Diel cycling and long-term persistence of viruses in the ocean's euphotic zone.

abstract：:The chloroplast stromal proteins from spinach condense the two heterocyclic moieties of vitamin B1--4-methyl-5-(beta-hydroxyethyl)thiazole and 2-methyl-4-amino-5-hydroxymethylpyrimidine--in the presence of MgATP. We have taken advantage of this observation to study thiazole synthesis. We present data indicating that p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Julliard JH,Douce R

更新日期：1991-03-15 00:00:00

abstract：:Viperin is an IFN-inducible radical S-adenosylmethionine (SAM) enzyme that inhibits viral replication. We determined crystal structures of an anaerobically prepared fragment of mouse viperin (residues 45-362) complexed with S-adenosylhomocysteine (SAH) or 5'-deoxyadenosine (5'-dAdo) and l-methionine (l-Met). Viperin c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fenwick MK,Li Y,Cresswell P,Modis Y,Ealick SE

更新日期：2017-06-27 00:00:00

abstract：:Control over the fluorescence of supramolecular assemblies is crucial for the development of chemosensors and light-emitting materials. Consequently, the postsynthetic modification of supramolecular structures via host-guest interactions has emerged as an efficient strategy in recent years that allows the facile tunin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang M,Yin S,Zhang J,Zhou Z,Saha ML,Lu C,Stang PJ

更新日期：2017-03-21 00:00:00

abstract：:IFN-gamma is well known as the signature cytokine of CD4+ T helper 1, CD8+, and natural killer cells, but recent studies demonstrate that antigen-presenting cells, in particular dendritic cells (DCs), are another potent source for this proinflammatory cytokine. T-bet, a transcription factor that controls IFN-gamma exp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lugo-Villarino G,Maldonado-Lopez R,Possemato R,Penaranda C,Glimcher LH

更新日期：2003-06-24 00:00:00

abstract：:Bacterial outer membrane porins have a robust beta-barrel structure and therefore show potential for use as stochastic sensors based on single-molecule detection. The monomeric porin OmpG is especially attractive compared with multisubunit proteins because appropriate modifications of the pore can be easily achieved b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen M,Khalid S,Sansom MS,Bayley H

更新日期：2008-04-29 00:00:00

abstract：:Although potassium channels play a variety of roles in shaping the electrical properties of neurons, little is known about how these channels are constituted in neurons. To examine the assembly and physiological function of A-type K+ channels in mature differentiated neurons, we have developed a highly efficient gene ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaang BK,Pfaffinger PJ,Grant SG,Kandel ER,Furukawa Y

更新日期：1992-02-01 00:00:00

abstract：:Dengue virus (DENV) is the most prevalent human vector-borne viral disease. The force of infection (FoI), the rate at which susceptible individuals are infected in a population, is an important metric for infectious disease modeling. Understanding how and why the FoI of DENV changes over time is critical for developin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Katzelnick LC,Ben-Shachar R,Mercado JC,Rodriguez-Barraquer I,Elizondo D,Arguello S,Nuñez A,Ojeda S,Sanchez N,Lopez Mercado B,Gresh L,Burger-Calderon R,Kuan G,Gordon A,Balmaseda A,Harris E

更新日期：2018-10-16 00:00:00

abstract：:The present study examined the social skills of previously institutionalized, 8-y-old Romanian children from the Bucharest Early Intervention Project and the influence of attachment security and brain electrical activity (alpha power) on these skills. Participants included children randomized to an intervention involv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Almas AN,Degnan KA,Radulescu A,Nelson CA 3rd,Zeanah CH,Fox NA

更新日期：2012-10-16 00:00:00

abstract：:Myocardin and the myocardin-related transcription factors (MRTFs) A and B act as coactivators for serum response factor, which plays a key role in cardiovascular development. To determine the functions of MRTF-B in vivo, we generated MRTF-B mutant mice by targeted inactivation of the MRTF-B gene. We show that mice hom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oh J,Richardson JA,Olson EN

更新日期：2005-10-18 00:00:00

abstract：:This study demonstrates that significantly shortened telomeres are a hallmark of cardiomyocytes (CMs) from individuals with end-stage hypertrophic cardiomyopathy (HCM) or dilated cardiomyopathy (DCM) as a result of heritable defects in cardiac proteins critical to contractile function. Positioned at the ends of chromo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang ACY,Chang ACH,Kirillova A,Sasagawa K,Su W,Weber G,Lin J,Termglinchan V,Karakikes I,Seeger T,Dainis AM,Hinson JT,Seidman J,Seidman CE,Day JW,Ashley E,Wu JC,Blau HM

