Temperature-sensitive mutants of Escherichia coli requiring saturated and unsaturated fatty acids for growth: isolation and properties.

abstract：:Recently it has become apparent that N = 2 supersymmetric quantum field theory has something to do with cluster algebras. I review one aspect of the connection: supersymmetric quantum field theories have associated hyperkähler moduli spaces, and these moduli spaces carry a structure that looks like an extension of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neitzke A

更新日期：2014-07-08 00:00:00

abstract：:In free-viewing experiments, primates orient preferentially toward faces and face-like stimuli. To investigate the neural basis of this behavior, we measured the spontaneous viewing preferences of monkeys with selective bilateral amygdala lesions. The results revealed that when faces and nonface objects were presented...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taubert J,Flessert M,Wardle SG,Basile BM,Murphy AP,Murray EA,Ungerleider LG

更新日期：2018-07-31 00:00:00

abstract：:A monoclonal antibody was produced to human peripheral blood T cells. This hybridoma antibody, termed OKT4, was reactive by indirect immunofluorescence with only 55-60% of the peripheral blood T cell population (OKT4+) and unreactive with normal B cells, null cells, and macrophages. The OKT4- T cell population contain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reinherz EL,Kung PC,Goldstein G,Schlossman SF

更新日期：1979-08-01 00:00:00

abstract：:To study C(0)a priori estimates for solutions to certain complex Monge-Ampère equations, I introduce a coherent sheaf of ideals and show that it satisfies various global algebrogeometric conditions, including a cohomology vanishing theorem. This technique is used to establish the existence of Kähler-Einstein metrics o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nadel AM

更新日期：1989-10-01 00:00:00

abstract：:Positional cloning has already produced the sequences of more than 70 human genes associated with specific diseases. In addition to their medical importance, these genes are of interest as a set of human genes isolated solely on the basis of the phenotypic effect of the respective mutations. We analyzed the protein se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mushegian AR,Bassett DE Jr,Boguski MS,Bork P,Koonin EV

更新日期：1997-05-27 00:00:00

abstract：:For brain functions such as working memory and motor planning, neuronal circuits are able to sustain persistent activity after transient inputs. Theoretical studies have suggested that persistent activity can exist in recurrently connected networks as active reverberation. However, the actual cellular processes underl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lau PM,Bi GQ

更新日期：2005-07-19 00:00:00

abstract：:During cellular stresses, phosphorylation of eukaryotic initiation factor-2 (eIF2) elicits gene expression designed to ameliorate the underlying cellular disturbance. Central to this stress response is the transcriptional regulator activating transcription factor, ATF4. Here we describe the mechanism regulating ATF4 e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vattem KM,Wek RC

更新日期：2004-08-03 00:00:00

abstract：:Basophils are powerful mediators of Th2 immunity and are present in increased numbers during allergic inflammation and helminth infection. Despite their ability to potentiate Th2 immunity the mechanisms regulating basophil development remain largely unknown. We have found a unique role for isotype-switched antibodies ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herbst T,Esser J,Prati M,Kulagin M,Stettler R,Zaiss MM,Hewitson JP,Merky P,Verbeek JS,Bourquin C,Camberis M,Prout M,Maizels RM,Le Gros G,Harris NL

更新日期：2012-09-11 00:00:00

abstract：:Allostery, the coupling between ligand binding and protein conformational change, is the heart of biological network and it has often been explained by two representative models, the induced-fit and the population-shift models. Here, we clarified for what systems one model fits better than the other by performing mole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okazaki K,Takada S

更新日期：2008-08-12 00:00:00

abstract：:In vitro translation of Rous sarcoma virus (RSV) virion RNA in the messenger-dependent reticulocyte lysate system yielded polypeptides that were not synthesized by translation of RNA from a transformation-defective deletion mutant of RSV. These RSV-specific products migrated on sodium dodecyl sulfate/polyacrylamide ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beemon K,Hunter T

更新日期：1977-08-01 00:00:00

abstract：:Despite the ubiquitous use of phosphoramidite chemistry in the synthesis of biophosphates, catalytic asymmetric phosphoramidite transfer remains largely unexplored for phosphate ester synthesis. We have discovered that a tetrazole-functionalized peptide, in the presence of 10-Å molecular sieves, functions as an enanti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jordan PA,Kayser-Bricker KJ,Miller SJ

