Analysis of the helical ribosome structures of Mycoplasma gallisepticum.

abstract：:The redox potentials of electron transfer proteins vary over a wide range, even when the type of redox center is the same. Rees [Proc. Natl. Acad. Sci. USA (1985) 82, 3082-3085] proposed that this variation of redox potential partly reflects the different net charges of the proteins, and he presented a linear correlat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moore GR,Pettigrew GW,Rogers NK

更新日期：1986-07-01 00:00:00

abstract：:SgrS RNA is a model for the large class of Hfq-associated small RNAs that act to posttranscriptionally regulate bacterial mRNAs. The function of SgrS is well-characterized in nonpathogenic Escherichia coli, where it was originally shown to counteract glucose-phosphate stress by acting as a repressor of the ptsG mRNA, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papenfort K,Podkaminski D,Hinton JC,Vogel J

更新日期：2012-03-27 00:00:00

abstract：:The inference of the evolutionary history of a set of languages is a complex problem. Although some languages are known to be related through descent from common ancestral languages, for other languages determining whether such a relationship holds is itself a difficult problem. In this paper we report on new methods,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warnow T

更新日期：1997-06-24 00:00:00

abstract：:The nucleotide sequence of a 1,163-base-pair fragment that encodes the entire thyA gene of Escherichia coli K-12 was determined. The strategy involved sequence determination of both DNA strands by using overlapping deletions that had been generated in vitro from the two ends of the fragment with BAL-31 nuclease. The a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Belfort M,Maley G,Pedersen-Lane J,Maley F

更新日期：1983-08-01 00:00:00

abstract：:Angiogenin, a potent inducer of neovascularization in the chicken chorioallantoic membrane and rabbit cornea, promotes endothelial cell invasion of Matrigel basement membrane. A transformed bovine aortic endothelial cell line, GM 7373, is 5 times more invasive when cultured in the presence of 1 microgram of bovine ang...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu G,Riordan JF,Vallee BL

更新日期：1994-12-06 00:00:00

abstract：:The blue-light receptor genes (sopII) of sensory rhodopsin (SR) II were cloned from two species, the halophilic bacteria Haloarcula vallismortis (vSR-II) and Natronobacterium pharaonis (pSR-II). Upstream of both sopII gene loci, sequences corresponding to the halobacterial transducer of rhodopsin (Htr) II were recogni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seidel R,Scharf B,Gautel M,Kleine K,Oesterhelt D,Engelhard M

更新日期：1995-03-28 00:00:00

abstract：:The measurement of any nonchromosomal genetic contribution to the heritability of a trait is often confounded by the inability to control both the chromosomal and nonchromosomal information in a population. We have designed a unique system in yeast where we can control both sources of information so that the phenotype...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edwards MD,Symbor-Nagrabska A,Dollard L,Gifford DK,Fink GR

更新日期：2014-05-27 00:00:00

abstract：:The homogeneous exchange process whereby IgG molecules adsorbed onto latex particles are replaced by IgG molecules from the bulk solution was studied by means of 125I radiolabeling. The exchange mechanism was investigated on surfaces saturated with either labeled or unlabeled proteins in the presence of a solution of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ball V,Huetz P,Elaissari A,Cazenave JP,Voegel JC,Schaaf P

更新日期：1994-07-19 00:00:00

abstract：:Monospecific antibodies were produced in vitro by fusing mouse myeloma cells with spleen cells from a BALB/c mouse immunized with rat skeletal myofibrils. After cloning 3 times on agarose, two stable clones were obtained and chosen for further characterization. The first clone, JLB1, produced an antibody that recogniz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin JJ

更新日期：1981-04-01 00:00:00

abstract：:Acetyl-CoA carboxylase 1 (ACC1) catalyzes the formation of malonyl-CoA, the C2 donor for de novo synthesis of long-chain fatty acids. We have identified 64 exons, including 7 alternatively spliced minor exons (1A, 1B, 1C, 3, 5A', 5A, and 5B) in human ACC1 gene ( approximately 330 kb). The gene is regulated by three pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mao J,Chirala SS,Wakil SJ