更新日期：2018-09-11 00:00:00

abstract：:The semaphorins comprise a large family of membrane-bound and secreted proteins, some of which have been shown to function in axon guidance. We have cloned a transmembrane semaphorin, Sema W, that belongs to the class IV subgroup of the semaphorin family. The mouse and rat forms of Sema W show 97% amino acid sequence ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Encinas JA,Kikuchi K,Chedotal A,de Castro F,Goodman CS,Kimura T

更新日期：1999-03-02 00:00:00

abstract：:Utilizing [3H]phorbol dibutyrate [P(Bu)2], we have developed an assay for high-affinity phorbol ester receptors in intact rat embryo fibroblasts. At 37 degrees C, binding of [3H]P(Bu)2 reached a maximum within 10 min and was rapidly reversible. The tumor promoters 12-O-tetradecanoyl-phorbol 13-acetate, teleocidin B, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horowitz AD,Greenebaum E,Weinstein IB

更新日期：1981-04-01 00:00:00

abstract：:Tooth decay (dental caries) is a widespread human disease caused by microbial biofilms. Streptococcus mutans, a biofilm-former, has been consistently associated with severe childhood caries; however, how this bacterium is spatially organized with other microorganisms in the oral cavity to promote disease remains unkno...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim D,Barraza JP,Arthur RA,Hara A,Lewis K,Liu Y,Scisci EL,Hajishengallis E,Whiteley M,Koo H

更新日期：2020-06-02 00:00:00

abstract：:Variable regions of Ig chains provide the antigen recognition portion of B-cell receptors and derivative antibodies. Ig heavy-chain variable region exons are assembled developmentally from V, D, J gene segments. Each variable region contains three antigen-contacting complementarity-determining regions (CDRs), with CDR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang JK,Wang C,Du Z,Meyers RM,Kepler TB,Neuberg D,Kwong PD,Mascola JR,Joyce MG,Bonsignori M,Haynes BF,Yeap LS,Alt FW

更新日期：2017-08-08 00:00:00

abstract：:The biodistribution profiles in mice of two pyrrole-imidazole polyamides were determined by PET. Pyrrole-imidazole polyamides are a class of small molecules that can be programmed to bind a broad repertoire of DNA sequences, disrupt transcription factor-DNA interfaces, and modulate gene expression pathways in cell cul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harki DA,Satyamurthy N,Stout DB,Phelps ME,Dervan PB

更新日期：2008-09-02 00:00:00

abstract：:The thylakoid compartments of plant chloroplasts are a vital destination for copper. Copper is needed to form holo-plastocyanin, which must shuttle electrons between photosystems to convert light into biologically useful chemical energy. Copper can bind tightly to proteins, so it has been hypothesized that copper part...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Banci L,Bertini I,Ciofi-Baffoni S,Kandias NG,Robinson NJ,Spyroulias GA,Su XC,Tottey S,Vanarotti M

更新日期：2006-05-30 00:00:00

abstract：:To complete the set of synthetic analogs of the three recognized types of active sites in iron-sulfur redox proteins, the compound (Et4N)[Fe((SCH2)2C6H4)2], derived from o-xylyl-alpha,alpha'-dithiol, has been prepared and its structure has been determined by x-ray diffraction. The bischelate anion contains a near-tetr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lane RW,Ibers JA,Frankel RB,Holm RH

更新日期：1975-08-01 00:00:00

abstract：:All life on Earth is built of organic molecules, so the primordial sources of reduced carbon remain a major open question in studies of the origin of life. A variant of the alkaline-hydrothermal-vent theory for life's emergence suggests that organics could have been produced by the reduction of CO2 via H2 oxidation, f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hudson R,de Graaf R,Strandoo Rodin M,Ohno A,Lane N,McGlynn SE,Yamada YMA,Nakamura R,Barge LM,Braun D,Sojo V

更新日期：2020-09-15 00:00:00

abstract：:Filipin, digitonin, and saponin react with membrane cholesterol to produce unique membrane alterations (sterol-specific complexes) that are easily discernible in freeze-fracture replicas. We have treated both noninnervated and innervated Xenopus embryonic muscle cells in culture with these agents. Freeze-fracture of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bridgman PC,Nakajima Y

