Nucleoporin MOS7/Nup88 is required for mitosis in gametogenesis and seed development in Arabidopsis.

Abstract:

:Angiosperm reproduction is characterized by alternate diploid sporophytic and haploid gametophytic generations. Gametogenesis shares similarities with that of animals except for the formation of the gametophyte, whereby haploid cells undergo several rounds of postmeiotic mitosis to form gametes and the accessory cells required for successful reproduction. The mechanisms regulating gametophyte development in angiosperms are incompletely understood. Here, we show that the nucleoporin Nup88-homolog MOS7 (Modifier of Snc1,7) plays a crucial role in mitosis during both male and female gametophyte formation in Arabidopsis thaliana. Using a mutagenesis screen, we identify the mos7-5 mutant allele, which causes ovule and pollen abortion in MOS7/mos7-5 heterozygous plants, and preglobular stage embryonic lethality in homozygous mos7-5 seeds. During interphase, we show that MOS7 is localized to the nuclear membrane but, like many nucleoporins, is associated with the spindle apparatus during mitosis. We detect interactions between MOS7 and several nucleoporins known to control spindle dynamics, and find that in pollen from MOS7/mos7-5 heterozygotes, abortion is accompanied by a failure of spindle formation, cell fate specification, and phragmoplast activity. Most intriguingly, we show that following gamete formation by MOS7/mos7-5 heterozygous spores, inheritance of either the MOS7 or the mos7-5 allele by a given gamete does not correlate with its respective survival or abortion. Instead, we suggest a model whereby MOS7, which is highly expressed in the Pollen- and Megaspore Mother Cells, enacts a dosage-limiting effect on the gametes to enable their progression through subsequent mitoses.

authors

Park GT,Frost JM,Park JS,Kim TH,Lee JS,Oh SA,Twell D,Brooks JS,Fischer RL,Choi Y

doi

10.1073/pnas.1421911112

subject

Has Abstract

pub_date

2014-12-23 00:00:00

pages

18393-8

issue

51

eissn

0027-8424

issn

1091-6490

pii

1421911112

journal_volume

111

pub_type

杂志文章
  • Arabidopsis systemic immunity uses conserved defense signaling pathways and is mediated by jasmonates.

    abstract::In the absence of adaptive immunity displayed by animals, plants respond locally to biotic challenge via inducible basal defense networks activated through recognition and response to conserved pathogen-associated molecular patterns. In addition, immunity can be induced in tissues remote from infection sites by system...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0605423104

    authors: Truman W,Bennett MH,Kubigsteltig I,Turnbull C,Grant M

    更新日期:2007-01-16 00:00:00

  • WNK4 regulates activity of the epithelial Na+ channel in vitro and in vivo.

    abstract::Homeostasis of intravascular volume, Na(+), Cl(-), and K(+) is interdependent and determined by the coordinated activities of structurally diverse mediators in the distal nephron and the distal colon. The behavior of these flux pathways is regulated by the renin-angiotensin-aldosterone system; however, the mechanisms ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0611727104

    authors: Ring AM,Cheng SX,Leng Q,Kahle KT,Rinehart J,Lalioti MD,Volkman HM,Wilson FH,Hebert SC,Lifton RP

    更新日期:2007-03-06 00:00:00

  • Induction of wild-type p53 activity in human cancer cells by ribozymes that repair mutant p53 transcripts.

    abstract::Several groups have attempted to develop gene therapy strategies to treat cancer via introduction of the wild-type (wt) p53 cDNA into cancer cells. Unfortunately, these approaches do not result in regulated expression of the p53 gene and do not reduce expression of the mutant p53 that is overexpressed in cancerous cel...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.150104097

    authors: Watanabe T,Sullenger BA

    更新日期:2000-07-18 00:00:00

  • Complete amino acid sequence of variable domains from two monoclonal human anti-gamma globulins of the Wa cross-idiotypic group: suggestion that the J segments are involved in the structural correlate of the idiotype.

    abstract::The complete amino acid sequences of the variable regions of two human IgM anti-gamma globulins are reported. The two proteins, Sie and Wol, share idiotypic determinants that are distinct from those shared by the two previously sequenced IgM anti-gamma globulins, Lay and Pom [Capra, J. D. & Kehoe, J. M. (1974) Proc. N...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.6.3799

    authors: Andrews DW,Capra JD

    更新日期:1981-06-01 00:00:00

  • A better Amazon road network for people and the environment.

    abstract::The rapidly expanding network of roads into the Amazon is permanently altering the world's largest tropical forest. Most proposed road projects lack rigorous impact assessments or even basic economic justification. This study analyzes the expected environmental, social and economic impacts of 75 road projects, totalin...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1910853117

    authors: Vilela T,Malky Harb A,Bruner A,Laísa da Silva Arruda V,Ribeiro V,Auxiliadora Costa Alencar A,Julissa Escobedo Grandez A,Rojas A,Laina A,Botero R

