Glacial reduction and millennial-scale variations in Drake Passage throughflow.

abstract：:A glycoprotein, termed amphiregulin (AR), inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells. It has been purified to apparent homogeneity from serum-free conditioned medium of MCF-7 human breast carcinoma cells that had been trea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shoyab M,McDonald VL,Bradley JG,Todaro GJ

更新日期：1988-09-01 00:00:00

abstract：:A cDNA containing the full coding region of human cytochrome b5 was inserted into a vaccinia virus cDNA expression vector. Infection of human thymidine kinase-minus (TK-) 143 cells in culture with this recombinant virus resulted in production of 0.3 nmol of cytochrome b5 per mg of cell lysate protein. The expressed cy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aoyama T,Nagata K,Yamazoe Y,Kato R,Matsunaga E,Gelboin HV,Gonzalez FJ

更新日期：1990-07-01 00:00:00

abstract：:Cystic fibrosis (CF) is a recessive disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene. The most common symptoms include progressive lung disease and chronic digestive conditions. CF is the first human genetic disease to benefit from having five different species of animal models. De...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim K,Lane EA,Saftien A,Wang H,Xu Y,Wirtz-Peitz F,Perrimon N

更新日期：2020-05-12 00:00:00

abstract：:N-Acetylvalyl-tRNA1Val (AcVal-tRNA1Val) was bound to the P site of uniformly 32P-labeled 70S ribosomes from Escherichia coli and crosslinked to 16S RNA in the 30S ribosomal subunit by irradiation with light of 300-400 nm. To identify the crosslinked nucleotide in 16S RNA. AcVal-tRNA1Val-16S [32P]RNA was digested compl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prince JB,Taylor BH,Thurlow DL,Ofengand J,Zimmermann RA

更新日期：1982-09-01 00:00:00

abstract：:The free radicals nitric oxide and superoxide anion react to form peroxynitrite (ONOO-), a highly toxic oxidant species. In vivo formation of ONOO- has been demonstrated in shock and inflammation. Herein we provide evidence that cytotoxicity in cells exposed to ONOO- is mediated by DNA strand breakage and the subseque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szabó C,Zingarelli B,O'Connor M,Salzman AL

更新日期：1996-03-05 00:00:00

abstract：:Aphids maintain mutualistic symbioses involving consortia of coinherited organisms. All possess a primary endosymbiont, Buchnera, which compensates for dietary deficiencies; many also contain secondary symbionts, such as Hamiltonella defensa, which confers defense against natural enemies. Genome sequences of uncultiva...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moran NA,Degnan PH,Santos SR,Dunbar HE,Ochman H

更新日期：2005-11-22 00:00:00

abstract：:The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a C-terminal hydrophilic domain that is homologous to IIA protein(s) domains of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). C-terminal truncation mutants were constructed and expressed in Escherichia coli and their properties we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poolman B,Knol J,Mollet B,Nieuwenhuis B,Sulter G

更新日期：1995-01-31 00:00:00

abstract：:Cellular wound healing or the repair of plasma membrane/cell wall damage (plasma membrane damage) occurs frequently in nature. Although various cellular perturbations, such as DNA damage, spindle misalignment, and impaired daughter cell formation, are monitored by cell cycle checkpoint mechanisms in budding yeast, whe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kono K,Al-Zain A,Schroeder L,Nakanishi M,Ikui AE

更新日期：2016-06-21 00:00:00

abstract：:Little is known about stem cell biology or the specialized environments or niches believed to control stem cell renewal and differentiation in self-renewing tissues of the body. Functional assays for stem cells are available only for hematopoiesis and spermatogenesis, and the microenvironment, or niche, for hematopoie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shinohara T,Orwig KE,Avarbock MR,Brinster RL

更新日期：2001-05-22 00:00:00

abstract：:DNA triple helices offer new perspectives toward oligonucleotide-directed gene regulation. However, the poor stability of some of these structures might limit their use under physiological conditions. Specific ligands can intercalate into DNA triple helices and stabilize them. Molecular modeling and thermal denaturati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Escudé C,Nguyen CH,Kukreti S,Janin Y,Sun JS,Bisagni E,Garestier T,Hélène C