更新日期：2003-06-24 00:00:00

abstract：:Based largely on studies in xenograft models, lipocalin-2 (Lcn2) has been implicated in the progression of multiple types of human tumors, including breast cancer. Here we examine the role of Lcn2 in mammary tumorigenesis and lung metastasis using an in vivo molecular genetics approach. We crossed a well-characterized...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berger T,Cheung CC,Elia AJ,Mak TW

更新日期：2010-02-16 00:00:00

abstract：:The Mecp2 gene has been shown to be mutated in most cases of human Rett syndrome, and mouse models deleted for the ortholog have been generated. Lineage-specific deletion of the gene indicated that the Rett-like phenotype is caused by Mecp2 deficiency in neurons. Biochemical evidence suggests that Mecp2 acts as a glob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tudor M,Akbarian S,Chen RZ,Jaenisch R

更新日期：2002-11-26 00:00:00

abstract：:Normal monkeys and monkeys with lesions of the hippocampal formation and adjacent cortex (the H+ lesion) were trained on the delayed nonmatching to sample (DNMS) task with a delay of 0.5 s between the sample and the choice. The animals with H+ lesions learned the task normally at this short delay and also exhibited th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alvarez P,Zola-Morgan S,Squire LR

更新日期：1994-06-07 00:00:00

abstract：:The phosphorylation of protein I, a specific neuronal protein, has been found to be regulated both by cyclic AMP (cAMP) and by calcium, in intact as well as in lysed synaptosome preparations from rat brain. In order to determine the phosphorylation site(s) of protein I that were regulated by cAMP and calcium, protein ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huttner WB,Greengard P

更新日期：1979-10-01 00:00:00

abstract：:The Ediacara biota include macroscopic, morphologically complex soft-bodied organisms that appear globally in the late Ediacaran Period (575-542 Ma). The physiology, feeding strategies, and functional morphology of the modular Ediacara organisms (rangeomorphs and erniettomorphs) remain debated but are critical for und...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laflamme M,Xiao S,Kowalewski M

更新日期：2009-08-25 00:00:00

abstract：:We used Monte Carlo methods to treat statistical problem of electrostatic interactions among many titrating amino acids and applied these methods to lysozyme and the photosynthetic reaction center of Rhodobacter sphaeroides, including all titrating sites. We computed the average protonation of residues as a function o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beroza P,Fredkin DR,Okamura MY,Feher G

更新日期：1991-07-01 00:00:00

abstract：:Previous work has shown that serotonin induces an increase in membrane K+ conductance in Aplysia neuron R15 and that this response is mediated by cAMP. The present study examines the role of protein phosphorylation in the response to serotonin. A specific inhibitor of cAMP-dependent protein kinase was injected intrace...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adams WB,Levitan IB

更新日期：1982-06-01 00:00:00

abstract：:Rational engineering methods can be applied with reasonable success to optimize physicochemical characteristics of proteins, in particular, antibodies. Here, we describe a combined CDR3 walking randomization and rational design-based approach to enhance the affinity of the human anti-gastrin TA4 scFv. The application ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barderas R,Desmet J,Timmerman P,Meloen R,Casal JI

更新日期：2008-07-01 00:00:00

abstract：:The postdevelopmental basis of cellular identity and the unique cellular output of a particular neuron type are of particular interest in the nervous system because a detailed understanding of circuits responsible for complex processes in the brain is impeded by the often ambiguous classification of neurons in these c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz DJ,Goaillard JM,Marder EE

更新日期：2007-08-07 00:00:00

abstract：:Despite the widespread use of silicon in modern technology, its peculiar thermal expansion is not well understood. Adapting harmonic phonons to the specific volume at temperature, the quasiharmonic approximation, has become accepted for simulating the thermal expansion, but has given ambiguous interpretations for micr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim DS,Hellman O,Herriman J,Smith HL,Lin JYY,Shulumba N,Niedziela JL,Li CW,Abernathy DL,Fultz B

更新日期：2018-02-27 00:00:00

abstract：:The maspin protein has tumor suppressor activity in breast and prostate cancers. It inhibits cell motility and invasion in vitro and tumor growth and metastasis in nude mice. Maspin is structurally a member of the serpin (serine protease inhibitors) superfamily but deviates somewhat from classical serpins. We find tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sheng S,Truong B,Fredrickson D,Wu R,Pardee AB,Sager R

