当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Mutations in the HIV-1 envelope glycoprotein can broadly rescue blocks at multiple steps in the virus replication cycle.

Mutations in the HIV-1 envelope glycoprotein can broadly rescue blocks at multiple steps in the virus replication cycle.

Abstract：

:The p6 domain of HIV-1 Gag contains highly conserved peptide motifs that recruit host machinery to sites of virus assembly, thereby promoting particle release from the infected cell. We previously reported that mutations in the YPXnL motif of p6, which binds the host protein Alix, severely impair HIV-1 replication. Propagation of the p6-Alix binding site mutants in the Jurkat T cell line led to the emergence of viral revertants containing compensatory mutations not in Gag but in Vpu and the envelope (Env) glycoprotein subunits gp120 and gp41. The Env compensatory mutants replicate in Jurkat T cells and primary human peripheral blood mononuclear cells, despite exhibiting severe defects in cell-free particle infectivity and Env-mediated fusogenicity. Remarkably, the Env compensatory mutants can also rescue a replication-delayed integrase (IN) mutant, and exhibit reduced sensitivity to the IN inhibitor Dolutegravir (DTG), demonstrating that they confer a global replication advantage. In addition, confirming the ability of Env mutants to confer escape from DTG, we performed de novo selection for DTG resistance and observed resistance mutations in Env. These results identify amino acid substitutions in Env that confer broad escape from defects in virus replication imposed by either mutations in the HIV-1 genome or by an antiretroviral inhibitor. We attribute this phenotype to the ability of the Env mutants to mediate highly efficient cell-to-cell transmission, resulting in an increase in the multiplicity of infection. These findings have broad implications for our understanding of Env function and the evolution of HIV-1 drug resistance.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Van Duyne R,Kuo LS,Pham P,Fujii K,Freed EO

doi

10.1073/pnas.1820333116

subject

Has Abstract

pub_date

2019-04-30 00:00:00

pages

9040-9049

issue

18

eissn

0027-8424

issn

1091-6490

pii

1820333116

journal_volume

116

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Algorithm for cellular reprogramming.

    abstract：:The day we understand the time evolution of subcellular events at a level of detail comparable to physical systems governed by Newton's laws of motion seems far away. Even so, quantitative approaches to cellular dynamics add to our understanding of cell biology. With data-guided frameworks we can develop better predic...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1712350114

    authors： Ronquist S,Patterson G,Muir LA,Lindsly S,Chen H,Brown M,Wicha MS,Bloch A,Brockett R,Rajapakse I

    更新日期：2017-11-07 00:00:00

  • High prevalence of serum antibodies reacting with simian virus 40 capsid protein mimotopes in patients affected by malignant pleural mesothelioma.

    abstract：:Human malignant pleural mesothelioma (MPM) is considered a rare tumor, but recent estimations indicate that one-quarter million people will die of this neoplasm in Europe in the next three decades. The mineral asbestos is considered the main causative agent of this neoplasm. MPM is largely unresponsive to conventional...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1213238109

    authors： Mazzoni E,Corallini A,Cristaudo A,Taronna A,Tassi G,Manfrini M,Comar M,Bovenzi M,Guaschino R,Vaniglia F,Magnani C,Casali F,Rezza G,Barbanti-Brodano G,Martini F,Tognon MG

    更新日期：2012-10-30 00:00:00

  • Different mutation signatures in DNA polymerase eta- and MSH6-deficient mice suggest separate roles in antibody diversification.

    abstract：:Hypermutation in immunoglobulin genes produces a high frequency of substitutions of all four bases, which are likely generated by low-fidelity DNA polymerases. Indeed, humans deficient for DNA polymerase (pol) eta have decreased substitutions of A.T base pairs in variable and switch regions. To study the role of pol e...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0501852102

    authors： Martomo SA,Yang WW,Wersto RP,Ohkumo T,Kondo Y,Yokoi M,Masutani C,Hanaoka F,Gearhart PJ

    更新日期：2005-06-14 00:00:00

  • Effect of oil spills on infant mortality in Nigeria.

    abstract：:Oil spills can lead to irreversible environmental degradation and are a potential hazard to human health. We study how onshore oil spills affect neonatal and infant mortality by combining spatial data from the Nigerian Oil Spill Monitor with Demographic and Health Surveys. To identify a causal effect, we compare sibli...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1818303116

    authors： Bruederle A,Hodler R

    更新日期：2019-03-19 00:00:00

  • Independent circuits in the basal ganglia for the evaluation and selection of actions.