更新日期：1998-03-31 00:00:00

abstract：:In this study, we compared the transport of newly synthesized cholesterol with that of influenza virus hemagglutinin (HA) from the endoplasmic reticulum to the plasma membrane. The arrival of cholesterol on the cell surface was monitored by cyclodextrin removal, and HA transport was monitored by surface trypsinization...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heino S,Lusa S,Somerharju P,Ehnholm C,Olkkonen VM,Ikonen E

更新日期：2000-07-18 00:00:00

abstract：:Antimicrobial peptides are postulated to disrupt microbial phospholipid membranes. The prevailing molecular model is based on the formation of stable or transient pores although the direct observation of the fundamental processes is lacking. By combining rational peptide design with topographical (atomic force microsc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rakowska PD,Jiang H,Ray S,Pyne A,Lamarre B,Carr M,Judge PJ,Ravi J,Gerling UI,Koksch B,Martyna GJ,Hoogenboom BW,Watts A,Crain J,Grovenor CR,Ryadnov MG

更新日期：2013-05-28 00:00:00

abstract：:A recently described organ culture system for human thymocytes is shown to support the generation of a diverse T-cell receptor repertoire in vitro: thymocytes of the alpha beta lineage, including representatives of the V beta families 5.2/5.3, 6.7, and 8, accounted for the majority of T-cell receptor-positive cells th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Merkenschlager M,Fisher AG

更新日期：1992-05-15 00:00:00

abstract：:An inversion loop seen in heteroduplex mapping of the DNA of mature Mu phage induced from a lysogen is observed also in defective lambda phage carrying one end of Mu. 14% of the DNA of Mu, including this region, designated the G loop, is shown to be to the right of all known genes in the prophage map. The inhomogeneou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daniell E,Boram W,Abelson J

更新日期：1973-07-01 00:00:00

abstract：:The pore domain of voltage-gated potassium (Kv) channels consists of transmembrane helices S5 and S6, the turret, the pore helix, the selectivity filter, and the loop preceding S6, with a tertiary reentrant structure between S5 and S6. Using biogenic intermediates, mass tagging (pegylation), and a molecular tape measu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gajewski C,Dagcan A,Roux B,Deutsch C

更新日期：2011-02-22 00:00:00

abstract：:During goal-directed movements, primates are able to rapidly and accurately control an online trajectory despite substantial delay times incurred in the sensorimotor control loop. To address the problem of large delays, it has been proposed that the brain uses an internal forward model of the arm to estimate current a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mulliken GH,Musallam S,Andersen RA

更新日期：2008-06-17 00:00:00

abstract：:The reversible phosphorylation of tyrosine residues is an important mechanism for modulating biological processes such as cellular signaling, differentiation, and growth, and if deregulated, can result in various types of cancer. Therefore, an understanding of these dynamic cellular processes at the molecular level re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salomon AR,Ficarro SB,Brill LM,Brinker A,Phung QT,Ericson C,Sauer K,Brock A,Horn DM,Schultz PG,Peters EC

更新日期：2003-01-21 00:00:00

abstract：:The Arabidopsis thaliana SOS1 protein is a putative Na+/H+ antiporter that functions in Na+ extrusion and is essential for the NaCl tolerance of plants. sos1 mutant plants share phenotypic similarities with mutants lacking the protein kinase SOS2 and the Ca2+ sensor SOS3. To investigate whether the three SOS proteins ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quintero FJ,Ohta M,Shi H,Zhu JK,Pardo JM

更新日期：2002-06-25 00:00:00

abstract：:Wnts regulate important intracellular signaling events, and dysregulation of the Wnt pathway has been linked to human disease. Here, we uncover numerous Wnt canonical effectors in human platelets where Wnts, their receptors, and downstream signaling components have not been previously described. We demonstrate that th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Steele BM,Harper MT,Macaulay IC,Morrell CN,Perez-Tamayo A,Foy M,Habas R,Poole AW,Fitzgerald DJ,Maguire PB

更新日期：2009-11-24 00:00:00

abstract：:It recently has been shown that epithelial Na(+) channels are controlled by a receptor for intracellular Na(+), a G protein (G(o)), and a ubiquitin-protein ligase (Nedd4). Furthermore, mutations in the epithelial Na(+) channel that underlie the autosomal dominant form of hypertension known as Liddle's syndrome inhibit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishibashi H,Dinudom A,Harvey KF,Kumar S,Young JA,Cook DI

