Abstract：
:Stem cell-based therapy is a promising treatment for neurodegenerative diseases. In our laboratory, a novel protocol has been developed to induce bone marrow-derived mesenchymal stem cells (MSC) into neurotrophic factors- secreting cells (NTF-SC), thus combining stem cell-based therapy with the NTF-based neuroprotection. These cells produce and secrete factors such as brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor. Conditioned medium of the NTF-SC that was applied to a neuroblastoma cell line (SH-SY5Y) 1 h before exposure to the neurotoxin 6-hydroxydopamine (6-OHDA) demonstrated marked protection. An efficacy study was conducted on the 6-OHDA-induced lesion, a rat model of Parkinson's disease. The cells, either MSC or NTF-SC, were transplanted on the day of 6-OHDA administration and amphetamine-induced rotations were measured as a primary behavior index. We demonstrated that when transplanted posterior to the 6-OHDA lesion, the NTF-SC ameliorated amphetamine-induced rotations by 45%. HPLC analysis demonstrated that 6-OHDA induced dopamine depletion to a level of 21% compared to the untreated striatum. NTF-SC inhibited dopamine depletion to a level of 72% of the contralateral striatum. Moreover, an MRI study conducted with iron-labeled cells, followed by histological verification, revealed that the engrafted cells migrated toward the lesion. In a histological assessment, we found that the cells induced regeneration in the damaged striatal dopaminergic nerve terminal network. We therefore conclude that the induced MSC have a therapeutic potential for neurodegenerative processes and diseases, both by the NTFs secretion and by the migratory trait toward the diseased tissue.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Sadan O,Bahat-Stromza M,Barhum Y,Levy YS,Pisnevsky A,Peretz H,Ilan AB,Bulvik S,Shemesh N,Krepel D,Cohen Y,Melamed E,Offen Ddoi
10.1089/scd.2008.0411subject
Has Abstractpub_date
2009-10-01 00:00:00pages
1179-90issue
8eissn
1547-3287issn
1557-8534journal_volume
18pub_type
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journal_title：Stem cells and development
pub_type： 杂志文章,评审
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abstract：:Traumatic spinal cord injury (SCI) leads to a cascade of secondary chemical insults, including oxidative stress and glutamate excitotoxicity, which damage host neurons and glia. Transplantation of exogenous neural stem/progenitor cells (NSPCs) has shown promise in enhancing regeneration after SCI, although survival of...
journal_title：Stem cells and development
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journal_title：Stem cells and development
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journal_title：Stem cells and development
pub_type： 杂志文章
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2008.0237
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0239
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2017.0048
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abstract：:Expression of the transcription factor Snail is required for normal vasculogenesis in the developing mouse embryo. In addition, tumors expressing Snail have been associated with a more malignant phenotype, with both increased invasive properties and angiogenesis. Although the relationship between Snail and vasculogene...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0194
更新日期：2012-01-20 00:00:00
abstract：:Bone marrow human mesenchymal stem cells (hMSCs) are known to survive in serum-free media, when most normal somatic cells do not survive. We found that the endogenously-activated bone morphogenetic protein (BMP) pathway is involved in this cellular behavior. Under this culture condition, phosphorylated Smad1 (PSmad1),...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0020
更新日期：2009-11-01 00:00:00
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0234
更新日期：2013-04-15 00:00:00
abstract：:Bronchopulmonary dysplasia (BPD), the main complication of extreme prematurity, has lifelong consequences for lung health. Mesenchymal stromal cells (MSCs) prevent lung injury in experimental BPD in newborn rodents when given in the immediate neonatal period. Whether MSC therapy can restore normal lung growth after es...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2019.0116
更新日期：2020-03-15 00:00:00
abstract：:Human embryonic stem cells (hESCs) have the potential to differentiate into many adult cell types, and they are being explored as a resource for cell replacement therapies for multiple diseases. In order to optimize in vitro differentiation protocols, it will be necessary to elucidate regulatory mechanisms that contri...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0224
更新日期：2010-06-01 00:00:00
abstract：:Mouse Embryonic Stem Cells (mESCs) are unique in their self-renewal and pluripotency. Hypothetically, mESCs model gestational stress effects or stresses of in vitro fertilization/assisted reproductive technologies or drug/environmental exposures that endanger embryos. Testing mESCs stress responses should diminish and...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2018.0157