更新日期：1981-02-01 00:00:00

abstract：:Uncoupling protein 1 (UCP1) catalyzes fatty acid-activated, purine nucleotide-sensitive proton leak across the mitochondrial inner membrane of brown adipose tissue to produce heat, and could help combat obesity and metabolic disease in humans. Studies over the last 30 years conclude that the protein is a dimer, bindin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee Y,Willers C,Kunji ER,Crichton PG

更新日期：2015-06-02 00:00:00

abstract：:Shoot architecture is critical for optimizing plant adaptation and productivity. In contrast with annuals, branching in perennials native to temperate and boreal regions must be coordinated with seasonal growth cycles. How branching is coordinated with seasonal growth is poorly understood. We identified key components...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maurya JP,Miskolczi PC,Mishra S,Singh RK,Bhalerao RP

更新日期：2020-05-26 00:00:00

abstract：:A crucial step in recent theories of human origins is the emergence of strong pair-bonding between males and females accompanied by a dramatic reduction in the male-to-male conflict over mating and an increased investment in offspring. How such a transition from promiscuity to pair-bonding could be achieved is puzzlin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gavrilets S

更新日期：2012-06-19 00:00:00

abstract：:A wide diversity of biological molecules are modified by the addition of sugar residues, and a large number of glycosyltransferases have been identified that are responsible for these reactions. Despite catalyzing closely related reactions, many of these transferases show little apparent sequence homology. By comparin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiggins CA,Munro S

更新日期：1998-07-07 00:00:00

abstract：:In enzyme catalysis, where exquisitely positioned functionality is the sine qua non, atomic coordinates for a Michaelis complex can provide powerful insights into activation of the substrate. We focus here on the initial proton transfer of the isomerization reaction catalyzed by triosephosphate isomerase and present t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jogl G,Rozovsky S,McDermott AE,Tong L

更新日期：2003-01-07 00:00:00

abstract：:A structural and functional model of bacterial nitric oxide reductase (NOR) has been designed by introducing two glutamates (Glu) and three histidines (His) in sperm whale myoglobin. X-ray structural data indicate that the three His and one Glu (V68E) residues bind iron, mimicking the putative Fe(B) site in NOR, while...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin YW,Yeung N,Gao YG,Miner KD,Tian S,Robinson H,Lu Y

更新日期：2010-05-11 00:00:00

abstract：:Monosynaptic excitatory connections between cerebellar granule and Purkinje cells were studied in dissociated cell cultures, and identification of the transmitter and the postsynaptic receptor at this synapse was pharmacologically investigated. The presynaptic granule cell and the postsynaptic Purkinje cell were volta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirano T,Hagiwara S

更新日期：1988-02-01 00:00:00

abstract：:Hereditary hemochromatosis (HH) is a common chronic human genetic disorder whose hallmark is systemic iron overload. Homozygosity for a mutation in the MHC class I heavy chain paralogue gene HFE has been found to be a primary cause of HH. However, many individuals homozygous for the defective allele of HFE do not deve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sproule TJ,Jazwinska EC,Britton RS,Bacon BR,Fleming RE,Sly WS,Roopenian DC

更新日期：2001-04-24 00:00:00

abstract：:Transformation promotes genome plasticity in bacteria via RecA-driven homologous recombination. In the gram-positive human pathogen Streptococcus pneumoniae, the transformasome a multiprotein complex, internalizes, protects, and processes transforming DNA to generate chromosomal recombinants. Double-stranded DNA is in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quevillon-Cheruel S,Campo N,Mirouze N,Mortier-Barrière I,Brooks MA,Boudes M,Durand D,Soulet AL,Lisboa J,Noirot P,Martin B,van Tilbeurgh H,Noirot-Gros MF,Claverys JP,Polard P

更新日期：2012-09-11 00:00:00

abstract：:Most processing of sensation involves the cortical hemisphere opposite (contralateral) to the stimulated limb. Stroke patients can exhibit changes in the interhemispheric balance of sensory signal processing. It is unclear whether these changes are the result of poststroke rewiring and experience, or whether they coul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohajerani MH,Aminoltejari K,Murphy TH

更新日期：2011-05-31 00:00:00

abstract：:Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Armache JP,Jarasch A,Anger AM,Villa E,Becker T,Bhushan S,Jossinet F,Habeck M,Dindar G,Franckenberg S,Marquez V,Mielke T,Thomm M,Berninghausen O,Beatrix B,Söding J,Westhof E,Wilson DN,Beckmann R

更新日期：2010-11-16 00:00:00