更新日期：1998-01-20 00:00:00

abstract：:Genetic variants underlying life-threatening diseases, being unlikely to be transmitted to the next generation, are gradually and selectively eliminated from the population through negative selection. We study the determinants of this evolutionary process in human genes underlying monogenic diseases by comparing vario...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rapaport F,Boisson B,Gregor A,Béziat V,Boisson-Dupuis S,Bustamante J,Jouanguy E,Puel A,Rosain J,Zhang Q,Zhang SY,Gleeson JG,Quintana-Murci L,Casanova JL,Abel L,Patin E

更新日期：2021-01-19 00:00:00

abstract：:Metal-catalyzed oxidation results in loss of function and structural alteration of proteins. The oxidative process affects a variety of side amino acid groups, some of which are converted to carbonyl compounds. Spectrophotometric measurement of these moieties, after their reaction with 2,4-dinitrophenylhydrazine, is a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Requena JR,Chao CC,Levine RL,Stadtman ER

更新日期：2001-01-02 00:00:00

abstract：:Light chains were obtained from IgG rabbit antibodies to the group-specific carbohydrates of groups A and C streptococci. An analysis of the amino acid alternatives which exist at the first three positions of the N-terminus in both light-chain preparations shows a marked restriction in amino acid sequence heterogeneit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hood L,Lackland H,Eichman K,Kindt TJ,Braun DG,Krause RM

更新日期：1969-07-01 00:00:00

abstract：:Telomerase activity was identified in extracts from several different mouse cell lines. Addition of telomeric TTAGGG repeats was specific to telomeric oligonucleotide primers and sensitive to pretreatment with RNase A. In contrast to the hundreds of repeats synthesized by the human and Tetrahymena telomerase enzymes i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prowse KR,Avilion AA,Greider CW

更新日期：1993-02-15 00:00:00

abstract：:The high susceptibility of human immunoglobulin D to proteolytic degradation affects its biological function, metabolism, and immunoassay. High-pressure liquid chromatography was used to investigate the mechanism and rate of limited proteolytic cleavage of IgD and also to identify, isolate, and quantify the reaction p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishioka N,Takahashi N,Putnam FW

更新日期：1987-01-01 00:00:00

abstract：:Ca(2+) is essential for physiological depolarization-evoked synchronous neurotransmitter release. But, whether Ca(2+) influx or another factor controls release initiation is still under debate. The time course of ACh release is controlled by a presynaptic inhibitory G protein-coupled autoreceptor (GPCR), whose agonist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kupchik YM,Rashkovan G,Ohana L,Keren-Raifman T,Dascal N,Parnas H,Parnas I

更新日期：2008-03-18 00:00:00

abstract：:Photo-triggered alpha-helix formation of a 16-residue peptide featuring a built-in conformational photoswitch is monitored by time-resolved IR spectroscopy. An experimental approach with 2-ps time resolution and a scanning range up to 30 micros is used to cover all time scales of the peptide dynamics. Experiments are ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bredenbeck J,Helbing J,Kumita JR,Woolley GA,Hamm P

更新日期：2005-02-15 00:00:00

abstract：:Immunoprecipitation of proteins extracted from metabolically labeled human glioblastoma and fibrosarcoma cells with antiserum to platelet-derived growth factor (PDGF) showed that these cells express and secrete proteins that are recognized specifically by the antiserum. The molecular masses of immunoprecipitated prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pantazis P,Pelicci PG,Dalla-Favera R,Antoniades HN

更新日期：1985-04-01 00:00:00

abstract：:Historical data provide a baseline against which to judge the significance of recent ecological shifts and guide conservation strategies, especially for species decimated by pre-20th century harvesting. Northern fur seals (NFS; Callorhinus ursinus) are a common pinniped species in archaeological sites from southern Ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Newsome SD,Etnier MA,Gifford-Gonzalez D,Phillips DL,van Tuinen M,Hadly EA,Costa DP,Kennett DJ,Guilderson TP,Koch PL

更新日期：2007-06-05 00:00:00