Abstract:
:Many neurotransmitters are known to regulate neuronal cell function by means of activation of cAMP-dependent protein kinase (PKA) and phosphorylation of neuronal substrate proteins, including transcription factors and ion channels. Here, we have characterized the gene expression of two isoforms of a protein kinase inhibitor (PKI) specific for PKA in mouse brain by RNase protection and in situ hybridization histochemistry. The studies demonstrate that the PKI alpha isoform is abundant in many regions of the adult mouse brain but particularly in cerebellum, hypothalamus, hippocampus, and cortex. In contrast, PKI beta is present at much lower levels in most brain regions but is found in significant amounts in the cerebellum, as well as in distinct nuclei within the pons, medulla, and hypothalamus. These results are consistent with a regulatory role of endogenous PKI in PKA-mediated signal transduction in brain and suggest differential functions for the two isoforms of PKI within the central nervous system.
journal_name
Proc Natl Acad Sci U S Aauthors
Seasholtz AF,Gamm DM,Ballestero RP,Scarpetta MA,Uhler MDdoi
10.1073/pnas.92.5.1734subject
Has Abstractpub_date
1995-02-28 00:00:00pages
1734-8issue
5eissn
0027-8424issn
1091-6490journal_volume
92pub_type
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