更新日期：2010-11-30 00:00:00

abstract：:Telomerase is an RNA-directed DNA polymerase, composed of RNA and protein subunits, that replicates the telomere ends of linear eukaryotic chromosomes. Using a genetic strategy described here, we identify the product of the EST2 gene, Est2p, as a subunit of telomerase in the yeast Saccharomyces cerevisiae. Est2p is re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Counter CM,Meyerson M,Eaton EN,Weinberg RA

更新日期：1997-08-19 00:00:00

abstract：:Real-world studies show that the facial expressions produced during pain and orgasm-two different and intense affective experiences-are virtually indistinguishable. However, this finding is counterintuitive, because facial expressions are widely considered to be a powerful tool for social interaction. Consequently, de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen C,Crivelli C,Garrod OGB,Schyns PG,Fernández-Dols JM,Jack RE

更新日期：2018-10-23 00:00:00

abstract：:The discipline-wide effort to database the fossil record at the occurrence level has made it possible to estimate marine invertebrate extinction and origination rates with much greater accuracy. The new data show that two biotic mechanisms have hastened recoveries from mass extinctions and confined diversity to a rela...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alroy J

更新日期：2008-08-12 00:00:00

abstract：:Treatment of human fibroblast cells with human fibroblast (beta)interferon for up to 8 hr resulted in the accumulation of at least four mRNAs. The mRNAs were isolated from cellular polysomes and characterized by stimulation of translation in a wheat germ cell-free protein synthesis system. The mRNAs appear as early as...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colonno RJ

更新日期：1981-08-01 00:00:00

abstract：:Transcription is an intrinsically dynamic process and requires the coordinated interplay of RNA polymerases (RNAPs) with nucleic acids and transcription factors. Classical structural biology techniques have revealed detailed snapshots of a subset of conformational states of the RNAP as they exist in crystals. A detail...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz S,Gietl A,Smollett K,Tinnefeld P,Werner F,Grohmann D

更新日期：2016-03-29 00:00:00

abstract：:The incidence of pediatric adrenal cortical carcinoma (ACC) in southern Brazil is 10-15 times higher than that of pediatric ACC worldwide. Because childhood ACC is associated with Li-Fraumeni syndrome, we examined the cancer history and p53 status of 36 Brazilian patients and their families. Remarkably, 35 of 36 patie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ribeiro RC,Sandrini F,Figueiredo B,Zambetti GP,Michalkiewicz E,Lafferty AR,DeLacerda L,Rabin M,Cadwell C,Sampaio G,Cat I,Stratakis CA,Sandrini R

更新日期：2001-07-31 00:00:00

abstract：:Rhizobium meliloti strain SU47 produces the calcofluor-binding exopolysaccharide, succinoglycan, that is required for alfalfa root nodule invasion. Strains derived from R. meliloti SU47 secreted an acidic exopolysaccharide, EPSb, that replaced succinoglycan in nodule invasion. EPSb, which has not formerly been identif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhan HJ,Levery SB,Lee CC,Leigh JA

更新日期：1989-05-01 00:00:00

abstract：:We provide here evidence that supports the occurrence of a biologically dormant form of selectin ligand carbohydrate, the sialyl 6-sulfo Lewis X containing modified sialic acid, in human leukocytes. The modification of sialic acid involves first de-N-acetylation of sialic acid moiety through ubiquitous de-N-acetylatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitsuoka C,Ohmori K,Kimura N,Kanamori A,Komba S,Ishida H,Kiso M,Kannagi R

更新日期：1999-02-16 00:00:00

abstract：:The helical ribosome structures of Mycoplasma gallisepticum have been analyzed by optical diffraction and the rotational symmetry technique. The helix repeat (510 A) is composed of 10 ribosomes in 3 turns. The rotational studies show some ribosome fine structure. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maniloff J

