Different mutation signatures in DNA polymerase eta- and MSH6-deficient mice suggest separate roles in antibody diversification.

abstract：:A major barrier to transitions to environmental sustainability is that consumers lack information about the full environmental footprints of their purchases. Sellers' incentives do not support reducing the footprints unless customers have such information and are willing to act on it. We explore the potential of moder...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Isley SC,Stern PC,Carmichael SP,Joseph KM,Arent DJ

更新日期：2016-08-30 00:00:00

abstract：:Photosystem I (PSI) reaction centers (RCs) of the thermophilic cyanobacterium Mastigocladus laminosus were purified and characterized. The PSI RC was obtained in two forms, monomeric and trimeric. The two forms contained the same number of pigments per P700 and displayed similar photochemical activities. The two forms...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Almog O,Shoham G,Michaeli D,Nechushtai R

更新日期：1991-06-15 00:00:00

abstract：:Dyskeratosis congenita is a rare inherited disorder characterized by abnormal skin manifestations. Morbidity and mortality from this disease is usually due to bone marrow failure, but idiopathic pulmonary fibrosis and an increased cancer predisposition also occur. Families with autosomal dominant dyskeratosis congenit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Armanios M,Chen JL,Chang YP,Brodsky RA,Hawkins A,Griffin CA,Eshleman JR,Cohen AR,Chakravarti A,Hamosh A,Greider CW

更新日期：2005-11-01 00:00:00

abstract：:Fibronectin (FN) forms the primitive fibrillar matrix in both embryos and healing wounds. To study the matrix in living cell cultures, we have constructed a cell line that secretes FN molecules chimeric with green fluorescent protein. These FN-green fluorescent protein molecules were assembled into a typical matrix th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohashi T,Kiehart DP,Erickson HP

更新日期：1999-03-02 00:00:00

abstract：:During performance of attention-demanding cognitive tasks, certain regions of the brain routinely increase activity, whereas others routinely decrease activity. In this study, we investigate the extent to which this task-related dichotomy is represented intrinsically in the resting human brain through examination of s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fox MD,Snyder AZ,Vincent JL,Corbetta M,Van Essen DC,Raichle ME

更新日期：2005-07-05 00:00:00

abstract：:We recently have introduced the term vasculogenic mimicry to describe the unique ability of aggressive melanoma tumor cells to form tubular structures and patterned networks in three-dimensional culture, which "mimics" embryonic vasculogenic networks formed by differentiating endothelial cells. In the current study, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hendrix MJ,Seftor EA,Meltzer PS,Gardner LM,Hess AR,Kirschmann DA,Schatteman GC,Seftor RE

更新日期：2001-07-03 00:00:00

abstract：:Albatrosses (Diomedeidae) do not occur in the North Atlantic Ocean today except as vagrants, although five species were present in the early Pliocene. No fossil breeding sites of albatrosses were known previously. The timing of extinction of albatrosses in the North Atlantic was likewise unknown. Deposits that formed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olson SL,Hearty PJ

更新日期：2003-10-28 00:00:00

abstract：:The timing of neocortical regional specification was examined using a monoclonal antibody, designated PC3.1, that binds a 29-kDa polypeptide and recognizes a neuronal subpopulation located in the lateral but not dorsomedial neocortex in the rat. When lateral cortical tissue fragments at embryonic days 12 and 16 were m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arimatsu Y,Miyamoto M,Nihonmatsu I,Hirata K,Uratani Y,Hatanaka Y,Takiguchi-Hayashi K

更新日期：1992-10-01 00:00:00

abstract：:Individuals homozygous for the C-C chemokine receptor type 5 gene with 32-bp deletions (CCR5Δ32) are resistant to HIV-1 infection. In this study, we generated induced pluripotent stem cells (iPSCs) homozygous for the naturally occurring CCR5Δ32 mutation through genome editing of wild-type iPSCs using a combination of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ye L,Wang J,Beyer AI,Teque F,Cradick TJ,Qi Z,Chang JC,Bao G,Muench MO,Yu J,Levy JA,Kan YW