更新日期：1999-08-17 00:00:00

abstract：:Ultrastructural cytochemistry of natural killer cells enriched by Percoll gradient centrifugation showed them to possess arylsulfatase (aryl-sulfate sulfohydrolase, EC 3.1.6.1). The enzyme was located in vesicles, granules, and the parallel tubular arrays, organelles characteristic for cytotoxic lymphocytes. Biochemic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zucker-Franklin D,Grusky G,Yang JS

更新日期：1983-11-01 00:00:00

abstract：:A protein factor that participates in the formation of a covalent complex between the 80,000-dalton precursor of the adenovirus (Ad) terminal protein (pTP) and 5'-dCMP has been isolated and characterized. This 47,000-dalton protein, isolated from nuclear extracts of uninfected HeLa cells, has been designated nuclear f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nagata K,Guggenheimer RA,Enomoto T,Lichy JH,Hurwitz J

更新日期：1982-11-01 00:00:00

abstract：:Renal cell carcinoma (RCC) and normal kidney tissues have been examined from 34 patients with sporadic, nonhereditary RCC. Eighteen of the 21 cytogenetically examined tumors (86%) had a detectable anomaly of chromosome arm 3p distal to band 3p11.2-p13, manifested as a deletion, combined with the nonreciprocal transloc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kovacs G,Erlandsson R,Boldog F,Ingvarsson S,Müller-Brechlin R,Klein G,Sümegi J

更新日期：1988-03-01 00:00:00

abstract：:Observations made during the last ten years with the Chandra X-ray Observatory have shed much light on the cooling gas in the centers of clusters of galaxies and the role of active galactic nucleus (AGN) heating. Cooling of the hot intracluster medium in cluster centers can feed the supermassive black holes found in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blanton EL,Clarke TE,Sarazin CL,Randall SW,McNamara BR

更新日期：2010-04-20 00:00:00

abstract：:More than 21 million prescriptions for warfarin are written yearly in the U.S. Despite its importance, warfarin's target, vitamin K epoxide reductase (VKOR), has resisted purification since its identification in 1972. Here, we report its purification and reconstitution. HPC4, a calcium-specific antibody that recognize...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chu PH,Huang TY,Williams J,Stafford DW

更新日期：2006-12-19 00:00:00

abstract：:Understanding protein-protein interactions is central to our understanding of almost all complex biological processes. Computational tools exploiting rapidly growing genomic databases to characterize protein-protein interactions are urgently needed. Such methods should connect multiple scales from evolutionary conserv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gueudré T,Baldassi C,Zamparo M,Weigt M,Pagnani A

更新日期：2016-10-25 00:00:00

abstract：:In many eukaryotic systems during immune responses, mitogen-activated protein kinases (MAPKs) link cytoplasmic signaling to chromatin events by targeting transcription factors, chromatin remodeling complexes, and the RNA polymerase machinery. So far, knowledge on these events is scarce in plants and no attempts have b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rayapuram N,Jarad M,Alhoraibi HM,Bigeard J,Abulfaraj AA,Völz R,Mariappan KG,Almeida-Trapp M,Schlöffel M,Lastrucci E,Bonhomme L,Gust AA,Mithöfer A,Arold ST,Pflieger D,Hirt H

更新日期：2021-01-19 00:00:00

abstract：:The Madden-Julian oscillation (MJO) is the dominant mode of variability in the tropical atmosphere on intraseasonal timescales and planetary spatial scales. Despite the primary importance of the MJO and the decades of research progress since its original discovery, a generally accepted theory for its essential mechani...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Majda AJ,Stechmann SN

更新日期：2009-05-26 00:00:00

abstract：:RNA of a common strain of tobacco mosaic virus was sequentially reconstituted first with protein of the same strain and then with protein of a tomato strain. The reconstituted particles were treated with specific antibodies against the two strain particles and were examined electron microscopically to locate the regio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Otsuki Y,Takebe I,Ohno T,Fukuda M,Okada Y

更新日期：1977-05-01 00:00:00

abstract：:Efficient apoptotic cell clearance and induction of immunologic tolerance is a critical mechanism preventing autoimmunity and associated pathology. Our laboratory has reported that apoptotic cells induce tolerance by a mechanism dependent on the tryptophan catabolizing enzyme indoleamine 2,3 dioxygenase 1 (IDO1) in sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ravishankar B,Liu H,Shinde R,Chaudhary K,Xiao W,Bradley J,Koritzinsky M,Madaio MP,McGaha TL

更新日期：2015-08-25 00:00:00