    更新日期:2020-03-31 00:00:00

  • Mimicking the folding pathway to improve homology-free protein structure prediction.

    abstract::Since the demonstration that the sequence of a protein encodes its structure, the prediction of structure from sequence remains an outstanding problem that impacts numerous scientific disciplines, including many genome projects. By iteratively fixing secondary structure assignments of residues during Monte Carlo simul...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0811363106

    authors: DeBartolo J,Colubri A,Jha AK,Fitzgerald JE,Freed KF,Sosnick TR

    更新日期:2009-03-10 00:00:00

  • Evidence from central Mexico supporting the Younger Dryas extraterrestrial impact hypothesis.

    abstract::We report the discovery in Lake Cuitzeo in central Mexico of a black, carbon-rich, lacustrine layer, containing nanodiamonds, microspherules, and other unusual materials that date to the early Younger Dryas and are interpreted to result from an extraterrestrial impact. These proxies were found in a 27-m-long core as p...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 历史文章,杂志文章

    doi:10.1073/pnas.1110614109

    authors: Israde-Alcántara I,Bischoff JL,Domínguez-Vázquez G,Li HC,DeCarli PS,Bunch TE,Wittke JH,Weaver JC,Firestone RB,West A,Kennett JP,Mercer C,Xie S,Richman EK,Kinzie CR,Wolbach WS

    更新日期:2012-03-27 00:00:00

  • Structural diversity in social contagion.

    abstract::The concept of contagion has steadily expanded from its original grounding in epidemic disease to describe a vast array of processes that spread across networks, notably social phenomena such as fads, political opinions, the adoption of new technologies, and financial decisions. Traditional models of social contagion ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1116502109

    authors: Ugander J,Backstrom L,Marlow C,Kleinberg J

    更新日期:2012-04-17 00:00:00

  • Analogs of palmitoyl-CoA that are substrates for myristoyl-CoA:protein N-myristoyltransferase.

    abstract::Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase (Nmt1p; EC 2.3.1.97) is an essential enzyme that is highly selective for myristoyl-CoA in vivo. It is unclear why myristate (C14:0), a rare cellular fatty acid, has been selected for this covalent protein modification over more abundant fatty acids ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.21.10507

    authors: Rudnick DA,Lu T,Jackson-Machelski E,Hernandez JC,Li Q,Gokel GW,Gordon JI

    更新日期:1992-11-01 00:00:00

  • Bleaching in reef corals: Physiological and stable isotopic responses.

    abstract::During the late summer to fall of 1987, Caribbean reef corals experienced an intense and widespread discoloration event described as bleaching. Contrary to initial predictions, most bleached corals did not die. However, energy input from zooxanthellae decreased, as estimated from: (i) delta(13)C values, a measure of t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.23.9342

    authors: Porter JW,Fitt WK,Spero HJ,Rogers CS,White MW

    更新日期:1989-12-01 00:00:00

  • Modeling environmentally mediated rotavirus transmission: The role of temperature and hydrologic factors.

    abstract::Rotavirus is considered a directly transmitted disease due to its high infectivity. Environmental pathways have, therefore, largely been ignored. Rotavirus, however, persists in water sources, and both its surface water concentrations and infection incidence vary with temperature. Here, we examine the potential for wa...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章,meta分析

    doi:10.1073/pnas.1719579115

    authors: Kraay ANM,Brouwer AF,Lin N,Collender PA,Remais JV,Eisenberg JNS

    更新日期:2018-03-20 00:00:00

  • Effects of combined glutamine and serum deprivation on glucose control of hexose transport in mammalian fibroblast cultures.

    abstract::Regulation of hexose transport in NIL hamster fibroblasts has been studied in confluent cultures preconditioned for 24 hr in media deprived of glutamine or of serum or of both. Cultures maintained in media containing dialyzed fetal calf serum and 4 mM glutamine accumulated up to 72 nmol of glutamine per mg of cell pro...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.10.5958

    authors: Kalckar HM,Ullrey DB,Laursen RA

    更新日期:1980-10-01 00:00:00

  • Effect of phenotypic variation on kin selection.

    abstract::An expression for the equilibrium of the mean phenotypic value of a quantitative character is derived for a model in which the fitness of an individual depends on its own phenotype and the mean phenotypic value of a group of related individuals. When selection is weak the equilibrium mean is well predicted by Hamilton...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.12.7506

    authors: Boyd R,Richerson PJ

    更新日期:1980-12-01 00:00:00

  • DNA polymerase from temperate phage Bam35 is endowed with processive polymerization and abasic sites translesion synthesis capacity.

