当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Telomerase activity in the regenerative basal layer of the epidermis inhuman skin and in immortal and carcinoma-derived skin keratinocytes.

Telomerase activity in the regenerative basal layer of the epidermis inhuman skin and in immortal and carcinoma-derived skin keratinocytes.

Abstract：

:Cellular senescence is defined by the limited proliferative capacity of normal cultured cells. Immortal cells overcome this regulation and proliferate indefinitively. One step in the immortalization process may be reactivation of telomerase activity, a ribonucleoprotein complex, which, by de novo synthesized telomeric TTAGGG repeats, can prevent shortening of the telomeres. Here we show that immortal human skin keratinocytes, irrespective of whether they were immortalized by simian virus 40, human papillomavirus 16, or spontaneously, as well as cell lines established from human skin squamous cell carcinomas exhibit telomerase activity. Unexpectedly, four of nine samples of intact human skin also were telomerase positive. By dissecting the skin we could show that the dermis and cultured dermal fibroblasts were telomerase negative. The epidermis and cultured skin keratinocytes, however, reproducibly exhibited enzyme activity. By separating different cell layers of the epidermis this telomerase activity could be assigned to the proliferative basal cells. Thus, in addition to hematopoietic cells, the epidermis, another example of a permanently regenerating human tissue, provides a further exception of the hypothesis that all normal human somatic tissues are telomerase deficient. Instead, these data suggest that in addition to contributing to the permanent proliferation capacity of immortal and tumor-derived keratinocytes, telomerase activity may also play a similar role in the lifetime regenerative capacity of normal epidermis in vivo.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Härle-Bachor C,Boukamp P

doi

10.1073/pnas.93.13.6476

subject

Has Abstract

pub_date

1996-06-25 00:00:00

pages

6476-81

issue

13

eissn

0027-8424

issn

1091-6490

journal_volume

93

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Hyperphosphorylation regulates the activity of SREBP1 during mitosis.

    abstract：:The sterol regulatory element-binding protein (SREBP) family of transcription factors controls the biosynthesis of cholesterol and other lipids, and lipid synthesis is critical for cell growth and proliferation. We were, therefore, interested in the expression and activity of SREBPs during the cell cycle. We found tha...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0501494102

    authors： Bengoechea-Alonso MT,Punga T,Ericsson J

    更新日期：2005-08-16 00:00:00

  • Defective decidualization during and after severe preeclampsia reveals a possible maternal contribution to the etiology.

    abstract：:In preeclampsia (PE), cytotrophoblast (CTB) invasion of the uterus and spiral arteries is often shallow. Thus, the placenta's role has been a focus. In this study, we tested the hypothesis that decidual defects are an important determinant of the placental phenotype. We isolated human endometrial stromal cells from no...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1706546114

    authors： Garrido-Gomez T,Dominguez F,Quiñonero A,Diaz-Gimeno P,Kapidzic M,Gormley M,Ona K,Padilla-Iserte P,McMaster M,Genbacev O,Perales A,Fisher SJ,Simón C

    更新日期：2017-10-03 00:00:00

  • Cis- and trans-membrane interactions of synaptotagmin-1.

    abstract：:In neurotransmission synaptotagmin-1 tethers synaptic vesicles to the presynaptic plasma membrane by binding to acidic membrane lipids and SNAREs and promotes rapid SNARE-mediated fusion upon Ca(2+) triggering. However, recent studies suggested that upon membrane contact synaptotagmin may not only bind in trans to the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1116326109

    authors： Vennekate W,Schröder S,Lin CC,van den Bogaart G,Grunwald M,Jahn R,Walla PJ

    更新日期：2012-07-03 00:00:00

  • Parkinsonism-inducing neurotoxin, N-methyl-4-phenyl-1,2,3,6 -tetrahydropyridine: uptake of the metabolite N-methyl-4-phenylpyridine by dopamine neurons explains selective toxicity.

    abstract：:N-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) produces neuropathological and clinical abnormalities in humans, monkeys, and mice that closely resemble idiopathic parkinsonism. N-Methyl-4-phenylpyridine (MPP+), a metabolite of MPTP formed by monoamine oxidase B, is accumulated into striatal and cerebral cortical ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.7.2173

    authors： Javitch JA,D'Amato RJ,Strittmatter SM,Snyder SH

    更新日期：1985-04-01 00:00:00

  • Assignment of the human gene for galactose-1-phosphate uridyltransferase to chromosome 9: studies with Chinese hamster-human somatic cell hybrids.

