Dual regulation of NR2B and NR2C expression by NMDA receptor activation in mouse cerebellar granule cell cultures.

abstract：:Whether the regulatory protein of an inducible operon for a catabolic pathway is a positive or a negative element in the control of expression is correlated with the frequent or infrequent presence, respectively, of the system's substrate in the natural environment. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Savageau MA

更新日期：1974-06-01 00:00:00

abstract：:Previous work has shown that serotonin induces an increase in membrane K+ conductance in Aplysia neuron R15 and that this response is mediated by cAMP. The present study examines the role of protein phosphorylation in the response to serotonin. A specific inhibitor of cAMP-dependent protein kinase was injected intrace...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adams WB,Levitan IB

更新日期：1982-06-01 00:00:00

abstract：:In enteric bacteria, the cellular response to oxidative stress is activated by oxidation of the iron-sulfur clusters in SoxR, which then induces transcription of soxS, turning on a battery of defense genes. Here we demonstrate both in vitro and in cells that activation of SoxR can occur in a DNA-mediated reaction with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee PE,Demple B,Barton JK

更新日期：2009-08-11 00:00:00

abstract：:We have analyzed the level of intraindividual sequence variability (heteroplasmy) of mtDNA in human brain by denaturing gradient gel electrophoresis and sequencing. Single base substitutions, as well as insertions or deletions of single bases, were numerous in the noncoding control region (D-loop), and 35-45% of the m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jazin EE,Cavelier L,Eriksson I,Oreland L,Gyllensten U

更新日期：1996-10-29 00:00:00

abstract：:Fibroblasts cultured from the skin of a patient with metachromatic leukodystrophy have been found to manifest the biochemical defect of this inborn error of metabolism, a deficiency of arylsulfatase A. Diseased cells had less than five per cent of normal arylsulfatase-A activity, while activities of other lysosomal en...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Porter MT,Fluharty AL,Kihara H

更新日期：1969-03-01 00:00:00

abstract：:A model of protein-ligand binding kinetics, in which slow solvent dynamics results from hydrophobic drying transitions, is investigated. Molecular dynamics simulations show that solvent in the receptor pocket can fluctuate between wet and dry states with lifetimes in each state that are long enough for the extraction ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mondal J,Morrone JA,Berne BJ

更新日期：2013-08-13 00:00:00

abstract：:Access to robust and information-rich human cardiac tissue models would accelerate drug-based strategies for treating heart disease. Despite significant effort, the generation of high-fidelity adult-like human cardiac tissue analogs remains challenging. We used computational modeling of tissue contraction and assembly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thavandiran N,Dubois N,Mikryukov A,Massé S,Beca B,Simmons CA,Deshpande VS,McGarry JP,Chen CS,Nanthakumar K,Keller GM,Radisic M,Zandstra PW

更新日期：2013-12-03 00:00:00

abstract：:Under fasting conditions, increases in circulating glucagon maintain glucose balance by promoting hepatic gluconeogenesis. Triggering of the cAMP pathway stimulates gluconeogenic gene expression through the PKA-mediated phosphorylation of the cAMP response element binding (CREB) protein and via the dephosphorylation o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai WW,Niessen S,Goebel N,Yates JR 3rd,Guccione E,Montminy M

更新日期：2013-05-28 00:00:00

abstract：:Chromosome segregation and mitotic exit are initiated by the 1.2-MDa ubiquitin ligase APC/C (anaphase-promoting complex/cyclosome) and its coactivator CDC20 (cell division cycle 20). To avoid chromosome missegregation, APC/C(CDC20) activation is tightly controlled. CDC20 only associates with APC/C in mitosis when APC/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qiao R,Weissmann F,Yamaguchi M,Brown NG,VanderLinden R,Imre R,Jarvis MA,Brunner MR,Davidson IF,Litos G,Haselbach D,Mechtler K,Stark H,Schulman BA,Peters JM

更新日期：2016-05-10 00:00:00

abstract：:Stimulation of the phosphatidylinositol cycle by neurotransmitters generates diacylglycerol, an activator of protein kinase C, which may regulate some forms of neurotransmission. Phorbol esters, potent inflammatory and tumor-promoting compounds, also activate protein kinase C. We demonstrate potent and selective effec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baraban JM,Gould RJ,Peroutka SJ,Snyder SH