    abstract：:The basal ganglia are critical for selecting actions and evaluating their outcome. Although the circuitry for selection is well understood, how these nuclei evaluate the outcome of actions is unknown. Here, we show in lamprey that a separate evaluation circuit, which regulates the habenula-projecting globus pallidus (...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1314815110

    authors： Stephenson-Jones M,Kardamakis AA,Robertson B,Grillner S

    更新日期：2013-09-17 00:00:00

  • Regulatory T cells control tolerogenic versus autoimmune response to sperm in vasectomy.

    abstract：:Vasectomy is a well accepted global contraceptive approach frequently associated with epididymal granuloma and sperm autoantibody formation. To understand the long-term sequelae of vasectomy, we investigated the early immune response in vasectomized mice. Vasectomy leads to rapid epithelial cell apoptosis and necrosis...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1017615108

    authors： Wheeler K,Tardif S,Rival C,Luu B,Bui E,Del Rio R,Teuscher C,Sparwasser T,Hardy D,Tung KS

    更新日期：2011-05-03 00:00:00

  • Similar idiotypic specificities in immunoglobulin fractions with different antibody functions or even without detectable antibody function.

    abstract：:IDIOTYPY HAS BEEN STUDIED IN ANTIBODIES AGAINST A PROTEIN ANTIGEN: hen ovalbumin. Various fractions of antisera to hen ovalbumin have been compared from the standpoint of the idiotypic specificities found on the immunoglobulins that they contain. Idiotypic specificities were found to be common to (a) antibodies that w...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.68.10.2616

    authors： Oudin J,Cazenave PA

    更新日期：1971-10-01 00:00:00

  • Ferredoxin and ferredoxin-heme maquettes.

    abstract：:A 16-amino acid residue peptide derived from a consensus motif of natural ferredoxins incorporates a tetranuclear iron sulfur cluster under physiological conditions. Successful assembly of the [4Fe-4S]2+/1+ cluster within a monomeric peptide was demonstrated using size exclusion chromatography, UV-visible, visible CD,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.26.15041

    authors： Gibney BR,Mulholland SE,Rabanal F,Dutton PL

    更新日期：1996-12-24 00:00:00

  • Homozygously deleted gene DACH1 regulates tumor-initiating activity of glioma cells.

    abstract：:Loss or reduction in function of tumor suppressor genes contributes to tumorigenesis. Here, by allelic DNA copy number analysis using single-nucleotide polymorphism genotyping array and mass spectrometry, we report homozygous deletion in glioblastoma multiformes at chromosome 13q21, where DACH1 gene is located. We fou...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0906930108

    authors： Watanabe A,Ogiwara H,Ehata S,Mukasa A,Ishikawa S,Maeda D,Ueki K,Ino Y,Todo T,Yamada Y,Fukayama M,Saito N,Miyazono K,Aburatani H

    更新日期：2011-07-26 00:00:00

  • Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach.

    abstract：:Escherichia coli is a model laboratory bacterium, a species that is widely distributed in the environment, as well as a mutualist and pathogen in its human hosts. As such, E. coli represents an attractive organism to study how environment impacts microbial genome structure and function. Uropathogenic E. coli (UPEC) mu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0600938103

    authors： Chen SL,Hung CS,Xu J,Reigstad CS,Magrini V,Sabo A,Blasiar D,Bieri T,Meyer RR,Ozersky P,Armstrong JR,Fulton RS,Latreille JP,Spieth J,Hooton TM,Mardis ER,Hultgren SJ,Gordon JI

    更新日期：2006-04-11 00:00:00

  • Detection of secondary binding sites in proteins using fragment screening.

    abstract：:Proteins need to be tightly regulated as they control biological processes in most normal cellular functions. The precise mechanisms of regulation are rarely completely understood but can involve binding of endogenous ligands and/or partner proteins at specific locations on a protein that can modulate function. Often,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1518946112

    authors： Ludlow RF,Verdonk ML,Saini HK,Tickle IJ,Jhoti H

    更新日期：2015-12-29 00:00:00

  • Phylogenetic analysis of rbcL sequences identifies Acorus calamus as the primal extant monocotyledon.

    abstract：:The identity of the oldest lineage of monocotyledons is a subject of debate. Alternative interpretations of morphological homologies are variously consistent with proposals that species of Alismatanae, Dioscoreales, or Melanthiales were the earliest descendants of the first monocotyledons. We present phylogenetic anal...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.10.4641

    authors： Duvall MR,Learn GH Jr,Eguiarte LE,Clegg MT

    更新日期：1993-05-15 00:00:00

  • S-nitrosylation of proteins with nitric oxide: synthesis and characterization of biologically active compounds.

    abstract：:Endothelium-derived relaxing factor (EDRF) activity has been attributed to the highly labile nitric oxide radical (NO). In view of the fact that the plasma and cellular milieux contain reactive species that can rapidly inactivate NO, it has been postulated that NO is stabilized by a carrier molecule that preserves its...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.1.444

    authors： Stamler JS,Simon DI,Osborne JA,Mullins ME,Jaraki O,Michel T,Singel DJ,Loscalzo J

