Single-molecule spectroscopy reveals polymer effects of disordered proteins in crowded environments.

abstract：:A high molecular weight protein (HMWP) with properties similar to those of both actin-binding protein (ABP) and filamin has been isolated from cultured baby hamster kidney (BHK-21) cells. The protein was present in an actomyosin-depleted sucrose extract of the cells and was eluted, upon gel chromatography on Sepharose...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schloss JA,Goldman RD

更新日期：1979-09-01 00:00:00

abstract：:A major function of innate immune receptors is to recognize pathogen-associated molecular patterns and then evoke immune responses appropriate to the nature of the invading pathogen(s). Because innate immune cells express various types of these receptors, distinct combinations of signaling pathways are activated in re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Negishi H,Matsuki K,Endo N,Sarashina H,Miki S,Matsuda A,Fukazawa K,Taguchi-Atarashi N,Ikushima H,Yanai H,Nishio J,Honda K,Fujioka Y,Ohba Y,Noda T,Taniguchi S,Nishida E,Zhang Y,Chi H,Flavell RA,Taniguchi T

更新日期：2013-12-03 00:00:00

abstract：:Sex differences in parenting are common in species where both males and females provide care. Although there is a considerable body of game and optimality theory for why the sexes should differ in parental care, genetics can also play a role, and no study has examined how genetic influences might influence differences...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walling CA,Stamper CE,Smiseth PT,Moore AJ

更新日期：2008-11-25 00:00:00

abstract：:Dihydropteridine reductase (EC 1.6.99.7) is a member of the recently identified family of proteins known as short-chain dehydrogenases. When the x-ray structure of dihydropteridine reductase is correlated with conserved amino acid sequences characteristic of this enzyme class, two important common structural regions c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Varughese KI,Xuong NH,Kiefer PM,Matthews DA,Whiteley JM

更新日期：1994-06-07 00:00:00

abstract：:Sheep antibodies directed against an AMP-bovine serum albumin conjugate exhibit highly specific binding toward AMP. These antibodies bind to the AMP moiety of adenylylated glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] from Escherichia coli and to no other antigenic determinant on the prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hohman RJ,Rhee SG,Stadtman ER

更新日期：1980-12-01 00:00:00

abstract：:Incubation of physiological concentrations of 125I-labeled insulin with liver membranes in the presence of anti-insulin IgG results in a 7- to 15-fold increase in the specific binding of the hormone. The low-affinity/high-capacity binding sites are replaced by an apparently homogeneous class of high-affinity sites, an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shechter Y,Chang KJ,Jacobs S,Cuatrecasas P

更新日期：1979-06-01 00:00:00

abstract：:Concern for the suffering of others is central to moral decision making. How humans evaluate others' suffering, relative to their own suffering, is unknown. We investigated this question by inviting subjects to trade off profits for themselves against pain experienced either by themselves or an anonymous other person....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crockett MJ,Kurth-Nelson Z,Siegel JZ,Dayan P,Dolan RJ

更新日期：2014-12-02 00:00:00

abstract：:A powerful way to deal with a complex system is to build a coarse-grained model capable of catching its main physical features, while being computationally affordable. Inevitably, such coarse-grained models introduce a set of phenomenological parameters, which are often not easily deducible from the underlying atomist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Invernizzi M,Valsson O,Parrinello M

更新日期：2017-03-28 00:00:00

abstract：:Although the left and right human cerebral hemispheres differ both functionally and anatomically, little is known about the environmental or genetic factors that govern central nervous system asymmetry. Nevertheless, cerebral asymmetry is strongly correlated with handedness, and handedness does have a significant gene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geschwind DH,Miller BL,DeCarli C,Carmelli D

更新日期：2002-03-05 00:00:00

abstract：:The cytosolic concentration of free Ca2+ in bovine neutrophils was monitored by using the intracellular Ca2+ indicator quin2, 2-[[2-bis(acetylamino)-5-methylphenoxy]methyl-6-methoxy-8- bis(acetylamino)]quinoline. Neutrophils at rest have a cytosolic Ca2+ concentration of 85 +/- 5 nM, which in 2-4 min increases to 300-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gennaro R,Pozzan T,Romeo D

更新日期：1984-03-01 00:00:00

abstract：:Serotonin (5-HT) plays a key role in early brain development, and manipulation of 5-HT levels during this period can have lasting neurobiological and behavioral consequences. It is unclear how perinatal exposure to drugs, such as selective serotonin reuptake inhibitors (SSRIs), impacts cortical neural network function...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simpson KL,Weaver KJ,de Villers-Sidani E,Lu JY,Cai Z,Pang Y,Rodriguez-Porcel F,Paul IA,Merzenich M,Lin RC