更新日期：1985-01-01 00:00:00

abstract：:The mitochondrial genome of the S-type male-sterile cytoplasm of maize contains two linear episomes, S1 (6397 base pairs) and S2 (5453 base pairs). The S2 episome contains two large unidentified open reading frames, URF1 (3512 base pairs) and URF2 (1017 base pairs). We have demonstrated that a polypeptide with an appa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zabala G,O'brien-Vedder C,Walbot V

更新日期：1987-11-01 00:00:00

abstract：:Both polarities of the satellite RNA of tobacco ringspot virus are sources of self-cleaving sequences. RNA of the less abundant, negative polarity, designated sTobRV-(-)RNA, has cleaving activity that was mapped previously to two noncontiguous regions of the polyribonucleotide chain. Endoribonucleolytic oligoribonucle...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldstein PA,Buzayan JM,van Tol H,deBear J,Gough GR,Gilham PT,Bruening G

更新日期：1990-04-01 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) are involved in a wide range of regulatory processes in the cell. Owing to their flexibility, their conformations are expected to be particularly sensitive to the crowded cellular environment. Here we use single-molecule Förster resonance energy transfer to quantify the effect ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soranno A,Koenig I,Borgia MB,Hofmann H,Zosel F,Nettels D,Schuler B

更新日期：2014-04-01 00:00:00

abstract：:Invading organisms may spread through local movements (giving rise to a diffusion-like process) and by long-distance jumps, which are often human-mediated. The local spread of invading organisms has been fit with varying success to models that couple local population growth with diffusive spread, but to date no quanti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suarez AV,Holway DA,Case TJ

更新日期：2001-01-30 00:00:00

abstract：:Photoactivation of the retinal photoreceptor rhodopsin proceeds through a cascade of intermediates, resulting in protein-protein interactions catalyzing the activation of the G-protein transducin (Gt). Using stabilization and photoregeneration of the receptor's signaling state and Gt activation assays, we provide evid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kisselev OG,Meyer CK,Heck M,Ernst OP,Hofmann KP

更新日期：1999-04-27 00:00:00

abstract：:ATP-sensitive potassium channels (K(ATP)) are involved in a diverse array of physiologic functions including protection of tissue against ischemic insult, regulation of vascular tone, and modulation of insulin secretion. To improve our understanding of the role of K(ATP) in these processes, we used a gene-targeting st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chutkow WA,Samuel V,Hansen PA,Pu J,Valdivia CR,Makielski JC,Burant CF

更新日期：2001-09-25 00:00:00

abstract：:The polypeptide precursor pr76 to the internal viral group specific (gs) antigen proteins of Rous sarcoma virus, synthesized in a cell-free system of ascites cells, has been processed in vitro into the viral proteins by purified viral protein p15 as well as by disrupted Rous sarcoma virus. Disrupted Rauscher murine le...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： von der Helm K

更新日期：1977-03-01 00:00:00

abstract：:In this paper, we report the transcriptional organization of a 450-kb gene cluster in Xq28, flanked by the glucose-6-phosphate dehydrogenase and the color vision genes. CpG islands previously identified and mapped to distal Xq28 have helped in construction of a continuous contig of cosmids and in identification of cDN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bione S,Tamanini F,Maestrini E,Tribioli C,Poustka A,Torri G,Rivella S,Toniolo D

更新日期：1993-12-01 00:00:00

abstract：:Serotonin (5-hydroxytryptamine; 0.5 microM and above) stimulated the synthesis of prostacyclin (as measured by radioimmunoassay of 6-ketoprostaglandin F1 alpha) by bovine aortic smooth muscle cells in culture. This effect was structurally specific; a similar response was not elicited by the other indoles (tryptophan, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coughlin SR,Moskowitz MA,Antoniades HN,Levine L

更新日期：1981-11-01 00:00:00

abstract：:Protective immunity against human schistosome infection develops slowly, for reasons that are not yet fully understood. For many decades, researchers have attempted to infer properties of the immune response from epidemiological studies, with mathematical models frequently being used to bridge the gap between immunolo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell KM,Mutapi F,Savill NJ,Woolhouse ME