更新日期：2019-01-15 00:00:00
abstract：:The mammalian inner ear is vulnerable to genetic disorders and aging, as well as to injuries caused by overstimulation, ototoxic drugs, and viral infections. Due to the poor regeneration of the sensory epithelium and the spiral ganglion neurons in the adult mammalian inner ear, cell replacement therapy strategies have...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2006.15.449
更新日期：2006-06-01 00:00:00
abstract：:Although the pluripotent and proliferative capacity of embryonic germ (EG) cells is thought to be equivalent to that of embryonic stem (ES) cells, there has been far less attention focused on the potential use of EG cells for applications in developing novel strategies of tissue transplantation in the treatment of deg...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2008.0383
更新日期：2010-02-01 00:00:00
abstract：:Allogeneic stem cell (SC)-based therapy is a promising tool for the treatment of a range of human degenerative and inflammatory diseases. Many reports highlighted the immune modulatory properties of some SC types, such as mesenchymal stromal cells (MSCs), but a comparative study with SCs of different origin, to assess...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0204
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abstract：:Recent studies have focused on transcriptional regulation and gene expression profiling of human embryonic stem cells (hESCs). However, little information is available regarding the relationship between RNA expression and transcriptional regulation, which is critical in the complete understanding of pluripotency and d...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2006.15.315
更新日期：2006-06-01 00:00:00
abstract：:In this retrospective study, 209 patients with malignant disease were analyzed for levels of T-cell receptor excision circles (TRECs) for the first 24 months after allogeneic stem cell transplantation. CD3(+) cells were separated by direct antibody-coupled magnetic beads, followed by DNA extraction according to a stan...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0588
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2020.0133
更新日期：2020-12-01 00:00:00
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2006.15.575
更新日期：2006-08-01 00:00:00
abstract：:Differential staining is an immunocytochemical staining that visualizes trophectoderm (TE) and the inner cell mass (ICM) of the blastocysts. It is used to determine the blastocyst quality, but could also be a useful tool to assess the integration site of injected cells into the early embryo. This is relevant for testi...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0503
更新日期：2015-05-01 00:00:00
abstract：:Human perivascular progenitor cells, including pericytes, are well-described multipotent mesenchymal cells giving rise to mesenchymal stem cells in culture. Despite the unique location of pericytes, specific antigens to distinguish human pericytes from other cell types are few. Here, we employed a human tissue microar...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2019.0106
更新日期：2019-09-15 00:00:00
abstract：:Vascular smooth muscle cells (SMCs), which arise from multiple embryonic progenitors, have unique lineage-specific properties and this diversity may contribute to spatial patterns of vascular diseases. We developed in vitro methods to generate distinct vascular SMC subtypes from human pluripotent stem cells, allowing ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0520
更新日期：2015-04-01 00:00:00
abstract：:The aim of this study was to clarify the mechanisms that regulate hematopoietic cell expansion in vitro by identifying defined culture conditions. We report the results of experiments with CD34(+) cells from cord blood (CB, n = 13), bone marrow (BM, n = 4), and mobilized peripheral blood stem cells (PBSC, n = 5) using...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2004.13.362
更新日期：2004-08-01 00:00:00
abstract：:We determined whether cartilage could be engineered from mesenchymal progenitor cells (MPCs) normally found in amniotic fluid. Mesenchymal amniocytes were isolated from ovine amniotic fluid samples (n = 5) and had their identity confirmed by immunocytochemistry. Cells were expanded and then cultured as micromass pelle...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2006.15.245
更新日期：2006-04-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) have a superior immunomodulatory capacity compared to other cells of the immune system, and they hold great promise for treating various immune disorders. However, their regulatory effects on the maturation of immature dendritic cells (imDCs) are not fully understood. In this study, we sh...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2017.0235
更新日期：2018-04-15 00:00:00