更新日期：2011-11-08 00:00:00

abstract：:Perhydrohistrionicotoxin, at concentrations of 10(-12)-10(-7) M, depressed the current generated by iontophoretic application of acetylcholine to endplate regions of soleus and extensor digitorum longus muscles of rats. However, no changes in the amplitude or time course of spontaneous miniature endplate potentials or...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Albuquerque EX,Gage PW

更新日期：1978-03-01 00:00:00

abstract：:Autism spectrum disorder (ASD) is characterized by impaired social interactions and communication. The pathogenesis of ASD is not known, but it involves activation of microglia. We had shown that the peptide neurotensin (NT) is increased in the serum of children with ASD and stimulates cultured adult human microglia t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsilioni I,Pantazopoulos H,Conti P,Leeman SE,Theoharides TC

更新日期：2020-07-14 00:00:00

abstract：:The foundation of marine coral reef ecosystems is calcium carbonate accumulated primarily by the action of hard corals (Coelenterata: Anthozoa: Scleractinia). Colonial hard coral polyps cover the surface of the reef and deposit calcium carbonate as the aragonite polymorph, stabilized into a continuous calcareous skele...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Domart-Coulon IJ,Elbert DC,Scully EP,Calimlim PS,Ostrander GK

更新日期：2001-10-09 00:00:00

abstract：:Integrins contain two structurally homologous but distantly related domains: an I-like domain that is present in all beta-subunits and an I domain that is present in some alpha-subunits. Atomic resolution and mutagenesis studies of alpha I domains demonstrate a C-terminal, axial displacement of the alpha7-helix that a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang W,Shimaoka M,Chen J,Springer TA

更新日期：2004-02-24 00:00:00

abstract：:Expansion of polyglutamine repeats in several unrelated proteins causes neurodegenerative diseases with distinct but related pathologies. To provide a model system for investigating common pathogenic features, we have examined the behavior of polyglutamine expansions expressed in Caenorhabditis elegans. The expression...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Satyal SH,Schmidt E,Kitagawa K,Sondheimer N,Lindquist S,Kramer JM,Morimoto RI

更新日期：2000-05-23 00:00:00

abstract：:Fragment screens for new ligands have had wide success, notwithstanding their constraint to libraries of 1,000-10,000 molecules. Larger libraries would be addressable were molecular docking reliable for fragment screens, but this has not been widely accepted. To investigate docking's ability to prioritize fragments, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teotico DG,Babaoglu K,Rocklin GJ,Ferreira RS,Giannetti AM,Shoichet BK

更新日期：2009-05-05 00:00:00

abstract：:Members of the ras gene family are activated as oncogenes in many different human cancers. To systematically determine the frequency at which such genes might be involved in the neoplastic process affecting a specific target tissue, urothelial cells, we surveyed a large series of urinary tract tumors for ras oncogenes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fujita J,Srivastava SK,Kraus MH,Rhim JS,Tronick SR,Aaronson SA

更新日期：1985-06-01 00:00:00

abstract：:Complete sequences for the intergenic regions of the genome of human respiratory syncytial virus were obtained by dideoxynucleotide sequencing using synthetic oligonucleotides. These experiments established that the 10 respiratory syncytial viral genes are arranged, without additional intervening genes, in the order 3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins PL,Dickens LE,Buckler-White A,Olmsted RA,Spriggs MK,Camargo E,Coelingh KV

更新日期：1986-07-01 00:00:00

abstract：:Viruses are fundamental components of marine microbial communities that significantly influence oceanic productivity, biogeochemistry, and ecosystem processes. Despite their importance, the temporal activities and dynamics of viral assemblages in natural settings remain largely unexplored. Here we report the transcrip...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aylward FO,Boeuf D,Mende DR,Wood-Charlson EM,Vislova A,Eppley JM,Romano AE,DeLong EF

更新日期：2017-10-24 00:00:00

abstract：:Graphene superlattices were shown to exhibit high-temperature quantum oscillations due to periodic emergence of delocalized Bloch states in high magnetic fields such that unit fractions of the flux quantum pierce a superlattice unit cell. Under these conditions, semiclassical electron trajectories become straight agai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krishna Kumar R,Mishchenko A,Chen X,Pezzini S,Auton GH,Ponomarenko LA,Zeitler U,Eaves L,Fal'ko VI,Geim AK