更新日期：2012-08-14 00:00:00

abstract：:Cancer therapies using genes and other macromolecules might realize their full clinical potential if they could be delivered to tumor tissue in optimal quantities. Unfortunately, the compromised circulation within tumors poses a formidable resistance to adequate and uniform penetration of these agents. Previously, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Netti PA,Hamberg LM,Babich JW,Kierstead D,Graham W,Hunter GJ,Wolf GL,Fischman A,Boucher Y,Jain RK

更新日期：1999-03-16 00:00:00

abstract：:A form of two-dimensional (2D) vibrational spectroscopy, which uses two ultrafast IR laser pulses, is used to examine the structure of a cyclic penta-peptide in solution. Spectrally resolved cross peaks occur in the off-diagonal region of the 2D IR spectrum of the amide I region, analogous to those in 2D NMR spectrosc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hamm P,Lim M,DeGrado WF,Hochstrasser RM

更新日期：1999-03-02 00:00:00

abstract：:Coacervation is a common phenomenon in natural polymers and has been applied to synthetic materials systems for coatings, adhesives, and encapsulants. Single-component coacervates are formed when block polyampholytes exhibit self-coacervation, phase separating into a dense liquid coacervate phase rich in the polyampho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Danielsen SPO,McCarty J,Shea JE,Delaney KT,Fredrickson GH

更新日期：2019-04-23 00:00:00

abstract：:Transcripts from mitochondrial (maxicircle) genes of kinetoplastid organisms undergo RNA editing characterized by a series of reactions that insert and delete uridine nucleotides within the sequence of the pre-mRNAs. Guide RNAs, which complement fully edited mRNAs, provide the information for the edited sequence by an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Göringer HU,Koslowsky DJ,Morales TH,Stuart K

更新日期：1994-03-01 00:00:00

abstract：:A procedure is described for selection of temperature-sensitive mutants affecting fatty-acid synthesis based upon radiation suicide of wild-type organisms by tritiated acetate selectively incorporated into fatty acids. At 37 degrees , two of the mutants extensively incorporate fatty-acid supplements provided in the me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harder ME,Beacham IR,Cronan JE Jr,Beacham K,Honegger JL,Silbert DF

更新日期：1972-11-01 00:00:00

abstract：:Island biogeography theory posits that species richness increases with island size and decreases with isolation. This logic underpins much conservation policy and regulation, with preference given to conserving large, highly connected areas, and relative ambivalence shown toward protecting small, isolated habitat patc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wintle BA,Kujala H,Whitehead A,Cameron A,Veloz S,Kukkala A,Moilanen A,Gordon A,Lentini PE,Cadenhead NCR,Bekessy SA

更新日期：2019-01-15 00:00:00

abstract：:The flux of solvent water coupled to the transit of ions through protein pores is considerable. The effect of this electroosmotic solvent flow on the binding of a neutral molecule [beta-cyclodextrin (betaCD)] to sites within the staphylococcal alpha-hemolysin pore was investigated. Mutant alpha-hemolysin pores were us...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gu LQ,Cheley S,Bayley H

更新日期：2003-12-23 00:00:00

abstract：:Nicotinic AChRs (nAChRs) represent a paradigm for ligand-gated ion channels. Despite intensive studies over many years, our understanding of the mechanisms of activation and inhibition for nAChRs is still incomplete. Here, we present molecular dynamics (MD) simulations of the alpha7 nAChR ligand-binding domain, both i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yi M,Tjong H,Zhou HX

更新日期：2008-06-17 00:00:00

abstract：:We performed the biochemical and biophysical characterization of a red fluorescent protein, eqFP611, from the sea anemone Entacmaea quadricolor cloned in Escherichia coli. With an excitation maximum at 559 nm and an emission maximum at 611 nm, the recombinant protein shows the most red-shifted emission and the largest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiedenmann J,Schenk A,Röcker C,Girod A,Spindler KD,Nienhaus GU

更新日期：2002-09-03 00:00:00

abstract：:Acyl-homoserine lactone (acyl-HSL) signaling is thought to mediate quorum sensing in many species of Proteobacteria. The opportunistic human pathogen Pseudomonas aeruginosa uses acyl-HSLs to regulate hundreds of genes, including many that code for extracellular virulence factors. The idea that the P. aeruginosa acyl-H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yarwood JM,Volper EM,Greenberg EP

更新日期：2005-06-21 00:00:00