    abstract：:Chinese hamster-human somatic cell hybrids were analyzed for the expression of human galactose-1-phosphate uridyltransferase (GALT; UDPglucose:alpha-D-galactose-1-phosphate uridyltransferase, EC 2.7.7.12) by electrophoresis and for the presence of human chromosomes cytogenetically with the aid of Q-banding. Three of t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.12.5628

    authors： Mohandas T,Sparkes RS,Sparkes MC,Shulkin JD

    更新日期：1977-12-01 00:00:00

  • Compositional landscape for glass formation in metal alloys.

    abstract：:A high-resolution compositional map of glass-forming ability (GFA) in the Ni-Cr-Nb-P-B system is experimentally determined along various compositional planes. GFA is shown to be a piecewise continuous function formed by intersecting compositional subsurfaces, each associated with a nucleation pathway for a specific cr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1407780111

    authors： Na JH,Demetriou MD,Floyd M,Hoff A,Garrett GR,Johnson WL

    更新日期：2014-06-24 00:00:00

  • The X-linked lymphoproliferative disease gene product SAP associates with PAK-interacting exchange factor and participates in T cell activation.

    abstract：:SLAM (signaling lymphocyte activation molecule)-associated protein (SAP) is a Src homology 2 (SH2) domain-containing adaptor expressed in T cells and natural killer cells. Its essential role in immune responses is underscored by the recent finding that mutations in SAP result in a rare but fatal X-linked lymphoprolife...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0606624103

    authors： Gu C,Tangye SG,Sun X,Luo Y,Lin Z,Wu J

    更新日期：2006-09-26 00:00:00

  • Monomeric and trimeric forms of photosystem I reaction center of Mastigocladus laminosus: crystallization and preliminary characterization.

    abstract：:Photosystem I (PSI) reaction centers (RCs) of the thermophilic cyanobacterium Mastigocladus laminosus were purified and characterized. The PSI RC was obtained in two forms, monomeric and trimeric. The two forms contained the same number of pigments per P700 and displayed similar photochemical activities. The two forms...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.12.5312

    authors： Almog O,Shoham G,Michaeli D,Nechushtai R

    更新日期：1991-06-15 00:00:00

  • Identification of an additional negative regulatory region for p53 sequence-specific DNA binding.

    abstract：:The DNA binding activity of p53 is crucial for its tumor suppressor function and is subject to tight regulation. Previous studies revealed that the inhibitory function of the p53 C terminus is implicated in the latent, low affinity sequence-specific DNA binding activity of p53 in the uninduced state. Sequence-specific...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.11.6079

    authors： Müller-Tiemann BF,Halazonetis TD,Elting JJ

    更新日期：1998-05-26 00:00:00

  • Galactose metabolic genes in yeast respond to a ratio of galactose and glucose.

    abstract：:Natural environments are filled with multiple, often competing, signals. In contrast, biological systems are often studied in "well-controlled" environments where only a single input is varied, potentially missing important interactions between signals. Catabolite repression of galactose by glucose is one of the best-...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1418058112

    authors： Escalante-Chong R,Savir Y,Carroll SM,Ingraham JB,Wang J,Marx CJ,Springer M

    更新日期：2015-02-03 00:00:00

  • Disruption of quercetin metabolism by fungicide affects energy production in honey bees (Apis mellifera).

    abstract：:Cytochrome P450 monooxygenases (P450) in the honey bee, Apis mellifera, detoxify phytochemicals in honey and pollen. The flavonol quercetin is found ubiquitously and abundantly in pollen and frequently at lower concentrations in honey. Worker jelly consumed during the first 3 d of larval development typically contains...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1614864114

    authors： Mao W,Schuler MA,Berenbaum MR

    更新日期：2017-03-07 00:00:00

  • A superior descriptor of random textures and its predictive capacity.

    abstract：:Two-phase random textures abound in a host of contexts, including porous and composite media, ecological structures, biological media, and astrophysical structures. Questions surrounding the spatial structure of such textures continue to pose many theoretical challenges. For example, can two-point correlation function...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0905919106

    authors： Jiao Y,Stillinger FH,Torquato S

    更新日期：2009-10-20 00:00:00

  • Protection of Cftr knockout mice from acute lung infection by a helper-dependent adenoviral vector expressing Cftr in airway epithelia.

    abstract：:We developed a helper-dependent adenoviral vector for cystic fibrosis lung gene therapy. The vector expresses cystic fibrosis transmembrane conductance regulator (Cftr) using control elements from cytokeratin 18. The vector expressed properly localized CFTR in cultured cells and in the airway epithelia of mice. Cftr R...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2436478100

    authors： Koehler DR,Sajjan U,Chow YH,Martin B,Kent G,Tanswell AK,McKerlie C,Forstner JF,Hu J

    更新日期：2003-12-23 00:00:00

  • Label-free probe of HIV-1 TAT peptide binding to mimetic membranes.