更新日期：1971-01-01 00:00:00

abstract：:Relatively low concentrations of methylxanthines increase spontaneous twitching in cultured muscle fibers; they also cause a second activity, escalation, which is the initiation and undiminished propagation of visible waves. The effect on twitching is abolished by depolarizing concentrations of K(+) or by tetrodotoxin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coleman AW,Coleman JR,Griffin JD,Weltman JK,Chapman KM

更新日期：1972-03-01 00:00:00

abstract：:Phyllosphere microbial communities were evaluated on leaves of field-grown plant species by culture-dependent and -independent methods. Denaturing gradient gel electrophoresis (DGGE) with 16S rDNA primers generally indicated that microbial community structures were similar on different individuals of the same plant sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang CH,Crowley DE,Borneman J,Keen NT

更新日期：2001-03-27 00:00:00

abstract：:For disordered proteins, the dimensions of the chain are an important property that is sensitive to environmental conditions. We have used single-molecule Förster resonance energy transfer to probe the temperature-induced chain collapse of five unfolded or intrinsically disordered proteins. Because this behavior is se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wuttke R,Hofmann H,Nettels D,Borgia MB,Mittal J,Best RB,Schuler B

更新日期：2014-04-08 00:00:00

abstract：:Fine finger and hand movements in humans, monkeys, and rats are under the direct control of the corticospinal tract (CST). CST lesions lead to severe, long-term deficits of precision movements. We transected completely both CSTs in adult rats and treated the animals for 2 weeks with an antibody that neutralized the ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raineteau O,Fouad K,Noth P,Thallmair M,Schwab ME

更新日期：2001-06-05 00:00:00

abstract：:In mice, recent thymic emigrants (RTEs) make up a large part of the naïve T cell pool and have been suggested to be a distinct short-lived pool. In humans, however, the life span and number of RTEs are unknown. Although (2)H(2)O labeling in young mice showed high thymic-dependent daily naïve T cell production, long te...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vrisekoop N,den Braber I,de Boer AB,Ruiter AF,Ackermans MT,van der Crabben SN,Schrijver EH,Spierenburg G,Sauerwein HP,Hazenberg MD,de Boer RJ,Miedema F,Borghans JA,Tesselaar K

更新日期：2008-04-22 00:00:00

abstract：:Locomotion of the nematode Caenorhabditis elegans is a key observable used in investigations ranging from behavior to neuroscience to aging. However, while the natural environment of this model organism is 3D, quantitative investigations of its locomotion have been mostly limited to 2D motion. Here, we present a quant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bilbao A,Patel AK,Rahman M,Vanapalli SA,Blawzdziewicz J

更新日期：2018-04-17 00:00:00

abstract：:Recent analysis of complete eukaryotic genome sequences has revealed that gene duplication has been rampant. Moreover, next to a continuous mode of gene duplication, in many eukaryotic organisms the complete genome has been duplicated in their evolutionary past. Such large-scale gene duplication events have been assoc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maere S,De Bodt S,Raes J,Casneuf T,Van Montagu M,Kuiper M,Van de Peer Y

更新日期：2005-04-12 00:00:00

abstract：:Previous work identified the Rap1 GTPase-activating protein Sipa1 as a germ-line-encoded metastasis modifier. The bromodomain protein Brd4 physically interacts with and modulates the enzymatic activity of Sipa1. In vitro analysis of a highly metastatic mouse mammary tumor cell line ectopically expressing Brd4 demonstr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crawford NP,Alsarraj J,Lukes L,Walker RC,Officewala JS,Yang HH,Lee MP,Ozato K,Hunter KW

更新日期：2008-04-29 00:00:00

abstract：:After decades of robust growth, the rise in US life expectancy stalled after 2010. Explanations for the stall have focused on rising drug-related deaths. Here we show that a stagnating decline in cardiovascular disease (CVD) mortality was the main culprit, outpacing and overshadowing the effects of all other causes of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mehta NK,Abrams LR,Myrskylä M

更新日期：2020-03-31 00:00:00

abstract：:The positions of transition states along reaction coordinates (r++) for simple chemical reactions are often estimated from Leffler alpha values, the slope of plots of DeltaG++ (activation energy) versus DeltaG(0) (equilibrium free energy) for a series of structural variants. Protein folding is more complex than simple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fersht AR

更新日期：2004-10-05 00:00:00