更新日期：2014-07-01 00:00:00

abstract：:The mitochondrial uniporter is a Ca2+-channel complex resident within the organelle's inner membrane. In mammalian cells the uniporter's activity is regulated by Ca2+ due to concerted action of MICU1 and MICU2, two paralogous, but functionally distinct, EF-hand Ca2+-binding proteins. Here we present the X-ray structur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kamer KJ,Jiang W,Kaushik VK,Mootha VK,Grabarek Z

更新日期：2019-02-26 00:00:00

abstract：:Genetic analysis has revealed that the accumulation of several chloroplast mRNAs of the green alga Chlamydomonas reinhardtii requires specific nucleus-encoded functions. To gain insight into this process, we have cloned the nuclear gene encoding the Mbb1 factor by genomic rescue of a mutant specifically deficient in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vaistij FE,Boudreau E,Lemaire SD,Goldschmidt-Clermont M,Rochaix JD

更新日期：2000-12-19 00:00:00

abstract：:The Wiskott-Aldrich syndrome protein (WASp) plays a major role in coupling T cell antigen receptor (TCR) stimulation to induction of actin cytoskeletal changes required for T cell activation. Here, we report that WASp inducibly binds the sorting nexin 9 (SNX9) in T cells and that WASp, SNX9, p85, and CD28 colocalize w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Badour K,McGavin MK,Zhang J,Freeman S,Vieira C,Filipp D,Julius M,Mills GB,Siminovitch KA

更新日期：2007-01-30 00:00:00

abstract：:Homeostasis is indispensable to counteract the destabilizing effects of Hebbian plasticity. Although it is commonly assumed that homeostasis modulates synaptic strength, membrane excitability, and firing rates, its role at the neural circuit and network level is unknown. Here, we identify changes in higher-order netwo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu YK,Hengen KB,Turrigiano GG,Gjorgjieva J

更新日期：2020-09-29 00:00:00

abstract：:A variety of agricultural plant species, including corn, respond to insect herbivore damage by releasing large quantities of volatile compounds and, as a result, become highly attractive to parasitic wasps that attack the herbivores. An elicitor of plant volatiles, N-(17-hydroxylinolenoyl)-L-glutamine, named volicitin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paré PW,Alborn HT,Tumlinson JH

更新日期：1998-11-10 00:00:00

abstract：:Old noninbred fly mortality decreases according to the inverse linear law and reduces to a single suborder-specific age. Relative child mortality (the mortality at a given age related to the mortality at 10 years) from 1 mo to 11 years is the same with 8% mean accuracy for all humans, independent of race, country, sex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Azbel' MY

更新日期：1998-07-21 00:00:00

abstract：:An automatic protein design procedure is used to select amino acid sequences that optimize the folding free energy function for a given protein. The only information used in designing the sequences is a set of known backbone structures for each protein, a rotamer library, and a well established classical empirical for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jaramillo A,Wernisch L,Héry S,Wodak SJ

更新日期：2002-10-15 00:00:00

abstract：:In target tissue extracts, heat shock protein hsp90 has been found associated to all unliganded steroid receptors. Modulation of important functions of these receptors, including prevention of DNA binding and optimization of transcriptional activity, has been attributed to hsp90. However no unequivocal in vivo demonst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang KI,Devin J,Cadepond F,Jibard N,Guiochon-Mantel A,Baulieu EE,Catelli MG

更新日期：1994-01-04 00:00:00

abstract：:By analogy with receptor-mediated endocytosis, clathrin-coated membrane segments at locations other than at the plasma membrane have been implicated in intracellular membrane transport/sorting. The crucial role of the Golgi apparatus in these processes prompted us to study the distribution at this level of clathrin-im...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orci L,Ravazzola M,Amherdt M,Louvard D,Perrelet A

更新日期：1985-08-01 00:00:00

abstract：:CD28 is a 44-kDa glycoprotein expressed as a homodimer on the surface of a major subset of human T cells. Previous studies have demonstrated that the binding of monoclonal antibodies to the CD28 surface antigen can augment the proliferation of purified human T cells stimulated with suboptimal doses of mitogens or anti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thompson CB,Lindsten T,Ledbetter JA,Kunkel SL,Young HA,Emerson SG,Leiden JM,June CH

更新日期：1989-02-01 00:00:00

abstract：:Rat liver polysomal RNA was translated in the rabbit reticulocyte lysate and in the wheat germ cell-free protein-synthesizing systems, using [(35)S]methionine as label. The catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase, EC 1.11.1.6) that was synthesized was isolated by immunoprecipitation and characteri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robbi M,Lazarow PB