    更新日期：1992-01-01 00:00:00

  • Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion.

    abstract：:An emergent viral pathogen termed severe fever with thrombocytopenia syndrome virus (SFTSV) is responsible for thousands of clinical cases and associated fatalities in China, Japan, and South Korea. Akin to other phleboviruses, SFTSV relies on a viral glycoprotein, Gc, to catalyze the merger of endosomal host and vira...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1603827113

    authors： Halldorsson S,Behrens AJ,Harlos K,Huiskonen JT,Elliott RM,Crispin M,Brennan B,Bowden TA

    更新日期：2016-06-28 00:00:00

  • Sleep deprivation impairs precision of waggle dance signaling in honey bees.

    abstract：:Sleep is essential for basic survival, and insufficient sleep leads to a variety of dysfunctions. In humans, one of the most profound consequences of sleep deprivation is imprecise or irrational communication, demonstrated by degradation in signaling as well as in receiving information. Communication in nonhuman anima...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1009439108

    authors： Klein BA,Klein A,Wray MK,Mueller UG,Seeley TD

    更新日期：2010-12-28 00:00:00

  • Electron transfer pathways in a multiheme cytochrome MtrF.

    abstract：:In MtrF, an outer-membrane multiheme cytochrome, the 10 heme groups are arranged in heme binding domains II and IV along the pseudo-C2 axis, forming the electron transfer (ET) pathways. Previous reports based on molecular dynamics simulations showed that the redox potential (Em) values for the heme pairs located in sy...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1617615114

    authors： Watanabe HC,Yamashita Y,Ishikita H

    更新日期：2017-03-14 00:00:00

  • A natural classification of the basic helix-loop-helix class of transcription factors.

    abstract：:A natural (evolutionary) classification is provided for 242 basic helix-loop-helix (bHLH) motif-containing proteins. Phylogenetic analyses of amino acid sequences describe the patterns of evolutionary change within the motif and delimit evolutionary lineages. These evolutionary lineages represent well known functional...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.10.5172

    authors： Atchley WR,Fitch WM

    更新日期：1997-05-13 00:00:00

  • Telomerase activity in the regenerative basal layer of the epidermis inhuman skin and in immortal and carcinoma-derived skin keratinocytes.

    abstract：:Cellular senescence is defined by the limited proliferative capacity of normal cultured cells. Immortal cells overcome this regulation and proliferate indefinitively. One step in the immortalization process may be reactivation of telomerase activity, a ribonucleoprotein complex, which, by de novo synthesized telomeric...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.13.6476

    authors： Härle-Bachor C,Boukamp P

    更新日期：1996-06-25 00:00:00

  • Protein complex stoichiometry and expression dynamics of transcription factors modulate stem cell division.

    abstract：:Stem cells divide and differentiate to form all of the specialized cell types in a multicellular organism. In the Arabidopsis root, stem cells are maintained in an undifferentiated state by a less mitotically active population of cells called the quiescent center (QC). Determining how the QC regulates the surrounding ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2002166117

    authors： Clark NM,Fisher AP,Berckmans B,Van den Broeck L,Nelson EC,Nguyen TT,Bustillo-Avendaño E,Zebell SG,Moreno-Risueno MA,Simon R,Gallagher KL,Sozzani R

    更新日期：2020-06-30 00:00:00

  • NusA interferes with interactions between the nascent RNA and the C-terminal domain of the alpha subunit of RNA polymerase in Escherichia coli transcription complexes.

    abstract：:The effects of NusA on the RNA polymerase contacts made by nucleotides at internal positions in the nascent RNA in Escherichia coli transcription complexes were analyzed by using the photocrosslinking nucleotide analog 5-[(4-azidophenacyl) thio]-UMP. It was placed at nucleotides between +6 and +15 in RNA transcribed f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.11.5012

    authors： Liu K,Hanna MM

    更新日期：1995-05-23 00:00:00

  • Fungal farming in a snail.

    abstract：:Mutualisms between fungi and fungus-growing animals are model systems for studying coevolution and complex interactions between species. Fungal growing behavior has enabled cultivating animals to rise to major ecological importance, but evolution of farming symbioses is thought to be restricted to three terrestrial in...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2535227100

    authors： Silliman BR,Newell SY

    更新日期：2003-12-23 00:00:00

  • Complex dynamics of multilocus systems subjected to cyclical selection.