    abstract：:The transacting activator of transduction (TAT) protein plays a key role in the progression of AIDS. Studies have shown that a +8 charged sequence of amino acids in the protein, called the TAT peptide, enables the TAT protein to penetrate cell membranes. To probe mechanisms of binding and translocation of the TAT pept...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1411817111

    authors： Rao Y,Kwok SJ,Lombardi J,Turro NJ,Eisenthal KB

    更新日期：2014-09-02 00:00:00

  • Severity of ocean acidification following the end-Cretaceous asteroid impact.

    abstract：:Most paleo-episodes of ocean acidification (OA) were either too slow or too small to be instructive in predicting near-future impacts. The end-Cretaceous event (66 Mya) is intriguing in this regard, both because of its rapid onset and also because many pelagic calcifying species (including 100% of ammonites and more t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1418604112

    authors： Tyrrell T,Merico A,Armstrong McKay DI

    更新日期：2015-05-26 00:00:00

  • Kinetics of a Criegee intermediate that would survive high humidity and may oxidize atmospheric SO2.

    abstract：:Criegee intermediates are thought to play a role in atmospheric chemistry, in particular, the oxidation of SO2, which produces SO3 and subsequently H2SO4, an important constituent of aerosols and acid rain. However, the impact of such oxidation reactions is affected by the reactions of Criegee intermediates with water...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1513149112

    authors： Huang HL,Chao W,Lin JJ

    更新日期：2015-09-01 00:00:00

  • Chymotryptic specificity determinants in the 1.0 A structure of the zinc-inhibited human tissue kallikrein 7.

    abstract：:hK7 or human stratum corneum chymotryptic enzyme belongs to the human tissue kallikrein (hKs) serine proteinase family and is strongly expressed in the upper layers of the epidermis. It participates in skin desquamation but is also implicated in diverse skin diseases and is a potential biomarker of ovarian cancer. We ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0707811104

    authors： Debela M,Hess P,Magdolen V,Schechter NM,Steiner T,Huber R,Bode W,Goettig P

    更新日期：2007-10-09 00:00:00

  • Identification of specific apical membrane polypeptides associated with the antidiuretic hormone-elicited water permeability increase in the toad urinary bladder.

    abstract：:Antidiuretic hormone (ADH) increases the water permeability of the toad urinary bladder. The increase occurs in the apical plasma membrane of granular cells that line the urinary surface of the bladder and is produced by the insertion of water permeability units that have been identified by freeze-fracture electron mi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.6.1942

    authors： Harris HW Jr,Wade JB,Handler JS

    更新日期：1988-03-01 00:00:00

  • Resolution of a spectrum of nucleoprotein species in sonicated chromatin.

    abstract：:Sonicated rabbit-liver chromatin is fractionated by ion-exchange chromatography on ECTHAM-cellulose, a weakly cationic adsorbent. The chromatographic procedure provides a series of fractions having a spectrum of thermal denaturation profiles. The earliest-eluting fractions melt cooperatively at a significantly higher ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.8.2317

    authors： Reeck GR,Simpson RT,Sober HA

    更新日期：1972-08-01 00:00:00

  • First look at changes in flood hazard in the Inter-Sectoral Impact Model Intercomparison Project ensemble.

    abstract：:Climate change due to anthropogenic greenhouse gas emissions is expected to increase the frequency and intensity of precipitation events, which is likely to affect the probability of flooding into the future. In this paper we use river flow simulations from nine global hydrology and land surface models to explore unce...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1302078110

    authors： Dankers R,Arnell NW,Clark DB,Falloon PD,Fekete BM,Gosling SN,Heinke J,Kim H,Masaki Y,Satoh Y,Stacke T,Wada Y,Wisser D

    更新日期：2014-03-04 00:00:00

  • Scaling of number, size, and metabolic rate of cells with body size in mammals.

    abstract：:The size and metabolic rate of cells affect processes from the molecular to the organismal level. We present a quantitative, theoretical framework for studying relationships among cell volume, cellular metabolic rate, body size, and whole-organism metabolic rate that helps reveal the feedback between these levels of o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0611235104

    authors： Savage VM,Allen AP,Brown JH,Gillooly JF,Herman AB,Woodruff WH,West GB

    更新日期：2007-03-13 00:00:00

  • Methicillin-resistant Staphylococcus aureus in hospitals and the community: stealth dynamics and control catastrophes.

    abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) represents a serious threat to the health of hospitalized patients. Attempts to reduce the spread of MRSA have largely depended on hospital hygiene and patient isolation. These measures have met with mixed success: although some countries have almost eliminated MRSA o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0401324101

    authors： Cooper BS,Medley GF,Stone SP,Kibbler CC,Cookson BD,Roberts JA,Duckworth G,Lai R,Ebrahim S