更新日期：1978-09-01 00:00:00

abstract：:Mouse serosal mast cells (SMCs) and Kirsten sarcoma virus-immortalized mast cells store large amounts of mast cell carboxypeptidase A and serine proteases in their secretory granules. Secretory granule proteins from 2.6 x 10(6) purified SMCs were separated by NaDodSO4/PAGE, trans-blotted to poly(vinylidine difluoride)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reynolds DS,Stevens RL,Lane WS,Carr MH,Austen KF,Serafin WE

更新日期：1990-04-01 00:00:00

abstract：:A central question in stem cell biology is whether organ homeostasis is maintained in adult organs through undifferentiated stem cells or self-duplication of specialized cell populations. To address this issue in the exocrine pancreas we analyzed the Bmi1-labeled cell lineage of pancreatic acinar cells. Previously, we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sangiorgi E,Capecchi MR

更新日期：2009-04-28 00:00:00

abstract：:The Tat protein coded by human immunodeficiency virus (HIV) is a strong activator of viral gene expression from the long terminal repeat (LTR). It appears that Tat-mediated trans-activation of the HIV LTR is predominantly a transcriptional event. It has been reported that Tat acts at the level of both transcriptional ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kamine J,Subramanian T,Chinnadurai G

更新日期：1991-10-01 00:00:00

abstract：:The use of carbon dioxide as a renewable and environmentally friendly source of carbon in organic synthesis is a highly attractive approach, but its real world applications remain a great challenge. The major obstacles for commercialization of most current protocols are their low catalytic performances, harsh reaction...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu D,Zhang Y

更新日期：2010-11-23 00:00:00

abstract：:We report an approach for studying protein-induced DNA bends in solution that is based on measuring the sizes of circular DNA molecules by using two-dimensional gel electrophoresis. These circular fragments are obtained by ligating short synthetic oligonucleotides containing a protein-recognition region in the presenc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lyubchenko Y,Shlyakhtenko L,Chernov B,Harrington RE

更新日期：1991-06-15 00:00:00

abstract：:Solar emission produces copious nitrosonium ions (NO(+)) in the D layer of the ionosphere, 60 to 90 km above the Earth's surface. NO(+) is believed to transfer its charge to water clusters in that region, leading to the formation of gaseous nitrous acid (HONO) and protonated water cluster. The dynamics of this reactio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He R,Li L,Zhong J,Zhu C,Francisco JS,Zeng XC

更新日期：2016-04-26 00:00:00

abstract：:Sulfur assimilation is an evolutionarily conserved pathway that plays an essential role in cellular and metabolic processes, including sulfation, amino acid biosynthesis, and organismal development. We report that loss of a key enzymatic component of the pathway, bisphosphate 3'-nucleotidase (Bpnt1), in mice, both who...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hudson BH,Hale AT,Irving RP,Li S,York JD

更新日期：2018-03-20 00:00:00

abstract：:Retrograde axonal transport plays an important role in the maintenance of neuronal functions, but the mechanism is poorly defined partly because the constituents of the retrograde transport system and their interactions have yet to be elucidated. Of special interest is how dynein/dynactin motor proteins interact with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu JJ,Ding J,Wu C,Bhagavatula P,Cui B,Chu S,Mobley WC,Yang Y

更新日期：2007-02-13 00:00:00

abstract：:The progesterone receptor (PGR) is a ligand-activated transcription factor with key roles in the regulation of female fertility. Much has been learned of the actions of PGR signaling through the use of pharmacologic inhibitors and genetic manipulation, using mouse mutagenesis. Characterization of rats with a null muta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kubota K,Cui W,Dhakal P,Wolfe MW,Rumi MA,Vivian JL,Roby KF,Soares MJ

更新日期：2016-04-12 00:00:00

abstract：:Despite enormous body plan variation, genes regulating embryonic development are highly conserved. Here, we probe the mechanisms that predispose ancient regulatory genes to reutilization and diversification rather than evolutionary loss. The Hox gene fushi tarazu (ftz) arose as a homeotic gene but functions as a pair-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heffer A,Xiang J,Pick L

更新日期：2013-02-05 00:00:00