更新日期：2018-05-15 00:00:00

abstract：:The synaptic vesicle membrane protein synaptotagmin (tagmin) is essential for fast, calcium-dependent, neurotransmitter release and is likely to be the calcium sensor for exocytosis, because of its many calcium-dependent properties. Polyphosphoinositides are needed for exocytosis, but it has not been known why. We now...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schiavo G,Gu QM,Prestwich GD,Söllner TH,Rothman JE

更新日期：1996-11-12 00:00:00

abstract：:The spindle checkpoint ensures the accuracy of chromosome segregation during mitosis. The protein serine/threonine kinase, Mps1, is a critical component of the spindle checkpoint in human cells and regulates the kinetochore localization of key checkpoint proteins. The kinase activity of Mps1 is required for the spindl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang J,Chen Y,Zhao Y,Yu H

更新日期：2007-12-18 00:00:00

abstract：:The crystal structure of the dodecanucleotide d(CGCAAGCTGGCG) has been determined to a resolution of 2.5 A and refined to an R factor of 19.3% for 1710 reflections. The sequence crystallizes as a B-type double helix, with two G(anti).A(syn) base pairs. These are stabilized by three-center hydrogen bonds to pyrimidines...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Webster GD,Sanderson MR,Skelly JV,Neidle S,Swann PF,Li BF,Tickle IJ

更新日期：1990-09-01 00:00:00

abstract：:A low molecular weight toxin isolated from the venom of the digger wasp Philanthus triangulum, first noted by T. Piek, is a potent antagonist of transmission at quisqualate-sensitive glutamate synapses of locust leg muscle. This philanthotoxin 433 (PTX-433) has been purified, chemically characterized, and subsequently...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eldefrawi AT,Eldefrawi ME,Konno K,Mansour NA,Nakanishi K,Oltz E,Usherwood PN

更新日期：1988-07-01 00:00:00

abstract：:The family of WD40-repeat (WDR) proteins is one of the largest in eukaryotes, but little is known about their function in brain development. Among 26 WDR genes assessed, we found 7 displaying a major impact in neuronal morphology when inactivated in mice. Remarkably, all seven genes showed corpus callosum defects, inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kannan M,Bayam E,Wagner C,Rinaldi B,Kretz PF,Tilly P,Roos M,McGillewie L,Bär S,Minocha S,Chevalier C,Po C,Sanger Mouse Genetics Project.,Chelly J,Mandel JL,Borgatti R,Piton A,Kinnear C,Loos B,Adams DJ,Hérault Y,

更新日期：2017-10-31 00:00:00

abstract：:Tiazofurin (2-beta-D-ribofuranosyl-4-thiazole-carboxamide; NSC 286193), an antitumor carbon-linked nucleoside that inhibits IMP dehydrogenase (IMP:NAD+ oxidoreductase; EC 1.1.1.205) and depletes guanylate levels, can activate the erythroid differentiation program of K-562 human leukemia cells. Tiazofurin-mediated cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olah E,Natsumeda Y,Ikegami T,Kote Z,Horanyi M,Szelenyi J,Paulik E,Kremmer T,Hollan SR,Sugar J

更新日期：1988-09-01 00:00:00

abstract：:Understanding the genetic networks that operate inside cells will require the dissection of interactions among network members. Here we describe a peptide aptamer isolated from a combinatorial library that distinguishes among such interactions. This aptamer binds to cyclin-dependent kinase 2 (Cdk2) and inhibits its ki...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cohen BA,Colas P,Brent R

更新日期：1998-11-24 00:00:00

abstract：:Phosphorylation of purified microtubule-associated proteins (MAPs) inhibits the rate and extent of MAP-stimulated microtubule assembly. The extent of microtubule assembly is reduced as a result of a decrease in the fraction of tubulin polymerized, without a significant change in the critical protein concentration. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jameson L,Caplow M

更新日期：1981-06-01 00:00:00

abstract：:Hereditary hemochromatosis (HH) is a common chronic human genetic disorder whose hallmark is systemic iron overload. Homozygosity for a mutation in the MHC class I heavy chain paralogue gene HFE has been found to be a primary cause of HH. However, many individuals homozygous for the defective allele of HFE do not deve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sproule TJ,Jazwinska EC,Britton RS,Bacon BR,Fleming RE,Sly WS,Roopenian DC

更新日期：2001-04-24 00:00:00