    abstract：:Earlier we have shown that oscillations with a long period ("supercycles") may arise in two-locus systems experiencing cyclical selection with a short period. However, this mode of complex limiting behavior appeared to be possible for narrow ranges of parameters. Here we demonstrate that a multilocus system subjected ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.13.6532

    authors： Kirzhner VM,Korol AB,Nevo E

    更新日期：1996-06-25 00:00:00

  • RNAi-mediated silencing of estrogen receptor {alpha} in the ventromedial nucleus of hypothalamus abolishes female sexual behaviors.

    abstract：:Estrogen receptor alpha (ERalpha) plays a major role in the regulation of neuroendocrine functions and behaviors by estrogens. Although the generation of ERalpha knockout mice advanced our knowledge of ERalpha functions, gene deletion using this method is global and potentially confounded by developmental consequences...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0603045103

    authors： Musatov S,Chen W,Pfaff DW,Kaplitt MG,Ogawa S

    更新日期：2006-07-05 00:00:00

  • Role of excited electronic states in the high-pressure amorphization of benzene.

    abstract：:High-pressure methods are increasingly used to produce new dense materials with unusual properties. Increasing efforts to understand the reaction mechanisms at the microscopic level, to set up and optimize synthetic approaches, are currently directed at carbon-based solids. A fundamental, but still unsolved, question ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0802269105

    authors： Citroni M,Bini R,Foggi P,Schettino V

    更新日期：2008-06-03 00:00:00

  • Sigma subunit of Escherichia coli RNA polymerase affects the function of lambda N gene.

    abstract：:A new class of Escherichia coli mutants, referred to as grn, has been isolated by localized mutagenesis. These mutations affect the sigma subunit of DNA-dependent RNA polymerase (ribonucleoside 5'-triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) by abolishing the expression of the lambda N gene, and they are close...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.9.4593

    authors： Nakamura Y,Kurihara T,Saito H,Uchida H

    更新日期：1979-09-01 00:00:00

  • VHL loss in renal cell carcinoma leads to up-regulation of CUB domain-containing protein 1 to stimulate PKC{delta}-driven migration.

    abstract：:A common genetic mutation found in clear cell renal cell carcinoma (CC-RCC) is the loss of the von Hippel-Lindau (VHL) gene, which results in stabilization of hypoxia-inducible factors (HIFs), and contributes to cancer progression and metastasis. CUB-domain-containing protein 1 (CDCP1) was shown to promote metastasis ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1011777108

    authors： Razorenova OV,Finger EC,Colavitti R,Chernikova SB,Boiko AD,Chan CK,Krieg A,Bedogni B,LaGory E,Weissman IL,Broome-Powell M,Giaccia AJ

    更新日期：2011-02-01 00:00:00

  • Reentrant spiral waves of spreading depression cause macular degeneration in hypoglycemic chicken retina.

    abstract：:Spreading depression (SD), a slow diffusion-mediated self-sustained wave of depolarization that severely disrupts neuronal function, has been implicated as a cause of cellular injury in a number of central nervous system pathologies, including blind spots in the retina. Here we show that in the hypoglycemic chicken re...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1121111109

    authors： Yu Y,Santos LM,Mattiace LA,Costa ML,Ferreira LC,Benabou K,Kim AH,Abrahams J,Bennett MV,Rozental R

    更新日期：2012-02-14 00:00:00

  • Regulation of the in vitro antibody response by neuroendocrine hormones.

    abstract：:Treatment of lymphocytes with inducers of interferon alpha (IFN-alpha) results in the production of corticotropin (ACTH) and endorphin-like activities. The pro-opiomelanocortin-derived hormones ACTH and alpha-, beta-, and gamma-endorphin and the structurally related hormones [Leu]- and [Met]enkephalin were therefore t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.13.4171

    authors： Johnson HM,Smith EM,Torres BA,Blalock JE

    更新日期：1982-07-01 00:00:00

  • Decomposition of the linking number of a closed ribbon: A problem from molecular biology.

    abstract：:A closed duplex DNA molecule relaxed and containing nucleosomes has a different linking number from the same molecule relaxed and without nucleosomes. What does this say about the structure of the nucleosome? A mathematical study of this question is made, representing the DNA molecule by a ribbon. It is shown that the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.8.3557

    authors： Fuller FB

    更新日期：1978-08-01 00:00:00

  • A pex1 missense mutation improves peroxisome function in a subset of Arabidopsis pex6 mutants without restoring PEX5 recycling.

    abstract：:Peroxisomes are eukaryotic organelles critical for plant and human development because they house essential metabolic functions, such as fatty acid β-oxidation. The interacting ATPases PEX1 and PEX6 contribute to peroxisome function by recycling PEX5, a cytosolic receptor needed to import proteins targeted to the pero...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1721279115

    authors： Gonzalez KL,Ratzel SE,Burks KH,Danan CH,Wages JM,Zolman BK,Bartel B

    更新日期：2018-04-03 00:00:00