    abstract::DNA polymerases (DNAPs) responsible for genome replication are highly faithful enzymes that nonetheless cannot deal with damaged DNA. In contrast, translesion synthesis (TLS) DNAPs are suitable for replicating modified template bases, although resulting in very low-fidelity products. Here we report the biochemical cha...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1510280112

    authors: Berjón-Otero M,Villar L,de Vega M,Salas M,Redrejo-Rodríguez M

    更新日期:2015-07-07 00:00:00

  • UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.

    abstract::Enveloped viruses exploit the endomembrane system to enter host cells. Through a cascade of membrane-trafficking events, virus-bearing vesicles fuse with acidic endosomes and/or lysosomes mediated by SNAREs triggering viral fusion. However, the molecular mechanisms underlying this process remain elusive. Here, we foun...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1320629111

    authors: Pirooz SD,He S,Zhang T,Zhang X,Zhao Z,Oh S,O'Connell D,Khalilzadeh P,Amini-Bavil-Olyaee S,Farzan M,Liang C

    更新日期:2014-02-18 00:00:00

  • Inhibition of DNA synthesis by an electrophilic metabolite of benzo[a]pyrene.

    abstract::Mitogen-stimulated scheduled DNA synthesis and DNA excision repair in human lymphocytes, as well as DNA polymerase a activity in a cell-free system, were inhibited by an electrophilic metabolite of benzo[a]pyrene. This metabolite, (+/-)-anti-(7r,8t)-dihydroxy-(9,10t)-epoxy-7,8,9,10-tetrahyd robenzo[a]pyrene (BPDE), co...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.81.17.5300

    authors: Busbee DL,Joe CO,Norman JO,Rankin PW

    更新日期:1984-09-01 00:00:00

  • Hydrodynamic narrowing of tubes extruded from cells.

    abstract::We discuss the pulling force f required to extrude a lipid tube from a living cell as a function of the extrusion velocity L. The main feature is membrane friction on the cytoskeleton. As recently observed for neutrophils, the tether force exhibits a "shear thinning" response over a large range of pulling velocities, ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0602012103

    authors: Brochard-Wyart F,Borghi N,Cuvelier D,Nassoy P

    更新日期:2006-05-16 00:00:00

  • Alterations in cellular metabolism triggered by URA7 or GLN3 inactivation cause imbalanced dNTP pools and increased mutagenesis.

    abstract::Eukaryotic DNA replication fidelity relies on the concerted action of DNA polymerase nucleotide selectivity, proofreading activity, and DNA mismatch repair (MMR). Nucleotide selectivity and proofreading are affected by the balance and concentration of deoxyribonucleotide (dNTP) pools, which are strictly regulated by r...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1618714114

    authors: Schmidt TT,Reyes G,Gries K,Ceylan CÜ,Sharma S,Meurer M,Knop M,Chabes A,Hombauer H

    更新日期:2017-05-30 00:00:00

  • Risperidone and ritanserin but not haloperidol block effect of dizocilpine on the active allothetic place avoidance task.

    abstract::Spatial working memory or short-term place memory is impaired in schizophrenia. The efficiency of antipsychotic drugs, particularly of typical antipsychotics, on cognitive deficit in schizophrenia remains disputable. Inhibition of serotonin (5-HT) 2A/2C receptors is important for cognitive improvement in schizophrenic...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0711273105

    authors: Bubenikova-Valesova V,Stuchlik A,Svoboda J,Bures J,Vales K

    更新日期:2008-01-22 00:00:00

  • Mitogen-activated protein kinase pathways defend against bacterial pore-forming toxins.

    abstract::Cytolytic pore-forming toxins are important for the virulence of many disease-causing bacteria. How target cells molecularly respond to these toxins and whether or not they can mount a defense are poorly understood. By using microarrays, we demonstrate that the nematode Caenorhabditis elegans responds robustly to Cry5...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0404073101

    authors: Huffman DL,Abrami L,Sasik R,Corbeil J,van der Goot FG,Aroian RV

    更新日期:2004-07-27 00:00:00

  • Histone chaperone Anp32e removes H2A.Z from DNA double-strand breaks and promotes nucleosome reorganization and DNA repair.

    abstract::The repair of DNA double-strand breaks (DSBs) requires open, flexible chromatin domains. The NuA4-Tip60 complex creates these flexible chromatin structures by exchanging histone H2A.Z onto nucleosomes and promoting acetylation of histone H4. Here, we demonstrate that the accumulation of H2A.Z on nucleosomes at DSBs is...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1504868112

    authors: Gursoy-Yuzugullu O,Ayrapetov MK,Price BD

    更新日期:2015-06-16 00:00:00

  • Eye opening and PSD95 are required for long-term potentiation in developing superior colliculus.

