Abstract:
:Retrograde axonal transport plays an important role in the maintenance of neuronal functions, but the mechanism is poorly defined partly because the constituents of the retrograde transport system and their interactions have yet to be elucidated. Of special interest is how dynein/dynactin motor proteins interact with membrane cargoes. Here, we report that an endosomal vesicle protein, termed retrolinkin, functions as a receptor tethering vesicles to dynein/dynactin through BPAG1n4. Retrolinkin, a membrane protein highly enriched in neuronal endosomes, binds directly to BPAG1n4. Deletion of retrolinkin membrane-association domains disrupts retrograde vesicular transport, recapitulating the BPAG1 null phenotype. We propose that retrolinkin acts with BPAG1n4 to specifically regulate retrograde axonal transport. Our work lays the foundation for understanding fundamental issues of axonal transport and provides insights into the molecular mechanisms underlying human neurodegenerative disorders.
journal_name
Proc Natl Acad Sci U S Aauthors
Liu JJ,Ding J,Wu C,Bhagavatula P,Cui B,Chu S,Mobley WC,Yang Ydoi
10.1073/pnas.0602222104subject
Has Abstractpub_date
2007-02-13 00:00:00pages
2223-8issue
7eissn
0027-8424issn
1091-6490pii
0602222104journal_volume
104pub_type
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