:CD4(+) T cells producing IL-17 [T helper (Th)17], as distinct from Th1 or Th2 cells, have recently been shown to be associated with autoimmunity, but it is not entirely clear how Th17 cells are generated from naïve T cells. We demonstrate here that IL-6, but not TNF-alpha or IL-1beta, can, in combination with TGF-beta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kimura A,Naka T,Kishimoto T

更新日期：2007-07-17 00:00:00

abstract：:Endothelin, originally purified from porcine aortic endothelial cells, is widely distributed in tissues and is recognized as a product of epithelial cells, glial cells, and neurons in addition to endothelial cells. We found evidence by mRNA content and immunoreactivity that this peptide is synthesized in rat parathyro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fujii Y,Moreira JE,Orlando C,Maggi M,Aurbach GD,Brandi ML,Sakaguchi K

更新日期：1991-05-15 00:00:00

abstract：:Myosin V is an unconventional myosin proposed to be processive on actin filaments, analogous to kinesin on a microtubule [Mehta, A. D., et al. (1999) Nature (London) 400, 590-593]. To ascertain the unique properties of myosin V that permit processivity, we undertook a detailed kinetic analysis of the myosin V motor. W...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De La Cruz EM,Wells AL,Rosenfeld SS,Ostap EM,Sweeney HL

更新日期：1999-11-23 00:00:00

abstract：:KCNE β-subunits assemble with and modulate the properties of voltage-gated K+ channels. In the heart, KCNE1 associates with the α-subunit KCNQ1 to generate the slowly activating, voltage-dependent potassium current (IKs) in the heart that controls the repolarization phase of cardiac action potentials. By contrast, in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barro-Soria R,Ramentol R,Liin SI,Perez ME,Kass RS,Larsson HP

更新日期：2017-08-29 00:00:00

abstract：:Progressive amyloid deposition in senile plaques and cortical blood vessels may play a central role in the pathogenesis of Alzheimer disease. We have used x-ray diffraction and electron microscopy to study the molecular organization and morphology of macromolecular assemblies formed by three synthetic peptides homolog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirschner DA,Inouye H,Duffy LK,Sinclair A,Lind M,Selkoe DJ

更新日期：1987-10-01 00:00:00

abstract：:Understanding the mechanism of action of compounds capable of inhibiting amyloid-fibril formation is critical to the development of potential therapeutics against protein-misfolding diseases. A fundamental challenge for progress is the range of possible target species and the disparate timescales involved, since the a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Michaels TCT,Šarić A,Meisl G,Heller GT,Curk S,Arosio P,Linse S,Dobson CM,Vendruscolo M,Knowles TPJ

更新日期：2020-09-29 00:00:00

abstract：:Genetic variation in the serotonin transporter gene (SLC6A4) is associated with vulnerability to affective disorders and pharmacotherapy efficacy. We recently identified sequence polymorphisms in the common marmoset SLC6A4 repeat region (AC/C/G and CT/T/C) associated with individual differences in anxiety-like trait, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Santangelo AM,Sawiak SJ,Fryer T,Hong Y,Shiba Y,Clarke HF,Riss PJ,Ferrari V,Tait R,Suckling J,Aigbirhio FI,Roberts AC

更新日期：2019-07-16 00:00:00

abstract：:The FMS gene encodes the functional cell surface receptor for colony-stimulating factor 1, the macrophage- and monocyte-specific growth factor. Codons 969 and 301 have been identified as potentially involved in promoting the transforming activity of FMS. Mutations at codon 301 are believed to lead to neoplastic transf...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ridge SA,Worwood M,Oscier D,Jacobs A,Padua RA

更新日期：1990-02-01 00:00:00