    更新日期：2004-07-06 00:00:00

  • Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion.

    abstract：:Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0809575106

    authors： Martinez D,Challacombe J,Morgenstern I,Hibbett D,Schmoll M,Kubicek CP,Ferreira P,Ruiz-Duenas FJ,Martinez AT,Kersten P,Hammel KE,Vanden Wymelenberg A,Gaskell J,Lindquist E,Sabat G,Bondurant SS,Larrondo LF,Canessa P,Vic

    更新日期：2009-02-10 00:00:00

  • Messenger RNA for hepatic tryptophan oxygenase: its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones.

    abstract：:Messenger RNA from rat liver was partially purified by chromatography on cellulose on the basis of its poly(A) content. Microgram amounts of this RNA stimulate protein synthesis manyfold in a heterologous cell-free system, derived from Krebs ascites cells supplemented with reticulocyte initiation factors. The messenge...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.70.4.1218

    authors： Schutz G,Beato M,Feigelson P

    更新日期：1973-04-01 00:00:00

  • The Parkinson's disease protein DJ-1 is neuroprotective due to cysteine-sulfinic acid-driven mitochondrial localization.

    abstract：:Loss-of-function DJ-1 mutations can cause early-onset Parkinson's disease. The function of DJ-1 is unknown, but an acidic isoform accumulates after oxidative stress, leading to the suggestion that DJ-1 is protective under these conditions. We addressed whether this represents a posttranslational modification at cystei...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0402959101

    authors： Canet-Avilés RM,Wilson MA,Miller DW,Ahmad R,McLendon C,Bandyopadhyay S,Baptista MJ,Ringe D,Petsko GA,Cookson MR

    更新日期：2004-06-15 00:00:00

  • Lipoprotein lipase enhances the binding of chylomicrons to low density lipoprotein receptor-related protein.

    abstract：:Chylomicron catabolism is known to be initiated by the enzyme lipoprotein lipase (triacylglycero-protein acylhydrolase, EC 3.1.1.34). Chylomicron remnants, produced by lipolysis, are rapidly taken up by the liver via an apolipoprotein E (apoE)-mediated, receptor-dependent process. The low density lipoprotein (LDL) rec...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.19.8342

    authors： Beisiegel U,Weber W,Bengtsson-Olivecrona G

    更新日期：1991-10-01 00:00:00

  • Specific accumulation of tumor-derived adhesion factor in tumor blood vessels and in capillary tube-like structures of cultured vascular endothelial cells.

    abstract：:Tumor-derived adhesion factor (TAF) was previously identified as a cell adhesion molecule secreted by human bladder carcinoma cell line EJ-1. To elucidate the physiological function of TAF, we examined its distribution in human normal and tumor tissues. Immunochemical staining with an anti-TAF monoclonal antibody show...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.16.8384

    authors： Akaogi K,Okabe Y,Sato J,Nagashima Y,Yasumitsu H,Sugahara K,Miyazaki K

    更新日期：1996-08-06 00:00:00

  • Proenkephalin, [Met]enkephalin, and oxytocin immunoreactivities are colocalized in bovine hypothalamic magnocellular neurons.

    abstract：:The distribution of proenkephalin and [Met]enkephalin immunoreactivities in the bovine hypothalamo-neurohypophyseal system was studied by use of specific antisera. Proenkephalin and [Met]enkephalin immunoreactivities were found in magnocellular neuronal cell bodies in the dorsal part of the supraoptic nuclei and in th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.16.5139

    authors： Vanderhaeghen JJ,Lotstra F,Liston DR,Rossier J

    更新日期：1983-08-01 00:00:00

  • Barttin modulates trafficking and function of ClC-K channels.

    abstract：:Barttin is an accessory subunit of a subgroup of ClC-type chloride channels expressed in renal and inner ear epithelia. In this study, we examined the effects of barttin on two ClC-K channel isoforms, rat ClC-K1 and human ClC-Kb, using heterologous expression, patch clamping, confocal imaging, and flow cytometry. In t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0601631103

    authors： Scholl U,Hebeisen S,Janssen AG,Müller-Newen G,Alekov A,Fahlke C

    更新日期：2006-07-25 00:00:00

  • Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin.

    abstract：:A murine monoclonal antibody (OKT9) raised against human leukemic cells binds to a wide variety of leukemia and tumor cell lines and to a minority of leukemia cells taken directly from patients. Fetal thymus and liver are strongly reactive as are some normal, immature hemopoietic cells and activated lymphocytes. React...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.7.4515

    authors： Sutherland R,Delia D,Schneider C,Newman R,Kemshead J,Greaves M

    更新日期：1981-07-01 00:00:00