    abstract::The only major glutamate receptor membrane-associated guanylate kinase scaffolds expressed in the young superficial superior colliculus (SC) are synapse-associated protein 102 (SAP102) and postsynaptic density protein 95 (PSD95). In this, as in all visual brain regions examined, synaptic PSD95 increases rapidly follow...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1215854110

    authors: Zhao JP,Murata Y,Constantine-Paton M

    更新日期:2013-01-08 00:00:00

  • Different mutation signatures in DNA polymerase eta- and MSH6-deficient mice suggest separate roles in antibody diversification.

    abstract::Hypermutation in immunoglobulin genes produces a high frequency of substitutions of all four bases, which are likely generated by low-fidelity DNA polymerases. Indeed, humans deficient for DNA polymerase (pol) eta have decreased substitutions of A.T base pairs in variable and switch regions. To study the role of pol e...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0501852102

    authors: Martomo SA,Yang WW,Wersto RP,Ohkumo T,Kondo Y,Yokoi M,Masutani C,Hanaoka F,Gearhart PJ

    更新日期:2005-06-14 00:00:00

  • Homologous DNA recombination in vertebrate cells.

    abstract::The RAD52 epistasis group genes are involved in homologous DNA recombination, and their primary structures are conserved from yeast to humans. Although biochemical studies have suggested that the fundamental mechanism of homologous DNA recombination is conserved from yeast to mammals, recent studies of vertebrate cell...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章,评审

    doi:10.1073/pnas.111006398

    authors: Sonoda E,Takata M,Yamashita YM,Morrison C,Takeda S

    更新日期:2001-07-17 00:00:00

  • High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C.

    abstract::Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains r...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.24.12003

    authors: Velander WH,Johnson JL,Page RL,Russell CG,Subramanian A,Wilkins TD,Gwazdauskas FC,Pittius C,Drohan WN

    更新日期:1992-12-15 00:00:00

  • Photosynthetic carbon reduction pathway is absent in chloroplasts of Vicia faba guard cells.

    abstract::Four cell types from Vicia faba Linnaeus "Long Pod" leaflets were assayed for three enzymes unique to the photosynthetic carbon reduction pathway. The enzymes were ribulosebisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39], phosphoribulokinase (ATP:D-ribulose-5-phosphate 1-phospho...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.12.6371

    authors: Outlaw WH,Manchester J,Dicamelli CA,Randall DD,Rapp B,Veith GM

    更新日期:1979-12-01 00:00:00

  • The 28-kDa calbindin-D is a major calcium-binding protein in the basilar papilla of the chick.

    abstract::In previous work we identified a basilar papilla protein (BPP23) that appears to be one of the most abundant soluble proteins in the basilar papilla of the chick cochlea. Here we report the purification of protein BPP23 from chick cochlea and the generation of a specific antiserum. Immunoblotting and immunoprecipitati...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.10.3387

    authors: Oberholtzer JC,Buettger C,Summers MC,Matschinsky FM

    更新日期:1988-05-01 00:00:00

  • Biochemical stratagem for obligate parasitism of eukaryotic cells by Coxiella burnetii.

    abstract::Coxiella burnetti, the etiologic agent of Q fever, is an oligate intracellular parasite of eukaryotes. Unlike the majority of successful bacterial parasites, which escape the bactericidal environment of the phagolysosome by various means, C. burnetii multiplies only in the phagolysosome. In view of the relatively hars...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.5.3240

    authors: Hackstadt T,Williams JC

    更新日期:1981-05-01 00:00:00

  • Resistance to apoptosis caused by PIG-A gene mutations in paroxysmal nocturnal hemoglobinuria.

    abstract::Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal hematopoietic stem cell disorder resulting from mutations in an X-linked gene, PIG-A, that encodes an enzyme required for the first step in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. PIG-A mutations result in absent or decreased cell surface ex...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.16.8756

    authors: Brodsky RA,Vala MS,Barber JP,Medof ME,Jones RJ

    更新日期:1997-08-05 00:00:00

  • Dramatic amplification of a rice transposable element during recent domestication.

    abstract::Despite the prevalence of transposable elements in the genomes of higher eukaryotes, what is virtually unknown is how they amplify to very high copy numbers without killing their host. Here, we report the discovery of rice strains where a miniature inverted-repeat transposable element (mPing) has amplified from approx...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0605421103

    authors: Naito K,Cho E,Yang G,Campbell MA,Yano K,Okumoto Y,Tanisaka T,Wessler SR

    更新日期:2006-11-21 00:00:00